iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Tae-125455

Ensembl Protein ID

TRIAE_CS42_5DL_TGACv1_436752_AA1463090.3

UniProt Accession

A0A1D5ZYQ2; A0A1D5ZYQ2_WHEAT

Protein Name

NEDD8-activating enzyme E1 regulatory subunit

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
TRIAE_CS42_5DL_TGACv1_436752_AA1463090	TRIAE_CS42_5DL_TGACv1_436752_AA1463090.1	TRIAE_CS42_5DL_TGACv1_436752_AA1463090.1
TRIAE_CS42_5DL_TGACv1_436752_AA1463090	TRIAE_CS42_5DL_TGACv1_436752_AA1463090.2	TRIAE_CS42_5DL_TGACv1_436752_AA1463090.2
TRIAE_CS42_5DL_TGACv1_436752_AA1463090	TRIAE_CS42_5DL_TGACv1_436752_AA1463090.3	TRIAE_CS42_5DL_TGACv1_436752_AA1463090.3

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	4.90e-101	341.6	21	532

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedv 69
           YDRQ+r+wG+++q++l++a+++l+++g++G+E lKnLvl+G+gs+tvvD+++ve s+l+++FL+++   
   Q: 21   YDRQLRIWGDQGQAALEKASICLLNCGPTGTEALKNLVLGGIGSVTVVDDSKVEPSDLGNNFLLDEGCL 89
           ********************************************************************* PP
   S: 70   gksKAevaaealkelnpdvnveaheesiteleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpl 137
           g+s+A++ + +l+eln+ v+++ +ees+ +  ++++ Ff++f+vV++++ ++++ +k++ +c +a++ l
   Q: 90   GHSRAKSICSFLQELNDAVKAKYVEESVATMIDTNPsFFSEFTVVIATQLPESSLLKLDGICGSANIVL 158
           ********************************************************************* PP
   S: 138  iesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpn.........aaehtiewavlfn 194
           + ++++Gl G vrv+ik++ +++s++d   d++++ +P+ +lk++           +++++++++v++ 
   Q: 159  VAARSYGLTGLVRVSIKEHCVIESKPDhflDDLRLHNPWTELKQFAKsidicdkdaVVHKHTPYIVILV 227
           *********************************************88788888888************* PP
   S: 195  klleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfec 262
            l+e++a +++++ +++++ek+e+kd++++ + + de+n++ea+e + k   +  i++ i+q++++ + 
   Q: 228  RLAEKWADAHDGQLPSTRQEKREFKDLIRAHMLNVDEDNYKEAVESSYKVSLTPGISNeIRQIIDD-SS 295
           ******************************************************************.55 PP
   S: 263  divtkskspfwv......................................................... 274
            +v+ s+s+fwv                                                         
   Q: 296  SEVNFSSSDFWVlvsalkefitnegngelplegtipdmtslteyyvslqkiyqakaesdclamehrvks 364
           599****************************************************************** PP
   S: 275  ..................sfcsnaealq...........vpekvekdeevvkasap.....lylllral 309
                             +fc+n+++l+           + +   + +++++++++     +y+llra+
   Q: 365  ilkrigrdpesisrayikTFCKNTRKLKvcryrsmeeefSSPALSEVQKYFADEDScyamnFYVLLRAV 433
           ****************************666665555542244444444444444467888******** PP
   S: 310  erfekkkgrkpgelsfekddds....avdlvtaaanlraeslgiep.kldddlikeiagniipaiattn 373
           +r+++ ++r pg ++   +  +    +++l++aa +   +++g+++  l++dl++e+++++ ++i++++
   Q: 434  DRLAASYSRLPGIFDSIGSTSEivedVPRLKAAAVSVL-SDMGLKGaSLSEDLVTEVCRFAGAEIHPVA 501
           ***********887765555554555666666666555.****************************** PP
   S: 374  avvgGlaaqEviKvvsgkfeplknfflfdae 404
           a++gG+a+qEviK+v+++f+pl+ +f+f+++
   Q: 502  AFIGGVASQEVIKLVTKQFVPLNGTFIFNGI 532
           ****************************986 PP

Organism

Triticum aestivum

Functional Description
(View)

Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.

Protein Sequence
(Fasta)

MAAAPSASAS AVAVAEPKTK YDRQLRIWGD QGQAALEKAS ICLLNCGPTG TEALKNLVLG 60
GIGSVTVVDD SKVEPSDLGN NFLLDEGCLG HSRAKSICSF LQELNDAVKA KYVEESVATM 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CACTTCCACT TCCGCTCCTC GCTTTATTCC TCGGGGGCGC CGGTGAGGAG GCGGCGCGGC 60
CCGGCGCGGC GAGGCGGAAA CCCCCACCGC CGCACAGGGA AGAGACTACG CCTCCGCAGC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0019781--F:NEDD8 activating enzyme activity
GO:0045116--P:protein neddylation

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Bgr-012111	Blumeria graminis	33.27	2.00e-64	240.00
IUUC-Cme-027210	Cyanidioschyzon merolae	25.65	9.00e-16	79.30
IUUC-Mvi-060221	Microbotryum violaceum	33.78	1.00e-71	265.00
IUUC-Olu-087625	Ostreococcus lucimarinus	30.29	4.00e-61	229.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence