PTM - CPLM Gene Name UniProt ID Type Position Code SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Acetylation 23 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Acetylation 37 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Acetylation 39 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Acetylation 45 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Acetylation 46 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Acetylation 48 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Sumoylation 7 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Sumoylation 25 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Ubiquitination 25 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Ubiquitination 37 K SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 Ubiquitination 48 K

PTM - dbPAF Gene Name UniProt ID Position Type Species SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 10 T Homo sapiens(Human) SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 2 S Homo sapiens(Human) SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 21 Y Homo sapiens(Human) SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 31 S Homo sapiens(Human) SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 32 S Homo sapiens(Human) SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 41 T Homo sapiens(Human) SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 76 T Homo sapiens(Human) SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43 P63165 9 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P63165 K23-ac Acetylation kkEGEYIkLkVIGQD P63165 K37-ac Acetylation DSsEIHFkVkMTTHL P63165 K37-m1 Methylation DSsEIHFkVkMTTHL P63165 K39-m1 Methylation sEIHFkVkMTTHLkk P63165 R63-m1 Methylation GVPMNSLrFLFEGQr P63165 R70-m1 Methylation rFLFEGQrIADNHtP P63165 S2-p Phosphorylation ______MsDQEAkPs P63165 S9-p Phosphorylation sDQEAkPstEDLGDk P63165 T10-p Phosphorylation DQEAkPstEDLGDkk P63165 S32-p Phosphorylation kVIGQDSsEIHFkVk P63165 T76-p Phosphorylation QrIADNHtPkELGME P63165 K7-sm Sumoylation _MsDQEAkPstEDLG P63165 K16-sm Sumoylation stEDLGDkkEGEYIk P63165 K17-sm Sumoylation tEDLGDkkEGEYIkL P63165 K23-sm Sumoylation kkEGEYIkLkVIGQD P63165 K25-sm Sumoylation EGEYIkLkVIGQDSs P63165 K37-sm Sumoylation DSsEIHFkVkMTTHL P63165 K39-sm Sumoylation sEIHFkVkMTTHLkk P63165 K45-sm Sumoylation VkMTTHLkkLkESYC P63165 K46-sm Sumoylation kMTTHLkkLkESYCQ P63165 K48-sm Sumoylation TTHLkkLkESYCQRQ P63165 K7-ub Ubiquitination _MsDQEAkPstEDLG P63165 K17-ub Ubiquitination tEDLGDkkEGEYIkL P63165 K23-ub Ubiquitination kkEGEYIkLkVIGQD P63165 K25-ub Ubiquitination EGEYIkLkVIGQDSs P63165 K37-ub Ubiquitination DSsEIHFkVkMTTHL P63165 K39-ub Ubiquitination sEIHFkVkMTTHLkk P63165 K48-ub Ubiquitination TTHLkkLkESYCQRQ P63165 K78-ub Ubiquitination IADNHtPkELGMEEE

