PTM - CPLM Gene Name UniProt ID Type Position Code CDC23; ANAPC8 Q9UJX2 Acetylation 467 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 396 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 472 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 467 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 453 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 454 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 359 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 97 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 547 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 147 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 129 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 130 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 291 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 34 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 475 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 535 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 445 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 328 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 313 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 522 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 143 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 281 K CDC23; ANAPC8 Q9UJX2 Ubiquitination 349 K

PTM - dbPAF Gene Name UniProt ID Position Type Species CDC23; ANAPC8 Q9UJX2 115 Y Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 120 Y Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 121 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 134 T Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 137 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 223 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 273 Y Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 284 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 304 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 308 Y Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 329 Y Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 340 Y Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 341 Y Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 353 Y Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 43 T Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 460 Y Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 530 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 542 T Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 562 T Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 564 T Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 565 T Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 576 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 578 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 582 T Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 584 T Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 588 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 593 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 594 S Homo sapiens(Human) CDC23; ANAPC8 Q9UJX2 596 T Homo sapiens(Human)

PTM - PhosSNP Gene Name dbSNP ID Gene Mutation AA Mutation Mutation Position Code Peptide Score Modification Type CDC23; ANAPC8 rs35119403 GCT->GGT A->G 582 T ASLSANNTPTRRVSP 576 Phosphorylation: CDK1 TypeIII-

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9UJX2 K467-ac Acetylation yAVGDVEkMALVKLA Q9UJX2 K522-m1 Methylation YLAQYYFkCKLWDEA Q9UJX2 S21-p Phosphorylation AAVAPVLsINSDFSD Q9UJX2 T43-p Phosphorylation LLLIAGLtRERGLLH Q9UJX2 Y115-p Phosphorylation GCNSKKAyFLYMysR Q9UJX2 Y120-p Phosphorylation KAyFLYMysRYLSGE Q9UJX2 S121-p Phosphorylation AyFLYMysRYLSGEK Q9UJX2 S223-p Phosphorylation KEMLKFLsLPDTWMK Q9UJX2 Y273-p Phosphorylation VSQIAVAyHNIRDID Q9UJX2 S284-p Phosphorylation RDIDkALsIFNELRK Q9UJX2 Y329-p Phosphorylation NLCEIDKyRVETCCV Q9UJX2 Y340-p Phosphorylation TCCVIGNyySLRSQH Q9UJX2 Y341-p Phosphorylation CCVIGNyySLRSQHE Q9UJX2 Y353-p Phosphorylation QHEkAALyFQRALkL Q9UJX2 Y460-p Phosphorylation kKCYWRAyAVGDVEk Q9UJX2 S530-p Phosphorylation CKLWDEAsTCAQkCC Q9UJX2 T542-p Phosphorylation kCCAFNDtREEGkAL Q9UJX2 T562-p Phosphorylation QLRNQGEtPttEVPA Q9UJX2 T564-p Phosphorylation RNQGEtPttEVPAPF Q9UJX2 T565-p Phosphorylation NQGEtPttEVPAPFF Q9UJX2 S576-p Phosphorylation APFFLPAsLsANNtP Q9UJX2 S578-p Phosphorylation FFLPAsLsANNtPtR Q9UJX2 T582-p Phosphorylation AsLsANNtPtRRVsP Q9UJX2 T584-p Phosphorylation LsANNtPtRRVsPLN Q9UJX2 S588-p Phosphorylation NtPtRRVsPLNLssV Q9UJX2 S593-p Phosphorylation RVsPLNLssVtP___ Q9UJX2 S594-p Phosphorylation VsPLNLssVtP____ Q9UJX2 T596-p Phosphorylation PLNLssVtP______ Q9UJX2 K147-sm Sumoylation PLEkGQVkNEALREL Q9UJX2 K97-ub Ubiquitination AKAYFDVkEYDRAAH Q9UJX2 K143-ub Ubiquitination DSLGPLEkGQVkNEA Q9UJX2 K147-ub Ubiquitination PLEkGQVkNEALREL Q9UJX2 K281-ub Ubiquitination HNIRDIDkALsIFNE Q9UJX2 K349-ub Ubiquitination SLRSQHEkAALyFQR Q9UJX2 K359-ub Ubiquitination LyFQRALkLNPRYLG Q9UJX2 K396-ub Ubiquitination RHAIEVNkRDYRAWY Q9UJX2 K453-ub Ubiquitination LNQLVEAkKCYWRAy Q9UJX2 K467-ub Ubiquitination yAVGDVEkMALVKLA Q9UJX2 K535-ub Ubiquitination EAsTCAQkCCAFNDt Q9UJX2 K547-ub Ubiquitination NDtREEGkALLRQIL

PTM - dbPTM UniProt ID Position Code Type Resources References Q9UJX2 273 Y Phosphorylation Swiss-Prot 1010711 14657031 Q9UJX2 467 K Acetylation Swiss-Prot 1010711 19608861 Q9UJX2 562 T Phosphorylation Swiss-Prot 1010711 14657031;18669648;19007248 Q9UJX2 565 T Phosphorylation Swiss-Prot 1010711 14657031 Q9UJX2 576 S Phosphorylation Swiss-Prot 1010711 18691976 Q9UJX2 578 S Phosphorylation Swiss-Prot 1010711 18669648;18691976;19690332 Q9UJX2 582 T Phosphorylation Swiss-Prot 1010711 19690332 Q9UJX2 584 T Phosphorylation Swiss-Prot 1010711 18669648 Q9UJX2 588 S Phosphorylation Swiss-Prot 1010711 15302935;16565220;16964243;17081983;18669648;18691976;19413330;19690332 Q9UJX2 593 S Phosphorylation Swiss-Prot 1010711 18669648 Q9UJX2 596 T Phosphorylation Swiss-Prot 1010711 16565220;16964243;17081983;18669648;18691976;19413330;19690332 Q9UJX2 588 S Phosphorylation PhosphoELM.10011 22067460;18669648 Q9UJX2 576 S Phosphorylation PhosphoELM.10011 22067460;18669648 Q9UJX2 578 S Phosphorylation PhosphoELM.10011 22067460;18669648 Q9UJX2 588 S Phosphorylation PhosphoELM.10011 22067460;18669648;16964243;17081983;15302935 Q9UJX2 593 S Phosphorylation PhosphoELM.10011 22067460;18669648;17081983 Q9UJX2 564 T Phosphorylation PhosphoELM.10011 18669648 Q9UJX2 565 T Phosphorylation PhosphoELM.10011 18669648;14657031;14657031 Q9UJX2 584 T Phosphorylation PhosphoELM.10011 22067460;18669648 Q9UJX2 582 T Phosphorylation PhosphoELM.10011 22067460;18669648;14657031 Q9UJX2 562 T Phosphorylation PhosphoELM.10011 22067460;18669648;14657031;14657031;14657031 Q9UJX2 273 Y Phosphorylation PhosphoELM.10011 14657031 Q9UJX2 143 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX2 147 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX2 281 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX2 453 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX2 467 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX2 547 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX2 97 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJX2 467 K Acetylation Phosphositeplus.1010730 19608861 Q9UJX2 284 S Phosphorylation Phosphositeplus.1010730 18420821 Q9UJX2 530 S Phosphorylation Phosphositeplus.1010730 22067460 Q9UJX2 576 S Phosphorylation Phosphositeplus.1010730 18691976;22067460 Q9UJX2 578 S Phosphorylation Phosphositeplus.1010730 18420821;18691976;19276368;19690332;22067460;22115753 Q9UJX2 588 S Phosphorylation Phosphositeplus.1010730 15302935;16565220;16964243;18420821;18669648;18691976;19664994;19664995;19690332;20068231;20363803;21406692;21659604;22067460 Q9UJX2 593 S Phosphorylation Phosphositeplus.1010730 18669648;21659604;22067460 Q9UJX2 594 S Phosphorylation Phosphositeplus.1010730 21659604;22067460 Q9UJX2 542 T Phosphorylation Phosphositeplus.1010730 22067460 Q9UJX2 562 T Phosphorylation Phosphositeplus.1010730 14657031;18420821;18669648;20068231;22067460 Q9UJX2 564 T Phosphorylation Phosphositeplus.1010730 22067460 Q9UJX2 565 T Phosphorylation Phosphositeplus.1010730 14657031;18420821 Q9UJX2 582 T Phosphorylation Phosphositeplus.1010730 18420821;18669648;19690332;22067460 Q9UJX2 584 T Phosphorylation Phosphositeplus.1010730 19276368;22067460 Q9UJX2 596 T Phosphorylation Phosphositeplus.1010730 16565220;16964243;18420821;18669648;18691976;19690332;20068231;20363803;21406692;21659604;22067460;22115753 Q9UJX2 273 Y Phosphorylation Phosphositeplus.1010730 14657031 Q9UJX2 467 K Acetylation HPRD 9.0 19608861 Q9UJX2 304 S Phosphorylation HPRD 9.0 20068231 Q9UJX2 576 S Phosphorylation HPRD 9.0 18669648,18691976,20068231 Q9UJX2 578 S Phosphorylation HPRD 9.0 15345747,18669648,18691976,20068231 Q9UJX2 588 S Phosphorylation HPRD 9.0 15302935,16565220,18669648,19413330,18691976,19664995,18212344,19651622,19664994,16964243,18767875,20068231 Q9UJX2 593 S Phosphorylation HPRD 9.0 17081983,18669648,19651622 Q9UJX2 594 S Phosphorylation HPRD 9.0 17081983,18669648,19651622,20068231 Q9UJX2 562 T Phosphorylation HPRD 9.0 18669648,19007248,20068231 Q9UJX2 564 T Phosphorylation HPRD 9.0 18669648 Q9UJX2 565 T Phosphorylation HPRD 9.0 18669648 Q9UJX2 582 T Phosphorylation HPRD 9.0 18669648,20068231 Q9UJX2 584 T Phosphorylation HPRD 9.0 18669648 Q9UJX2 596 T Phosphorylation HPRD 9.0 16565220,18669648,19413330,18691976,19651622,16964243,18767875,20068231 Q9UJX2 308 Y Phosphorylation HPRD 9.0 20068231 Q9UJX2 576 S Phosphorylation SysPTM 1.1 18691976 Q9UJX2 578 S Phosphorylation SysPTM 1.1 18691976;18669648 Q9UJX2 588 S Phosphorylation SysPTM 1.1 18691976;16964243;17081983;18669648 Q9UJX2 593 S Phosphorylation SysPTM 1.1 17081983;18669648 Q9UJX2 594 S Phosphorylation SysPTM 1.1 17081983 Q9UJX2 588 S Phosphorylation SysPTM 1.1 15302935;16565220 Q9UJX2 562 T Phosphorylation SysPTM 1.1 18669648 Q9UJX2 584 T Phosphorylation SysPTM 1.1 18669648 Q9UJX2 596 T Phosphorylation SysPTM 1.1 18691976;16964243;17081983;18669648 Q9UJX2 596 T Phosphorylation SysPTM 1.1 16565220

