PTM - CPLM Gene Name UniProt ID Type Position Code EPS15; AF1P P42566 Ubiquitination 413 K EPS15; AF1P P42566 Ubiquitination 495 K EPS15; AF1P P42566 Ubiquitination 638 K EPS15; AF1P P42566 Ubiquitination 754 K EPS15; AF1P P42566 Ubiquitination 788 K EPS15; AF1P P42566 Ubiquitination 793 K EPS15; AF1P P42566 Ubiquitination 801 K EPS15; AF1P P42566 Ubiquitination 816 K EPS15; AF1P P42566 Ubiquitination 818 K EPS15; AF1P P42566 Ubiquitination 648 K EPS15; AF1P P42566 Ubiquitination 763 K EPS15; AF1P P42566 Ubiquitination 860 K EPS15; AF1P P42566 Ubiquitination 890 K

PTM - dbPAF Gene Name UniProt ID Position Type Species EPS15; AF1P P42566 106 T Homo sapiens(Human) EPS15; AF1P P42566 107 S Homo sapiens(Human) EPS15; AF1P P42566 108 S Homo sapiens(Human) EPS15; AF1P P42566 116 S Homo sapiens(Human) EPS15; AF1P P42566 138 S Homo sapiens(Human) EPS15; AF1P P42566 140 S Homo sapiens(Human) EPS15; AF1P P42566 221 S Homo sapiens(Human) EPS15; AF1P P42566 244 S Homo sapiens(Human) EPS15; AF1P P42566 304 T Homo sapiens(Human) EPS15; AF1P P42566 315 S Homo sapiens(Human) EPS15; AF1P P42566 323 S Homo sapiens(Human) EPS15; AF1P P42566 324 S Homo sapiens(Human) EPS15; AF1P P42566 330 S Homo sapiens(Human) EPS15; AF1P P42566 435 S Homo sapiens(Human) EPS15; AF1P P42566 443 Y Homo sapiens(Human) EPS15; AF1P P42566 455 S Homo sapiens(Human) EPS15; AF1P P42566 467 S Homo sapiens(Human) EPS15; AF1P P42566 470 S Homo sapiens(Human) EPS15; AF1P P42566 485 S Homo sapiens(Human) EPS15; AF1P P42566 490 S Homo sapiens(Human) EPS15; AF1P P42566 524 T Homo sapiens(Human) EPS15; AF1P P42566 551 S Homo sapiens(Human) EPS15; AF1P P42566 558 S Homo sapiens(Human) EPS15; AF1P P42566 562 S Homo sapiens(Human) EPS15; AF1P P42566 563 S Homo sapiens(Human) EPS15; AF1P P42566 569 S Homo sapiens(Human) EPS15; AF1P P42566 595 S Homo sapiens(Human) EPS15; AF1P P42566 605 S Homo sapiens(Human) EPS15; AF1P P42566 606 S Homo sapiens(Human) EPS15; AF1P P42566 607 S Homo sapiens(Human) EPS15; AF1P P42566 609 T Homo sapiens(Human) EPS15; AF1P P42566 655 S Homo sapiens(Human) EPS15; AF1P P42566 662 S Homo sapiens(Human) EPS15; AF1P P42566 663 T Homo sapiens(Human) EPS15; AF1P P42566 668 T Homo sapiens(Human) EPS15; AF1P P42566 669 S Homo sapiens(Human) EPS15; AF1P P42566 680 S Homo sapiens(Human) EPS15; AF1P P42566 681 S Homo sapiens(Human) EPS15; AF1P P42566 683 T Homo sapiens(Human) EPS15; AF1P P42566 719 S Homo sapiens(Human) EPS15; AF1P P42566 741 S Homo sapiens(Human) EPS15; AF1P P42566 743 T Homo sapiens(Human) EPS15; AF1P P42566 744 S Homo sapiens(Human) EPS15; AF1P P42566 746 S Homo sapiens(Human) EPS15; AF1P P42566 777 T Homo sapiens(Human) EPS15; AF1P P42566 779 T Homo sapiens(Human) EPS15; AF1P P42566 790 S Homo sapiens(Human) EPS15; AF1P P42566 796 S Homo sapiens(Human) EPS15; AF1P P42566 814 S Homo sapiens(Human) EPS15; AF1P P42566 849 Y Homo sapiens(Human) EPS15; AF1P P42566 851 S Homo sapiens(Human)

