PTM - CPLM Gene Name UniProt ID Type Position Code PIAS1; DDXBP1 O75925 Ubiquitination 8 K PIAS1; DDXBP1 O75925 Ubiquitination 56 K PIAS1; DDXBP1 O75925 Ubiquitination 46 K PIAS1; DDXBP1 O75925 Ubiquitination 315 K PIAS1; DDXBP1 O75925 Ubiquitination 453 K PIAS1; DDXBP1 O75925 Ubiquitination 455 K PIAS1; DDXBP1 O75925 Ubiquitination 456 K PIAS1; DDXBP1 O75925 Ubiquitination 58 K PIAS1; DDXBP1 O75925 Ubiquitination 140 K PIAS1; DDXBP1 O75925 Ubiquitination 419 K PIAS1; DDXBP1 O75925 Ubiquitination 493 K PIAS1; DDXBP1 O75925 Ubiquitination 226 K PIAS1; DDXBP1 O75925 Ubiquitination 238 K PIAS1; DDXBP1 O75925 Ubiquitination 339 K PIAS1; DDXBP1 O75925 Ubiquitination 408 K

PTM - dbPAF Gene Name UniProt ID Position Type Species PIAS1; DDXBP1 O75925 106 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 112 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 154 T Homo sapiens(Human) PIAS1; DDXBP1 O75925 158 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 289 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 290 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 291 T Homo sapiens(Human) PIAS1; DDXBP1 O75925 327 T Homo sapiens(Human) PIAS1; DDXBP1 O75925 328 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 451 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 463 T Homo sapiens(Human) PIAS1; DDXBP1 O75925 466 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 467 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 468 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 476 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 480 T Homo sapiens(Human) PIAS1; DDXBP1 O75925 483 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 485 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 487 T Homo sapiens(Human) PIAS1; DDXBP1 O75925 488 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 497 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 50 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 503 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 506 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 508 T Homo sapiens(Human) PIAS1; DDXBP1 O75925 510 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 522 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 633 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 634 S Homo sapiens(Human) PIAS1; DDXBP1 O75925 635 T Homo sapiens(Human) PIAS1; DDXBP1 O75925 71 T Homo sapiens(Human) PIAS1; DDXBP1 O75925 90 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA O75925 R303-m2 Methylation RLRAKGIrNPDHsRA O75925 S4-p Phosphorylation ____MADsAELkQMV O75925 S50-p Phosphorylation HLLkAGCsPAVQMkI O75925 T71-p Phosphorylation RFPQKIMtPADLSIP O75925 S254-p Phosphorylation SRPINITsLVRLSTT O75925 S289-p Phosphorylation VYLVKQLsstVLLQR O75925 S290-p Phosphorylation YLVKQLsstVLLQRL O75925 T291-p Phosphorylation LVKQLsstVLLQRLR O75925 S308-p Phosphorylation GIrNPDHsRALIKEk O75925 T327-p Phosphorylation PDSEIATtsLRVSLL O75925 S328-p Phosphorylation DSEIATtsLRVSLLC O75925 T463-p Phosphorylation KVEVIDLtIDsssDE O75925 S466-p Phosphorylation VIDLtIDsssDEEEE O75925 S467-p Phosphorylation IDLtIDsssDEEEEE O75925 S468-p Phosphorylation DLtIDsssDEEEEEP O75925 S476-p Phosphorylation DEEEEEPsAKRtCPs O75925 T480-p Phosphorylation EEPsAKRtCPsLsPt O75925 S483-p Phosphorylation sAKRtCPsLsPtsPL O75925 S485-p Phosphorylation KRtCPsLsPtsPLNN O75925 T487-p Phosphorylation tCPsLsPtsPLNNkG O75925 S488-p Phosphorylation CPsLsPtsPLNNkGI O75925 S497-p Phosphorylation LNNkGILsLPHQAsP O75925 S503-p Phosphorylation LsLPHQAsPVsRtPs O75925 S506-p Phosphorylation PHQAsPVsRtPsLPA O75925 T508-p Phosphorylation QAsPVsRtPsLPAVD O75925 S510-p Phosphorylation sPVsRtPsLPAVDTS O75925 S522-p Phosphorylation DTSYINTsLIQDYRH O75925 K8-sm Sumoylation MADsAELkQMVMSLR O75925 K40-sm Sumoylation RKHELLTkALHLLkA O75925 K46-sm Sumoylation TkALHLLkAGCsPAV O75925 K56-sm Sumoylation CsPAVQMkIkELYRR O75925 K58-sm Sumoylation PAVQMkIkELYRRRF O75925 K137-sm Sumoylation HPVHPDIkLQKLPFY O75925 K152-sm Sumoylation DLLDELIkPTSLASD O75925 K238-sm Sumoylation PGYLPPTkNGVEPKR O75925 K315-sm Sumoylation sRALIKEkLTADPDS O75925 K46-ub Ubiquitination TkALHLLkAGCsPAV O75925 K226-ub Ubiquitination LCVKVNTkPCSLPGY O75925 K315-ub Ubiquitination sRALIKEkLTADPDS O75925 K493-ub Ubiquitination PtsPLNNkGILsLPH

PTM - dbPTM UniProt ID Position Code Type Resources References O75925 303 R Methylation Swiss-Prot 1010711 19136629 O75925 466 S Phosphorylation Swiss-Prot 1010711 17081983 O75925 467 S Phosphorylation Swiss-Prot 1010711 17081983 O75925 468 S Phosphorylation Swiss-Prot 1010711 17081983 O75925 503 S Phosphorylation Swiss-Prot 1010711 18669648;19690332 O75925 510 S Phosphorylation Swiss-Prot 1010711 19690332 O75925 522 S Phosphorylation Swiss-Prot 1010711 18669648 O75925 466 S Phosphorylation PhosphoELM.10011 17081983 O75925 467 S Phosphorylation PhosphoELM.10011 17081983 O75925 468 S Phosphorylation PhosphoELM.10011 17081983 O75925 483 S Phosphorylation PhosphoELM.10011 17081983 O75925 485 S Phosphorylation PhosphoELM.10011 18669648 O75925 488 S Phosphorylation PhosphoELM.10011 18669648 O75925 503 S Phosphorylation PhosphoELM.10011 18669648 O75925 506 S Phosphorylation PhosphoELM.10011 18669648 O75925 522 S Phosphorylation PhosphoELM.10011 18669648 O75925 100 D Caspase Phosphositeplus.1010730 18722006 O75925 303 R Methylation Phosphositeplus.1010730 19136629 O75925 466 S Phosphorylation Phosphositeplus.1010730 19217413;20363803;21406692 O75925 467 S Phosphorylation Phosphositeplus.1010730 19217413;20363803;21406692 O75925 468 S Phosphorylation Phosphositeplus.1010730 19217413;20363803;21406692 O75925 476 S Phosphorylation Phosphositeplus.1010730 20363803 O75925 483 S Phosphorylation Phosphositeplus.1010730 20164059 O75925 485 S Phosphorylation Phosphositeplus.1010730 18669648;20164059 O75925 488 S Phosphorylation Phosphositeplus.1010730 21659604 O75925 503 S Phosphorylation Phosphositeplus.1010730 18669648;19690332;20068231;20363803;21659604;22115753 O75925 506 S Phosphorylation Phosphositeplus.1010730 20068231 O75925 510 S Phosphorylation Phosphositeplus.1010730 19690332 O75925 522 S Phosphorylation Phosphositeplus.1010730 18669648 O75925 508 T Phosphorylation Phosphositeplus.1010730 19690332 O75925 466 S Phosphorylation HPRD 9.0 17081983,19651622 O75925 467 S Phosphorylation HPRD 9.0 17081983,19651622 O75925 468 S Phosphorylation HPRD 9.0 17081983,19651622 O75925 485 S Phosphorylation HPRD 9.0 17081983,18669648 O75925 488 S Phosphorylation HPRD 9.0 17081983,18669648 O75925 503 S Phosphorylation HPRD 9.0 18669648,19651622,18452278,18767875,20068231 O75925 506 S Phosphorylation HPRD 9.0 18669648,20068231 O75925 522 S Phosphorylation HPRD 9.0 18669648 O75925 487 T Phosphorylation HPRD 9.0 17081983,20068231 O75925 635 T Phosphorylation HPRD 9.0 20068231 O75925 90 S Phosphorylation (CHUK) HPRD 9.0 17540171 O75925 466 S Phosphorylation SysPTM 1.1 17081983 O75925 467 S Phosphorylation SysPTM 1.1 17081983 O75925 468 S Phosphorylation SysPTM 1.1 17081983 O75925 485 S Phosphorylation SysPTM 1.1 17081983 O75925 488 S Phosphorylation SysPTM 1.1 17081983 O75925 503 S Phosphorylation SysPTM 1.1 18669648 O75925 522 S Phosphorylation SysPTM 1.1 18669648 O75925 487 T Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References PIAS1 466 S Phosphorylation 17081983,19651622 PIAS1 467 S Phosphorylation 17081983,19651622 PIAS1 468 S Phosphorylation 17081983,19651622 PIAS1 485 S Phosphorylation 17081983,18669648 PIAS1 487 T Phosphorylation 17081983,20068231 PIAS1 488 S Phosphorylation 17081983,18669648 PIAS1 503 S Phosphorylation 18669648,19651622,18452278,18767875,20068231 PIAS1 506 S Phosphorylation 18669648,20068231 PIAS1 522 S Phosphorylation 18669648 PIAS1 635 T Phosphorylation 20068231 PIAS1 90 S Phosphorylation 17540171

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type O75925 483 S 17081983 HTP Phosphorylation O75925 485 S 18669648 HTP Phosphorylation O75925 488 S 18669648 HTP Phosphorylation O75925 522 S 18669648 HTP Phosphorylation O75925 466 S 17081983 HTP Phosphorylation O75925 467 S 17081983 HTP Phosphorylation O75925 468 S 17081983 HTP Phosphorylation O75925 503 S 18669648 HTP Phosphorylation O75925 506 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs O75925 2 N-acetylalanine 22814378 O75925 303 Asymmetric dimethylarginine; by PRMT1 19136629 O75925 467 Phosphoserine 24275569 O75925 468 Phosphoserine 24275569 O75925 485 Phosphoserine 23186163 O75925 488 Phosphoserine 23186163 O75925 503 Phosphoserine 18669648;19690332;20068231;23186163;24275569 O75925 510 Phosphoserine 19690332 O75925 522 Phosphoserine 18669648

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type PIAS1 IPI00220424 S 466 IDLTIDpSSSDEEE phosphorylation PIAS1 IPI00220424 S 467 DLTIDSpSSDEEEE phosphorylation PIAS1 IPI00220424 S 468 LTIDSSpSDEEEEE phosphorylation PIAS1 IPI00220424 S 483 AKRTCPpSLSPTSP phosphorylation PIAS1 IPI00220424 S 503 SLPHQApSPVSRTP phosphorylation PIAS1 IPI00220424 S 506 HQASPVpSRTPSLP phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 8554 114124 - K Neddylation 18274552 8554 114124 - K Sumoylation 21693764 8554 114124 - K Ubiquitination 21139048 8554 114124 - K Ubiquitination 21906983 8554 114124 - K Sumoylation 19471022 8554 114124 - K Sumoylation 21252943 8554 114124 - K FAT10ylation 22797925 8554 114124 - K Ubiquitination 21963094 8554 114124 - K Ubiquitination 22505724 8554 114124 - K Sumoylation 24654937 8554 114124 - K Sumoylation 25391492

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID PIAS1 Homo sapiens (Human) 315 "315,RALIKEKLTADPD" 315:21906983