PTM - CPLM Gene Name UniProt ID Type Position Code ATXN3; ATX3; MJD; MJD1; SCA3 P54252 Ubiquitination 117 K ATXN3; ATX3; MJD; MJD1; SCA3 P54252 Ubiquitination 200 K ATXN3; ATX3; MJD; MJD1; SCA3 P54252 Ubiquitination 190 K ATXN3; ATX3; MJD; MJD1; SCA3 P54252 Ubiquitination 206 K ATXN3; ATX3; MJD; MJD1; SCA3 P54252 Ubiquitination 291 K

PTM - dbPAF Gene Name UniProt ID Position Type Species ATXN3; ATX3; MJD; MJD1; SCA3 P54252 219 S Homo sapiens(Human) ATXN3; ATX3; MJD; MJD1; SCA3 P54252 236 S Homo sapiens(Human) ATXN3; ATX3; MJD; MJD1; SCA3 P54252 256 S Homo sapiens(Human) ATXN3; ATX3; MJD; MJD1; SCA3 P54252 260 S Homo sapiens(Human) ATXN3; ATX3; MJD; MJD1; SCA3 P54252 261 S Homo sapiens(Human) ATXN3; ATX3; MJD; MJD1; SCA3 P54252 265 S Homo sapiens(Human) ATXN3; ATX3; MJD; MJD1; SCA3 P54252 269 T Homo sapiens(Human) ATXN3; ATX3; MJD; MJD1; SCA3 P54252 272 S Homo sapiens(Human) ATXN3; ATX3; MJD; MJD1; SCA3 P54252 58 Y Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P54252 S12-p Phosphorylation FHEkQEGsLCAQHCL P54252 S55-p Phosphorylation MAEGGVTsEDyRtFL P54252 Y58-p Phosphorylation GGVTsEDyRtFLQQP P54252 T60-p Phosphorylation VTsEDyRtFLQQPSG P54252 S219-p Phosphorylation VLEANDGsGMLDEDE P54252 S236-p Phosphorylation LQRALALsRQEIDME P54252 S256-p Phosphorylation LRRAIQLsMQGsSRN P54252 S260-p Phosphorylation IQLsMQGsSRNIsQD P54252 S265-p Phosphorylation QGsSRNIsQDMtQTS P54252 T269-p Phosphorylation RNIsQDMtQTSGTNL P54252 S278-p Phosphorylation TSGTNLTsEELRKRR P54252 K166-sm Sumoylation GYSIFVVkGDLPDCE P54252 K8-ub Ubiquitination MESIFHEkQEGsLCA P54252 K117-ub Ubiquitination RSFICNYkEHWFTVR P54252 K190-ub Ubiquitination VQQMHRPkLIGEELA P54252 K200-ub Ubiquitination GEELAQLkEQRVHkT P54252 K206-ub Ubiquitination LkEQRVHkTDLERVL P54252 K291-ub Ubiquitination RREAYFEkQQQKQQQ

PTM - dbPTM UniProt ID Position Code Type Resources References P54252 256 S Phosphorylation PhosphoELM.10011 17434145 P54252 117 K Ubiquitylation Phosphositeplus.1010730 20639865;21890473;21906983 P54252 190 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54252 200 K Ubiquitylation Phosphositeplus.1010730 20639865;21890473;21906983 P54252 206 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54252 291 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P54252 256 S Phosphorylation Phosphositeplus.1010730 17434145 P54252 265 S Phosphorylation HPRD 9.0 18077418

PTM - HPRD Symbol Position Code Type References ATXN3 210 S Phosphorylation 18077418 ATXN3 265 S Phosphorylation 18077418

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P54252 256 S 17434145 GSK-3_beta LTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P54252 265 Phosphoserine 23186163

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 4287 110433 - K Ubiquitination 16040601 4287 110433 - K Ubiquitination 14749733 4287 110433 - K Ubiquitination 19661182 4287 110433 - K Ubiquitination 20639865 4287 110433 - K Ubiquitination 22118674 4287 110433 - K Ubiquitination 18781797 4287 110433 - K Ubiquitination 21987572 4287 110433 - K Ubiquitination 21139048 4287 110433 - K Ubiquitination 21890473 4287 110433 - K Ubiquitination 21906983 4287 110433 - K Ubiquitination 21625540 4287 110433 - K Ubiquitination 14559776 4287 110433 - K Ubiquitination 22970133 4287 110433 - K Ubiquitination 22053931 4287 110433 - K Neddylation 17935801 4287 110433 - K Ubiquitination 22505724 4287 110433 - K Sumoylation 23382880 4287 110433 - K Ubiquitination 23382880 4287 110433 - K Ubiquitination 19153604 4287 110433 - K Ubiquitination 21963094 4287 110433 - K Ubiquitination 23000965 4287 110433 - K Ubiquitination 24654937 4287 110433 - K Ubiquitination 24769000 4287 110433 - K Ubiquitination 24865853 4287 110433 - K Ubiquitination 25015289 4287 110433 - K Neddylation 25231079 4287 110433 - K Ubiquitination 25231079 4287 110433 - K Ubiquitination 25144244 4287 110433 - K Ubiquitination 25147182 4287 110433 - K Ubiquitination 12938929 4287 110433 - K FAT10ylation 21757738

