PTM - CPLM Gene Name UniProt ID Type Position Code CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 99 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 588 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 694 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 735 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 431 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 330 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 415 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 441 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 557 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 718 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 720 K CDC27; ANAPC3; D0S1430E; D17S978E P30260 Ubiquitination 730 K

PTM - dbPAF Gene Name UniProt ID Position Type Species CDC27; ANAPC3; D0S1430E; D17S978E P30260 154 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 177 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 183 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 186 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 192 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 194 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 200 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 203 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 205 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 209 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 219 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 220 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 222 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 241 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 244 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 252 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 264 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 276 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 279 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 289 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 291 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 296 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 304 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 312 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 313 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 320 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 327 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 329 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 334 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 336 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 339 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 343 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 359 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 364 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 366 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 368 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 369 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 378 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 379 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 383 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 384 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 386 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 387 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 388 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 389 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 393 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 426 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 430 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 434 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 435 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 438 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 444 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 446 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 553 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 578 S Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 68 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 691 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 740 Y Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 809 T Homo sapiens(Human) CDC27; ANAPC3; D0S1430E; D17S978E P30260 821 S Homo sapiens(Human)

PTM - PhosSNP Gene Name dbSNP ID Gene Mutation AA Mutation Mutation Position Code Peptide Score Modification Type CDC27; ANAPC3; D0S1430E; D17S978E rs11491191 TCC->TTC S->F 441 S EITKLDSSIISEGKI 435 Phosphorylation: CDK1; PLK1 TypeI- CDC27; ANAPC3; D0S1430E; D17S978E rs3952953 AGC->GGC S->G 432 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeI- CDC27; ANAPC3; D0S1430E; D17S978E rs4018103 TCC->TTC S->F 441 S EITKLDSSIISEGKI 435 Phosphorylation: CDK1; PLK1 TypeI- CDC27; ANAPC3; D0S1430E; D17S978E rs11491191 TCC->TTC S->F 435 S EITKLDSSIISEGKI 435 Phosphorylation: CDK1; PLK1 TypeI- CDC27; ANAPC3; D0S1430E; D17S978E rs3952953 AGC->GGC S->G 426 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeI- CDC27; ANAPC3; D0S1430E; D17S978E rs4018103 TCC->TTC S->F 435 S EITKLDSSIISEGKI 435 Phosphorylation: CDK1; PLK1 TypeI- CDC27; ANAPC3; D0S1430E; D17S978E rs381939 CCA->ACA P->T 312 S PPVIDVPSTGAPSKK 312 Phosphorylation: CDK1 TypeII- CDC27; ANAPC3; D0S1430E; D17S978E rs381939 CCA->ACA P->T 312 S PPVIDVPSTGAPSKK 312 Phosphorylation: CDK1 TypeII- CDC27; ANAPC3; D0S1430E; D17S978E rs11491191 TCC->TTC S->F 440 S LEITKLDSSIISEGK 434 Phosphorylation: PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs1984700 ACA->ATA T->I 370 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2311272 AAT->AGT N->S 452 T EGKISTITPQIQAFN 446 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2311273 GAC->TAC D->Y 440 S LEITKLDSSIISEGK 434 Phosphorylation: PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2311273 GAC->TAC D->Y 441 S EITKLDSSIISEGKI 435 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2311275 CAA->AAA Q->K 432 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369155 CGA->CAA R->Q 349 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369156 AAT->GAT N->D 349 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369157 AGT->GGT S->G 349 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369158 ACT->ATT T->I 335 T ARIGQTGTKSVFSQS 329 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369159 GCC->GTC A->V 312 S PPVIDVPSTGAPSKK 312 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369164 GTA->GAA V->E 312 S PPVIDVPSTGAPSKK 312 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208641 ACA->GCA T->A 370 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208642 AGT->AGG S->R 370 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208649 GAG->AAG E->K 349 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208650 AAT->AAG N->K 349 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208651 AGT->AGG S->R 349 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs35296055 GCA->ACA A->T 349 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3877713 CCT->GCT P->A 432 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3994102 CAA->CAC Q->H 432 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018095 CCC->CAC P->H 370 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018095 CCC->CAC P->H 372 T TPQVLSPTITSPPNA 366 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018096 GGC->AGC G->S 335 T ARIGQTGTKSVFSQS 329 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018103 TCC->TTC S->F 440 S LEITKLDSSIISEGK 434 Phosphorylation: PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4033963 CCA->CTA P->L 370 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4033963 CCA->CTA P->L 372 T TPQVLSPTITSPPNA 366 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs582550 AAT->GAT N->D 432 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs634140 TCT->TAT S->Y 370 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs700044 ACA->GCA T->A 370 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs711738 AAA->GAA K->E 452 T EGKISTITPQIQAFN 446 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs7340209 AAT->CAT N->H 209 T LTETPQDTIELNRLN 209 Phosphorylation: PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs7340209 AAT->CAT N->H 222 S LNLESSNSKYSLNTD 222 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs11491191 TCC->TTC S->F 434 S LEITKLDSSIISEGK 434 Phosphorylation: PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs1984700 ACA->ATA T->I 364 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs1984701 CCA->TCA P->S 366 T TPQVLSPTITSPPNA 366 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2311272 AAT->AGT N->S 446 T EGKISTITPQIQAFN 446 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2311273 GAC->TAC D->Y 434 S LEITKLDSSIISEGK 434 Phosphorylation: PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2311273 GAC->TAC D->Y 435 S EITKLDSSIISEGKI 435 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2311275 CAA->AAA Q->K 426 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2311280 TCA->CCA S->P 313 T PVIDVPSTGAPSKKS 313 Phosphorylation TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369155 CGA->CAA R->Q 343 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369156 AAT->GAT N->D 343 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369157 AGT->GGT S->G 343 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369158 ACT->ATT T->I 329 T ARIGQTGTKSVFSQS 329 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369159 GCC->GTC A->V 312 S PPVIDVPSTGAPSKK 312 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs2369164 GTA->GAA V->E 312 S PPVIDVPSTGAPSKK 312 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208641 ACA->GCA T->A 364 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208642 AGT->AGG S->R 364 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208649 GAG->AAG E->K 343 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208650 AAT->AAG N->K 343 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3208651 AGT->AGG S->R 343 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs35296055 GCA->ACA A->T 343 T SGNSREVTPILAQTQ 343 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs381939 CCA->ACA P->T 313 T PVIDVPSTGAPSKKS 313 Phosphorylation TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3877713 CCT->GCT P->A 426 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs3994102 CAA->CAC Q->H 426 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018082 GAC->AAC D->N 209 T LTETPQDTIELNRLN 209 Phosphorylation: PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018085 GTT->ATT V->I 205 T PETVLTETPQDTIEL 205 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018095 CCC->CAC P->H 364 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018095 CCC->CAC P->H 366 T TPQVLSPTITSPPNA 366 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018096 GGC->AGC G->S 329 T ARIGQTGTKSVFSQS 329 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4018103 TCC->TTC S->F 434 S LEITKLDSSIISEGK 434 Phosphorylation: PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4033963 CCA->CTA P->L 364 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs4033963 CCA->CTA P->L 366 T TPQVLSPTITSPPNA 366 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs582550 AAT->GAT N->D 426 S TQPNINDSLEITKLD 426 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs634140 TCT->TAT S->Y 364 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs700044 ACA->GCA T->A 364 S STTPQVLSPTITSPP 364 Phosphorylation: CDK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs711738 AAA->GAA K->E 446 T EGKISTITPQIQAFN 446 Phosphorylation: CDK1; PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs7340209 AAT->CAT N->H 209 T LTETPQDTIELNRLN 209 Phosphorylation: PLK1 TypeIII- CDC27; ANAPC3; D0S1430E; D17S978E rs7340209 AAT->CAT N->H 222 S LNLESSNSKYSLNTD 222 Phosphorylation: CDK1 TypeIII-

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P30260 K77-ac Acetylation QCKYLLAkCCVDLSK P30260 T68-p Phosphorylation LKGHSCTtPQCKYLL P30260 S154-p Phosphorylation FLWSPFEsLCEIGEK P30260 S177-p Phosphorylation FTSLQNFsNCLPNsC P30260 S183-p Phosphorylation FsNCLPNsCTtQVPN P30260 T186-p Phosphorylation CLPNsCTtQVPNHsL P30260 S192-p Phosphorylation TtQVPNHsLsHRQPE P30260 S194-p Phosphorylation QVPNHsLsHRQPEtV P30260 T200-p Phosphorylation LsHRQPEtVLtEtPQ P30260 T203-p Phosphorylation RQPEtVLtEtPQDtI P30260 T205-p Phosphorylation PEtVLtEtPQDtIEL P30260 T209-p Phosphorylation LtEtPQDtIELNRLN P30260 S219-p Phosphorylation LNRLNLEssNsKYSL P30260 S220-p Phosphorylation NRLNLEssNsKYSLN P30260 S222-p Phosphorylation LNLEssNsKYSLNTD P30260 S241-p Phosphorylation YIDSAVIsPDtVPLG P30260 T244-p Phosphorylation SAVIsPDtVPLGTGT P30260 S252-p Phosphorylation VPLGTGTsILSKQVQ P30260 T264-p Phosphorylation QVQNKPKtGRSLLGG P30260 S276-p Phosphorylation LGGPAALsPLtPSFG P30260 T279-p Phosphorylation PAALsPLtPSFGILP P30260 T289-p Phosphorylation FGILPLEtPsPGDGs P30260 S291-p Phosphorylation ILPLEtPsPGDGsYL P30260 S296-p Phosphorylation tPsPGDGsYLQNYTN P30260 T304-p Phosphorylation YLQNYTNtPPVIDVP P30260 S312-p Phosphorylation PPVIDVPstGAPSKk P30260 T313-p Phosphorylation PVIDVPstGAPSKkS P30260 T329-p Phosphorylation ARIGQTGtkSVFsQs P30260 S334-p Phosphorylation TGtkSVFsQsGNsRE P30260 S336-p Phosphorylation tkSVFsQsGNsREVt P30260 S339-p Phosphorylation VFsQsGNsREVtPIL P30260 T343-p Phosphorylation sGNsREVtPILAQTQ P30260 T359-p Phosphorylation SGPQTSTtPQVLsPt P30260 S364-p Phosphorylation STtPQVLsPtItsPP P30260 T366-p Phosphorylation tPQVLsPtItsPPNA P30260 T368-p Phosphorylation QVLsPtItsPPNALP P30260 S369-p Phosphorylation VLsPtItsPPNALPR P30260 S378-p Phosphorylation PNALPRRssRLFtsD P30260 S379-p Phosphorylation NALPRRssRLFtsDs P30260 T383-p Phosphorylation RRssRLFtsDssttK P30260 S384-p Phosphorylation RssRLFtsDssttKE P30260 S386-p Phosphorylation sRLFtsDssttKENs P30260 S387-p Phosphorylation RLFtsDssttKENsK P30260 T388-p Phosphorylation LFtsDssttKENsKK P30260 T389-p Phosphorylation FtsDssttKENsKKL P30260 S393-p Phosphorylation ssttKENsKKLKMKF P30260 S426-p Phosphorylation TQPNINDsLEItkLD P30260 T430-p Phosphorylation INDsLEItkLDssII P30260 S434-p Phosphorylation LEItkLDssIIsEGk P30260 S435-p Phosphorylation EItkLDssIIsEGkI P30260 S438-p Phosphorylation kLDssIIsEGkIStI P30260 T444-p Phosphorylation IsEGkIStItPQIQA P30260 T446-p Phosphorylation EGkIStItPQIQAFN P30260 S553-p Phosphorylation LQKDVALsVLSkDLT P30260 S578-p Phosphorylation CAAGNCFsLQREHDI P30260 T691-p Phosphorylation KSEkALDtLNkAIVI P30260 Y740-p Phosphorylation VPkESLVyFLIGKVY P30260 T809-p Phosphorylation ESQESSMtDADDTQL P30260 S821-p Phosphorylation TQLHAAEsDEF____ P30260 K77-ub Ubiquitination QCKYLLAkCCVDLSK P30260 K99-ub Ubiquitination LSGGVFNkQKSHDDI P30260 K133-ub Ubiquitination CKTDRLAkGSECYQK P30260 K319-ub Ubiquitination stGAPSKkSVARIGQ P30260 K330-ub Ubiquitination RIGQTGtkSVFsQsG P30260 K415-ub Ubiquitination KTKSKTNkGGITQPN P30260 K431-ub Ubiquitination NDsLEItkLDssIIs P30260 K441-ub Ubiquitination ssIIsEGkIStItPQ P30260 K557-ub Ubiquitination VALsVLSkDLTDMDK P30260 K588-ub Ubiquitination REHDIAIkFFQRAIQ P30260 K687-ub Ubiquitination HALKKSEkALDtLNk P30260 K694-ub Ubiquitination kALDtLNkAIVIDPk P30260 K701-ub Ubiquitination kAIVIDPkNPLCKFH P30260 K718-ub Ubiquitination SVLFANEkYkSALQE P30260 K720-ub Ubiquitination LFANEkYkSALQELE P30260 K730-ub Ubiquitination LQELEELkQIVPkES P30260 K735-ub Ubiquitination ELkQIVPkESLVyFL P30260 K776-ub Ubiquitination KGANNQIkEAIDKRY

PTM - dbPTM UniProt ID Position Code Type Resources References P30260 205 T Phosphorylation Swiss-Prot 1010711 14657031;18669648 P30260 209 T Phosphorylation Swiss-Prot 1010711 14657031 P30260 244 T Phosphorylation Swiss-Prot 1010711 14657031 P30260 291 S Phosphorylation Swiss-Prot 1010711 14657031 P30260 313 T Phosphorylation Swiss-Prot 1010711 14657031 P30260 339 S Phosphorylation Swiss-Prot 1010711 18669648;19007248 P30260 366 T Phosphorylation Swiss-Prot 1010711 19690332 P30260 426 S Phosphorylation Swiss-Prot 1010711 14657031;18669648;19413330 P30260 430 T Phosphorylation Swiss-Prot 1010711 14657031 P30260 435 S Phosphorylation Swiss-Prot 1010711 14657031;16565220;18669648;19007248 P30260 438 S Phosphorylation Swiss-Prot 1010711 16565220;18669648;19007248 P30260 446 T Phosphorylation Swiss-Prot 1010711 14657031;18669648;19413330 P30260 183 S Phosphorylation PhosphoELM.10011 22067460 P30260 220 S Phosphorylation PhosphoELM.10011 22067460;18669648 P30260 222 S Phosphorylation PhosphoELM.10011 22067460;14657031 P30260 241 S Phosphorylation PhosphoELM.10011 22067460 P30260 252 S Phosphorylation PhosphoELM.10011 22067460 P30260 276 S Phosphorylation PhosphoELM.10011 22067460 P30260 291 S Phosphorylation PhosphoELM.10011 14657031 P30260 296 S Phosphorylation PhosphoELM.10011 14657031 P30260 312 S Phosphorylation PhosphoELM.10011 22067460;14657031 P30260 320 S Phosphorylation PhosphoELM.10011 22067460 P30260 339 S Phosphorylation PhosphoELM.10011 22067460;18669648 P30260 369 S Phosphorylation PhosphoELM.10011 22067460 P30260 384 S Phosphorylation PhosphoELM.10011 18669648 P30260 386 S Phosphorylation PhosphoELM.10011 22067460;18669648 P30260 387 S Phosphorylation PhosphoELM.10011 22067460 P30260 393 S Phosphorylation PhosphoELM.10011 22067460 P30260 426 S Phosphorylation PhosphoELM.10011 22067460;18669648;14657031 P30260 435 S Phosphorylation PhosphoELM.10011 22067460;18669648;14657031 P30260 438 S Phosphorylation PhosphoELM.10011 22067460;18669648;16565220 P30260 364 S Phosphorylation PhosphoELM.10011 22067460;14657031 P30260 434 S Phosphorylation PhosphoELM.10011 18669648;14657031 P30260 244 T Phosphorylation PhosphoELM.10011 14657031 P30260 279 T Phosphorylation PhosphoELM.10011 22067460 P30260 304 T Phosphorylation PhosphoELM.10011 22067460 P30260 313 T Phosphorylation PhosphoELM.10011 22067460;14657031 P30260 329 T Phosphorylation PhosphoELM.10011 14657031 P30260 359 T Phosphorylation PhosphoELM.10011 22067460 P30260 366 T Phosphorylation PhosphoELM.10011 22067460;18669648;14657031 P30260 368 T Phosphorylation PhosphoELM.10011 22067460 P30260 388 T Phosphorylation PhosphoELM.10011 22067460 P30260 389 T Phosphorylation PhosphoELM.10011 22067460;18669648 P30260 205 T Phosphorylation PhosphoELM.10011 22067460;18669648;14657031 P30260 289 T Phosphorylation PhosphoELM.10011 14657031 P30260 343 T Phosphorylation PhosphoELM.10011 14657031 P30260 446 T Phosphorylation PhosphoELM.10011 22067460;18669648;14657031;14657031 P30260 209 T Phosphorylation PhosphoELM.10011 14657031 P30260 430 T Phosphorylation PhosphoELM.10011 18669648;14657031 P30260 330 K Ubiquitylation Phosphositeplus.1010730 21906983 P30260 415 K Ubiquitylation Phosphositeplus.1010730 21906983 P30260 431 K Ubiquitylation Phosphositeplus.1010730 21906983 P30260 441 K Ubiquitylation Phosphositeplus.1010730 21906983 P30260 557 K Ubiquitylation Phosphositeplus.1010730 21906983 P30260 588 K Ubiquitylation Phosphositeplus.1010730 21890473 P30260 694 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P30260 718 K Ubiquitylation Phosphositeplus.1010730 21906983 P30260 720 K Ubiquitylation Phosphositeplus.1010730 21906983 P30260 730 K Ubiquitylation Phosphositeplus.1010730 21906983 P30260 735 K Ubiquitylation Phosphositeplus.1010730 21890473 P30260 99 K Ubiquitylation Phosphositeplus.1010730 21906983 P30260 154 S Phosphorylation Phosphositeplus.1010730 21209074 P30260 183 S Phosphorylation Phosphositeplus.1010730 22067460 P30260 192 S Phosphorylation Phosphositeplus.1010730 22067460 P30260 220 S Phosphorylation Phosphositeplus.1010730 18420821;22067460 P30260 222 S Phosphorylation Phosphositeplus.1010730 14657031;22067460 P30260 241 S Phosphorylation Phosphositeplus.1010730 22067460 P30260 252 S Phosphorylation Phosphositeplus.1010730 22067460 P30260 276 S Phosphorylation Phosphositeplus.1010730 22067460 P30260 291 S Phosphorylation Phosphositeplus.1010730 14657031 P30260 296 S Phosphorylation Phosphositeplus.1010730 14657031 P30260 312 S Phosphorylation Phosphositeplus.1010730 14657031;22067460 P30260 334 S Phosphorylation Phosphositeplus.1010730 18420821 P30260 336 S Phosphorylation Phosphositeplus.1010730 18420821 P30260 339 S Phosphorylation Phosphositeplus.1010730 18669648;19691289;22067460 P30260 364 S Phosphorylation Phosphositeplus.1010730 19690332;22067460 P30260 369 S Phosphorylation Phosphositeplus.1010730 18420821;22067460 P30260 379 S Phosphorylation Phosphositeplus.1010730 22137581 P30260 384 S Phosphorylation Phosphositeplus.1010730 18420821;22067460 P30260 386 S Phosphorylation Phosphositeplus.1010730 18420821;18669648;20068231;22067460 P30260 387 S Phosphorylation Phosphositeplus.1010730 18420821;22067460 P30260 393 S Phosphorylation Phosphositeplus.1010730 22067460 P30260 426 S Phosphorylation Phosphositeplus.1010730 14657031;18420821;18669648;20068231;22067460;22115753 P30260 434 S Phosphorylation Phosphositeplus.1010730 14657031 P30260 435 S Phosphorylation Phosphositeplus.1010730 14657031;16565220;18420821;20068231;22067460 P30260 438 S Phosphorylation Phosphositeplus.1010730 16565220;18420821;18669648;19691289;20068231;22067460;22115753 P30260 553 S Phosphorylation Phosphositeplus.1010730 18420821 P30260 821 S Phosphorylation Phosphositeplus.1010730 22139841 P30260 203 T Phosphorylation Phosphositeplus.1010730 18420821 P30260 205 T Phosphorylation Phosphositeplus.1010730 14657031;18420821;18669648;20068231;22067460 P30260 209 T Phosphorylation Phosphositeplus.1010730 14657031;16964243 P30260 244 T Phosphorylation Phosphositeplus.1010730 14657031;20360007 P30260 264 T Phosphorylation Phosphositeplus.1010730 18420821 P30260 279 T Phosphorylation Phosphositeplus.1010730 22067460 P30260 289 T Phosphorylation Phosphositeplus.1010730 14657031 P30260 304 T Phosphorylation Phosphositeplus.1010730 22067460 P30260 313 T Phosphorylation Phosphositeplus.1010730 14657031 P30260 329 T Phosphorylation Phosphositeplus.1010730 14657031 P30260 343 T Phosphorylation Phosphositeplus.1010730 14657031 P30260 359 T Phosphorylation Phosphositeplus.1010730 22067460 P30260 366 T Phosphorylation Phosphositeplus.1010730 18420821;19276368;19690332;22067460 P30260 368 T Phosphorylation Phosphositeplus.1010730 22067460 P30260 383 T Phosphorylation Phosphositeplus.1010730 18420821 P30260 388 T Phosphorylation Phosphositeplus.1010730 22067460 P30260 389 T Phosphorylation Phosphositeplus.1010730 18420821;22067460 P30260 430 T Phosphorylation Phosphositeplus.1010730 14657031 P30260 444 T Phosphorylation Phosphositeplus.1010730 18420821 P30260 446 T Phosphorylation Phosphositeplus.1010730 14657031;16964243;18420821;18669648;20068231;22067460 P30260 691 T Phosphorylation Phosphositeplus.1010730 18420821 P30260 740 Y Phosphorylation Phosphositeplus.1010730 18180459 P30260 220 S Phosphorylation HPRD 9.0 18669648 P30260 336 S Phosphorylation HPRD 9.0 20068231 P30260 339 S Phosphorylation HPRD 9.0 18669648,19007248,19691289 P30260 364 S Phosphorylation HPRD 9.0 20068231 P30260 384 S Phosphorylation HPRD 9.0 18669648,20068231 P30260 386 S Phosphorylation HPRD 9.0 18669648,20068231 P30260 387 S Phosphorylation HPRD 9.0 20068231 P30260 426 S Phosphorylation HPRD 9.0 18669648,19413330,20068231 P30260 434 S Phosphorylation HPRD 9.0 18669648,20068231 P30260 435 S Phosphorylation HPRD 9.0 16565220,18669648,19007248,19651622,19691289,20068231 P30260 438 S Phosphorylation HPRD 9.0 16565220,18669648,19007248,19651622,19691289,20068231 P30260 205 T Phosphorylation HPRD 9.0 18669648,20068231 P30260 366 T Phosphorylation HPRD 9.0 18669648 P30260 388 T Phosphorylation HPRD 9.0 20068231 P30260 389 T Phosphorylation HPRD 9.0 18669648,20068231 P30260 430 T Phosphorylation HPRD 9.0 18669648 P30260 444 T Phosphorylation HPRD 9.0 20068231 P30260 446 T Phosphorylation HPRD 9.0 18669648,19413330,20068231 P30260 809 T Phosphorylation HPRD 9.0 18452278 P30260 339 S Phosphorylation SysPTM 1.1 18669648 P30260 426 S Phosphorylation SysPTM 1.1 18669648 P30260 435 S Phosphorylation SysPTM 1.1 18669648;16565220 P30260 438 S Phosphorylation SysPTM 1.1 18669648;16565220 P30260 205 T Phosphorylation SysPTM 1.1 18669648 P30260 209 T Phosphorylation SysPTM 1.1 16964243 P30260 446 T Phosphorylation SysPTM 1.1 18669648;16964243

PTM - HPRD Symbol Position Code Type References CDC27 205 T Phosphorylation 18669648,20068231 CDC27 220 S Phosphorylation 18669648 CDC27 336 S Phosphorylation 20068231 CDC27 339 S Phosphorylation 18669648,19007248,19691289 CDC27 364 S Phosphorylation 20068231 CDC27 366 T Phosphorylation 18669648 CDC27 384 S Phosphorylation 18669648,20068231 CDC27 386 S Phosphorylation 18669648,20068231 CDC27 387 S Phosphorylation 20068231 CDC27 388 T Phosphorylation 20068231 CDC27 389 T Phosphorylation 18669648,20068231 CDC27 426 S Phosphorylation 18669648,19413330,20068231 CDC27 430 T Phosphorylation 18669648 CDC27 434 S Phosphorylation 18669648,20068231 CDC27 435 S Phosphorylation 16565220,18669648,19007248,19651622,19691289,20068231 CDC27 438 S Phosphorylation 16565220,18669648,19007248,19651622,19691289,20068231 CDC27 444 T Phosphorylation 20068231 CDC27 446 T Phosphorylation 18669648,19413330,20068231 CDC27 809 T Phosphorylation 18452278

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P30260 222 S 22067460 HTP Phosphorylation P30260 222 S 14657031 HTP Phosphorylation P30260 220 S 22067460 HTP Phosphorylation P30260 220 S 18669648 HTP Phosphorylation P30260 343 T 14657031 CDK1 HTP Phosphorylation P30260 369 S 22067460 HTP Phosphorylation P30260 386 S 22067460 HTP Phosphorylation P30260 386 S 18669648 HTP Phosphorylation P30260 384 S 18669648 HTP Phosphorylation P30260 389 T 22067460 HTP Phosphorylation P30260 389 T 18669648 HTP Phosphorylation P30260 291 S 14657031 HTP Phosphorylation P30260 388 T 22067460 HTP Phosphorylation P30260 387 S 22067460 HTP Phosphorylation P30260 289 T 14657031 CDK1 HTP Phosphorylation P30260 393 S 22067460 HTP Phosphorylation P30260 279 T 22067460 HTP Phosphorylation P30260 276 S 22067460 HTP Phosphorylation P30260 426 S 22067460 HTP Phosphorylation P30260 426 S 18669648 HTP Phosphorylation P30260 426 S 14657031 HTP Phosphorylation P30260 339 S 22067460 HTP Phosphorylation P30260 339 S 18669648 HTP Phosphorylation P30260 438 S 22067460 HTP Phosphorylation P30260 438 S 18669648 HTP Phosphorylation P30260 438 S 16565220 HTP Phosphorylation P30260 296 S 14657031 HTP Phosphorylation P30260 435 S 22067460 HTP Phosphorylation P30260 435 S 18669648 HTP Phosphorylation P30260 435 S 14657031 HTP Phosphorylation P30260 430 T 18669648 HTP Phosphorylation P30260 430 T 14657031 PLK1 HTP Phosphorylation P30260 434 S 18669648 HTP Phosphorylation P30260 434 S 14657031 PLK1 HTP Phosphorylation P30260 304 T 22067460 HTP Phosphorylation P30260 313 T 22067460 HTP Phosphorylation P30260 313 T 14657031 HTP Phosphorylation P30260 209 T 14657031 PLK1 HTP Phosphorylation P30260 312 S 22067460 HTP Phosphorylation P30260 312 S 14657031 HTP Phosphorylation P30260 183 S 22067460 HTP Phosphorylation P30260 205 T 22067460 HTP Phosphorylation P30260 205 T 18669648 HTP Phosphorylation P30260 205 T 14657031 CDK1 HTP Phosphorylation P30260 446 T 22067460 HTP Phosphorylation P30260 446 T 18669648 HTP Phosphorylation P30260 446 T 14657031 CDK1 HTP Phosphorylation P30260 446 T 14657031 PLK1 HTP Phosphorylation P30260 329 T 14657031 HTP Phosphorylation P30260 320 S 22067460 HTP Phosphorylation P30260 368 T 22067460 HTP Phosphorylation P30260 366 T 22067460 HTP Phosphorylation P30260 366 T 18669648 HTP Phosphorylation P30260 366 T 14657031 HTP Phosphorylation P30260 364 S 22067460 HTP Phosphorylation P30260 364 S 14657031 CDK1 HTP Phosphorylation P30260 359 T 22067460 HTP Phosphorylation P30260 252 S 22067460 HTP Phosphorylation P30260 244 T 14657031 HTP Phosphorylation P30260 241 S 22067460 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P30260 205 Phosphothreonine 18669648;23186163;14657031 P30260 209 Phosphothreonine 14657031 P30260 244 Phosphothreonine 14657031 P30260 291 Phosphoserine 14657031 P30260 313 Phosphothreonine 14657031 P30260 339 Phosphoserine 18669648 P30260 366 Phosphothreonine 19690332 P30260 379 Phosphoserine 23186163 P30260 386 Phosphoserine 20068231 P30260 426 Phosphoserine 18669648;23186163;14657031 P30260 430 Phosphothreonine 14657031 P30260 435 Phosphoserine 14657031 P30260 438 Phosphoserine 18669648 P30260 446 Phosphothreonine 18669648;23186163;14657031 P30260 821 Phosphoserine 22139841

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type CDC27 IPI00294575 S 339 FSQSGNpSREVTPI phosphorylation CDC27 IPI00294575 S 384 SSRLFTpSDSSTTK phosphorylation CDC27 IPI00294575 S 386 RLFTSDpSSTTKEN phosphorylation CDC27 IPI00294575 S 387 LFTSDSpSTTKENS phosphorylation CDC27 IPI00294575 S 426 QPNINDpSLEITKL phosphorylation CDC27 IPI00294575 S 435 ITKLDSpSIISEGK phosphorylation CDC27 IPI00294575 T 205 ETVLTEpTPQDTIE phosphorylation CDC27 IPI00294575 T 383 RSSRLFpTSDSSTT phosphorylation CDC27 IPI00294575 T 388 FTSDSSpTTKENSK phosphorylation CDC27 IPI00294575 T 389 TSDSSTpTKENSKK phosphorylation CDC27 IPI00294575 T 444 SEGKISpTITPQIQ phosphorylation CDC27 IPI00294575 T 446 GKISTIpTPQIQAF phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 996 107431 - K Ubiquitination 21139048 996 107431 - K Ubiquitination 21890473 996 107431 - K Ubiquitination 21906983 996 107431 - K Ubiquitination 17187060 996 107431 - K Ubiquitination 21963094 996 107431 - K Ubiquitination 23000965 996 107431 - K Ubiquitination 25147182

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID CDC27 Homo sapiens (Human) 38; 99; 735; 694; 588 "38,SGGVFNKQKSHDD; 99,SGGVFNKQKSHDD; 735,LKQIVPKESLVYF; 694,ALDTLNKAIVIDP; 588,EHDIAIKFFQRAI" 38:21906983; 99:21890473; 735:21890473; 694:21890473; 588:21890473 CDC27 Homo sapiens (Human) 99 "99,SGGVFNKQKSHDD" 99:21890473 CDC27 Homo sapiens (Human) 441; 730; 99; 415; 431; 718; 735; 694; 720; 557; 330; 588 "441,SIISEGKISTITP; 730,QELEELKQIVPKE; 99,SGGVFNKQKSHDD; 415,TKSKTNKGGITQP; 431,DSLEITKLDSSII; 718,VLFANEKYKSALQ; 735,LKQIVPKESLVYF; 694,ALDTLNKAIVIDP; 720,FANEKYKSALQEL; 557,ALSVLSKDLTDMD; 330,IGQTGTKSVFSQS; 588,EHDIAIKFFQRAI" "441:21906983; 730:21906983; 99:21890473,21906983; 415:21906983; 431:21906983; 718:21906983; 735:21890473; 694:21890473,21906983; 720:21906983; 557:21906983; 330:21906983; 588:21890473" CDC27 Homo sapiens (Human) 441; 729; 717; 99; 415; 431; 719; 556; 693; 330 "441,SIISEGKISTITP; 729,QELEELKQIVPKE; 717,VLFANEKYKSALQ; 99,SGGVFNKQKSHDD; 415,TKSKTNKGGITQP; 431,DSLEITKLDSSII; 719,FANEKYKSALQEL; 556,ALSVLSKDLTDMD; 693,ALDTLNKAIVIDP; 330,IGQTGTKSVFSQS" 441:21906983; 729:21906983; 717:21906983; 99:21906983; 415:21906983; 431:21906983; 719:21906983; 556:21906983; 693:21906983; 330:21906983