PTM - dbPAF Gene Name UniProt ID Position Type Species TNK2; ACK1 Q07912 101 T Homo sapiens(Human) TNK2; ACK1 Q07912 102 S Homo sapiens(Human) TNK2; ACK1 Q07912 136 S Homo sapiens(Human) TNK2; ACK1 Q07912 149 S Homo sapiens(Human) TNK2; ACK1 Q07912 193 Y Homo sapiens(Human) TNK2; ACK1 Q07912 205 T Homo sapiens(Human) TNK2; ACK1 Q07912 212 S Homo sapiens(Human) TNK2; ACK1 Q07912 284 Y Homo sapiens(Human) TNK2; ACK1 Q07912 36 S Homo sapiens(Human) TNK2; ACK1 Q07912 465 T Homo sapiens(Human) TNK2; ACK1 Q07912 517 T Homo sapiens(Human) TNK2; ACK1 Q07912 518 Y Homo sapiens(Human) TNK2; ACK1 Q07912 529 S Homo sapiens(Human) TNK2; ACK1 Q07912 603 T Homo sapiens(Human) TNK2; ACK1 Q07912 607 S Homo sapiens(Human) TNK2; ACK1 Q07912 635 Y Homo sapiens(Human) TNK2; ACK1 Q07912 724 S Homo sapiens(Human) TNK2; ACK1 Q07912 728 S Homo sapiens(Human) TNK2; ACK1 Q07912 730 S Homo sapiens(Human) TNK2; ACK1 Q07912 74 S Homo sapiens(Human) TNK2; ACK1 Q07912 774 S Homo sapiens(Human) TNK2; ACK1 Q07912 785 S Homo sapiens(Human) TNK2; ACK1 Q07912 789 S Homo sapiens(Human) TNK2; ACK1 Q07912 808 S Homo sapiens(Human) TNK2; ACK1 Q07912 810 S Homo sapiens(Human) TNK2; ACK1 Q07912 818 T Homo sapiens(Human) TNK2; ACK1 Q07912 827 Y Homo sapiens(Human) TNK2; ACK1 Q07912 829 T Homo sapiens(Human) TNK2; ACK1 Q07912 855 S Homo sapiens(Human) TNK2; ACK1 Q07912 856 S Homo sapiens(Human) TNK2; ACK1 Q07912 857 T Homo sapiens(Human) TNK2; ACK1 Q07912 859 Y Homo sapiens(Human) TNK2; ACK1 Q07912 860 Y Homo sapiens(Human) TNK2; ACK1 Q07912 867 S Homo sapiens(Human) TNK2; ACK1 Q07912 868 Y Homo sapiens(Human) TNK2; ACK1 Q07912 872 Y Homo sapiens(Human) TNK2; ACK1 Q07912 881 S Homo sapiens(Human) TNK2; ACK1 Q07912 925 T Homo sapiens(Human) TNK2; ACK1 Q07912 928 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q07912 K826-ac Acetylation QSFASDPkyAtPQVI Q07912 R839-m1 Methylation VIQAPGPrAGPCILP Q07912 R933-m1 Methylation NNSNPGArPPPPRAT Q07912 T33-p Phosphorylation LRDDLNVtRLsHFEy Q07912 S36-p Phosphorylation DLNVtRLsHFEyVKN Q07912 Y40-p Phosphorylation tRLsHFEyVKNEDLE Q07912 S74-p Phosphorylation KALCKRKsWMsKVFs Q07912 S77-p Phosphorylation CKRKsWMsKVFsGKR Q07912 S81-p Phosphorylation sWMsKVFsGKRLEAE Q07912 T101-p Phosphorylation SQSTFRKtsPAPGGP Q07912 S102-p Phosphorylation QSTFRKtsPAPGGPA Q07912 S136-p Phosphorylation LEkLGDGsFGVVRRG Q07912 S149-p Phosphorylation RGEWDAPsGKTVSVA Q07912 Y193-p Phosphorylation HRNLIRLyGVVLTPP Q07912 T205-p Phosphorylation TPPMKMVtELAPLGs Q07912 S212-p Phosphorylation tELAPLGsLLDRLRK Q07912 Y284-p Phosphorylation LPQNDDHyVMQEHRK Q07912 T465-p Phosphorylation LQNSFIHtGHGDSDP Q07912 T517-p Phosphorylation LGGVKKPtyDPVSED Q07912 Y518-p Phosphorylation GGVKKPtyDPVSEDQ Q07912 T603-p Phosphorylation ACSLLDEtPPQsPTR Q07912 S607-p Phosphorylation LDEtPPQsPTRALPR Q07912 Y635-p Phosphorylation PLPPPPAyDDVAQDE Q07912 S724-p Phosphorylation QLQAPAGsPAPsPsP Q07912 S728-p Phosphorylation PAGsPAPsPsPGGDD Q07912 S730-p Phosphorylation GsPAPsPsPGGDDKP Q07912 S774-p Phosphorylation SQWPGPAsPPRVPPR Q07912 S785-p Phosphorylation VPPREPLsPQGsRTP Q07912 S789-p Phosphorylation EPLsPQGsRTPSPLV Q07912 S810-p Phosphorylation LPPRLSSsPGKTMPT Q07912 T818-p Phosphorylation PGKTMPTtQSFASDP Q07912 Y827-p Phosphorylation SFASDPkyAtPQVIQ Q07912 T829-p Phosphorylation ASDPkyAtPQVIQAP Q07912 S855-p Phosphorylation VRDGKKVsstHyyLL Q07912 S856-p Phosphorylation RDGKKVsstHyyLLP Q07912 T857-p Phosphorylation DGKKVsstHyyLLPE Q07912 Y859-p Phosphorylation KKVsstHyyLLPERP Q07912 Y860-p Phosphorylation KVsstHyyLLPERPs Q07912 S867-p Phosphorylation yLLPERPsyLERyQR Q07912 Y868-p Phosphorylation LLPERPsyLERyQRF Q07912 Y872-p Phosphorylation RPsyLERyQRFLREA Q07912 S881-p Phosphorylation RFLREAQsPEEPTPL Q07912 T925-p Phosphorylation DPKANFStNNSNPGA Q07912 K131-ub Ubiquitination KDLRLLEkLGDGsFG

PTM - dbPTM UniProt ID Position Code Type Resources References Q07912 101 T Phosphorylation Swiss-Prot 1010711 18691976;19369195 Q07912 149 S Phosphorylation Swiss-Prot 1010711 17081983 Q07912 284 Y Phosphorylation Swiss-Prot 1010711 15308621;16472662;17389395;19369195;20333297;20623637;20979614;21169560;21309750 Q07912 517 T Phosphorylation Swiss-Prot 1010711 19369195 Q07912 518 Y Phosphorylation Swiss-Prot 1010711 15592455;17389395;18083107;19369195;21169560 Q07912 724 S Phosphorylation Swiss-Prot 1010711 19369195 Q07912 728 S Phosphorylation Swiss-Prot 1010711 19369195 Q07912 785 S Phosphorylation Swiss-Prot 1010711 18691976;19369195 Q07912 827 Y Phosphorylation Swiss-Prot 1010711 12522270;17081983;19369195;21169560 Q07912 859 Y Phosphorylation Swiss-Prot 1010711 12522270;15951569;17389395;18083107;21169560 Q07912 860 Y Phosphorylation Swiss-Prot 1010711 12522270;15951569;17389395;19534553 Q07912 872 Y Phosphorylation Swiss-Prot 1010711 21169560 Q07912 881 S Phosphorylation Swiss-Prot 1010711 18691976 Q07912 925 T Phosphorylation Swiss-Prot 1010711 19369195 Q07912 102 S Phosphorylation PhosphoELM.10011 18669648 Q07912 518 Y Phosphorylation PhosphoELM.10011 18083107;15592455 Q07912 827 Y Phosphorylation PhosphoELM.10011 17081983;12522270 Q07912 859 Y Phosphorylation PhosphoELM.10011 12522270 Q07912 860 Y Phosphorylation PhosphoELM.10011 12522270 Q07912 284 Y Phosphorylation PhosphoELM.10011 16472662;14506255 Q07912 149 S Phosphorylation Phosphositeplus.1010730 17081983 Q07912 724 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;19369195 Q07912 728 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;19369195 Q07912 730 S Phosphorylation Phosphositeplus.1010730 18669648;18691976 Q07912 785 S Phosphorylation Phosphositeplus.1010730 18691976;19369195 Q07912 856 S Phosphorylation Phosphositeplus.1010730 22322096 Q07912 867 S Phosphorylation Phosphositeplus.1010730 22322096 Q07912 881 S Phosphorylation Phosphositeplus.1010730 18691976 Q07912 101 T Phosphorylation Phosphositeplus.1010730 18691976 Q07912 857 T Phosphorylation Phosphositeplus.1010730 22322096 Q07912 925 T Phosphorylation Phosphositeplus.1010730 19369195 Q07912 284 Y Phosphorylation Phosphositeplus.1010730 14506255;16472662;17016520;17389395;18083107;19369195;20007894;20090780;20190765;20333297;20383201;20623637;21253578 Q07912 518 Y Phosphorylation Phosphositeplus.1010730 15592455;16497976;17016520;17360941;17389395;17646646;18083107;18180459;19369195;19658100;19901323;20007894;20090780;20190765;20562096;20736484;21049007;21394647 Q07912 635 Y Phosphorylation Phosphositeplus.1010730 20736484 Q07912 827 Y Phosphorylation Phosphositeplus.1010730 12522270;17870612;18691976;18707149;19369195;20736484 Q07912 859 Y Phosphorylation Phosphositeplus.1010730 12522270;15951569;17016520;17360941;17389395;18083107;18180459;18271526;19534553;19901323;20007894;20090780;20190765;20736484;21049007;22322096 Q07912 860 Y Phosphorylation Phosphositeplus.1010730 12522270;15951569;17016520;17360941;17389395;18083107;18180459;18271526;19534553;20090780;20190765;20736484;22322096 Q07912 868 Y Phosphorylation Phosphositeplus.1010730 18083107;22322096 Q07912 872 Y Phosphorylation Phosphositeplus.1010730 18083107;20736484 Q07912 102 S Phosphorylation HPRD 9.0 18669648 Q07912 724 S Phosphorylation HPRD 9.0 18669648,18691976 Q07912 728 S Phosphorylation HPRD 9.0 18669648,18691976 Q07912 730 S Phosphorylation HPRD 9.0 18669648,18691976 Q07912 785 S Phosphorylation HPRD 9.0 18691976 Q07912 808 S Phosphorylation HPRD 9.0 18669648,18691976 Q07912 881 S Phosphorylation HPRD 9.0 18691976 Q07912 101 T Phosphorylation HPRD 9.0 18691976 Q07912 517 T Phosphorylation HPRD 9.0 18691976 Q07912 284 Y Phosphorylation HPRD 9.0 14506255,17389395 Q07912 518 Y Phosphorylation HPRD 9.0 15592455,17389395,16497976,19901323 Q07912 827 Y Phosphorylation HPRD 9.0 14739304,18691976,18707149 Q07912 859 Y Phosphorylation HPRD 9.0 15951569,17389395,19901323 Q07912 860 Y Phosphorylation HPRD 9.0 15951569,17389395 Q07912 284 Y Phosphorylation (FYN) HPRD 9.0 14506255,17389395 Q07912 149 S Phosphorylation SysPTM 1.1 17081983 Q07912 808 S Phosphorylation SysPTM 1.1 18669648 Q07912 724 S Phosphorylation SysPTM 1.1 18669648 Q07912 728 S Phosphorylation SysPTM 1.1 18669648 Q07912 785 S Phosphorylation SysPTM 1.1 18691976 Q07912 881 S Phosphorylation SysPTM 1.1 18691976 Q07912 101 T Phosphorylation SysPTM 1.1 18691976 Q07912 827 Y Phosphorylation SysPTM 1.1 18707149

PTM - HPRD Symbol Position Code Type References TNK2 101 T Phosphorylation 18691976 TNK2 102 S Phosphorylation 18669648 TNK2 164 T Phosphorylation 18691976 TNK2 165 S Phosphorylation 18669648 TNK2 283 Y Phosphorylation 17016520 TNK2 284 Y Phosphorylation 14506255,17389395 TNK2 346 Y Phosphorylation 17016520 TNK2 347 Y Phosphorylation 14506255,17389395 TNK2 517 T Phosphorylation 18691976 TNK2 517 Y Phosphorylation 17016520 TNK2 518 Y Phosphorylation 15592455,17389395,16497976,19901323 TNK2 595 T Phosphorylation 18691976 TNK2 595 Y Phosphorylation 17016520 TNK2 596 Y Phosphorylation 15592455,17389395,16497976,19901323 TNK2 724 S Phosphorylation 18669648,18691976 TNK2 728 S Phosphorylation 18669648,18691976 TNK2 730 S Phosphorylation 18669648,18691976 TNK2 785 S Phosphorylation 18691976 TNK2 802 S Phosphorylation 18669648,18691976 TNK2 806 S Phosphorylation 18669648,18691976 TNK2 808 S Phosphorylation 18669648,18691976 TNK2 827 Y Phosphorylation 14739304,18691976,18707149 TNK2 858 Y Phosphorylation 17016520 TNK2 859 Y Phosphorylation 15951569,17389395,19901323 TNK2 860 Y Phosphorylation 15951569,17389395 TNK2 863 S Phosphorylation 18691976 TNK2 881 S Phosphorylation 18691976 TNK2 905 Y Phosphorylation 14739304,18691976,18707149 TNK2 936 Y Phosphorylation 17016520 TNK2 937 Y Phosphorylation 15951569,17389395,19901323 TNK2 938 Y Phosphorylation 15951569,17389395 TNK2 959 S Phosphorylation 18691976

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q07912 284 Y 16472662 TNK2 LTP Phosphorylation Q07912 284 Y 14506255 TNK2 LTP Phosphorylation Q07912 518 Y 18083107 HTP Phosphorylation Q07912 518 Y 15592455 HTP Phosphorylation Q07912 102 S 18669648 HTP Phosphorylation Q07912 857 Y 12522270 HTP Phosphorylation Q07912 858 Y 12522270 HTP Phosphorylation Q07912 826 Y 17081983 HTP Phosphorylation Q07912 826 Y 12522270 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q07912 518 Phosphotyrosine 21169560 Q07912 724 Phosphoserine 19369195 Q07912 827 Phosphotyrosine 21169560 Q07912 859 Phosphotyrosine 21169560 Q07912 872 Phosphotyrosine 21169560 Q07912 881 Phosphoserine 18691976

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type TNK2 IPI00442025 S 212 GEWDAPpSGKTVSV phosphorylation TNK2 IPI00442025 S 802 LQAPAGpSPAPSPS phosphorylation TNK2 IPI00442025 S 806 AGSPAPpSPSPGGD phosphorylation TNK2 IPI00442025 S 808 SPAPSPpSPGGDDK phosphorylation TNK2 IPI00442025 S 863 PPREPLpSPQGSRT phosphorylation TNK2 IPI00442025 S 959 FLREAQpSPEEPTP phosphorylation TNK2 IPI00442025 T 1003 PKANFSpTNNSNPG phosphorylation TNK2 IPI00442025 T 164 QSTFRKpTSPAPGG phosphorylation TNK2 IPI00442025 Y 347 PQNDDHpYVMQEHR phosphorylation TNK2 IPI00442025 Y 596 FTQKPTpYDPVSED phosphorylation TNK2 IPI00442025 Y 905 FASDPKpYATPQVI phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 10188 115485 - K Ubiquitination 20086093 10188 115485 - K Ubiquitination 19144635 10188 115485 - K Ubiquitination 21139048 10188 115485 - K Ubiquitination 23208506 10188 115485 - K Ubiquitination 21963094 10188 115485 - K Ubiquitination 23000965 10188 115485 - K Ubiquitination 24413169 10188 115485 - K Ubiquitination 25015289