PTM - PhosSNP Gene Name dbSNP ID Gene Mutation AA Mutation Mutation Position Code Peptide Score Modification Type EPS15; AF1P rs35326926 TCT->GCT S->A 562 S HQESPARSSPELLPS 562 Phosphorylation TypeIII- EPS15; AF1P rs35326926 TCT->GCT S->A 563 S QESPARSSPELLPSG 563 Phosphorylation TypeIII-

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P42566 K357-ac Acetylation NNVEQDLkEkEDTIK P42566 K359-ac Acetylation VEQDLkEkEDTIKQR P42566 K788-ac Acetylation PCPLPPGkRsINkLD P42566 K227-m1 Methylation VsPAEKAkYDEIFLk P42566 K234-m1 Methylation kYDEIFLkTDKDMDG P42566 S8-p Phosphorylation MAAAAQLsLTQLSSG P42566 T106-p Phosphorylation PPPRFHDtssPLLIS P42566 S107-p Phosphorylation PPRFHDtssPLLISG P42566 S108-p Phosphorylation PRFHDtssPLLISGT P42566 S140-p Phosphorylation DAIFDSLsPVNGFLS P42566 S221-p Phosphorylation KRKTWVVsPAEKAkY P42566 S244-p Phosphorylation KDMDGFVsGLEVREI P42566 T304-p Phosphorylation IDPPHVLtPEMIPPS P42566 S315-p Phosphorylation IPPSDRAsLQKNIIG P42566 S323-p Phosphorylation LQKNIIGssPVADFS P42566 S324-p Phosphorylation QKNIIGssPVADFSA P42566 S435-p Phosphorylation SLKAELTsQESQIST P42566 Y443-p Phosphorylation QESQISTyEEELAKA P42566 S455-p Phosphorylation AKAREELsRLQQETA P42566 S467-p Phosphorylation ETAELEEsVEsGKAQ P42566 S470-p Phosphorylation ELEEsVEsGKAQLEP P42566 S485-p Phosphorylation LQQHLQDsQQEISSM P42566 S551-p Phosphorylation EGQSNLEsEPIHQEs P42566 S558-p Phosphorylation sEPIHQEsPARssPE P42566 S562-p Phosphorylation HQEsPARssPELLPS P42566 S563-p Phosphorylation QEsPARssPELLPSG P42566 S587-p Phosphorylation AVTEKVCsELDNNRH P42566 S595-p Phosphorylation ELDNNRHsKEEDPFN P42566 S605-p Phosphorylation EDPFNVDssSLTGPV P42566 S606-p Phosphorylation DPFNVDssSLTGPVA P42566 S655-p Phosphorylation kGSDPFAsDCFFRQS P42566 T663-p Phosphorylation DCFFRQStDPFATSS P42566 S680-p Phosphorylation PFSAANNssITSVET P42566 S681-p Phosphorylation FSAANNssITSVETL P42566 S719-p Phosphorylation ASVFGNEsFGGGFAD P42566 S741-p Phosphorylation NNEDPFRsATSSsVS P42566 S746-p Phosphorylation FRsATSSsVSNVVIT P42566 T777-p Phosphorylation ALPPKIGtPtRPCPL P42566 T779-p Phosphorylation PPKIGtPtRPCPLPP P42566 S790-p Phosphorylation PLPPGkRsINkLDsP P42566 S796-p Phosphorylation RsINkLDsPDPFkLN P42566 S814-p Phosphorylation QPFPGNDsPkEkDPE P42566 Y849-p Phosphorylation NFANFSAyPsEEDMI P42566 S851-p Phosphorylation ANFSAyPsEEDMIEW P42566 K333-ub Ubiquitination VADFSAIkELDTLNN P42566 K413-ub Ubiquitination AQLEEQLkEVRKKCA P42566 K495-ub Ubiquitination EISSMQMkLMEMKDL P42566 K638-ub Ubiquitination FKDDPFGkIDPFGGD P42566 K648-ub Ubiquitination PFGGDPFkGSDPFAs P42566 K754-ub Ubiquitination VSNVVITkNVFEETS P42566 K763-ub Ubiquitination VFEETSVkSEDEPPA P42566 K788-ub Ubiquitination PCPLPPGkRsINkLD P42566 K793-ub Ubiquitination PGkRsINkLDsPDPF P42566 K801-ub Ubiquitination LDsPDPFkLNDPFQP P42566 K816-ub Ubiquitination FPGNDsPkEkDPEIF P42566 K818-ub Ubiquitination GNDsPkEkDPEIFCD P42566 K860-ub Ubiquitination EDMIEWAkRESEREE P42566 K890-ub Ubiquitination ELAIALSkSEISEA_

PTM - dbPTM UniProt ID Position Code Type Resources References P42566 108 S Phosphorylation Swiss-Prot 1010711 19690332 P42566 140 S Phosphorylation Swiss-Prot 1010711 18669648 P42566 323 S Phosphorylation Swiss-Prot 1010711 16964243;18669648;19690332 P42566 324 S Phosphorylation Swiss-Prot 1010711 18088087;18691976;19690332 P42566 485 S Phosphorylation Swiss-Prot 1010711 17525332;18669648 P42566 777 T Phosphorylation Swiss-Prot 1010711 18088087 P42566 796 S Phosphorylation Swiss-Prot 1010711 18088087 P42566 814 S Phosphorylation Swiss-Prot 1010711 17081983;18088087;18669648;18691976;19690332 P42566 849 Y Phosphorylation Swiss-Prot 1010711 15951569;17389395;19534553;19690332 P42566 140 S Phosphorylation PhosphoELM.10011 18669648 P42566 323 S Phosphorylation PhosphoELM.10011 18669648;16964243 P42566 324 S Phosphorylation PhosphoELM.10011 18088087 P42566 485 S Phosphorylation PhosphoELM.10011 18669648 P42566 562 S Phosphorylation PhosphoELM.10011 18669648;15302935 P42566 563 S Phosphorylation PhosphoELM.10011 15302935 P42566 595 S Phosphorylation PhosphoELM.10011 18669648 P42566 796 S Phosphorylation PhosphoELM.10011 18088087 P42566 814 S Phosphorylation PhosphoELM.10011 18669648;18088087;17081983 P42566 777 T Phosphorylation PhosphoELM.10011 18088087 P42566 849 Y Phosphorylation PhosphoELM.10011 15951569 P42566 413 K Ubiquitylation Phosphositeplus.1010730 21890473 P42566 495 K Ubiquitylation Phosphositeplus.1010730 21890473 P42566 638 K Ubiquitylation Phosphositeplus.1010730 21890473 P42566 754 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P42566 763 K Ubiquitylation Phosphositeplus.1010730 21906983 P42566 788 K Ubiquitylation Phosphositeplus.1010730 21890473 P42566 793 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P42566 801 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P42566 816 K Ubiquitylation Phosphositeplus.1010730 21906983 P42566 818 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P42566 860 K Ubiquitylation Phosphositeplus.1010730 21906983 P42566 890 K Ubiquitylation Phosphositeplus.1010730 21906983 P42566 107 S Phosphorylation Phosphositeplus.1010730 20058876 P42566 108 S Phosphorylation Phosphositeplus.1010730 19276368;19690332;20058876;22115753 P42566 140 S Phosphorylation Phosphositeplus.1010730 18669648;22115753 P42566 323 S Phosphorylation Phosphositeplus.1010730 16964243;18669648;19664995;19690332;22067460 P42566 324 S Phosphorylation Phosphositeplus.1010730 18088087;18691976;19664995;19690332;20068231;20562096;21406692;22115753 P42566 467 S Phosphorylation Phosphositeplus.1010730 20068231;22115753 P42566 470 S Phosphorylation Phosphositeplus.1010730 20068231;22115753 P42566 485 S Phosphorylation Phosphositeplus.1010730 17525332;18669648;20068231;21406692 P42566 562 S Phosphorylation Phosphositeplus.1010730 