PTM - dbPTM UniProt ID Position Code Type Resources References P63165 2 S Acetylation Swiss-Prot 1010711 20388717 P63165 2 S Phosphorylation Swiss-Prot 1010711 17081983;18691976;18707152;19413330 P63165 9 S Phosphorylation Swiss-Prot 1010711 19369195 P63165 97 G Isopeptide bond Swiss-Prot 1010711 - P63165 7 K Sumoylation Swiss-Prot 1010711 20388717 P63165 25 K Sumoylation Swiss-Prot 1010711 20388717 P63165 2 S Phosphorylation PhosphoELM.10011 17081983 P63165 16 K Sumoylation Phosphositeplus.1010730 16194093 P63165 17 K Sumoylation Phosphositeplus.1010730 19721078 P63165 25 K Sumoylation Phosphositeplus.1010730 20388717 P63165 37 K Sumoylation Phosphositeplus.1010730 16194093 P63165 39 K Sumoylation Phosphositeplus.1010730 16194093;21474068 P63165 46 K Sumoylation Phosphositeplus.1010730 16194093 P63165 7 K Sumoylation Phosphositeplus.1010730 20388717;20797634 P63165 25 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P63165 37 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P63165 48 K Ubiquitylation Phosphositeplus.1010730 21890473 P63165 23 K Acetylation Phosphositeplus.1010730 19608861 P63165 2 S Phosphorylation Phosphositeplus.1010730 18691976;18707152;19362540;19664995;19691289;20068231;20164059;20363803;20797634;21406692;22115753 P63165 9 S Phosphorylation Phosphositeplus.1010730 19664995;20068231 P63165 10 T Phosphorylation Phosphositeplus.1010730 19664995 P63165 23 K Acetylation HPRD 9.0 19608861 P63165 2 S Phosphorylation HPRD 9.0 18707152,19413330,18691976,19007248,19651622,19691289,20068231 P63165 2 S Phosphorylation HPRD 9.0 17081983,19413330,18691976,19007248,19651622,19691289,20068231 P63165 9 S Phosphorylation HPRD 9.0 17081983,18691976,19651622,20068231 P63165 10 T Phosphorylation HPRD 9.0 17081983,19651622,20068231 P63165 2 S Phosphorylation SysPTM 1.1 17081983;18691976 P63165 9 S Phosphorylation SysPTM 1.1 17081983 P63165 10 T Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References SUMO1 10 T Phosphorylation 17081983,19651622,20068231 SUMO1 2 S Phosphorylation 17081983,19413330,18691976,19007248,19651622,19691289,20068231 SUMO1 2 S Phosphorylation 18707152,19413330,18691976,19007248,19651622,19691289,20068231 SUMO1 23 K Acetylation 19608861 SUMO1 9 S Phosphorylation 17081983,18691976,19651622,20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P63165 2 S 17081983 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P63165 2 N-acetylserine 19413330;20068231;21406692;20388717 P63165 2 Phosphoserine 17081983;18691976;20068231;21406692;23186163;18707152 P63165 9 Phosphoserine 20068231 P63165 32 Phosphoserine 23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type SUMO1 IPI00303105 K 23 KEGEYIaKLKVIGQ acetylation SUMO1 IPI00303105 S 2 _____MpSDQEAKP phosphorylation SUMO1 IPI00303105 S 9 DQEAKPpSTEDLGD phosphorylation SUMO1 IPI00303105 T 10 QEAKPSpTEDLGDK phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 7341 113188 - K Ubiquitination 18781797 7341 113188 - K Ubiquitination 21987572 7341 113188 - K Sumoylation 21693764 7341 113188 - K Ubiquitination 21139048 7341 113188 - K Ubiquitination 21890473 7341 113188 - K Ubiquitination 21906983 7341 113188 - K Sumoylation 19471022 7341 113188 - K Sumoylation 21252943 7341 113188 - K Sumoylation 15576338 7341 113188 - K Sumoylation 15175327 7341 113188 - K Ubiquitination 22505724 7341 113188 - K Ubiquitination 23000965 7341 113188 - K Ubiquitination 24654937 7341 113188 - K Sumoylation 24654937 7341 113188 - K Sumoylation 25027693 7341 113188 - K Ubiquitination 25147182 7341 113188 - K Sumoylation 25391492 7341 113188 - K Sumoylation 17938407

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID SUMO1 Homo sapiens (Human) 9 "9,THLKKLKESYCQR" 9:21890473 SUMO1 Homo sapiens (Human) 23 "23,THLKKLKESYCQR" 23:21890473 SUMO1 Homo sapiens (Human) 48; 37; 25 "48,THLKKLKESYCQR; 37,SSEIHFKVKMTTH; 25,GEYIKLKVIGQDS" "48:21890473; 37:21890473; 25:21906983,21890473" SUMO1 Homo sapiens (Human) 48; 37; 25 "48,THLKKLKESYCQR; 37,SSEIHFKVKMTTH; 25,GEYIKLKVIGQDS" "48:21890473; 37:21906983,21890473; 25:21906983,21890473" SUMO1 Homo sapiens (Human) 48; 37; 25 "48,THLKKLKESYCQR; 37,SSEIHFKVKMTTH; 25,GEYIKLKVIGQDS" "48:21890473; 37:21906983,21890473; 25:21906983,21890473" SUMO1 Homo sapiens (Human) 25 "25,GEYIKLKVIGQDS" 25:21890473 SUMO1 Homo sapiens (Human) 48; 37; 25 "48,THLKKLKESYCQR; 37,SSEIHFKVKMTTH; 25,GEYIKLKVIGQDS" "48:21890473; 37:21139048,21890473,21906983; 25:21139048,21890473,21906983"