iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

UUCD2 ID

IUUC-Gma-043221

UUCD1 version

Ensembl Protein ID

UniProt Accession

Genbank Protein ID

Protein Name

NEDD8-activating enzyme E1 regulatory subunit

Genbank Nucleotide ID

CM000845

Gene Name

LOC100777973; GLYMA_12G098800

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
GLYMA12G10540	GLYMA12G10540.1	GLYMA12G10540.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	1.20e-114	385.4	8	514

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveahe 94
           YDRQ+r+wGe++q++l++++++l+++g++G+E+lKnLvl+GvgsitvvD+++ve  +l+++FL+++ + gksKA++++ +l+eln+ v+++++e
   Q: 8    YDRQLRIWGEQGQAALEKSSICLLNCGPTGSETLKNLVLGGVGSITVVDGSKVEAGDLGNNFLVDEASLGKSKAKCVCSFLQELNDAVKAKFVE 101
           ********************************************************************************************** PP
   S: 95   esiteleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpn... 181
           es+++l e+++ Ff++f++Vv+++  + ++ k++++c++ +v li ++++Gl G+vr+++k++ +++s+++   d++++++P+++lk +     
   Q: 102  ESPETLIETNPsFFSQFTLVVATQLMENSMIKLDQICREVNVMLIFARSYGLTGFVRISVKEHAVIESKPEhflDDLRLNNPWPKLKRFADdid 195
           *****************************************************************************************88888 PP
   S: 182  ......aaehtiewavlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllksfecdivtks 268
                 +a+++i+++v++ k+++e+a+++ ++ +l++eek+e+k+  k++++++de+n++eai  a+k fa++ i+   +q+l++ + ++v ++
   Q: 196  lnvqdpVAHKHIPYVVILVKMADEWAQSHGGRLPLTREEKKEFKEFLKAGMVAQDEDNYKEAIDSAFKVFAPQGISLeLQQILND-SSAEVDSN 288
           888899***********************************************************************9******9.78899*** PP
   S: 269  kspfwv...........................................................................sfcsnaealq... 284
           +s+fwv                                                                           sfc+na++l+   
   Q: 289  SSDFWVlvtalkdfianegggeaplegsipdmtssteqyvnlqniyhakaeadflvierlvrstlkkigrdsnsisksiikSFCKNARKLKvcr 382
           *******************************************************************************************666 PP
   S: 285  ........vpekvekdeevvkasap.....lylllralerfekkkgrkpgelsfekdddsavdlvtaaanlraeslgiep.kldddlikeiagn 364
                     ++  + +++++++++     +y+llra++rf++++++ pg+++  +d+d +++l+ +a  l   +lg+++  l +dli e++++
   Q: 383  yrliedefNSPNLPELQKYLTDEDYsiavgIYILLRAVDRFAANYNSFPGQFDSAMDED-IPRLKSTAIGLL-NDLGCNGaTLAEDLINEMCRF 474
           6665555444888888999999999999*******************************.******999988.********************* PP
   S: 365  iipaiattnavvgGlaaqEviKvvsgkfeplknfflfdae 404
           ++++++ ++a vgG+a+qEviK+++ +f+p+  +f+f+++
   Q: 475  GAAELHAVAALVGGIASQEVIKLITRQFVPMSGTFIFNGI 514
           *************************************986 PP

Organism

Glycine max

Functional Description
(View)

Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.

Protein Sequence
(Fasta)

MAEPKVKYDR QLRIWGEQGQ AALEKSSICL LNCGPTGSET LKNLVLGGVG SITVVDGSKV 60
EAGDLGNNFL VDEASLGKSK AKCVCSFLQE LNDAVKAKFV EESPETLIET NPSFFSQFTL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GTAGATACGG AATGATAATC CGTAAGCACA AGCAATTTTC TGGGTGCGCA ATACACAGCG 60
TTTGCAGCAA GCAAAGCAAG CTAACAGTAC TATAAATAGC GAACGAGTGA AAAAAAAGAG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0019781--F:NEDD8 activating enzyme activity
GO:0045116--P:protein neddylation

KEGG

gmx:100777973

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ago-007900	Ashbya gossypii	27.50	6.00e-38	151.00
IUUC-Bgr-012111	Blumeria graminis	33.33	2.00e-65	243.00
IUUC-Cel-018456	Caenorhabditis elegans	31.72	2.00e-74	273.00
IUUC-Dse-031536	Dothistroma septosporum	32.07	1.00e-73	270.00
IUUC-Dme-031683	Drosophila melanogaster	38.65	5.00e-101	361.00
IUUC-Fso-038387	Fusarium solani	31.18	3.00e-66	246.00
IUUC-Mla-059183	Melampsora laricipopulina	34.81	4.00e-87	315.00
IUUC-Ppa-095798	Physcomitrella patens	55.64	0.00e+00	637.00
IUUC-Pgr-103401	Puccinia graminis	29.03	1.00e-72	267.00
IUUC-Ptt-103889	Puccinia triticina	28.17	1.00e-61	230.00
IUUC-Sce-106397	Saccharomyces cerevisiae	29.67	6.00e-34	138.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence