PTM - CPLM Gene Name UniProt ID Type Position Code NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 172 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 124 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 112 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 113 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 146 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 188 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 127 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 251 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 259 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 282 K NSFL1C; UBXN2C Q9UNZ2 Ubiquitination 356 K

PTM - dbPAF Gene Name UniProt ID Position Type Species NSFL1C; UBXN2C Q9UNZ2 102 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 114 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 140 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 144 T Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 145 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 155 Y Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 165 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 167 Y Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 176 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 177 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 189 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 192 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 262 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 269 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 270 T Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 271 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 272 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 29 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 354 T Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 54 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 57 T Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 73 T Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 74 S Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 95 Y Homo sapiens(Human) NSFL1C; UBXN2C Q9UNZ2 99 S Homo sapiens(Human)

PTM - PhosSNP Gene Name dbSNP ID Gene Mutation AA Mutation Mutation Position Code Peptide Score Modification Type NSFL1C; UBXN2C rs11550910 GTG->GCG V->A 140 S AVERVTKSPGETSKP 140 Phosphorylation: CDK1 TypeIII- NSFL1C; UBXN2C rs11550910 GTG->GCG V->A 140 S AVERVTKSPGETSKP 140 Phosphorylation: CDK1 TypeIII-

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9UNZ2 K146-ac Acetylation ksPGETSkPRPFAGG Q9UNZ2 K172-ac Acetylation sAyVAGEkRQHssQD Q9UNZ2 K251-ac Acetylation VkPkGAFkAFTGEGQ Q9UNZ2 R93-m1 Methylation EEEEEGQrFyAGGsE Q9UNZ2 K124-m1 Methylation ELVDDLFkGAkEHGA Q9UNZ2 R199-m1 Methylation sLDNGELrSYQDPSN Q9UNZ2 R223-m1 Methylation GEVPAELrRLAHGGQ Q9UNZ2 K247-m3 Methylation DEDFVkPkGAFkAFT Q9UNZ2 K251-m3 Methylation VkPkGAFkAFTGEGQ Q9UNZ2 S68-p Phosphorylation VSRGTAPsDNRVtsF Q9UNZ2 T73-p Phosphorylation APsDNRVtsFRDLIH Q9UNZ2 S74-p Phosphorylation PsDNRVtsFRDLIHD Q9UNZ2 Y95-p Phosphorylation EEEGQrFyAGGsERs Q9UNZ2 S99-p Phosphorylation QrFyAGGsERsGQQI Q9UNZ2 S102-p Phosphorylation yAGGsERsGQQIVGP Q9UNZ2 S114-p Phosphorylation VGPPRkksPNELVDD Q9UNZ2 S140-p Phosphorylation AVERVTksPGETSkP Q9UNZ2 Y155-p Phosphorylation RPFAGGGyRLGAAPE Q9UNZ2 S165-p Phosphorylation GAAPEEEsAyVAGEk Q9UNZ2 Y167-p Phosphorylation APEEEsAyVAGEkRQ Q9UNZ2 S176-p Phosphorylation AGEkRQHssQDVHVV Q9UNZ2 S177-p Phosphorylation GEkRQHssQDVHVVL Q9UNZ2 S189-p Phosphorylation VVLKLWksGFsLDNG Q9UNZ2 S192-p Phosphorylation KLWksGFsLDNGELr Q9UNZ2 S272-p Phosphorylation PQVLSTSsPAQQAEN Q9UNZ2 T354-p Phosphorylation ELADESQtLkEANLL Q9UNZ2 K112-ub Ubiquitination QIVGPPRkksPNELV Q9UNZ2 K113-ub Ubiquitination IVGPPRkksPNELVD Q9UNZ2 K124-ub Ubiquitination ELVDDLFkGAkEHGA Q9UNZ2 K127-ub Ubiquitination DDLFkGAkEHGAVAV Q9UNZ2 K139-ub Ubiquitination VAVERVTksPGETSk Q9UNZ2 K146-ub Ubiquitination ksPGETSkPRPFAGG Q9UNZ2 K172-ub Ubiquitination sAyVAGEkRQHssQD Q9UNZ2 K188-ub Ubiquitination HVVLKLWksGFsLDN Q9UNZ2 K245-ub Ubiquitination HRDEDFVkPkGAFkA Q9UNZ2 K251-ub Ubiquitination VkPkGAFkAFTGEGQ Q9UNZ2 K259-ub Ubiquitination AFTGEGQkLGSTAPQ Q9UNZ2 K282-ub Ubiquitination QQAENEAkASSSILI Q9UNZ2 K311-ub Ubiquitination DGGRLVQkFNHSHRI Q9UNZ2 K356-ub Ubiquitination ADESQtLkEANLLNA

PTM - dbPTM UniProt ID Position Code Type Resources References Q9UNZ2 114 S Phosphorylation Swiss-Prot 1010711 17081983;17287340;18088087;18187866;18669648;19367720;19369195;19413330;19690332 Q9UNZ2 140 S Phosphorylation Swiss-Prot 1010711 18669648 Q9UNZ2 165 S Phosphorylation Swiss-Prot 1010711 17287340 Q9UNZ2 167 Y Phosphorylation Swiss-Prot 1010711 17287340;17389395 Q9UNZ2 272 S Phosphorylation Swiss-Prot 1010711 15302935;17924679;18669648;19413330;19690332 Q9UNZ2 74 S Phosphorylation PhosphoELM.10011 18669648 Q9UNZ2 114 S Phosphorylation PhosphoELM.10011 18669648;17287340;18088087 Q9UNZ2 165 S Phosphorylation PhosphoELM.10011 17287340 Q9UNZ2 271 S Phosphorylation PhosphoELM.10011 18669648 Q9UNZ2 272 S Phosphorylation PhosphoELM.10011 18669648;15302935 Q9UNZ2 140 S Phosphorylation PhosphoELM.10011 18669648;12810701 Q9UNZ2 167 Y Phosphorylation PhosphoELM.10011 17287340 Q9UNZ2 112 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UNZ2 113 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UNZ2 124 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UNZ2 127 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UNZ2 146 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UNZ2 172 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UNZ2 188 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UNZ2 251 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UNZ2 259 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UNZ2 282 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UNZ2 356 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UNZ2 114 S Phosphorylation Phosphositeplus.1010730 17081983;17287340;17446864;18088087;18187866;18669648;18707149;19276368;19367720;19369195;19664994;19690332;20044836;20058876;20068231;20166139;20230923;20363803;20562096;20815410;20886841;21406692;21659604;22115753 Q9UNZ2 140 S Phosphorylation Phosphositeplus.1010730 12810701;18669648;20068231;20363803;21406692;22115753 Q9UNZ2 165 S Phosphorylation Phosphositeplus.1010730 17287340 Q9UNZ2 176 S Phosphorylation Phosphositeplus.1010730 20068231 Q9UNZ2 177 S Phosphorylation Phosphositeplus.1010730 20068231 Q9UNZ2 272 S Phosphorylation Phosphositeplus.1010730 15302935;16964243;17446864;18669648;19415658;19690332;20068231 Q9UNZ2 167 Y Phosphorylation Phosphositeplus.1010730 17287340;17389395;18083107;18180459;18707149;20007894;20562096;21394647;21403953 Q9UNZ2 54 S Phosphorylation HPRD 9.0 17287340 Q9UNZ2 74 S Phosphorylation HPRD 9.0 18669648 Q9UNZ2 114 S Phosphorylation HPRD 9.0 17322306,18669648,19413330,17287340,18088087,18212344,19651622,19664994,18707149,18452278,18767875,20230923,20166139,20068231,20058876 Q9UNZ2 165 S Phosphorylation HPRD 9.0 17287340 Q9UNZ2 176 S Phosphorylation HPRD 9.0 20068231 Q9UNZ2 177 S Phosphorylation HPRD 9.0 20068231 Q9UNZ2 271 S Phosphorylation HPRD 9.0 18669648 Q9UNZ2 272 S Phosphorylation HPRD 9.0 18669648,19413330,19415658,17924679,20068231 Q9UNZ2 167 Y Phosphorylation HPRD 9.0 17287340,18707149 Q9UNZ2 167 Y Phosphorylation HPRD 9.0 17389395 Q9UNZ2 29 S Phosphorylation (CDK1) HPRD 9.0 12810701,18669648,19651622,20068231 Q9UNZ2 140 S Phosphorylation (CDK1) HPRD 9.0 12810701,18669648,19651622,20068231 Q9UNZ2 140 S Phosphorylation (CDK1) HPRD 9.0 12810701 Q9UNZ2 114 S Phosphorylation SysPTM 1.1 17081983;18669648;18212344;18707149;18088087 Q9UNZ2 140 S Phosphorylation SysPTM 1.1 18669648 Q9UNZ2 272 S Phosphorylation SysPTM 1.1 18669648 Q9UNZ2 272 S Phosphorylation SysPTM 1.1 16964243 Q9UNZ2 167 Y Phosphorylation SysPTM 1.1 18707149

PTM - HPRD Symbol Position Code Type References NSFL1C 114 S Phosphorylation 17322306,18669648,19413330,17287340,18088087,18212344,19651622,19664994,18707149,18452278,18767875,20230923,20166139,20068231,20058876 NSFL1C 140 S Phosphorylation 12810701 NSFL1C 140 S Phosphorylation 12810701,18669648,19651622,20068231 NSFL1C 160 S Phosphorylation 18669648 NSFL1C 161 S Phosphorylation 18669648,19413330,19415658,17924679,20068231 NSFL1C 165 S Phosphorylation 17287340 NSFL1C 167 Y Phosphorylation 17287340,18707149 NSFL1C 167 Y Phosphorylation 17389395 NSFL1C 176 S Phosphorylation 20068231 NSFL1C 177 S Phosphorylation 20068231 NSFL1C 240 S Phosphorylation 18669648 NSFL1C 241 S Phosphorylation 18669648,19413330,19415658,17924679,20068231 NSFL1C 271 S Phosphorylation 18669648 NSFL1C 272 S Phosphorylation 18669648,19413330,19415658,17924679,20068231 NSFL1C 29 S Phosphorylation 12810701,18669648,19651622,20068231 NSFL1C 54 S Phosphorylation 17287340 NSFL1C 56 Y Phosphorylation 17287340,18707149 NSFL1C 56 Y Phosphorylation 17389395 NSFL1C 65 S Phosphorylation 20068231 NSFL1C 66 S Phosphorylation 20068231 NSFL1C 74 S Phosphorylation 18669648

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9UNZ2 167 Y 17287340 HTP Phosphorylation Q9UNZ2 165 S 17287340 HTP Phosphorylation Q9UNZ2 74 S 18669648 HTP Phosphorylation Q9UNZ2 140 S 18669648 HTP Phosphorylation Q9UNZ2 140 S 12810701 CDK1 LTP Phosphorylation Q9UNZ2 114 S 18669648 HTP Phosphorylation Q9UNZ2 114 S 17287340 HTP Phosphorylation Q9UNZ2 114 S 18088087 HTP Phosphorylation Q9UNZ2 272 S 18669648 HTP Phosphorylation Q9UNZ2 272 S 15302935 HTP Phosphorylation Q9UNZ2 271 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9UNZ2 74 Phosphoserine 23186163 Q9UNZ2 102 Phosphoserine 23186163 Q9UNZ2 114 Phosphoserine 17081983;18088087;19367720;19690332;20068231;21406692;23186163;24275569 Q9UNZ2 140 Phosphoserine 18669648;23186163 Q9UNZ2 192 Phosphoserine 23186163 Q9UNZ2 272 Phosphoserine 18669648;19690332;20068231;23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type NSFL1C IPI00022830 S 114 GPPRKKpSPNELVD phosphorylation NSFL1C IPI00100197 S 114 GPPRKKpSPNELVD phosphorylation NSFL1C IPI00100197 S 140 VERVTKpSPGETSK phosphorylation NSFL1C IPI00100197 S 272 QVLSTSpSPAQQAE phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 55968 121014 - K Ubiquitination 18781797 55968 121014 - K Ubiquitination 21987572 55968 121014 - K Ubiquitination 21139048 55968 121014 - K Ubiquitination 21890473 55968 121014 - K Ubiquitination 21906983 55968 121014 - K Ubiquitination 22053931 55968 121014 - K Ubiquitination 22505724 55968 121014 - K Ubiquitination 21963094 55968 121014 - K Ubiquitination 23000965 55968 121014 - K Ubiquitination 24654937 55968 121014 - K Ubiquitination 24816145 55968 121014 - K Ubiquitination 25147182

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID NSFL1C Homo sapiens (Human) 113; 188; 146; 124 "113,VGPPRKKSPNELV; 188,VVLKLWKSGFSLD; 146,SPGETSKPRPFAG; 124,LVDDLFKGAKEHG" 113:21890473; 188:21890473; 146:21890473; 124:21890473 NSFL1C Homo sapiens (Human) 190; 115; 188; 126; 146; 148 "190,VVLKLWKSGFSLD; 115,VGPPRKKSPNELV; 188,VVLKLWKSGFSLD; 126,LVDDLFKGAKEHG; 146,SPGETSKPRPFAG; 148,SPGETSKPRPFAG" 190:21890473; 115:21890473; 188:21906983; 126:21890473; 146:21906983; 148:21890473 NSFL1C Homo sapiens (Human) 35; 77 "35,SPGETSKPRPFAG; 77,VVLKLWKSGFSLD" 35:21890473; 77:21890473 NSFL1C Homo sapiens (Human) 356; 259; 251; 113; 188; 112; 172; 127; 282; 146; 124 "356,DESQTLKEANLLN; 259,FTGEGQKLGSTAP; 251,KPKGAFKAFTGEG; 113,VGPPRKKSPNELV; 188,VVLKLWKSGFSLD; 112,IVGPPRKKSPNEL; 172,AYVAGEKRQHSSQ; 127,DLFKGAKEHGAVA; 282,QAENEAKASSSIL; 146,SPGETSKPRPFAG; 124,LVDDLFKGAKEHG" "356:21906983; 259:21906983; 251:21906983; 113:21890473,21906983; 188:21890473,21906983; 112:21906983; 172:21906983,22053931; 127:21906983; 282:21906983; 146:21890473,21906983; 124:21890473,21906983" NSFL1C Homo sapiens (Human) 220; 325; 157; 251; 113; 112; 127; 124; 228 "220,KPKGAFKAFTGEG; 325,DESQTLKEANLLN; 157,VVLKLWKSGFSLD; 251,QAENEAKASSSIL; 113,VGPPRKKSPNELV; 112,IVGPPRKKSPNEL; 127,DLFKGAKEHGAVA; 124,LVDDLFKGAKEHG; 228,FTGEGQKLGSTAP" "220:21906983; 325:21906983; 157:21890473,21906983; 251:21906983; 113:21890473,21906983; 112:21906983; 127:21906983; 124:21890473,21906983; 228:21906983" NSFL1C Homo sapiens (Human) 358; 253; 115; 190; 114; 174; 261; 129; 126; 148; 284 "358,DESQTLKEANLLN; 253,KPKGAFKAFTGEG; 115,VGPPRKKSPNELV; 190,VVLKLWKSGFSLD; 114,IVGPPRKKSPNEL; 174,AYVAGEKRQHSSQ; 261,FTGEGQKLGSTAP; 129,DLFKGAKEHGAVA; 126,LVDDLFKGAKEHG; 148,SPGETSKPRPFAG; 284,QAENEAKASSSIL" "358:21906983; 253:21906983; 115:21890473,21906983; 190:21890473,21906983; 114:21906983; 174:21906983; 261:21906983; 129:21906983; 126:21890473,21906983; 148:21890473,21906983; 284:21906983" NSFL1C Homo sapiens (Human) 112; 172; 124 "112,IVGPPRKKSPNEL; 172,AYVAGEKRQHSSQ; 124,LVDDLFKGAKEHG" "112:21139048; 172:21139048,22053931; 124:21139048"