PTM - HPRD Symbol Position Code Type References CDC23 304 S Phosphorylation 20068231 CDC23 308 Y Phosphorylation 20068231 CDC23 467 K Acetylation 19608861 CDC23 562 T Phosphorylation 18669648,19007248,20068231 CDC23 564 T Phosphorylation 18669648 CDC23 565 T Phosphorylation 18669648 CDC23 576 S Phosphorylation 18669648,18691976,20068231 CDC23 578 S Phosphorylation 15345747,18669648,18691976,20068231 CDC23 582 T Phosphorylation 18669648,20068231 CDC23 584 T Phosphorylation 18669648 CDC23 588 S Phosphorylation 15302935,16565220,18669648,19413330,18691976,19664995,18212344,19651622,19664994,16964243,18767875,20068231 CDC23 593 S Phosphorylation 17081983,18669648,19651622 CDC23 594 S Phosphorylation 17081983,18669648,19651622,20068231 CDC23 596 T Phosphorylation 16565220,18669648,19413330,18691976,19651622,16964243,18767875,20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9UJX2 578 T 22067460 HTP Phosphorylation Q9UJX2 578 T 18669648 HTP Phosphorylation Q9UJX2 267 Y 14657031 HTP Phosphorylation Q9UJX2 576 T 22067460 HTP Phosphorylation Q9UJX2 576 T 18669648 HTP Phosphorylation Q9UJX2 576 T 14657031 CDK1 HTP Phosphorylation Q9UJX2 587 S 22067460 HTP Phosphorylation Q9UJX2 587 S 18669648 HTP Phosphorylation Q9UJX2 587 S 17081983 HTP Phosphorylation Q9UJX2 559 T 18669648 HTP Phosphorylation Q9UJX2 559 T 14657031 HTP Phosphorylation Q9UJX2 559 T 14657031 LTP Phosphorylation Q9UJX2 590 T 22067460 HTP Phosphorylation Q9UJX2 590 T 18669648 HTP Phosphorylation Q9UJX2 590 T 16964243 HTP Phosphorylation Q9UJX2 590 T 17081983 HTP Phosphorylation Q9UJX2 590 T 16565220 HTP Phosphorylation Q9UJX2 582 S 22067460 HTP Phosphorylation Q9UJX2 582 S 18669648 HTP Phosphorylation Q9UJX2 582 S 16964243 HTP Phosphorylation Q9UJX2 582 S 17081983 HTP Phosphorylation Q9UJX2 582 S 15302935 HTP Phosphorylation Q9UJX2 556 T 22067460 HTP Phosphorylation Q9UJX2 556 T 18669648 HTP Phosphorylation Q9UJX2 556 T 14657031 HTP Phosphorylation Q9UJX2 556 T 14657031 CDK1 LTP Phosphorylation Q9UJX2 556 T 14657031 PLK1 LTP Phosphorylation Q9UJX2 588 S 22067460 HTP Phosphorylation Q9UJX2 588 S 18669648 HTP Phosphorylation Q9UJX2 572 S 22067460 HTP Phosphorylation Q9UJX2 572 S 18669648 HTP Phosphorylation Q9UJX2 570 S 22067460 HTP Phosphorylation Q9UJX2 570 S 18669648 HTP Phosphorylation Q9UJX2 558 T 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9UJX2 2 N-acetylalanine 22814378 Q9UJX2 273 Phosphotyrosine 14657031 Q9UJX2 467 N6-acetyllysine 19608861 Q9UJX2 562 Phosphothreonine 18669648;14657031 Q9UJX2 565 Phosphothreonine 14657031 Q9UJX2 578 Phosphoserine 19690332;23186163 Q9UJX2 582 Phosphothreonine 18669648;19690332 Q9UJX2 588 Phosphoserine 16964243;18669648;18691976;19690332;20068231;21406692;23186163;24275569 Q9UJX2 593 Phosphoserine 18669648;23186163 Q9UJX2 596 Phosphothreonine 16964243;18669648;19690332;20068231;21406692;23186163;24275569

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type CDC23 IPI00005822 K 467 AVGDVEaKMALVKL acetylation CDC23 IPI00005822 S 304 ENMDTFpSNLLYVR phosphorylation CDC23 IPI00005822 S 576 PFFLPApSLSANNT phosphorylation CDC23 IPI00005822 S 578 FLPASLpSANNTPT phosphorylation CDC23 IPI00005822 S 588 TPTRRVpSPLNLSS phosphorylation CDC23 IPI00005822 S 593 VSPLNLpSSVTP__ phosphorylation CDC23 IPI00005822 T 562 LRNQGEpTPTTEVP phosphorylation CDC23 IPI00005822 T 582 SLSANNpTPTRRVS phosphorylation CDC23 IPI00005822 T 596 LNLSSVpTP_____ phosphorylation CDC23 IPI00005822 Y 308 TFSNLLpYVRSMKS phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 8697 114242 - K Ubiquitination 21139048 8697 114242 - K Ubiquitination 21906983 8697 114242 - K Ubiquitination 22505724 8697 114242 - K Ubiquitination 21963094 8697 114242 - K Ubiquitination 23000965 8697 114242 - K Ubiquitination 25147182 8697 114242 - K Sumoylation 25391492

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID CDC23 Homo sapiens (Human) 137; 275; 461; 141; 447; 541; 91 "137,SLGPLEKGQVKNE; 275,NIRDIDKALSIFN; 461,AVGDVEKMALVKL; 141,LEKGQVKNEALRE; 447,NQLVEAKKCYWRA; 541,DTREEGKALLRQI; 91,KAYFDVKEYDRAA" 137:21906983; 275:21906983; 461:21906983; 141:21906983; 447:21906983; 541:21906983; 91:21906983 CDC23 Homo sapiens (Human) 97 "97,KAYFDVKEYDRAA" 97:21906983 CDC23 Homo sapiens (Human) 547; 143; 97; 281; 453; 147; 467 "547,DTREEGKALLRQI; 143,SLGPLEKGQVKNE; 97,KAYFDVKEYDRAA; 281,NIRDIDKALSIFN; 453,NQLVEAKKCYWRA; 147,LEKGQVKNEALRE; 467,AVGDVEKMALVKL" 547:21906983; 143:21906983; 97:21906983; 281:21906983; 453:21906983; 147:21906983; 467:21906983