20044836 P42566 563 S Phosphorylation Phosphositeplus.1010730 15302935;20562096;21659604 P42566 790 S Phosphorylation Phosphositeplus.1010730 20068231;20363803;21406692;22115753 P42566 796 S Phosphorylation Phosphositeplus.1010730 18088087;19276368;19664994;20058876;20068231;20166139;20363803;20562096;21406692;22067460;22115753 P42566 814 S Phosphorylation Phosphositeplus.1010730 17081983;18088087;18669648;18691976;19276368;19362540;19664994;19690332;20058876;20068231;20164059;20363803;20562096;21406692;21659604;22115753 P42566 777 T Phosphorylation Phosphositeplus.1010730 18088087;22115753 P42566 443 Y Phosphorylation Phosphositeplus.1010730 20736484 P42566 849 Y Phosphorylation Phosphositeplus.1010730 15951569;17016520;17389395;18083107;18180459;19534553;19605366;19690332 P42566 107 S Phosphorylation HPRD 9.0 20058876 P42566 108 S Phosphorylation HPRD 9.0 20058876 P42566 116 S Phosphorylation HPRD 9.0 20068231 P42566 140 S Phosphorylation HPRD 9.0 18669648 P42566 323 S Phosphorylation HPRD 9.0 18669648,18691976,19664995,16964243,19413330,20068231 P42566 324 S Phosphorylation HPRD 9.0 17322306,18691976,18088087,19664995,19413330,20068231 P42566 467 S Phosphorylation HPRD 9.0 20068231 P42566 470 S Phosphorylation HPRD 9.0 20068231 P42566 485 S Phosphorylation HPRD 9.0 17525332,18669648,20068231 P42566 562 S Phosphorylation HPRD 9.0 18669648,19651622 P42566 595 S Phosphorylation HPRD 9.0 18669648 P42566 790 S Phosphorylation HPRD 9.0 19651622,20068231 P42566 796 S Phosphorylation HPRD 9.0 17322306,18088087,19651622,19664994,18767875,19413330,20166139,20068231,20058876 P42566 814 S Phosphorylation HPRD 9.0 17322306,18669648,18691976,18088087,19651622,19664994,18767875,20068231,20058876 P42566 777 T Phosphorylation HPRD 9.0 18088087 P42566 849 Y Phosphorylation (EGFR) HPRD 9.0 10953014,17389395,17016520 P42566 140 S Phosphorylation SysPTM 1.1 18669648 P42566 323 S Phosphorylation SysPTM 1.1 16964243;18669648 P42566 324 S Phosphorylation SysPTM 1.1 18691976;18088087 P42566 485 S Phosphorylation SysPTM 1.1 18669648 P42566 796 S Phosphorylation SysPTM 1.1 18088087 P42566 814 S Phosphorylation SysPTM 1.1 17081983;18691976;18669648;18088087 P42566 777 T Phosphorylation SysPTM 1.1 18088087

PTM - HPRD Symbol Position Code Type References EPS15 107 S Phosphorylation 20058876 EPS15 108 S Phosphorylation 20058876 EPS15 116 S Phosphorylation 20068231 EPS15 140 S Phosphorylation 18669648 EPS15 323 S Phosphorylation 18669648,18691976,19664995,16964243,19413330,20068231 EPS15 324 S Phosphorylation 17322306,18691976,18088087,19664995,19413330,20068231 EPS15 467 S Phosphorylation 20068231 EPS15 470 S Phosphorylation 20068231 EPS15 485 S Phosphorylation 17525332,18669648,20068231 EPS15 562 S Phosphorylation 18669648,19651622 EPS15 595 S Phosphorylation 18669648 EPS15 777 T Phosphorylation 18088087 EPS15 790 S Phosphorylation 19651622,20068231 EPS15 796 S Phosphorylation 17322306,18088087,19651622,19664994,18767875,19413330,20166139,20068231,20058876 EPS15 814 S Phosphorylation 17322306,18669648,18691976,18088087,19651622,19664994,18767875,20068231,20058876 EPS15 849 Y Phosphorylation 10953014,17389395,17016520

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P42566 485 S 18669648 HTP Phosphorylation P42566 323 S 18669648 HTP Phosphorylation P42566 323 S 16964243 HTP Phosphorylation P42566 140 S 18669648 HTP Phosphorylation P42566 324 S 18088087 HTP Phosphorylation P42566 849 Y 15951569 HTP Phosphorylation P42566 814 S 18669648 HTP Phosphorylation P42566 814 S 18088087 HTP Phosphorylation P42566 814 S 17081983 HTP Phosphorylation P42566 777 T 18088087 HTP Phosphorylation P42566 562 S 18669648 HTP Phosphorylation P42566 562 S 15302935 HTP Phosphorylation P42566 796 S 18088087 HTP Phosphorylation P42566 595 S 18669648 HTP Phosphorylation P42566 563 S 15302935 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P42566 2 N-acetylalanine 19413330;22223895;22814378 P42566 108 Phosphoserine 19690332 P42566 140 Phosphoserine 18669648 P42566 323 Phosphoserine 16964243;18669648;19690332 P42566 324 Phosphoserine 19690332;20068231;21406692;23186163;24275569 P42566 467 Phosphoserine 20068231 P42566 470 Phosphoserine 20068231 P42566 485 Phosphoserine 17525332;18669648;21406692 P42566 746 Phosphoserine 23186163 P42566 777 Phosphothreonine 23186163;24275569 P42566 790 Phosphoserine 20068231;21406692;23186163 P42566 796 Phosphoserine 18088087;20068231;21406692;23186163;24275569 P42566 814 Phosphoserine 18088087;18669648;19690332;20068231;21406692;23186163;24275569 P42566 849 Phosphotyrosine; by EGFR 19690332

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type EPS15 IPI00292134 S 324 KNIIGSpSPVADFS phosphorylation EPS15 IPI00292134 S 467 TAELEEpSVESGKA phosphorylation EPS15 IPI00292134 S 470 LEESVEpSGKAQLE phosphorylation EPS15 IPI00292134 S 485 QQHLQDpSQQEISS phosphorylation EPS15 IPI00292134 S 790 LPPGKRpSINKLDS phosphorylation EPS15 IPI00292134 S 796 SINKLDpSPDPFKL phosphorylation EPS15 IPI00292134 S 814 PFPGNDpSPKEKDP phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 2060 108374 - K Ubiquitination 16771824 2060 108374 - K Ubiquitination 16735510 2060 108374 - K Ubiquitination 12072436 2060 108374 - K Ubiquitination 9162018 2060 108374 - K Ubiquitination 17013377 2060 108374 - K Ubiquitination 19109251 2060 108374 - K Ubiquitination 21987572 2060 108374 - K Ubiquitination 21139048 2060 108374 - K Ubiquitination 21890473 2060 108374 - K Ubiquitination 21906983 2060 108374 - K Ubiquitination 15809337 2060 108374 - K Ubiquitination 22505724 2060 108374 - K Ubiquitination 21963094 2060 108374 - K Ubiquitination 23000965 2060 108374 - K Ubiquitination 24654937 2060 108374 - K Ubiquitination 24816145 2060 108374 - K Ubiquitination 25147182 2060 108374 - K Ubiquitination 25292214 2060 108374 - K Ubiquitination 22261743 2060 108374 - K Ubiquitination 12938929 2060 108374 - K Ubiquitination 16429130

