PTM - CPLM Gene Name UniProt ID Type Position Code WDR1 O75083 Acetylation 28 K WDR1 O75083 Acetylation 81 K WDR1 O75083 Acetylation 95 K WDR1 O75083 Acetylation 115 K WDR1 O75083 Acetylation 480 K WDR1 O75083 Acetylation 511 K WDR1 O75083 Acetylation 256 K WDR1 O75083 Acetylation 321 K WDR1 O75083 Acetylation 569 K WDR1 O75083 Ubiquitination 7 K WDR1 O75083 Ubiquitination 28 K WDR1 O75083 Ubiquitination 38 K WDR1 O75083 Ubiquitination 90 K WDR1 O75083 Ubiquitination 95 K WDR1 O75083 Ubiquitination 405 K WDR1 O75083 Ubiquitination 21 K WDR1 O75083 Ubiquitination 106 K WDR1 O75083 Ubiquitination 115 K WDR1 O75083 Ubiquitination 437 K WDR1 O75083 Ubiquitination 180 K WDR1 O75083 Ubiquitination 489 K WDR1 O75083 Ubiquitination 480 K WDR1 O75083 Ubiquitination 334 K WDR1 O75083 Ubiquitination 223 K WDR1 O75083 Ubiquitination 155 K WDR1 O75083 Ubiquitination 569 K WDR1 O75083 Ubiquitination 104 K WDR1 O75083 Ubiquitination 219 K WDR1 O75083 Ubiquitination 321 K WDR1 O75083 Ubiquitination 81 K WDR1 O75083 Ubiquitination 362 K WDR1 O75083 Ubiquitination 484 K WDR1 O75083 Ubiquitination 126 K WDR1 O75083 Ubiquitination 311 K WDR1 O75083 Ubiquitination 317 K WDR1 O75083 Ubiquitination 231 K WDR1 O75083 Ubiquitination 182 K WDR1 O75083 Ubiquitination 256 K

PTM - dbPAF Gene Name UniProt ID Position Type Species WDR1 O75083 11 S Homo sapiens(Human) WDR1 O75083 164 T Homo sapiens(Human) WDR1 O75083 20 S Homo sapiens(Human) WDR1 O75083 238 Y Homo sapiens(Human) WDR1 O75083 241 S Homo sapiens(Human) WDR1 O75083 3 Y Homo sapiens(Human) WDR1 O75083 300 Y Homo sapiens(Human) WDR1 O75083 34 Y Homo sapiens(Human) WDR1 O75083 348 Y Homo sapiens(Human) WDR1 O75083 358 S Homo sapiens(Human) WDR1 O75083 392 S Homo sapiens(Human) WDR1 O75083 398 Y Homo sapiens(Human) WDR1 O75083 399 S Homo sapiens(Human) WDR1 O75083 472 Y Homo sapiens(Human) WDR1 O75083 560 T Homo sapiens(Human) WDR1 O75083 72 Y Homo sapiens(Human) WDR1 O75083 75 S Homo sapiens(Human) WDR1 O75083 79 S Homo sapiens(Human) WDR1 O75083 96 Y Homo sapiens(Human) WDR1 O75083 98 Y Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA O75083 K28-ac Acetylation kIIGGDPkGNNFLyT O75083 K38-ac Acetylation NFLyTNGkCVILRNI O75083 K81-ac Acetylation AsGDVsGkLrIWDTT O75083 K95-ac Acetylation TQkEHLLkyEyQPFA O75083 K104-ac Acetylation EyQPFAGkIkDIAWT O75083 K115-ac Acetylation IAWTEDSkRIAVVGE O75083 K180-ac Acetylation FFEGPPFkFkFTIGD O75083 K182-ac Acetylation EGPPFkFkFTIGDHS O75083 K219-ac Acetylation QIyIYDGkTGEkVCA O75083 K223-ac Acetylation YDGkTGEkVCALGGS O75083 K256-ac Acetylation LLSASGDkTSKIWDV O75083 K321-ac Acetylation HVIKGHSksIQCLTV O75083 K330-ac Acetylation IQCLTVHkNGGkSYI O75083 K480-ac Acetylation sILGTTLkDEGkLLE O75083 K569-ac Acetylation SDPETRVkIQDAHRL O75083 R17-m1 Methylation AsLPQVErGVskIIG O75083 R83-m1 Methylation GDVsGkLrIWDTTQk O75083 R396-m1 Methylation RYTsLMLrDysGQGV O75083 Y3-p Phosphorylation _____MPyEIKkVFA O75083 S11-p Phosphorylation EIKkVFAsLPQVErG O75083 S20-p Phosphorylation PQVErGVskIIGGDP O75083 Y34-p Phosphorylation PkGNNFLyTNGkCVI O75083 Y54-p Phosphorylation NPALADIytEHAHQV O75083 T55-p Phosphorylation