iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Ncr-068846

Ensembl Protein ID

EAA35003

UniProt Accession

Q7SDY2; ATG3_NEUCR

Genbank Protein ID

EAA35003.2

Protein Name

Autophagy-related protein 3; Autophagy-related E2-like conjugation enzyme atg3

Genbank Nucleotide ID

CM002236

Gene Name

apg-3; atg3; NCU01955

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
NCU01955	EAA35003	EAA35003

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E2/Autophagy_C	36.40	5.00e-32	135.00	IUUC-Ath-006802
E2/Autophagy_C	34.91	3.00e-33	140.00	IUUC-Hsa-046449
E2/Autophagy_C	38.40	9.00e-54	205.00	IUUC-Sce-106172

Active Site

Position(s)	Description	Evidence
238	Glycyl thioester intermediate	{ECO:0000250}

Organism

Neurospora crassa

Functional Description
(View)

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of apg-6/atg8. The atg12-apg-4/atg5 conjugate plays a role of an E3 and promotes the transfer of apg-6/atg8 from apg-3/atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of apg-6/atg8. The formation of the apg-6/atg8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The apg-6/atg8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).

Protein Sequence
(Fasta)

MNFLRSTAAT LLDKYTPVSH TSTFRNTGQI TPEEFVAAGD YLTFKFPSWS WADADSPSKR 60
LPFLPPGKQF LVTRHVPCHR RLNDDFAGDA GHEEALVEGN KGGADDDGWL RTGSMTSSQP 120

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Nucleotide Sequence
(Fasta)

GCGCTTTATC GGACAGGCCC CCGCACCTTC ACTCCAGGCA CCCGACCTCA TTGAACAGCG 60
ACAGCCAGGA CACGGGCGTG TTGCGCAAGC TTGGCGCCAT CCCGTCATCC ATATCATACC 120

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Sequence Source

Ensembl

Keyword

KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0833--Ubl conjugation pathway

Interpro

IPR007135--Autophagy-rel_prot_3
IPR019461--Autophagy-rel_prot_3_C
IPR007134--Autophagy-rel_prot_3_N

Pfam

PF03987--Autophagy_act_C
PF10381--Autophagy_C
PF03986--Autophagy_N

Gene Ontology

GO:0000153--C:cytoplasmic ubiquitin ligase complex
GO:0005829--C:cytosol
GO:0019776--F:Atg8 ligase activity
GO:0000045--P:autophagosome assembly
GO:0006501--P:C-terminal protein lipidation
GO:0044805--P:late nucleophagy
GO:0000422--P:mitophagy
GO:0044804--P:nucleophagy
GO:0034727--P:piecemeal microautophagy of nucleus
GO:0006612--P:protein targeting to membrane

KEGG

ncr:NCU01955

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-001111	Aegilops tauschii	33.59	6.00e-31	126.00
IUUC-Aml-002081	Ailuropoda melanoleuca	32.87	1.00e-36	145.00
IUUC-Atr-002712	Amborella trichopoda	31.80	3.00e-29	120.00
IUUC-Apl-003956	Anas platyrhynchos	28.74	5.00e-16	76.60
IUUC-Aca-004578	Anolis carolinensis	33.45	6.00e-34	135.00
IUUC-Aly-005823	Arabidopsis lyrata	36.61	2.00e-35	140.00
IUUC-Ath-006802	Arabidopsis thaliana	36.22	7.00e-35	139.00
IUUC-Ago-007855	Ashbya gossypii	35.84	2.00e-56	210.00
IUUC-Acl-008147	Aspergillus clavatus	61.50	1.00e-105	374.00
IUUC-Afl-008483	Aspergillus flavus	64.62	1.00e-113	400.00
IUUC-Afu-008818	Aspergillus fumigatus	64.07	1.00e-117	414.00
IUUC-Ani-009481	Aspergillus nidulans	60.22	4.00e-100	356.00
IUUC-Ang-009800	Aspergillus niger	61.98	7.00e-110	388.00
IUUC-Aor-010150	Aspergillus oryzae	64.62	1.00e-113	400.00
IUUC-Ate-010545	Aspergillus terreus	62.75	6.00e-63	231.00
IUUC-Ame-011793	Astyanax mexicanus	34.86	8.00e-37	145.00
IUUC-Bgr-012167	Blumeria graminis	58.95	1.00e-101	360.00
IUUC-Bta-012695	Bos taurus	32.18	2.00e-35	140.00
IUUC-Bci-013972	Botrytis cinerea	63.74	2.00e-99	353.00
IUUC-Bdi-014756	Brachypodium distachyon	33.07	7.00e-33	132.00
IUUC-Bol-015091	Brassica oleracea	36.08	2.00e-33	134.00
IUUC-Bra-017220	Brassica rapa	38.55	1.00e-34	138.00
IUUC-Cel-018143	Caenorhabditis elegans	36.70	1.00e-36	144.00
IUUC-Cja-018991	Callithrix jacchus	33.69	2.00e-35	141.00
IUUC-Cfa-020070	Canis familiaris	32.53	7.00e-36	142.00
IUUC-Cpo-021860	Cavia porcellus	34.41	5.00e-36	142.00
IUUC-Cre-022948	Chlamydomonas reinhardtii	32.52	1.00e-36	145.00
IUUC-Csa-023881	Chlorocebus sabaeus	34.77	5.00e-36	142.00
IUUC-Cho-024852	Choloepus hoffmanni	41.96	3.00e-19	87.40
IUUC-Cin-025787	Ciona intestinalis	46.25	1.00e-17	82.00
IUUC-Csv-026369	Ciona savignyi	32.16	2.00e-32	130.00
IUUC-Cgl-026680	Colletotrichum gloeosporioides	70.89	1.00e-116	410.00
IUUC-Cne-026905	Cryptococcus neoformans	36.22	2.00e-20	91.70
IUUC-Dre-028811	Danio rerio	35.46	2.00e-36	144.00
IUUC-Dno-030081	Dasypus novemcinctus	34.52	8.00e-34	135.00
IUUC-Dor-030183	Dipodomys ordii	42.86	3.00e-20	90.10
IUUC-Dse-031539	Dothistroma septosporum	63.64	2.00e-39	154.00
IUUC-Dme-031805	Drosophila melanogaster	40.87	1.00e-20	92.00
IUUC-Ete-033171	Echinops telfairi	32.28	2.00e-34	137.00
IUUC-Eca-034083	Equus caballus	32.18	2.00e-35	140.00
IUUC-Eeu-034804	Erinaceus europaeus	33.69	1.00e-33	134.00
IUUC-Fca-036289	Felis catus	32.18	2.00e-35	140.00
IUUC-Fal-037060	Ficedula albicollis	33.81	8.00e-34	135.00
IUUC-Fox-038051	Fusarium oxysporum	70.34	2.00e-122	430.00
IUUC-Fso-038124	Fusarium solani	73.65	3.00e-117	412.00
IUUC-Gmo-039538	Gadus morhua	36.13	8.00e-39	152.00
IUUC-Ggr-039880	Gaeumannomyces graminis	63.76	2.00e-121	426.00
IUUC-Gga-041108	Gallus gallus	35.36	6.00e-34	135.00
IUUC-Gac-041558	Gasterosteus aculeatus	36.19	2.00e-36	144.00
IUUC-Gma-043969	Glycine max	33.47	3.00e-32	130.00
IUUC-Ggo-045071	Gorilla gorilla	34.77	7.00e-36	142.00
IUUC-Hsa-046449	Homo sapiens	34.77	7.00e-36	142.00
IUUC-Hvu-047451	Hordeum vulgare	33.60	3.00e-31	127.00
IUUC-Itr-048360	Ictidomys tridecemlineatus	34.89	1.00e-35	141.00
IUUC-Kpa-049096	Komagataella pastoris	39.48	6.00e-56	209.00
IUUC-Lch-049726	Latimeria chalumnae	34.31	9.00e-34	135.00
IUUC-Lpe-050734	Leersia perrieri	35.80	1.00e-36	145.00
IUUC-Loc-051983	Lepisosteus oculatus	36.79	1.00e-36	144.00
IUUC-Lma-053038	Leptosphaeria maculans	60.40	5.00e-103	365.00
IUUC-Laf-054510	Loxodonta africana	35.55	2.00e-33	134.00
IUUC-Mcc-054792	Macaca mulatta	34.77	5.00e-36	142.00
IUUC-Meu-055965	Macropus eugenii	49.37	1.00e-16	78.60
IUUC-Mor-057292	Magnaporthe oryzae	67.42	2.00e-122	429.00
IUUC-Mtr-058282	Medicago truncatula	34.52	5.00e-33	132.00
IUUC-Mla-059144	Melampsora laricipopulina	37.97	6.00e-26	109.00
IUUC-Mga-059509	Meleagris gallopavo	41.96	1.00e-19	89.00
IUUC-Mvi-060281	Microbotryum violaceum	38.42	6.00e-24	103.00
IUUC-Mmr-061788	Microcebus murinus	32.18	1.00e-35	141.00
IUUC-Mdo-062922	Monodelphis domestica	32.53	1.00e-35	141.00
IUUC-Mmu-064249	Mus musculus	31.83	1.00e-34	138.00
IUUC-Mac-064474	Musa acuminata	32.84	8.00e-31	125.00
IUUC-Mpu-065717	Mustela putorius furo	32.53	4.00e-35	139.00
IUUC-Mlu-067151	Myotis lucifugus	31.34	7.00e-29	119.00
IUUC-Nfi-068551	Neosartorya fischeri	64.07	4.00e-119	419.00
IUUC-Nle-069790	Nomascus leucogenys	34.77	7.00e-36	142.00
IUUC-Ont-072517	Oreochromis niloticus	34.41	2.00e-34	137.00
IUUC-Oan-073285	Ornithorhynchus anatinus	49.37	8.00e-17	78.60
IUUC-Ocu-074695	Oryctolagus cuniculus	34.41	2.00e-35	140.00
IUUC-Oba-075515	Oryza barthii	35.02	4.00e-36	143.00
IUUC-Obr-076787	Oryza brachyantha	35.69	5.00e-37	145.00
IUUC-Ogl-077440	Oryza glaberrima	34.59	6.00e-37	145.00
IUUC-Ogu-078037	Oryza glumaepatula	35.69	4.00e-36	143.00
IUUC-Oin-079452	Oryza indica	35.29	4.00e-36	143.00
IUUC-Olo-080450	Oryza longistaminata	34.30	1.00e-34	137.00
IUUC-Ome-081514	Oryza meridionalis	35.55	5.00e-37	146.00
IUUC-Oni-082677	Oryza nivara	35.29	4.00e-36	143.00
IUUC-Opu-083925	Oryza punctata	35.55	1.00e-36	144.00
IUUC-Oru-084828	Oryza rufipogon	34.44	1.00e-34	138.00
IUUC-Osa-085937	Oryza sativa	35.29	4.00e-36	143.00
IUUC-Ola-086559	Oryzias latipes	46.43	4.00e-22	95.90
IUUC-Olu-087766	Ostreococcus lucimarinus	32.95	8.00e-37	145.00
IUUC-Oga-087983	Otolemur garnettii	34.64	9.00e-34	135.00
IUUC-Oar-089281	Ovis aries	32.18	2.00e-35	140.00
IUUC-Ptr-090983	Pan troglodytes	34.77	7.00e-36	142.00
IUUC-Pan-092457	Papio anubis	34.41	3.00e-35	140.00
IUUC-Psi-094046	Pelodiscus sinensis	35.25	3.00e-32	130.00
IUUC-Pno-094925	Phaeosphaeria nodorum	61.14	1.00e-103	367.00
IUUC-Ppa-095487	Physcomitrella patens	35.38	6.00e-34	135.00
IUUC-Pfo-096968	Poecilia formosa	43.75	8.00e-21	92.40
IUUC-Pab-098621	Pongo abelii	32.53	1.00e-34	138.00
IUUC-Pop-100007	Populus trichocarpa	34.63	1.00e-32	131.00
IUUC-Pca-100590	Procavia capensis	46.07	4.00e-17	80.10
IUUC-Ppe-101657	Prunus persica	34.87	3.00e-33	134.00
IUUC-Pva-102446	Pteropus vampyrus	32.17	2.00e-34	137.00
IUUC-Pgr-103604	Puccinia graminis	46.51	3.00e-19	87.80
IUUC-Ptt-103941	Puccinia triticina	35.29	1.00e-24	105.00
IUUC-Pte-104252	Pyrenophora teres	60.34	1.00e-103	367.00
IUUC-Pyt-104556	Pyrenophora triticirepentis	59.83	4.00e-103	365.00
IUUC-Rno-106025	Rattus norvegicus	34.89	2.00e-35	140.00
IUUC-Sce-106172	Saccharomyces cerevisiae	37.39	2.00e-52	197.00
IUUC-Sha-106858	Sarcophilus harrisii	31.93	1.00e-33	134.00
IUUC-Sja-107905	Schizosaccharomyces japonicus	31.46	1.00e-18	84.70
IUUC-Spo-108185	Schizosaccharomyces pombe	55.70	2.00e-17	81.30
IUUC-Smo-108883	Selaginella moellendorffii	31.02	7.00e-32	129.00
IUUC-Sit-110512	Setaria italica	48.10	9.00e-17	79.00
IUUC-Sly-111238	Solanum lycopersicum	30.56	9.00e-37	145.00
IUUC-Stu-112441	Solanum tuberosum	30.28	1.00e-35	141.00
IUUC-Sar-113039	Sorex araneus	45.56	4.00e-17	80.10
IUUC-Sbi-113776	Sorghum bicolor	46.51	2.00e-17	81.30
IUUC-Sre-115074	Sporisorium reilianum	45.78	6.00e-17	80.10
IUUC-Ssc-115262	Sus scrofa	32.53	3.00e-35	140.00
IUUC-Tgu-116790	Taeniopygia guttata	41.96	1.00e-19	89.00
IUUC-Tru-118160	Takifugu rubripes	44.64	7.00e-22	95.90
IUUC-Tsy-119145	Tarsius syrichta	23.75	2.00e-12	64.30
IUUC-Tni-119937	Tetraodon nigroviridis	31.34	1.00e-29	121.00
IUUC-Tca-121323	Theobroma cacao	34.26	2.00e-34	137.00
IUUC-Tre-121993	Trichoderma reesei	62.75	1.00e-102	364.00
IUUC-Tvi-122332	Trichoderma virens	62.82	1.00e-102	363.00
IUUC-Tae-124391	Triticum aestivum	33.86	8.00e-31	125.00
IUUC-Tur-126679	Triticum urartu	33.59	6.00e-31	126.00
IUUC-Tme-127159	Tuber melanosporum	44.85	7.00e-20	89.00
IUUC-Ttr-128261	Tursiops truncatus	32.84	5.00e-33	132.00
IUUC-Uma-129572	Ustilago maydis	33.84	3.00e-25	107.00
IUUC-Vda-129966	Verticillium dahliae	71.35	4.00e-131	459.00
IUUC-Vpa-130698	Vicugna pacos	27.93	8.00e-24	102.00
IUUC-Xtr-132878	Xenopus tropicalis	34.41	1.00e-35	141.00
IUUC-Xma-134017	Xiphophorus maculatus	43.75	9.00e-21	92.40
IUUC-Yli-134373	Yarrowia lipolytica	40.58	9.00e-57	212.00
IUUC-Zma-135222	Zea mays	46.51	4.00e-17	80.10

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence