iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Mmu-063280

UUCD1 version

UUC-MuM-01127

Ensembl Protein ID

ENSMUSP00000086626.4

UniProt Accession

Q02053; A6H6S6; P31253; Q542H8; UBA1_MOUSE

Genbank Protein ID

BAA01433.1; BAC40121.1; BAC40405.1; BAE25369.1; BAE42599.1; CAM23092.1; AAH58630.1; AAI45985.1; CAA44465.1; AAC52169.1; AAC52171.1

Protein Name

Ubiquitin-like modifier-activating enzyme 1; Ubiquitin-activating enzyme E1; Ubiquitin-activating enzyme E1 X; Ubiquitin-like modifier-activating enzyme 1 X

Genbank Nucleotide ID

D10576; AK088057; AK088528; AK143416; AK171667; AL807240; BC058630; BC145984; X62580; U09051; U09055

Gene Name

Uba1; Sbx; Ube1; Ube1ax; Ube1x

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000001924.15	ENSMUST00000089217.10	ENSMUSP00000086626.4

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	miRTarBase	microRNA	TRANSFAC
RepTar	RAID2
Protein-protein Interaction
IID	PINA	HINT	Mentha
Protein 3D Structure
PDB	MMDB
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
Phospho.ELM	UniProt	PHOSIDA	mUbiSiDa
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	2.10e-235	782.6	451	931

Active Site

Position(s)	Description	Evidence
632	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU10132}

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGv.....gsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkel 84
           YD+Q++++G++ qekl+++k++lvGag++GcEllKn++++G+     g++ v+Dmdt+e+snlnrqFLfr++dv k K+++aa+a++++
   Q: 451  YDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMIGLgcgegGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQM 539
           ***************************************************************************************** PP
   S: 85   npdvnveaheesiteleeeiv...FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsddppqkt 170
           np+++v++h++++ +++e+i+   Ff+++d+V++aldn++ar++++r+c+++r+pl+esgtlG++G+v+v+i+ lte+ys+s+dpp+k+
   Q: 540  NPYIQVTSHQNRVGPDTERIYdddFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKS 628
           ***************************************************************************************** PP
   S: 171  iPfctlketpnaaehtiewa............vlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafak 247
           iP+ctlk++pna+eht++wa            + +n++l ++++++e++ +l+ ++++e++++v+++l+ ++++++ +++++a++++++
   Q: 629  IPICTLKNFPNAIEHTLQWArdefeglfkqpaENVNQYL-TDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHT 716
           ***************************************.77888888999************************************** PP
   S: 248  ttinsikqllksfecdivtkskspfwv..........................................sfcsnaealqvp........ 286
           ++ n+i+qll++f++d++t+s++pfw+                                           + s+ +++qvp        
   Q: 717  QYCNNIRQLLHNFPPDQLTSSGAPFWSgpkrcphpltfdvnntlhldyvmaaanlfaqtygltgsqdraAVASLLQSVQVPeftpksgv 805
           ******************************************************************99977777777777755555555 PP
   S: 287  ekvekdeevvkasap.....lylllralerfekkkgrkpgelsfekddds..avdlvtaaanlraeslgiepkldddlikeiagniipa 368
           + +++d+e ++a+a+     l+ l+++l + +k +g k++ ++fekddds  ++d++ aa+nlrae+++i+p +d+++ k iag+iipa
   Q: 806  KIHVSDQELQSANASvddsrLEELKATLPSPDKLPGFKMYPIDFEKDDDSnfHMDFIVAASNLRAENYDISP-ADRHKSKLIAGKIIPA 893
           66666666666666688889****************************************************.**************** PP
   S: 369  iattnavvgGlaaqEviKvvsg..kfeplknfflfdae 404
           iatt+a+v Gl+++E++Kvv+g  + + +kn fl++a+
   Q: 894  IATTTAAVVGLVCLELYKVVQGhqQLDSYKNGFLNLAL 931
           **********************9999********9875 PP

Organism

Mus musculus

Functional Description
(View)

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1511901). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.

Protein Sequence
(Fasta)

MSSSPLSKKR RVSGPDPKPG SNCSPAQSAL SEVSSVPTNG MAKNGSEADI DESLYSRQLY 60
VLGHEAMKML QTSSVLVSGL RGLGVEIAKN IILGGVKAVT LHDQGTTQWA DLSSQFYLRE 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GAGGCGCAGA AGTAGAGAGA AATGCCCCTC TATTCAGGCG TCTATGGTAT ACCCCAGACC 60
CGGCCCTCTA AGGCCCCCGC GCTTCCGAGC TCCGCGCAAA CTCTGGTTCT TTTTGCACGA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0436--Ligase
KW-0496--Mitochondrion
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR032420--E1_4HB
IPR032418--E1_FCCH
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR018965--Ub-activating_enz_E1_C
IPR019572--UBA_E1_Cys
IPR018075--UBQ-activ_enz_E1
IPR018074--UBQ-activ_enz_E1_CS
IPR033127--UBQ-activ_enz_E1_Cys_AS
IPR000011--UBQ/SUMO-activ_enz_E1-like

PROSITE

PS00536--UBIQUITIN_ACTIVAT_1
PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF16191--E1_4HB
PF16190--E1_FCCH
PF09358--E1_UFD
PF00899--ThiF
PF10585--UBA_e1_thiolCys

PRINTS

PR01849--UBIQUITINACT

SMART

SM00985--UBA_e1_C

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0030057--C:desmosome
GO:0010008--C:endosome membrane
GO:0070062--C:extracellular exosome
GO:0000792--C:heterochromatin
GO:0005765--C:lysosomal membrane
GO:0005739--C:mitochondrion
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0030867--C:rough endoplasmic reticulum membrane
GO:0005524--F:ATP binding
GO:0003723--F:RNA binding
GO:0004839--F:ubiquitin activating enzyme activity
GO:0006974--P:cellular response to DNA damage stimulus

KEGG

mmu:22201

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001311	Ailuropoda melanoleuca	93.69	0.00e+00	2039.00
IUUC-Atr-002684	Amborella trichopoda	44.91	0.00e+00	879.00
IUUC-Aca-004620	Anolis carolinensis	79.94	0.00e+00	1773.00
IUUC-Ago-007873	Ashbya gossypii	54.38	0.00e+00	1065.00
IUUC-Acl-008181	Aspergillus clavatus	53.72	0.00e+00	1064.00
IUUC-Afl-008455	Aspergillus flavus	53.64	0.00e+00	1030.00
IUUC-Afu-009065	Aspergillus fumigatus	53.37	0.00e+00	1086.00
IUUC-Ani-009371	Aspergillus nidulans	53.29	0.00e+00	1053.00
IUUC-Ang-009737	Aspergillus niger	53.74	0.00e+00	1070.00
IUUC-Aor-010007	Aspergillus oryzae	53.64	0.00e+00	1030.00
IUUC-Ate-010466	Aspergillus terreus	53.49	0.00e+00	1096.00
IUUC-Ame-010933	Astyanax mexicanus	78.56	0.00e+00	1678.00
IUUC-Bgr-012281	Blumeria graminis	53.63	0.00e+00	1086.00
IUUC-Bta-012767	Bos taurus	94.90	0.00e+00	2068.00
IUUC-Bci-013721	Botrytis cinerea	53.92	0.00e+00	1025.00
IUUC-Bdi-014843	Brachypodium distachyon	44.18	0.00e+00	863.00
IUUC-Cel-018260	Caenorhabditis elegans	49.18	0.00e+00	958.00
IUUC-Cja-019152	Callithrix jacchus	95.46	0.00e+00	2076.00
IUUC-Cfa-020622	Canis familiaris	92.91	0.00e+00	1976.00
IUUC-Cpo-022526	Cavia porcellus	94.99	0.00e+00	2045.00
IUUC-Cre-022702	Chlamydomonas reinhardtii	44.93	0.00e+00	838.00
IUUC-Csa-023780	Chlorocebus sabaeus	95.27	0.00e+00	2073.00
IUUC-Cho-024896	Choloepus hoffmanni	84.10	6.00e-114	405.00
IUUC-Cin-025790	Ciona intestinalis	63.28	0.00e+00	1362.00
IUUC-Csv-026084	Ciona savignyi	60.63	0.00e+00	1348.00
IUUC-Cgl-026512	Colletotrichum gloeosporioides	54.12	0.00e+00	1108.00
IUUC-Cne-026960	Cryptococcus neoformans	55.63	0.00e+00	1124.00
IUUC-Cme-027290	Cyanidioschyzon merolae	43.95	0.00e+00	830.00
IUUC-Dre-028149	Danio rerio	78.38	0.00e+00	1706.00
IUUC-Dno-028884	Dasypus novemcinctus	94.33	0.00e+00	2061.00
IUUC-Dor-030229	Dipodomys ordii	90.74	0.00e+00	1960.00
IUUC-Dse-031524	Dothistroma septosporum	52.18	0.00e+00	1075.00
IUUC-Dme-032045	Drosophila melanogaster	60.43	0.00e+00	1274.00
IUUC-Ete-032544	Echinops telfairi	83.62	0.00e+00	862.00
IUUC-Eca-033330	Equus caballus	95.37	0.00e+00	2075.00
IUUC-Fca-036255	Felis catus	95.37	0.00e+00	2053.00
IUUC-Fal-037445	Ficedula albicollis	47.78	0.00e+00	894.00
IUUC-Fox-038007	Fusarium oxysporum	53.33	2.00e-166	579.00
IUUC-Fso-038339	Fusarium solani	54.05	0.00e+00	1066.00
IUUC-Gmo-039338	Gadus morhua	75.33	0.00e+00	1607.00
IUUC-Ggr-039823	Gaeumannomyces graminis	54.15	0.00e+00	1127.00
IUUC-Gga-040365	Gallus gallus	47.20	0.00e+00	813.00
IUUC-Gac-041773	Gasterosteus aculeatus	75.14	0.00e+00	1602.00
IUUC-Ggo-045336	Gorilla gorilla	95.75	0.00e+00	2058.00
IUUC-Hsa-045982	Homo sapiens	95.56	0.00e+00	2052.00
IUUC-Hvu-047418	Hordeum vulgare	45.49	0.00e+00	871.00
IUUC-Itr-048460	Ictidomys tridecemlineatus	95.56	0.00e+00	2081.00
IUUC-Kpa-049112	Komagataella pastoris	51.16	0.00e+00	1018.00
IUUC-Lch-050371	Latimeria chalumnae	79.60	0.00e+00	1767.00
IUUC-Lpe-051299	Leersia perrieri	43.89	0.00e+00	875.00
IUUC-Loc-052936	Lepisosteus oculatus	79.98	0.00e+00	1749.00
IUUC-Lma-053315	Leptosphaeria maculans	52.91	0.00e+00	1098.00
IUUC-Laf-053948	Loxodonta africana	92.53	0.00e+00	2010.00
IUUC-Mcc-055843	Macaca mulatta	95.56	0.00e+00	2084.00
IUUC-Meu-056258	Macropus eugenii	86.48	0.00e+00	1894.00
IUUC-Mor-057269	Magnaporthe oryzae	54.09	0.00e+00	1099.00
IUUC-Mpo-057435	Magnaporthe poae	54.20	0.00e+00	1093.00
IUUC-Mla-059176	Melampsora laricipopulina	56.05	0.00e+00	1103.00
IUUC-Mvi-060437	Microbotryum violaceum	53.39	0.00e+00	1068.00
IUUC-Mmr-061405	Microcebus murinus	93.19	0.00e+00	2012.00
IUUC-Mdo-062032	Monodelphis domestica	84.91	0.00e+00	1868.00
IUUC-Mac-065505	Musa acuminata	44.81	0.00e+00	876.00
IUUC-Mpu-066409	Mustela putorius furo	95.09	0.00e+00	2075.00
IUUC-Mlu-067217	Myotis lucifugus	93.76	0.00e+00	2041.00
IUUC-Nfi-068407	Neosartorya fischeri	53.67	0.00e+00	1065.00
IUUC-Ncr-068698	Neurospora crassa	54.86	0.00e+00	1115.00
IUUC-Nle-070072	Nomascus leucogenys	90.25	0.00e+00	1931.00
IUUC-Ont-071671	Oreochromis niloticus	77.82	0.00e+00	1682.00
IUUC-Oan-073306	Ornithorhynchus anatinus	48.87	0.00e+00	720.00
IUUC-Ocu-074544	Oryctolagus cuniculus	94.33	0.00e+00	2061.00
IUUC-Ogu-078305	Oryza glumaepatula	43.65	0.00e+00	871.00
IUUC-Oin-080154	Oryza indica	43.65	0.00e+00	870.00
IUUC-Olo-081089	Oryza longistaminata	43.65	0.00e+00	871.00
IUUC-Oru-084469	Oryza rufipogon	43.75	0.00e+00	874.00
IUUC-Ola-087002	Oryzias latipes	77.06	0.00e+00	1674.00
IUUC-Olu-087687	Ostreococcus lucimarinus	44.01	0.00e+00	816.00
IUUC-Oga-088594	Otolemur garnettii	95.00	0.00e+00	2041.00
IUUC-Oar-090068	Ovis aries	95.09	0.00e+00	2070.00
IUUC-Pan-092114	Papio anubis	95.65	0.00e+00	2085.00
IUUC-Pma-094214	Petromyzon marinus	58.14	1.00e-52	198.00
IUUC-Pno-095154	Phaeosphaeria nodorum	53.25	0.00e+00	1106.00
IUUC-Ppa-095416	Physcomitrella patens	45.28	0.00e+00	893.00
IUUC-Pfo-097184	Poecilia formosa	77.25	0.00e+00	1673.00
IUUC-Pab-097861	Pongo abelii	95.27	0.00e+00	2038.00
IUUC-Pca-100347	Procavia capensis	88.98	0.00e+00	1610.00
IUUC-Ppe-102012	Prunus persica	43.76	0.00e+00	889.00
IUUC-Pva-102584	Pteropus vampyrus	95.18	0.00e+00	2088.00
IUUC-Pgr-103336	Puccinia graminis	54.46	0.00e+00	1060.00
IUUC-Ptt-103939	Puccinia triticina	54.36	0.00e+00	1064.00
IUUC-Pte-104200	Pyrenophora teres	53.45	0.00e+00	1099.00
IUUC-Pyt-104441	Pyrenophora triticirepentis	52.34	0.00e+00	1090.00
IUUC-Rno-105462	Rattus norvegicus	98.20	0.00e+00	2098.00
IUUC-Sce-106245	Saccharomyces cerevisiae	52.31	0.00e+00	1085.00
IUUC-Sha-107142	Sarcophilus harrisii	86.20	0.00e+00	1903.00
IUUC-Sja-107841	Schizosaccharomyces japonicus	55.51	0.00e+00	1137.00
IUUC-Spo-108034	Schizosaccharomyces pombe	53.72	0.00e+00	1079.00
IUUC-Ssl-108497	Sclerotinia sclerotiorum	54.00	0.00e+00	1049.00
IUUC-Smo-109211	Selaginella moellendorffii	45.44	0.00e+00	877.00
IUUC-Sar-113737	Sorex araneus	94.52	0.00e+00	2051.00
IUUC-Sre-115148	Sporisorium reilianum	54.31	0.00e+00	1083.00
IUUC-Ssc-115777	Sus scrofa	95.47	0.00e+00	2071.00
IUUC-Tru-118742	Takifugu rubripes	76.96	0.00e+00	1618.00
IUUC-Tsy-119485	Tarsius syrichta	95.38	8.00e-171	593.00
IUUC-Tni-120401	Tetraodon nigroviridis	77.15	0.00e+00	1621.00
IUUC-Tre-122009	Trichoderma reesei	53.39	0.00e+00	1053.00
IUUC-Tvi-122259	Trichoderma virens	53.73	0.00e+00	1039.00
IUUC-Tme-127055	Tuber melanosporum	53.42	0.00e+00	1014.00
IUUC-Tbe-127487	Tupaia belangeri	94.03	0.00e+00	1023.00
IUUC-Ttr-128331	Tursiops truncatus	94.42	0.00e+00	2043.00
IUUC-Uma-129387	Ustilago maydis	54.28	0.00e+00	1077.00
IUUC-Vda-129977	Verticillium dahliae	53.27	0.00e+00	1011.00
IUUC-Vvi-130857	Vitis vinifera	44.74	0.00e+00	894.00
IUUC-Xtr-132026	Xenopus tropicalis	64.07	0.00e+00	1278.00
IUUC-Xma-133805	Xiphophorus maculatus	76.48	0.00e+00	1643.00
IUUC-Yli-134547	Yarrowia lipolytica	54.15	0.00e+00	1112.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence