iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

mRNA Expression
GEO	FFGED
Protein-protein Interaction
IID	iRefIndex	HINT	Mentha
Protein 3D Structure
PDB	MMDB	SCOP
Post-translational Modifications (PTMs)
CPLM	dbPAF	dbPTM	UniProt
BioGRID
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E1	E1_ThiF	1989885

Classification

Family	E-value	Score	Start	End
E1	1.60e-188	625.5	417	903

Active Site

Position(s)	Description	Evidence
600	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU10132, ECO:0000269\|PubMed:24816100}

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGv.....gsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnve..ahee 95
           YD Qi+++G + q+k+ ++kv+lvG g++GcE+lKn +l+G+     g i v+D+d +e+snlnrqFLfr++dvgk+K+evaaea+  +npd +++  a  +
   Q: 417  YDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLgsgsdGYIVVTDNDSIEKSNLNRQFLFRPKDVGKNKSEVAAEAVCAMNPDLKGKinAKID 518
           9*****************************************9999999*******************************************9876114557 PP
   S: 96   siteleeeiv...FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsddppqktiPfctlketpnaaehtiewa.... 190
           ++ +++eei+   F + +d+V +aldn++ar++v+r+c+ +r+pl+esgtlG++G+++vii+ lte+ys+s+dpp+k+iP+ctl+++pn+++hti wa    
   Q: 519  KVGPETEEIFndsFWESLDFVTNALDNVDARTYVDRRCVFYRKPLLESGTLGTKGNTQVIIPRLTESYSSSRDPPEKSIPLCTLRSFPNKIDHTIAWAkslf 620
           8888888888999**************************************************************************************999 PP
   S: 191  ........vlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttinsikqllksfecdivtkskspfwv.......... 274
                   + +n++l + ++ e++l+  +s + + +++ + s + s ++ nfe++i++a+ +f+k ++++ikqll +f++d  t+++ pfw+          
   Q: 621  qgyftdsaENVNMYLTQPNFVEQTLK--QSGDVKGVLESI-SDSLSSKPHNFEDCIKWARLEFEKKFNHDIKQLLFNFPKDAKTSNGEPFWSgakraptple 719
           99999999999999977777777776..345666666666.5555667****************************************************** PP
   S: 275  .................................................sfcsnae.alqvpekvekdeevvkasap.lylllralerfekkkgrkpgelsf 325
                                                            +f +na  ++qv++   ++ ++ +  ++ +  l+ +l   ++  g k + ++f
   Q: 720  fdiynndhfhfvvagaslraynygiksddsnskpnvdeyksvidhmiipEFTPNANlKIQVNDDDPDPNANAANGSDeIDQLVSSLPDPSTLAGFKLEPVDF 821
           ******************************888888877777777777766666543555655555555555544448************************ PP
   S: 326  ekddds..avdlvtaaanlraeslgiepkldddlikeiagniipaiattnavvgGlaaqEviKvvsg..kfeplknfflfdae 404
           ekddd   +++++ta++n ra+++ ie+ +d++++k iag+iipaiatt++ v Gl+++E++K++++  + e++kn f+++a+
   Q: 822  EKDDDTnhHIEFITACSNCRAQNYFIET-ADRQKTKFIAGRIIPAIATTTSLVTGLVNLELYKLIDNktDIEQYKNGFVNLAL 903
           *****999********************.**************************************777888*****99875 PP

Organism

Saccharomyces cerevisiae

Functional Description
(View)

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

Protein Sequence
(Fasta)

MSSNNSGLSA AGEIDESLYS RQLYVLGKEA MLKMQTSNVL ILGLKGLGVE IAKNVVLAGV 60
KSMTVFDPEP VQLADLSTQF FLTEKDIGQK RGDVTRAKLA ELNAYVPVNV LDSLDDVTQL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGAGCAGCA ATAATAGTGG TCTCTCTGCC GCCGGAGAAA TCGATGAAAG TCTTTATTCT 60
CGTCAACTTT ATGTGTTGGG TAAGGAAGCA ATGTTGAAAA TGCAAACCTC CAACGTATTA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-1017--Isopeptide bond
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR032420--E1_4HB
IPR032418--E1_FCCH
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR018965--Ub-activating_enz_E1_C
IPR023318--Ub_act_enz_dom_a
IPR019572--UBA_E1_Cys
IPR018075--UBQ-activ_enz_E1
IPR018074--UBQ-activ_enz_E1_CS
IPR033127--UBQ-activ_enz_E1_Cys_AS
IPR000011--UBQ/SUMO-activ_enz_E1-like

PROSITE

PS00536--UBIQUITIN_ACTIVAT_1
PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF16191--E1_4HB
PF16190--E1_FCCH
PF09358--E1_UFD
PF00899--ThiF
PF10585--UBA_e1_thiolCys

PRINTS

PR01849--UBIQUITINACT

SMART

SM00985--UBA_e1_C

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0004839--F:ubiquitin activating enzyme activity
GO:0016567--P:protein ubiquitination

KEGG

sce:YKL210W

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000456	Aegilops tauschii	44.88	0.00e+00	842.00
IUUC-Aml-001311	Ailuropoda melanoleuca	51.89	0.00e+00	1052.00
IUUC-Atr-002684	Amborella trichopoda	44.67	0.00e+00	845.00
IUUC-Apl-004158	Anas platyrhynchos	42.22	0.00e+00	695.00
IUUC-Aca-004620	Anolis carolinensis	52.25	0.00e+00	1042.00
IUUC-Aly-005515	Arabidopsis lyrata	45.02	0.00e+00	870.00
IUUC-Ath-006891	Arabidopsis thaliana	45.03	0.00e+00	872.00
IUUC-Ago-007873	Ashbya gossypii	73.75	0.00e+00	1537.00
IUUC-Acl-008181	Aspergillus clavatus	60.94	0.00e+00	1225.00
IUUC-Afl-008455	Aspergillus flavus	60.88	0.00e+00	1181.00
IUUC-Afu-009065	Aspergillus fumigatus	60.62	0.00e+00	1238.00
IUUC-Ani-009371	Aspergillus nidulans	60.10	0.00e+00	1177.00
IUUC-Ang-009737	Aspergillus niger	60.08	0.00e+00	1206.00
IUUC-Aor-010007	Aspergillus oryzae	60.88	0.00e+00	1180.00
IUUC-Ate-010466	Aspergillus terreus	59.94	0.00e+00	1237.00
IUUC-Ame-010933	Astyanax mexicanus	51.72	0.00e+00	979.00
IUUC-Bgr-012281	Blumeria graminis	58.20	0.00e+00	1168.00
IUUC-Bta-012767	Bos taurus	52.40	0.00e+00	1068.00
IUUC-Bci-013721	Botrytis cinerea	60.80	0.00e+00	1171.00
IUUC-Bdi-014009	Brachypodium distachyon	43.52	0.00e+00	838.00
IUUC-Bol-015665	Brassica oleracea	44.83	0.00e+00	858.00
IUUC-Bra-017685	Brassica rapa	45.41	0.00e+00	879.00
IUUC-Cel-018260	Caenorhabditis elegans	42.73	0.00e+00	758.00
IUUC-Cja-019152	Callithrix jacchus	52.09	0.00e+00	1065.00
IUUC-Cfa-020622	Canis familiaris	51.17	0.00e+00	1009.00
IUUC-Cpo-022526	Cavia porcellus	52.66	0.00e+00	1048.00
IUUC-Cre-022702	Chlamydomonas reinhardtii	43.42	0.00e+00	758.00
IUUC-Csa-023780	Chlorocebus sabaeus	52.38	0.00e+00	1060.00
IUUC-Cho-024682	Choloepus hoffmanni	40.00	4.00e-94	337.00
IUUC-Cin-025790	Ciona intestinalis	48.88	0.00e+00	1001.00
IUUC-Csv-026084	Ciona savignyi	48.55	0.00e+00	986.00
IUUC-Cgl-026512	Colletotrichum gloeosporioides	59.92	0.00e+00	1233.00
IUUC-Cne-026960	Cryptococcus neoformans	55.71	0.00e+00	1092.00
IUUC-Cme-027290	Cyanidioschyzon merolae	40.31	0.00e+00	764.00
IUUC-Dre-028149	Danio rerio	51.72	0.00e+00	1005.00
IUUC-Dno-028884	Dasypus novemcinctus	52.18	0.00e+00	1058.00
IUUC-Dor-030229	Dipodomys ordii	50.34	0.00e+00	1004.00
IUUC-Dse-031524	Dothistroma septosporum	57.99	0.00e+00	1165.00
IUUC-Dme-032045	Drosophila melanogaster	49.46	0.00e+00	967.00
IUUC-Ete-033124	Echinops telfairi	40.35	1.00e-138	485.00
IUUC-Eca-033330	Equus caballus	52.28	0.00e+00	1058.00
IUUC-Eeu-035323	Erinaceus europaeus	45.12	1.00e-161	562.00
IUUC-Fca-036255	Felis catus	52.33	0.00e+00	1058.00
IUUC-Fal-036848	Ficedula albicollis	42.31	0.00e+00	697.00
IUUC-Fox-038007	Fusarium oxysporum	61.33	0.00e+00	662.00
IUUC-Fso-038339	Fusarium solani	59.22	0.00e+00	1150.00
IUUC-Gmo-039338	Gadus morhua	49.32	0.00e+00	956.00
IUUC-Ggr-039823	Gaeumannomyces graminis	59.22	0.00e+00	1224.00
IUUC-Gga-040747	Gallus gallus	42.81	0.00e+00	700.00
IUUC-Gac-041773	Gasterosteus aculeatus	50.10	0.00e+00	962.00
IUUC-Gma-043198	Glycine max	45.39	0.00e+00	863.00
IUUC-Ggo-045336	Gorilla gorilla	52.47	0.00e+00	1046.00
IUUC-Hsa-045982	Homo sapiens	52.75	0.00e+00	1046.00
IUUC-Hvu-047418	Hordeum vulgare	45.56	0.00e+00	845.00
IUUC-Itr-048460	Ictidomys tridecemlineatus	52.28	0.00e+00	1061.00
IUUC-Kpa-049112	Komagataella pastoris	70.12	0.00e+00	1417.00
IUUC-Lch-050371	Latimeria chalumnae	51.86	0.00e+00	1031.00
IUUC-Lpe-051299	Leersia perrieri	44.27	0.00e+00	859.00
IUUC-Loc-052936	Lepisosteus oculatus	52.05	0.00e+00	1021.00
IUUC-Lma-053315	Leptosphaeria maculans	59.92	0.00e+00	1269.00
IUUC-Laf-053948	Loxodonta africana	51.99	0.00e+00	1033.00
IUUC-Mcc-055843	Macaca mulatta	52.28	0.00e+00	1059.00
IUUC-Meu-056258	Macropus eugenii	51.31	0.00e+00	1041.00
IUUC-Mor-057269	Magnaporthe oryzae	60.82	0.00e+00	1215.00
IUUC-Mpo-057435	Magnaporthe poae	59.39	0.00e+00	1192.00
IUUC-Mtr-058572	Medicago truncatula	46.11	0.00e+00	857.00
IUUC-Mla-059176	Melampsora laricipopulina	56.70	0.00e+00	1075.00
IUUC-Mga-059721	Meleagris gallopavo	43.02	0.00e+00	686.00
IUUC-Mvi-060437	Microbotryum violaceum	54.64	0.00e+00	1082.00
IUUC-Mmr-061405	Microcebus murinus	51.50	0.00e+00	1034.00
IUUC-Mdo-062032	Monodelphis domestica	49.66	0.00e+00	992.00
IUUC-Mmu-063280	Mus musculus	52.50	0.00e+00	1053.00
IUUC-Mac-065505	Musa acuminata	45.60	0.00e+00	862.00
IUUC-Mpu-066409	Mustela putorius furo	52.23	0.00e+00	1067.00
IUUC-Mlu-067217	Myotis lucifugus	52.16	0.00e+00	1038.00
IUUC-Nfi-068407	Neosartorya fischeri	60.43	0.00e+00	1212.00
IUUC-Ncr-068698	Neurospora crassa	60.93	0.00e+00	1234.00
IUUC-Nle-070072	Nomascus leucogenys	50.24	0.00e+00	974.00
IUUC-Opr-070715	Ochotona princeps	34.83	5.00e-161	559.00
IUUC-Ont-071671	Oreochromis niloticus	51.62	0.00e+00	998.00
IUUC-Oan-073268	Ornithorhynchus anatinus	41.32	0.00e+00	648.00
IUUC-Ocu-074544	Oryctolagus cuniculus	51.74	0.00e+00	1062.00
IUUC-Oba-075120	Oryza barthii	45.42	0.00e+00	850.00
IUUC-Obr-076557	Oryza brachyantha	45.36	0.00e+00	833.00
IUUC-Ogl-077887	Oryza glaberrima	45.42	0.00e+00	850.00
IUUC-Ogu-078305	Oryza glumaepatula	43.93	0.00e+00	848.00
IUUC-Oin-080154	Oryza indica	43.83	0.00e+00	845.00
IUUC-Olo-081089	Oryza longistaminata	44.02	0.00e+00	851.00
IUUC-Ome-081942	Oryza meridionalis	45.42	0.00e+00	850.00
IUUC-Oni-082365	Oryza nivara	45.42	0.00e+00	850.00
IUUC-Opu-083977	Oryza punctata	45.13	0.00e+00	846.00
IUUC-Oru-084469	Oryza rufipogon	44.02	0.00e+00	849.00
IUUC-Osa-085949	Oryza sativa	45.52	0.00e+00	855.00
IUUC-Ola-087002	Oryzias latipes	50.59	0.00e+00	991.00
IUUC-Olu-087687	Ostreococcus lucimarinus	42.55	0.00e+00	740.00
IUUC-Oga-088594	Otolemur garnettii	52.51	0.00e+00	1039.00
IUUC-Oar-090068	Ovis aries	52.35	0.00e+00	1063.00
IUUC-Ptr-090609	Pan troglodytes	43.00	0.00e+00	707.00
IUUC-Pan-092114	Papio anubis	52.28	0.00e+00	1060.00
IUUC-Psi-093489	Pelodiscus sinensis	42.87	0.00e+00	705.00
IUUC-Pma-094214	Petromyzon marinus	49.11	4.00e-38	148.00
IUUC-Pno-095154	Phaeosphaeria nodorum	59.61	0.00e+00	1249.00
IUUC-Ppa-095227	Physcomitrella patens	46.49	0.00e+00	845.00
IUUC-Pfo-097184	Poecilia formosa	50.49	0.00e+00	981.00
IUUC-Pab-097861	Pongo abelii	52.38	0.00e+00	1033.00
IUUC-Pop-098934	Populus trichocarpa	45.11	0.00e+00	880.00
IUUC-Pca-100347	Procavia capensis	51.10	0.00e+00	882.00
IUUC-Ppe-102012	Prunus persica	44.28	0.00e+00	851.00
IUUC-Pva-102584	Pteropus vampyrus	52.47	0.00e+00	1093.00
IUUC-Pgr-103336	Puccinia graminis	54.39	0.00e+00	1010.00
IUUC-Ptt-103939	Puccinia triticina	54.21	0.00e+00	1004.00
IUUC-Pte-104200	Pyrenophora teres	61.09	0.00e+00	1280.00
IUUC-Pyt-104441	Pyrenophora triticirepentis	60.15	0.00e+00	1269.00
IUUC-Rno-105462	Rattus norvegicus	52.23	0.00e+00	1052.00
IUUC-Sha-107142	Sarcophilus harrisii	51.71	0.00e+00	1040.00
IUUC-Sja-107841	Schizosaccharomyces japonicus	57.71	0.00e+00	1178.00
IUUC-Spo-108034	Schizosaccharomyces pombe	58.54	0.00e+00	1174.00
IUUC-Ssl-108497	Sclerotinia sclerotiorum	60.43	0.00e+00	1191.00
IUUC-Smo-109211	Selaginella moellendorffii	46.13	0.00e+00	830.00
IUUC-Sit-110281	Setaria italica	45.57	0.00e+00	864.00
IUUC-Sly-110914	Solanum lycopersicum	44.94	0.00e+00	884.00
IUUC-Stu-112109	Solanum tuberosum	44.65	0.00e+00	884.00
IUUC-Sar-113737	Sorex araneus	52.35	0.00e+00	1056.00
IUUC-Sbi-114089	Sorghum bicolor	45.57	0.00e+00	868.00
IUUC-Sre-115148	Sporisorium reilianum	55.22	0.00e+00	1073.00
IUUC-Ssc-115777	Sus scrofa	52.28	0.00e+00	1057.00
IUUC-Tgu-116565	Taeniopygia guttata	42.41	0.00e+00	699.00
IUUC-Tru-118742	Takifugu rubripes	50.83	0.00e+00	962.00
IUUC-Tsy-119436	Tarsius syrichta	37.78	9.00e-167	578.00
IUUC-Tni-120401	Tetraodon nigroviridis	51.13	0.00e+00	969.00
IUUC-Tca-121672	Theobroma cacao	46.11	0.00e+00	884.00
IUUC-Tre-122009	Trichoderma reesei	59.16	0.00e+00	1135.00
IUUC-Tvi-122259	Trichoderma virens	59.57	0.00e+00	1139.00
IUUC-Tae-125676	Triticum aestivum	43.40	0.00e+00	843.00
IUUC-Tur-126330	Triticum urartu	44.98	0.00e+00	846.00
IUUC-Tme-127055	Tuber melanosporum	58.38	0.00e+00	1149.00
IUUC-Tbe-127487	Tupaia belangeri	53.86	4.00e-148	516.00
IUUC-Ttr-128331	Tursiops truncatus	52.38	0.00e+00	1054.00
IUUC-Uma-129387	Ustilago maydis	55.04	0.00e+00	1076.00
IUUC-Vda-129977	Verticillium dahliae	58.61	0.00e+00	1130.00
IUUC-Vpa-130716	Vicugna pacos	41.93	0.00e+00	697.00
IUUC-Vvi-130857	Vitis vinifera	44.61	0.00e+00	879.00
IUUC-Xtr-132026	Xenopus tropicalis	42.94	0.00e+00	751.00
IUUC-Xma-133805	Xiphophorus maculatus	50.78	0.00e+00	971.00
IUUC-Yli-134547	Yarrowia lipolytica	62.05	0.00e+00	1308.00
IUUC-Zma-135052	Zea mays	45.80	0.00e+00	870.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence