PTM - CPLM Gene Name UniProt ID Type Position Code EPN1 Q9Y6I3 Ubiquitination 107 K EPN1 Q9Y6I3 Ubiquitination 11 K EPN1 Q9Y6I3 Ubiquitination 23 K EPN1 Q9Y6I3 Ubiquitination 86 K EPN1 Q9Y6I3 Ubiquitination 97 K EPN1 Q9Y6I3 Ubiquitination 117 K EPN1 Q9Y6I3 Ubiquitination 128 K EPN1 Q9Y6I3 Ubiquitination 469 K EPN1 Q9Y6I3 Ubiquitination 246 K EPN1 Q9Y6I3 Ubiquitination 149 K

PTM - dbPAF Gene Name UniProt ID Position Type Species EPN1 Q9Y6I3 101 Y Homo sapiens(Human) EPN1 Q9Y6I3 111 Y Homo sapiens(Human) EPN1 Q9Y6I3 17 Y Homo sapiens(Human) EPN1 Q9Y6I3 18 S Homo sapiens(Human) EPN1 Q9Y6I3 204 S Homo sapiens(Human) EPN1 Q9Y6I3 218 S Homo sapiens(Human) EPN1 Q9Y6I3 220 S Homo sapiens(Human) EPN1 Q9Y6I3 382 S Homo sapiens(Human) EPN1 Q9Y6I3 416 T Homo sapiens(Human) EPN1 Q9Y6I3 417 S Homo sapiens(Human) EPN1 Q9Y6I3 419 S Homo sapiens(Human) EPN1 Q9Y6I3 420 S Homo sapiens(Human) EPN1 Q9Y6I3 435 S Homo sapiens(Human) EPN1 Q9Y6I3 442 S Homo sapiens(Human) EPN1 Q9Y6I3 447 S Homo sapiens(Human) EPN1 Q9Y6I3 454 S Homo sapiens(Human) EPN1 Q9Y6I3 460 T Homo sapiens(Human) EPN1 Q9Y6I3 462 T Homo sapiens(Human) EPN1 Q9Y6I3 464 T Homo sapiens(Human) EPN1 Q9Y6I3 467 T Homo sapiens(Human) EPN1 Q9Y6I3 470 T Homo sapiens(Human) EPN1 Q9Y6I3 473 S Homo sapiens(Human) EPN1 Q9Y6I3 486 S Homo sapiens(Human) EPN1 Q9Y6I3 489 S Homo sapiens(Human) EPN1 Q9Y6I3 494 T Homo sapiens(Human) EPN1 Q9Y6I3 501 S Homo sapiens(Human) EPN1 Q9Y6I3 536 S Homo sapiens(Human) EPN1 Q9Y6I3 547 T Homo sapiens(Human) EPN1 Q9Y6I3 548 Y Homo sapiens(Human) EPN1 Q9Y6I3 550 S Homo sapiens(Human)

PTM - PhosSNP Gene Name dbSNP ID Gene Mutation AA Mutation Mutation Position Code Peptide Score Modification Type EPN1 rs61736521 CCG->CTG P->L 468 T LVSRPGPTPPGAKAS 469 Phosphorylation TypeIII-

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9Y6I3 Y17-p Phosphorylation MkNIVHNysEAEIkV Q9Y6I3 S18-p Phosphorylation kNIVHNysEAEIkVR Q9Y6I3 Y101-p Phosphorylation QQCkENMyAVQTLkD Q9Y6I3 Y111-p Phosphorylation QTLkDFQyVDRDGkD Q9Y6I3 S204-p Phosphorylation EEADQPPsCGPEDDA Q9Y6I3 S382-p Phosphorylation FSDPWGGsPAKPSTN Q9Y6I3 T412-p Phosphorylation SDFDRLRtALPtsGs Q9Y6I3 T416-p Phosphorylation RLRtALPtsGssAGE Q9Y6I3 S417-p Phosphorylation LRtALPtsGssAGEL Q9Y6I3 S419-p Phosphorylation tALPtsGssAGELEL Q9Y6I3 S420-p Phosphorylation ALPtsGssAGELELL Q9Y6I3 S435-p Phosphorylation AGEVPARsPGAFDMs Q9Y6I3 S442-p Phosphorylation sPGAFDMsGVRGsLA Q9Y6I3 S447-p Phosphorylation DMsGVRGsLAEAVGs Q9Y6I3 S454-p Phosphorylation sLAEAVGsPPPAAtP Q9Y6I3 T460-p Phosphorylation GsPPPAAtPtPtPPt Q9Y6I3 T462-p Phosphorylation PPPAAtPtPtPPtRk Q9Y6I3 T464-p Phosphorylation PAAtPtPtPPtRktP Q9Y6I3 T467-p Phosphorylation tPtPtPPtRktPEsF Q9Y6I3 T470-p Phosphorylation PtPPtRktPEsFLGP Q9Y6I3 S473-p Phosphorylation PtRktPEsFLGPNAA Q9Y6I3 S486-p Phosphorylation AALVDLDsLVSRPGP Q9Y6I3 T494-p Phosphorylation LVSRPGPtPPGAKAs Q9Y6I3 S501-p Phosphorylation tPPGAKAsNPFLPGG Q9Y6I3 S536-p Phosphorylation TLNQLRLsPVPPVPG Q9Y6I3 Y548-p Phosphorylation VPGAPPTyISPLGGG Q9Y6I3 K11-ub Ubiquitination SSLRRQMkNIVHNys Q9Y6I3 K23-ub Ubiquitination NysEAEIkVREATSN Q9Y6I3 K86-ub Ubiquitination TLMEYLIkTGSERVS Q9Y6I3 K97-ub Ubiquitination ERVSQQCkENMyAVQ Q9Y6I3 K107-ub Ubiquitination MyAVQTLkDFQyVDR Q9Y6I3 K117-ub Ubiquitination QyVDRDGkDQGVNVR Q9Y6I3 K128-ub Ubiquitination VNVREKAkQLVALLR Q9Y6I3 K149-ub Ubiquitination EERAHALkTKEKLAQ Q9Y6I3 K246-ub Ubiquitination QMAIEESkRETGGKE Q9Y6I3 K469-ub Ubiquitination tPtPPtRktPEsFLG

PTM - dbPTM UniProt ID Position Code Type Resources References Q9Y6I3 382 S Phosphorylation Swiss-Prot 1010711 10764745 Q9Y6I3 419 S Phosphorylation Swiss-Prot 1010711 19690332 Q9Y6I3 420 S Phosphorylation Swiss-Prot 1010711 19690332 Q9Y6I3 435 S Phosphorylation Swiss-Prot 1010711 18669648;19690332 Q9Y6I3 454 S Phosphorylation Swiss-Prot 1010711 18669648;19413330;19690332 Q9Y6I3 460 T Phosphorylation Swiss-Prot 1010711 18669648;19690332 Q9Y6I3 464 T Phosphorylation Swiss-Prot 1010711 18669648 Q9Y6I3 470 T Phosphorylation Swiss-Prot 1010711 19690332 Q9Y6I3 494 T Phosphorylation Swiss-Prot 1010711 17081983;17924679;18669648 Q9Y6I3 417 S Phosphorylation PhosphoELM.10011 18669648 Q9Y6I3 435 S Phosphorylation PhosphoELM.10011 18669648 Q9Y6I3 454 S Phosphorylation PhosphoELM.10011 18669648 Q9Y6I3 382 S Phosphorylation PhosphoELM.10011 10764745 Q9Y6I3 460 T Phosphorylation PhosphoELM.10011 18669648 Q9Y6I3 462 T Phosphorylation PhosphoELM.10011 18669648 Q9Y6I3 464 T Phosphorylation PhosphoELM.10011 18669648 Q9Y6I3 494 T Phosphorylation PhosphoELM.10011 18669648;17081983 Q9Y6I3 107 K Ubiquitylation Phosphositeplus.1010730 20639865;21890473;21906983 Q9Y6I3 11 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9Y6I3 117 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9Y6I3 128 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9Y6I3 149 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9Y6I3 23 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9Y6I3 246 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9Y6I3 469 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9Y6I3 86 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9Y6I3 97 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9Y6I3 382 S Phosphorylation Phosphositeplus.1010730 10764745 Q9Y6I3 419 S Phosphorylation Phosphositeplus.1010730 19690332;20166139;20562096 Q9Y6I3 420 S Phosphorylation Phosphositeplus.1010730 19690332;20166139;21659604 Q9Y6I3 435 S Phosphorylation Phosphositeplus.1010730 18669648;18707149;19276368;19690332;20068231;22115753 Q9Y6I3 447 S Phosphorylation Phosphositeplus.1010730 18491316 Q9Y6I3 454 S Phosphorylation Phosphositeplus.1010730 16964243;18669648;19690332;20068231;20363803;22115753 Q9Y6I3 416 T Phosphorylation Phosphositeplus.1010730 20562096 Q9Y6I3 460 T Phosphorylation Phosphositeplus.1010730 16964243;18669648;19690332;20068231;22115753 Q9Y6I3 464 T Phosphorylation Phosphositeplus.1010730 16964243;18669648;20068231 Q9Y6I3 470 T Phosphorylation Phosphositeplus.1010730 19367720;19690332;20068231;20363803;22115753 Q9Y6I3 494 T Phosphorylation Phosphositeplus.1010730 18669648;20068231;20363803;22115753 Q9Y6I3 111 Y Phosphorylation Phosphositeplus.1010730 18083107 Q9Y6I3 17 Y Phosphorylation Phosphositeplus.1010730 17016520;19534553;20007894 Q9Y6I3 435 S Phosphorylation SysPTM 1.1 18707149;18669648 Q9Y6I3 447 S Phosphorylation SysPTM 1.1 18491316 Q9Y6I3 454 S Phosphorylation SysPTM 1.1 16964243 Q9Y6I3 454 S Phosphorylation SysPTM 1.1 16964243;18669648 Q9Y6I3 460 T Phosphorylation SysPTM 1.1 16964243 Q9Y6I3 460 T Phosphorylation SysPTM 1.1 18669648;16964243 Q9Y6I3 464 T Phosphorylation SysPTM 1.1 16964243 Q9Y6I3 464 T Phosphorylation SysPTM 1.1 16964243;18669648 Q9Y6I3 494 T Phosphorylation SysPTM 1.1 18669648;17081983

PTM - HPRD Symbol Position Code Type References EPN1 16 Y Phosphorylation 17016520 EPN1 357 S Phosphorylation 10764745 EPN1 393 S Phosphorylation 20166139 EPN1 394 S Phosphorylation 20166139 EPN1 409 S Phosphorylation 18669648,20068231 EPN1 416 S Phosphorylation 18669648 EPN1 421 S Phosphorylation 18491316 EPN1 428 S Phosphorylation 18669648,16964243,20068231 EPN1 434 T Phosphorylation 18669648,16964243,20068231 EPN1 436 T Phosphorylation 18669648,20068231 EPN1 438 T Phosphorylation 18669648,20068231 EPN1 444 T Phosphorylation 20068231 EPN1 460 S Phosphorylation 20068231 EPN1 468 T Phosphorylation 17081983,18669648,17924679,20068231 EPN1 475 S Phosphorylation 20068231 EPN1 510 S Phosphorylation 20068231 EPN1 521 T Phosphorylation 20068231 EPN1 524 S Phosphorylation 20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9Y6I3 417 S 18669648 HTP Phosphorylation Q9Y6I3 357 S 10764745 CDK1 LTP Phosphorylation Q9Y6I3 429 S 18669648 HTP Phosphorylation Q9Y6I3 435 T 18669648 HTP Phosphorylation Q9Y6I3 437 T 18669648 HTP Phosphorylation Q9Y6I3 439 T 18669648 HTP Phosphorylation Q9Y6I3 410 S 18669648 HTP Phosphorylation Q9Y6I3 469 T 18669648 HTP Phosphorylation Q9Y6I3 469 T 17081983 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9Y6I3 382 Phosphoserine; by CDK1 10764745 Q9Y6I3 419 Phosphoserine 19690332;24275569 Q9Y6I3 420 Phosphoserine 19690332;24275569 Q9Y6I3 435 Phosphoserine 18669648;19690332;20068231;23186163;24275569 Q9Y6I3 447 Phosphoserine 23186163;24275569 Q9Y6I3 454 Phosphoserine 16964243;18669648;19690332;20068231;23186163;24275569 Q9Y6I3 460 Phosphothreonine 16964243;18669648;19690332;20068231 Q9Y6I3 464 Phosphothreonine 24275569 Q9Y6I3 470 Phosphothreonine 19690332 Q9Y6I3 494 Phosphothreonine 17081983;18669648;20068231

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type EPN1 IPI00002495 S 410 GEVPARpSPGAFDM phosphorylation EPN1 IPI00002495 S 429 LAEAVGpSPPPAAT phosphorylation EPN1 IPI00002495 S 476 PPGAKApSNPFLPG phosphorylation EPN1 IPI00002495 T 435 SPPPAApTPTPTPP phosphorylation EPN1 IPI00002495 T 437 PPAATPpTPTPPTR phosphorylation EPN1 IPI00002495 T 439 AATPTPpTPPTRKT phosphorylation EPN1 IPI00002495 T 445 TPPTRKpTPESFLG phosphorylation EPN1 IPI00002495 T 469 VSRPGPpTPPGAKA phosphorylation EPN1 IPI00027082 T 494 VSRPGPpTPPGAKA phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 29924 118965 - K Ubiquitination 16574660 29924 118965 - K Ubiquitination 20639865 29924 118965 - K Ubiquitination 21987572 29924 118965 - K Ubiquitination 21963094 29924 118965 - K Ubiquitination 21139048 29924 118965 - K Ubiquitination 21890473 29924 118965 - K Ubiquitination 21906983 29924 118965 - K Ubiquitination 15809337 29924 118965 - K Ubiquitination 22053931 29924 118965 - K Ubiquitination 23000965 29924 118965 - K Ubiquitination 24816145 29924 118965 - K Ubiquitination 25015289 29924 118965 - K Ubiquitination 25147182

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID EPN1 Homo sapiens (Human) 107 "107,YAVQTLKDFQYVD" "107:21139048,21906983,22053931" EPN1 Homo sapiens (Human) 117; 86; 443; 23; 97; 128; 11; 107 "117,YVDRDGKDQGVNV; 86,LMEYLIKTGSERV; 443,PTPPTRKTPESFL; 23,YSEAEIKVREATS; 97,RVSQQCKENMYAV; 128,NVREKAKQLVALL; 11,SLRRQMKNIVHNY; 107,YAVQTLKDFQYVD" 117:21890473; 86:21890473; 443:21890473; 23:21890473; 97:21890473; 128:21890473; 11:21890473; 107:21890473 EPN1 Homo sapiens (Human) 221; 117; 86; 443; 23; 97; 128; 11; 107; 149 "221,MAIEESKRETGGK; 117,YVDRDGKDQGVNV; 86,LMEYLIKTGSERV; 443,PTPPTRKTPESFL; 23,YSEAEIKVREATS; 97,RVSQQCKENMYAV; 128,NVREKAKQLVALL; 11,SLRRQMKNIVHNY; 107,YAVQTLKDFQYVD; 149,ERAHALKTKEKLA" "221:21906983; 117:21890473,21906983; 86:21890473,21906983; 443:21890473; 23:21890473,21906983; 97:21890473; 128:21890473; 11:21890473,21906983; 107:21890473,21906983; 149:21906983" EPN1 Homo sapiens (Human) 117; 86; 23; 97; 246; 128; 11; 469; 107; 149 "117,YVDRDGKDQGVNV; 86,LMEYLIKTGSERV; 23,YSEAEIKVREATS; 97,RVSQQCKENMYAV; 246,MAIEESKRETGGK; 128,NVREKAKQLVALL; 11,SLRRQMKNIVHNY; 469,PTPPTRKTPESFL; 107,YAVQTLKDFQYVD; 149,ERAHALKTKEKLA" "117:21890473,21906983; 86:21890473,21906983; 23:21890473,21906983; 97:21890473; 246:21906983; 128:21890473; 11:21890473,21906983; 469:21890473; 107:21890473,21906983; 149:21906983" EPN1 Homo sapiens (Human) 221; 117; 86; 444; 23; 97; 128; 11; 107; 149 "221,MAIEESKRETGGK; 117,YVDRDGKDQGVNV; 86,LMEYLIKTGSERV; 444,PTPPTRKTPESFL; 23,YSEAEIKVREATS; 97,RVSQQCKENMYAV; 128,NVREKAKQLVALL; 11,SLRRQMKNIVHNY; 107,YAVQTLKDFQYVD; 149,ERAHALKTKEKLA" "221:21906983; 117:21890473,21906983; 86:21890473,21906983; 444:21890473; 23:21890473,21906983; 97:21890473; 128:21890473; 11:21890473,21906983; 107:21890473,21906983,22053931; 149:21906983"