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID ATXN3 Homo sapiens (Human) 140; 124; 225; 134 "140,KEQRVHKTDLERV; 124,QQMHRPKLIGEEL; 225,REAYFEKQQQKQQ; 134,EELAQLKEQRVHK" 140:21890473; 124:21890473; 225:21890473; 134:21890473 ATXN3 Homo sapiens (Human) 236; 145; 62; 135; 151 "236,REAYFEKQQQKQQ; 145,EELAQLKEQRVHK; 62,SFICNYKEHWFTV; 135,QQMHRPKLIGEEL; 151,KEQRVHKTDLERV" 236:21890473; 145:21890473; 62:21890473; 135:21890473; 151:21890473 ATXN3 Homo sapiens (Human) 117; 206; 291; 190; 200 "117,SFICNYKEHWFTV; 206,KEQRVHKTDLERV; 291,REAYFEKQQQKQQ; 190,QQMHRPKLIGEEL; 200,EELAQLKEQRVHK" "117:21890473; 206:21890473; 291:21890473; 190:21890473; 200:21890473,21139048" ATXN3 Homo sapiens (Human) 117; 206; 291; 190; 200 "117,SFICNYKEHWFTV; 206,KEQRVHKTDLERV; 291,REAYFEKQQQKQQ; 190,QQMHRPKLIGEEL; 200,EELAQLKEQRVHK" 117:21906983; 206:21906983; 291:21906983; 190:21906983; 200:21906983 ATXN3 Homo sapiens (Human) 117; 206; 190; 200 "117,SFICNYKEHWFTV; 206,KEQRVHKTDLERV; 190,QQMHRPKLIGEEL; 200,EELAQLKEQRVHK" 117:21906983; 206:21906983; 190:21906983; 200:21906983 ATXN3 Homo sapiens (Human) 117; 206; 190; 200 "117,SFICNYKEHWFTV; 206,KEQRVHKTDLERV; 190,QQMHRPKLIGEEL; 200,EELAQLKEQRVHK" 117:21906983; 206:21906983; 190:21906983; 200:21906983 ATXN3 Homo sapiens (Human) 117; 206; 190; 200 "117,SFICNYKEHWFTV; 206,KEQRVHKTDLERV; 190,QQMHRPKLIGEEL; 200,EELAQLKEQRVHK" 117:21906983; 206:21906983; 190:21906983; 200:21906983 ATXN3 Homo sapiens (Human) 139; 155; 149; 240 "139,QQMHRPKLIGEEL; 155,KEQRVHKTDLERV; 149,EELAQLKEQRVHK; 240,REAYFEKQQQKQQ" 139:21906983; 155:21906983; 149:21906983; 240:21906983 ATXN3 Homo sapiens (Human) 236; 145; 62; 135; 151 "236,REAYFEKQQQKQQ; 145,EELAQLKEQRVHK; 62,SFICNYKEHWFTV; 135,QQMHRPKLIGEEL; 151,KEQRVHKTDLERV" 236:21890473; 145:21890473; 62:21890473; 135:21890473; 151:21890473 ATXN3 Homo sapiens (Human) 206 "206,KEQRVHKTDLERV" 206:22053931 ATXN3 Homo sapiens (Human) 236; 145; 62; 135; 151 "236,REAYFEKQQQKQQ; 145,EELAQLKEQRVHK; 62,SFICNYKEHWFTV; 135,QQMHRPKLIGEEL; 151,KEQRVHKTDLERV" "236:21890473,21906983; 145:21890473,21906983; 62:21890473,21906983; 135:21890473,21906983; 151:21890473,21906983" ATXN3 Homo sapiens (Human) 117; 206; 291; 190; 200 "117,SFICNYKEHWFTV; 206,KEQRVHKTDLERV; 291,REAYFEKQQQKQQ; 190,QQMHRPKLIGEEL; 200,EELAQLKEQRVHK" "117:21890473,21906983; 206:21890473,21906983,22053931; 291:21890473,21906983; 190:21890473,21906983; 200:21890473,21906983" ATXN3 Homo sapiens (Human) 117; 206; 291; 190; 200 "117,SFICNYKEHWFTV; 206,KEQRVHKTDLERV; 291,REAYFEKQQQKQQ; 190,QQMHRPKLIGEEL; 200,EELAQLKEQRVHK" "117:21890473,21906983; 206:21890473,21906983; 291:21890473,21906983; 190:21890473,21906983; 200:21890473,21906983"