PTM - CPLM Gene Name UniProt ID Type Position Code PLCG1; PLC1 P19174 Ubiquitination 389 K PLCG1; PLC1 P19174 Ubiquitination 431 K PLCG1; PLC1 P19174 Ubiquitination 941 K PLCG1; PLC1 P19174 Ubiquitination 178 K PLCG1; PLC1 P19174 Ubiquitination 226 K PLCG1; PLC1 P19174 Ubiquitination 440 K PLCG1; PLC1 P19174 Ubiquitination 1079 K PLCG1; PLC1 P19174 Ubiquitination 1193 K PLCG1; PLC1 P19174 Ubiquitination 987 K PLCG1; PLC1 P19174 Ubiquitination 890 K PLCG1; PLC1 P19174 Ubiquitination 175 K PLCG1; PLC1 P19174 Ubiquitination 465 K PLCG1; PLC1 P19174 Ubiquitination 159 K PLCG1; PLC1 P19174 Ubiquitination 763 K PLCG1; PLC1 P19174 Ubiquitination 666 K PLCG1; PLC1 P19174 Ubiquitination 430 K PLCG1; PLC1 P19174 Ubiquitination 793 K PLCG1; PLC1 P19174 Ubiquitination 797 K PLCG1; PLC1 P19174 Ubiquitination 456 K PLCG1; PLC1 P19174 Ubiquitination 554 K PLCG1; PLC1 P19174 Ubiquitination 54 K

PTM - dbPAF Gene Name UniProt ID Position Type Species PLCG1; PLC1 P19174 1003 Y Homo sapiens(Human) PLCG1; PLC1 P19174 1221 S Homo sapiens(Human) PLCG1; PLC1 P19174 1224 S Homo sapiens(Human) PLCG1; PLC1 P19174 1226 T Homo sapiens(Human) PLCG1; PLC1 P19174 1227 S Homo sapiens(Human) PLCG1; PLC1 P19174 1233 S Homo sapiens(Human) PLCG1; PLC1 P19174 1236 S Homo sapiens(Human) PLCG1; PLC1 P19174 1248 S Homo sapiens(Human) PLCG1; PLC1 P19174 1251 S Homo sapiens(Human) PLCG1; PLC1 P19174 1253 Y Homo sapiens(Human) PLCG1; PLC1 P19174 1263 S Homo sapiens(Human) PLCG1; PLC1 P19174 1273 S Homo sapiens(Human) PLCG1; PLC1 P19174 186 Y Homo sapiens(Human) PLCG1; PLC1 P19174 210 Y Homo sapiens(Human) PLCG1; PLC1 P19174 219 S Homo sapiens(Human) PLCG1; PLC1 P19174 236 S Homo sapiens(Human) PLCG1; PLC1 P19174 237 T Homo sapiens(Human) PLCG1; PLC1 P19174 379 Y Homo sapiens(Human) PLCG1; PLC1 P19174 412 S Homo sapiens(Human) PLCG1; PLC1 P19174 428 Y Homo sapiens(Human) PLCG1; PLC1 P19174 451 S Homo sapiens(Human) PLCG1; PLC1 P19174 470 S Homo sapiens(Human) PLCG1; PLC1 P19174 472 Y Homo sapiens(Human) PLCG1; PLC1 P19174 477 T Homo sapiens(Human) PLCG1; PLC1 P19174 481 Y Homo sapiens(Human) PLCG1; PLC1 P19174 496 Y Homo sapiens(Human) PLCG1; PLC1 P19174 506 Y Homo sapiens(Human) PLCG1; PLC1 P19174 509 Y Homo sapiens(Human) PLCG1; PLC1 P19174 518 Y Homo sapiens(Human) PLCG1; PLC1 P19174 519 Y Homo sapiens(Human) PLCG1; PLC1 P19174 520 S Homo sapiens(Human) PLCG1; PLC1 P19174 523 T Homo sapiens(Human) PLCG1; PLC1 P19174 524 S Homo sapiens(Human) PLCG1; PLC1 P19174 525 S Homo sapiens(Human) PLCG1; PLC1 P19174 539 S Homo sapiens(Human) PLCG1; PLC1 P19174 542 T Homo sapiens(Human) PLCG1; PLC1 P19174 6 S Homo sapiens(Human) PLCG1; PLC1 P19174 669 Y Homo sapiens(Human) PLCG1; PLC1 P19174 702 Y Homo sapiens(Human) PLCG1; PLC1 P19174 766 T Homo sapiens(Human) PLCG1; PLC1 P19174 771 Y Homo sapiens(Human) PLCG1; PLC1 