PALADIytEHAHQVV O75083 Y72-p Phosphorylation KYAPSGFyIAsGDVs O75083 S75-p Phosphorylation PSGFyIAsGDVsGkL O75083 S79-p Phosphorylation yIAsGDVsGkLrIWD O75083 Y96-p Phosphorylation QkEHLLkyEyQPFAG O75083 Y98-p Phosphorylation EHLLkyEyQPFAGkI O75083 S151-p Phosphorylation GHNKVINsVDIkQSR O75083 T164-p Phosphorylation SRPYRLAtGsDDNCA O75083 S166-p Phosphorylation PYRLAtGsDDNCAAF O75083 Y214-p Phosphorylation ASADGQIyIYDGkTG O75083 Y238-p Phosphorylation KAHDGGIyAIsWSPD O75083 S241-p Phosphorylation DGGIyAIsWSPDSTH O75083 Y300-p Phosphorylation LSVSLSGyINYLDRN O75083 S310-p Phosphorylation YLDRNNPskPLHVIK O75083 S322-p Phosphorylation VIKGHSksIQCLTVH O75083 Y348-p Phosphorylation SHDGHINyWDsETGE O75083 S351-p Phosphorylation GHINyWDsETGENDs O75083 S358-p Phosphorylation sETGENDsFAGkGHT O75083 S383-p Phosphorylation SGQLISCsMDDTVRY O75083 S392-p Phosphorylation DDTVRYTsLMLrDys O75083 Y398-p Phosphorylation TsLMLrDysGQGVVk O75083 S399-p Phosphorylation sLMLrDysGQGVVkL O75083 Y472-p Phosphorylation VDGNVRLysILGTTL O75083 S473-p Phosphorylation DGNVRLysILGTTLk O75083 S580-p Phosphorylation AHRLHHVssLAWLDE O75083 S581-p Phosphorylation HRLHHVssLAWLDEH O75083 K7-ub Ubiquitination _MPyEIKkVFAsLPQ O75083 K21-ub Ubiquitination QVErGVskIIGGDPk O75083 K28-ub Ubiquitination kIIGGDPkGNNFLyT O75083 K38-ub Ubiquitination NFLyTNGkCVILRNI O75083 K81-ub Ubiquitination AsGDVsGkLrIWDTT O75083 K90-ub Ubiquitination rIWDTTQkEHLLkyE O75083 K95-ub Ubiquitination TQkEHLLkyEyQPFA O75083 K104-ub Ubiquitination EyQPFAGkIkDIAWT O75083 K106-ub Ubiquitination QPFAGkIkDIAWTED O75083 K115-ub Ubiquitination IAWTEDSkRIAVVGE O75083 K155-ub Ubiquitination VINsVDIkQSRPYRL O75083 K180-ub Ubiquitination FFEGPPFkFkFTIGD O75083 K219-ub Ubiquitination QIyIYDGkTGEkVCA O75083 K223-ub Ubiquitination YDGkTGEkVCALGGS O75083 K256-ub Ubiquitination LLSASGDkTSKIWDV O75083 K311-ub Ubiquitination LDRNNPskPLHVIKG O75083 K321-ub Ubiquitination HVIKGHSksIQCLTV O75083 K334-ub Ubiquitination TVHkNGGkSYIYSGS O75083 K362-ub Ubiquitination ENDsFAGkGHTNQVS O75083 K405-ub Ubiquitination ysGQGVVkLDVQPKC O75083 K480-ub Ubiquitination sILGTTLkDEGkLLE O75083 K484-ub Ubiquitination TTLkDEGkLLEAkGP O75083 K489-ub Ubiquitination EGkLLEAkGPVTDVA

PTM - dbPTM UniProt ID Position Code Type Resources References O75083 28 K Acetylation Swiss-Prot 1010711 19608861 O75083 81 K Acetylation Swiss-Prot 1010711 19608861 O75083 95 K Acetylation Swiss-Prot 1010711 19608861 O75083 115 K Acetylation Swiss-Prot 1010711 19608861 O75083 238 Y Phosphorylation Swiss-Prot 1010711 15592455;19690332 O75083 480 K Acetylation Swiss-Prot 1010711 19608861 O75083 238 Y Phosphorylation PhosphoELM.10011 15592455 O75083 115 K Ubiquitylation Phosphositeplus.1010730 21906983 O75083 21 K Ubiquitylation Phosphositeplus.1010730 21906983 O75083 219 K Ubiquitylation Phosphositeplus.1010730 21906983 O75083 223 K Ubiquitylation Phosphositeplus.1010730 21906983 O75083 256 K Ubiquitylation Phosphositeplus.1010730 21906983 O75083 28 K Ubiquitylation Phosphositeplus.1010730 21890473 O75083 362 K Ubiquitylation Phosphositeplus.1010730 21906983 O75083 38 K Ubiquitylation