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID EPS15 Homo sapiens (Human) 440; 479; 324; 474; 504; 99; 487; 502; 181 "440,SNVVITKNVFEET; 479,GKRSINKLDSPDP; 324,KDDPFGKIDPFGG; 474,CPLPPGKRSINKL; 504,NDSPKEKDPEIFC; 99,QLEEQLKEVRKKC; 487,DSPDPFKLNDPFQ; 502,PGNDSPKEKDPEI; 181,ISSMQMKLMEMKD" 440:21890473; 479:21890473; 324:21890473; 474:21890473; 504:21890473; 99:21890473; 487:21890473; 502:21890473; 181:21890473 EPS15 Homo sapiens (Human) 654; 684; 504; 682; 413; 667; 620; 659 "654,CPLPPGKRSINKL; 684,NDSPKEKDPEIFC; 504,KDDPFGKIDPFGG; 682,PGNDSPKEKDPEI; 413,QLEEQLKEVRKKC; 667,DSPDPFKLNDPFQ; 620,SNVVITKNVFEET; 659,GKRSINKLDSPDP" 654:21890473; 684:21890473; 504:21890473; 682:21890473; 413:21890473; 667:21890473; 620:21890473; 659:21890473 EPS15 Homo sapiens (Human) 654; 684; 504; 682; 413; 667; 620; 659 "654,CPLPPGKRSINKL; 684,NDSPKEKDPEIFC; 504,KDDPFGKIDPFGG; 682,PGNDSPKEKDPEI; 413,QLEEQLKEVRKKC; 667,DSPDPFKLNDPFQ; 620,SNVVITKNVFEET; 659,GKRSINKLDSPDP" 654:21890473; 684:21890473; 504:21890473; 682:21890473; 413:21890473; 667:21890473; 620:21890473; 659:21890473 EPS15 Homo sapiens (Human) 763; 801; 816; 818; 754; 890; 793; 860 "763,FEETSVKSEDEPP; 801,DSPDPFKLNDPFQ; 816,PGNDSPKEKDPEI; 818,NDSPKEKDPEIFC; 754,SNVVITKNVFEET; 890,LAIALSKSEISEA; 793,GKRSINKLDSPDP; 860,DMIEWAKRESERE" 763:21906983; 801:21906983; 816:21906983; 818:21906983; 754:21906983; 890:21906983; 793:21906983; 860:21906983 EPS15 Homo sapiens (Human) 440; 479; 324; 474; 504; 99; 487; 502; 181 "440,SNVVITKNVFEET; 479,GKRSINKLDSPDP; 324,KDDPFGKIDPFGG; 474,CPLPPGKRSINKL; 504,NDSPKEKDPEIFC; 99,QLEEQLKEVRKKC; 487,DSPDPFKLNDPFQ; 502,PGNDSPKEKDPEI; 181,ISSMQMKLMEMKD" 440:21890473; 479:21890473; 324:21890473; 474:21890473; 504:21890473; 99:21890473; 487:21890473; 502:21890473; 181:21890473 EPS15 Homo sapiens (Human) 440; 576; 479; 324; 474; 504; 546; 99; 487; 502; 449; 181 "440,SNVVITKNVFEET; 576,LAIALSKSEISEA; 479,GKRSINKLDSPDP; 324,KDDPFGKIDPFGG; 474,CPLPPGKRSINKL; 504,NDSPKEKDPEIFC; 546,DMIEWAKRESERE; 99,QLEEQLKEVRKKC; 487,DSPDPFKLNDPFQ; 502,PGNDSPKEKDPEI; 449,FEETSVKSEDEPP; 181,ISSMQMKLMEMKD" "440:21890473,21906983; 576:21906983; 479:21890473,21906983; 324:21890473; 474:21890473; 504:21890473,21906983; 546:21906983; 99:21890473; 487:21890473,21906983; 502:21890473,21906983; 449:21906983; 181:21890473" EPS15 Homo sapiens (Human) 763; 495; 788; 801; 816; 413; 818; 754; 890; 638; 793; 860 "763,FEETSVKSEDEPP; 495,ISSMQMKLMEMKD; 788,CPLPPGKRSINKL; 801,DSPDPFKLNDPFQ; 816,PGNDSPKEKDPEI; 413,QLEEQLKEVRKKC; 818,NDSPKEKDPEIFC; 754,SNVVITKNVFEET; 890,LAIALSKSEISEA; 638,KDDPFGKIDPFGG; 793,GKRSINKLDSPDP; 860,DMIEWAKRESERE" "763:21906983; 495:21890473; 788:21890473; 801:21890473,21906983; 816:21890473,21906983; 413:21890473; 818:21890473,21906983; 754:21890473,21906983; 890:21906983; 638:21890473; 793:21890473,21906983; 860:21906983"