P19174 775 Y Homo sapiens(Human) PLCG1; PLC1 P19174 783 Y Homo sapiens(Human) PLCG1; PLC1 P19174 791 T Homo sapiens(Human) PLCG1; PLC1 P19174 833 Y Homo sapiens(Human) PLCG1; PLC1 P19174 93 Y Homo sapiens(Human) PLCG1; PLC1 P19174 954 S Homo sapiens(Human) PLCG1; PLC1 P19174 959 Y Homo sapiens(Human) PLCG1; PLC1 P19174 977 Y Homo sapiens(Human) PLCG1; PLC1 P19174 981 S Homo sapiens(Human) PLCG1; PLC1 P19174 982 S Homo sapiens(Human) PLCG1; PLC1 P19174 991 Y Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P19174 R1231-m1 Methylation sGtsLRErGsDAsGQ P19174 R1243-m1 Methylation sGQLFHGrAREGsFE P19174 R1283-m1 Methylation RRAPRRTrVNGDNRL P19174 S6-p Phosphorylation __MAGAAsPCANGCG P19174 Y93-p Phosphorylation TSRDFDRyQEDPAFR P19174 Y186-p Phosphorylation NMLSQVNyRVPNMRF P19174 Y210-p Phosphorylation QRSGDITyGQFAQLY P19174 S219-p Phosphorylation QFAQLYRsLMYSAQk P19174 S236-p Phosphorylation DLPFLEAstLRAGER P19174 T237-p Phosphorylation LPFLEAstLRAGERP P19174 Y379-p Phosphorylation PDGMPVIyHGHTLTT P19174 S412-p Phosphorylation SEYPVILsIEDHCSI P19174 Y428-p Phosphorylation QQRNMAQyFKkVLGD P19174 S451-p Phosphorylation ISADGLPsPNQLKRK P19174 S470-p Phosphorylation HKKLAEGsAyEEVPt P19174 Y472-p Phosphorylation KLAEGsAyEEVPtSM P19174 T477-p Phosphorylation sAyEEVPtSMMySEN P19174 Y481-p Phosphorylation EVPtSMMySENDISN P19174 Y496-p Phosphorylation SIKNGILyLEDPVNH P19174 Y506-p Phosphorylation DPVNHEWyPHyFVLT P19174 Y509-p Phosphorylation NHEWyPHyFVLTSSK P19174 Y518-p Phosphorylation VLTSSKIyYSEETSs P19174 S525-p Phosphorylation yYSEETSsDQGNEDE P19174 S539-p Phosphorylation EEEPKEVsSStELHS P19174 T542-p Phosphorylation PKEVsSStELHSNEK P19174 S582-p Phosphorylation ETGAPDGsFLVRESE P19174 Y669-p Phosphorylation AHESKEWyHASLTRA P19174 Y702-p Phosphorylation KRNEPNSyAISFRAE P19174 T766-p Phosphorylation EALEKIGtAEPdyGA P19174 Y771-p Phosphorylation IGtAEPdyGALyEGR P19174 Y775-p Phosphorylation EPdyGALyEGRNPGF P19174 Y783-p Phosphorylation EGRNPGFyVEANPMP P19174 T791-p Phosphorylation VEANPMPtFKCAVKA P19174 S815-p Phosphorylation DELTFIKsAIIQNVE P19174 Y833-p Phosphorylation GGWWRGDyGGKKQLW P19174 S954-p Phosphorylation KKIALELsELVVyCR P19174 Y959-p Phosphorylation ELsELVVyCRPVPFD P19174 Y977-p Phosphorylation IGTERACyRDMssFP P19174 S981-p Phosphorylation RACyRDMssFPETKA P19174 S982-p Phosphorylation ACyRDMssFPETKAE P19174 Y991-p Phosphorylation PETKAEKyVNKAKGK P19174 Y1003-p Phosphorylation KGKKFLQyNRLQLSR P19174 S1221-p Phosphorylation AKENGDLsPFsGtsL P19174 S1224-p Phosphorylation NGDLsPFsGtsLREr P19174 T1226-p Phosphorylation DLsPFsGtsLRErGs P19174 S1227-p Phosphorylation LsPFsGtsLRErGsD P19174 S1233-p Phosphorylation tsLRErGsDAsGQLF P19174 S1236-p Phosphorylation RErGsDAsGQLFHGr P19174 S1248-p Phosphorylation HGrAREGsFEsRyQQ P19174 S1251-p Phosphorylation AREGsFEsRyQQPFE P19174 Y1253-p Phosphorylation EGsFEsRyQQPFEDF P19174 S1263-p Phosphorylation PFEDFRIsQEHLADH P19174 S1273-p Phosphorylation HLADHFDsRERRAPR P19174 K54-ub Ubiquitination ERKTFQVkLETRQIT P19174 K178-ub Ubiquitination RISAKDLkNMLSQVN P19174 K226-ub Ubiquitination sLMYSAQkTMDLPFL P19174 K389-ub Ubiquitination HTLTTKIkFSDVLHT P19174 K431-ub Ubiquitination NMAQyFKkVLGDTLL P19174 K440-ub Ubiquitination LGDTLLTkPVEISAD P19174 K941-ub Ubiquitination DARLTEGkIMERRKK P19174 K1079-ub Ubiquitination EAFDPFDkSSLRGLE P19174 K1193-ub Ubiquitination GYRAVPLkNNYSEDL

PTM - dbPTM UniProt ID Position Code Type Resources References P19174 2 A Acetylation Swiss-Prot 1010711 19413330 P19174 6 S Phosphorylation Swiss-Prot 1010711 19413330 P19174 525 S Phosphorylation Swiss-Prot 1010711 19413330 P19174 771 Y Phosphorylation Swiss-Prot 1010711 8657103;15592455;17081983;17389395;19534553;19690332 P19174 775 Y Phosphorylation Swiss-Prot 1010711 15592455;15951569;19534553;19690332 P19174 783 Y Phosphorylation Swiss-Prot 1010711 8657103;11564877;15215251;15951569;17389395;19534553;19690332 P19174 977 Y Phosphorylation Swiss-Prot 1010711 18083107 P19174 1221 S Phosphorylation Swiss-Prot 1010711 18669648;19690332 P19174 1253 Y Phosphorylation Swiss-Prot 1010711 17081983;17389395;19690332 P19174 1263 S Phosphorylation Swiss-Prot 1010711 18220336;19690332 P19174 1221 S Phosphorylation PhosphoELM.10011 18669648 P19174 1233 S Phosphorylation PhosphoELM.10011 18220336 P19174 1263 S Phosphorylation PhosphoELM.10011 18220336 P19174 1248 S Phosphorylation PhosphoELM.10011 1370476;1370476 P19174 472 Y Phosphorylation PhosphoELM.10011 12601080;7991458;1689310 P19174 775 Y Phosphorylation PhosphoELM.10011 15592455 P19174 977 Y Phosphorylation PhosphoELM.10011 18083107 P19174 1253 Y Phosphorylation PhosphoELM.10011 17081983;15144186;12601080;1708307;1689310 P19174 771 Y Phosphorylation PhosphoELM.10011 16038803;15592455;15144186;12601080;1708307;1689310;8657103 P19174 783 Y Phosphorylation PhosphoELM.10011 16038803;16126727;12601080;1708307;1689310;8657103 P19174 770 D Caspase Phosphositeplus.1010730 18724940 P19174 178 K Ubiquitylation Phosphositeplus.1010730 21906983 P19174 226 K Ubiquitylation Phosphositeplus.1010730 21906983 P19174 389 K Ubiquitylation Phosphositeplus.1010730 21890473 P19174 431 K Ubiquitylation Phosphositeplus.1010730 21890473 P19174 440 K Ubiquitylation Phosphositeplus.1010730 21906983 P19174 941 K Ubiquitylation Phosphositeplus.1010730 21890473 P19174 1221 S Phosphorylation Phosphositeplus.1010730 18669648;19664994;19690332;20068231 P19174 1233 S Phosphorylation Phosphositeplus.1010730 18220336;19276368;20363803 P19174 1248 S Phosphorylation Phosphositeplus.1010730 1370476;16525023;20068231;20736484;21406692;21840932;22115753 P19174 1263 S Phosphorylation Phosphositeplus.1010730 17016520;18077418;18220336;19276368;19362540;19690332;20363803;20393185;22115753 P19174 1253 Y Phosphorylation Phosphositeplus.1010730 12601080;15316024;15570572;16038803;17016520;17081983;17389395;17646646;17870612;18707149;19690332;20068231;20736484;22157759 P19174 472 Y Phosphorylation Phosphositeplus.1010730 12601080;19901323;20007894 P19174 481 Y Phosphorylation Phosphositeplus.1010730 18083107;19901323 P19174 496 Y Phosphorylation Phosphositeplus.1010730 22115753 P19174 506 Y Phosphorylation Phosphositeplus.1010730 19658100;22115753 P19174 771 Y Phosphorylation Phosphositeplus.1010730 12601080;15316024;15592455;15951569;16038803;16493040;16525023;17016520;17389395;17646646;17870612;18083107;18180459;19534553;19605366;19690332;20007894;20562096;20736484;22115753;8657103 P19174 775 Y Phosphorylation Phosphositeplus.1010730 15592455;15951569;16493040;16497976;19534553;19605366;19690332;20068231;20736484 P19174 783 Y Phosphorylation Phosphositeplus.1010730 11839772;12029091;12601080;12730118;12952950;14523024;14568990;15026417;15316024;15509800;15558056;15623534;15696170;15708852;15951569;15975899;16038803;16081816;16126727;16497976;16525023;17016520;17148460;17188005;17250586;17303569;17360941;17372230;17389395;17561374;17998336;18083107;18180459;18707149;18936167;19534553;19605366;19658100;19690332;20007894;20068231;20562096;20736484;21278788;21659545;21760611;21840932;22115753;8657103 P19174 977 Y Phosphorylation Phosphositeplus.1010730 18083107;19658100;19901323;20736484;21253578;22115753 P19174 6 S Phosphorylation HPRD 9.0 19413330 P19174 525 S Phosphorylation HPRD 9.0 19413330 P19174 1221 S Phosphorylation HPRD 9.0 18669648,19664994,20068231 P19174 1233 S Phosphorylation HPRD 9.0 20068231 P19174 1263 S Phosphorylation HPRD 9.0 18077418 P19174 791 T Phosphorylation HPRD 9.0 20068231 P19174 481 Y Phosphorylation HPRD 9.0 19901323 P19174 775 Y Phosphorylation HPRD 9.0 15951569,16497976,20068231 P19174 783 Y Phosphorylation HPRD 9.0 8885868,8657103,1689310,7682059,17389395,16497976,17016520,20068231 P19174 977 Y Phosphorylation HPRD 9.0 18083107,19901323 P19174 1253 Y Phosphorylation HPRD 9.0 8885868,7682059,15144186,17389395,17016520,18707149,20068231 P19174 1248 S Phosphorylation (PRKD1) HPRD 9.0 8885868,19651622,20068231 P19174 472 Y Phosphorylation (EGFR) HPRD 9.0 12601080,1689310,19901323 P19174 771 Y Phosphorylation (EGFR) HPRD 9.0 8657103,1689310,7682059,15144186,17389395,16497976,17016520,20068231 P19174 783 Y Phosphorylation (EGFR) HPRD 9.0 8885868,8657103,1689310,7682059,17389395,16497976,17016520,20068231 P19174 771 Y Phosphorylation (HCK) HPRD 9.0 8657103,1689310,7682059,15144186,17389395,16497976,17016520,20068231 P19174 783 Y Phosphorylation (HCK) HPRD 9.0 8885868,8657103,1689310,7682059,17389395,16497976,17016520,20068231 