Phosphositeplus.1010730 21890473 O75083 405 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 O75083 480 K Ubiquitylation Phosphositeplus.1010730 21906983 O75083 484 K Ubiquitylation Phosphositeplus.1010730 21906983 O75083 7 K Ubiquitylation Phosphositeplus.1010730 21890473 O75083 90 K Ubiquitylation Phosphositeplus.1010730 21890473 O75083 95 K Ubiquitylation Phosphositeplus.1010730 21890473 O75083 115 K Acetylation Phosphositeplus.1010730 19608861 O75083 28 K Acetylation Phosphositeplus.1010730 19608861 O75083 480 K Acetylation Phosphositeplus.1010730 19608861 O75083 81 K Acetylation Phosphositeplus.1010730 19608861 O75083 95 K Acetylation Phosphositeplus.1010730 19608861 O75083 20 S Phosphorylation Phosphositeplus.1010730 20068231 O75083 238 Y Phosphorylation Phosphositeplus.1010730 15592455;16464493;17360941;18083107;18180459;19605366;19690332;20090780;20736484;21394647 O75083 72 Y Phosphorylation Phosphositeplus.1010730 17360941;18083107;20090780;21253578 O75083 28 K Acetylation HPRD 9.0 19608861 O75083 81 K Acetylation HPRD 9.0 19608861 O75083 95 K Acetylation HPRD 9.0 19608861 O75083 115 K Acetylation HPRD 9.0 19608861 O75083 480 K Acetylation HPRD 9.0 19608861 O75083 511 K Acetylation HPRD 9.0 19608861 O75083 20 S Phosphorylation HPRD 9.0 20068231 O75083 98 Y Phosphorylation HPRD 9.0 15592455 O75083 238 Y Phosphorylation HPRD 9.0 15592455

PTM - HPRD Symbol Position Code Type References WDR1 115 K Acetylation 19608861 WDR1 20 S Phosphorylation 20068231 WDR1 238 Y Phosphorylation 15592455 WDR1 28 K Acetylation 19608861 WDR1 340 K Acetylation 19608861 WDR1 371 K Acetylation 19608861 WDR1 480 K Acetylation 19608861 WDR1 511 K Acetylation 19608861 WDR1 81 K Acetylation 19608861 WDR1 95 K Acetylation 19608861 WDR1 98 Y Phosphorylation 15592455

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type O75083 238 Y 15592455 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs O75083 28 N6-acetyllysine 19608861 O75083 81 N6-acetyllysine 19608861 O75083 95 N6-acetyllysine 19608861 O75083 115 N6-acetyllysine 19608861 O75083 238 Phosphotyrosine 15592455;19690332 O75083 480 N6-acetyllysine 19608861

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type WDR1 IPI00746165 K 115 AWTEDSaKRIAVVG acetylation WDR1 IPI00746165 K 28 IIGGDPaKGNNFLY acetylation WDR1 IPI00746165 K 480 ILGTTLaKDEGKLL acetylation WDR1 IPI00746165 K 511 AVCDASaKVVTVFS acetylation WDR1 IPI00746165 K 81 SGDVSGaKLRIWDT acetylation WDR1 IPI00746165 K 95 QKEHLLaKYEYQPF acetylation WDR1 IPI00746165 S 20 QVERGVpSKIIGGD phosphorylation WDR1 IPI00746165 T 164 RPYRLApTGSDDNC phosphorylation WDR1 IPI00789936 T 11 LPQPVLpTARYTSM phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 9948 115273 - K Ubiquitination 18781797 9948 115273 - K Ubiquitination 21963094 9948 115273 - K Ubiquitination 21139048 9948 115273 - K Ubiquitination 21890473 9948 115273 - K Ubiquitination 21906983 9948 115273 - K Ubiquitination 22505724 9948 115273 - K Ubiquitination 23000965 9948 115273 - K Ubiquitination 24654937 9948 115273 - K Neddylation 24654937 9948 115273 - K Sumoylation 24654937 9948 115273 - K Ubiquitination 24816145 9948 115273 - K Sumoylation 25027693 9948 115273 - K Ubiquitination 25147182

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID WDR1 Homo sapiens (Human) 38; 7; 28 "38,FLYTNGKCVILRN; 7,MPYEIKKVFASLP; 28,IIGGDPKGNNFLY" 38:21890473; 7:21890473; 28:21890473 WDR1 Homo sapiens (Human) 38; 7; 95; 406; 28; 90 "38,FLYTNGKCVILRN; 7,MPYEIKKVFASLP; 95,QKEHLLKYEYQPF; 406,SGQGVVKLDVQPK; 28,IIGGDPKGNNFLY; 90,IWDTTQKEHLLKY" 38:21890473; 7:21890473; 95:21890473; 406:21890473; 28:21890473; 90:21890473 WDR1 Homo sapiens (Human) 38; 7; 95; 406; 28; 90 "38,FLYTNGKCVILRN; 7,MPYEIKKVFASLP; 95,QKEHLLKYEYQPF; 406,SGQGVVKLDVQPK; 28,IIGGDPKGNNFLY; 90,IWDTTQKEHLLKY" 38:21890473; 7:21890473; 95:21890473; 406:21890473; 28:21890473; 90:21890473 WDR1 Homo sapiens (Human) 38; 7; 95; 406; 28; 90 "38,FLYTNGKCVILRN; 7,MPYEIKKVFASLP; 95,QKEHLLKYEYQPF; 406,SGQGVVKLDVQPK; 28,IIGGDPKGNNFLY; 90,IWDTTQKEHLLKY" 38:21890473; 7:21890473; 95:21890473; 406:21890473; 28:21890473; 90:21890473 WDR1 Homo sapiens (Human) 221; 327; 184; 21; 38; 445; 188; 7; 265; 28; 449 "221,LSASGDKTSKIWD; 327,NDSFAGKGHTNQV; 184,IYIYDGKTGEKVC; 21,VERGVSKIIGGDP; 38,FLYTNGKCVILRN; 445,ILGTTLKDEGKLL; 188,DGKTGEKVCALGG; 7,MPYEIKKVFASLP; 265,SGQGVVKLDVQPK; 28,IIGGDPKGNNFLY; 449,TLKDEGKLLEAKG" 221:21906983; 327:21906983; 184:21906983; 21:21906983; 38:21890473; 445:21906983; 188:21906983; 7:21890473; 265:21890473; 28:21890473; 449:21906983 WDR1 Homo sapiens (Human) 38; 7; 405; 256; 223; 21; 115; 219; 484; 95; 480; 28; 362; 90 "38,FLYTNGKCVILRN; 7,MPYEIKKVFASLP; 405,SGQGVVKLDVQPK; 256,LSASGDKTSKIWD; 223,DGKTGEKVCALGG; 21,VERGVSKIIGGDP; 115,AWTEDSKRIAVVG; 219,IYIYDGKTGEKVC; 484,TLKDEGKLLEAKG; 95,QKEHLLKYEYQPF; 480,ILGTTLKDEGKLL; 28,IIGGDPKGNNFLY; 362,NDSFAGKGHTNQV; 90,IWDTTQKEHLLKY" "38:21890473; 7:21890473; 405:21890473,21906983; 256:21906983; 223:21906983; 21:21906983; 115:21906983; 219:21906983; 484:21906983; 95:21890473; 480:21906983; 28:21890473; 362:21906983; 90:21890473" WDR1 Homo sapiens (Human) 256; 223; 21; 115; 219; 484; 480; 362; 405 "256,LSASGDKTSKIWD; 223,DGKTGEKVCALGG; 21,VERGVSKIIGGDP; 115,AWTEDSKRIAVVG; 219,IYIYDGKTGEKVC; 484,TLKDEGKLLEAKG; 480,ILGTTLKDEGKLL; 362,NDSFAGKGHTNQV; 405,SGQGVVKLDVQPK" 256:21906983; 223:21906983; 21:21906983; 115:21906983; 219:21906983; 484:21906983; 480:21906983; 362:21906983; 405:21906983 WDR1 Homo sapiens (Human) 38; 7; 265; 28 "38,FLYTNGKCVILRN; 7,MPYEIKKVFASLP; 265,SGQGVVKLDVQPK; 28,IIGGDPKGNNFLY" 38:21890473; 7:21890473; 265:21890473; 28:21890473 WDR1 Homo sapiens (Human) 38; 113; 7; 25; 108; 95; 56; 46; 406; 28; 423; 90 "38,FLYTNGKCVILRN; 113,QKEHLLKYEYQPF; 7,MPYEIKKVFASLP; 25,MPYEIKKVFASLP; 108,IWDTTQKEHLLKY; 95,QKEHLLKYEYQPF; 56,FLYTNGKCVILRN; 46,IIGGDPKGNNFLY; 406,SGQGVVKLDVQPK; 28,IIGGDPKGNNFLY; 423,SGQGVVKLDVQPK; 90,IWDTTQKEHLLKY" 38:21890473; 7:21890473; 406:21890473; 405:21906983; 256:21906983; 223:21906983; 21:21906983; 115:21906983; 219:21906983; 484:21906983; 95:21890473; 480:21906983; 28:21890473; 90:21890473 WDR1 Homo sapiens (Human) 38; 371; 7; 28 "38,FLYTNGKCVILRN; 371,SGQGVVKLDVQPK; 7,MPYEIKKVFASLP; 28,IIGGDPKGNNFLY" 38:21890473; 371:21890473; 7:21890473; 28:21890473