P19174 1253 Y Phosphorylation (HCK) HPRD 9.0 8885868,7682059,15144186,17389395,17016520,18707149,20068231 P19174 771 Y Phosphorylation (LYN) HPRD 9.0 8657103,1689310,7682059,15144186,17389395,16497976,17016520,20068231 P19174 783 Y Phosphorylation (LYN) HPRD 9.0 8885868,8657103,1689310,7682059,17389395,16497976,17016520,20068231 P19174 783 Y Phosphorylation (RET) HPRD 9.0 8885868,8657103,1689310,7682059,17389395,16497976,17016520,20068231 P19174 783 Y Phosphorylation (SRC) HPRD 9.0 8885868,8657103,1689310,7682059,17389395,16497976,17016520,20068231 P19174 771 Y Phosphorylation (SYK) HPRD 9.0 8657103,1689310,7682059,15144186,17389395,16497976,17016520,20068231 P19174 783 Y Phosphorylation (SYK) HPRD 9.0 8885868,8657103,1689310,7682059,17389395,16497976,17016520,20068231 P19174 1221 S Phosphorylation SysPTM 1.1 18669648 P19174 771 Y Phosphorylation SysPTM 1.1 17081983 P19174 783 Y Phosphorylation SysPTM 1.1 18707149 P19174 1253 Y Phosphorylation SysPTM 1.1 17081983;18707149

PTM - HPRD Symbol Position Code Type References PLCG1 1221 S Phosphorylation 18669648,19664994,20068231 PLCG1 1233 S Phosphorylation 20068231 PLCG1 1248 S Phosphorylation 8885868,19651622,20068231 PLCG1 1253 Y Phosphorylation 8885868,7682059,15144186,17389395,17016520,18707149,20068231 PLCG1 1263 S Phosphorylation 18077418 PLCG1 472 Y Phosphorylation 12601080,1689310,19901323 PLCG1 481 Y Phosphorylation 19901323 PLCG1 525 S Phosphorylation 19413330 PLCG1 6 S Phosphorylation 19413330 PLCG1 771 Y Phosphorylation 8657103,1689310,7682059,15144186,17389395,16497976,17016520,20068231 PLCG1 775 Y Phosphorylation 15951569,16497976,20068231 PLCG1 783 Y Phosphorylation 8885868,8657103,1689310,7682059,17389395,16497976,17016520,20068231 PLCG1 791 T Phosphorylation 20068231 PLCG1 977 Y Phosphorylation 18083107,19901323

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P19174 977 Y 18083107 HTP Phosphorylation P19174 1248 S 1370476 PKA_group LTP Phosphorylation P19174 1248 S 1370476 PKC_alpha LTP Phosphorylation P19174 771 Y 16038803 LTP Phosphorylation P19174 771 Y 15592455 HTP Phosphorylation P19174 771 Y 15144186 HTP Phosphorylation P19174 771 Y 12601080 LTP Phosphorylation P19174 771 Y 1708307 LTP Phosphorylation P19174 771 Y 1689310 EGFR LTP Phosphorylation P19174 771 Y 8657103 SYK LTP Phosphorylation P19174 1221 S 18669648 HTP Phosphorylation P19174 775 Y 15592455 HTP Phosphorylation P19174 783 Y 16038803 LTP Phosphorylation P19174 783 Y 16126727 LTP Phosphorylation P19174 783 Y 12601080 LTP Phosphorylation P19174 783 Y 1708307 LTP Phosphorylation P19174 783 Y 1689310 EGFR LTP Phosphorylation P19174 783 Y 8657103 SYK LTP Phosphorylation P19174 1233 S 18220336 HTP Phosphorylation P19174 472 Y 12601080 LTP Phosphorylation P19174 472 Y 7991458 LTP Phosphorylation P19174 472 Y 1689310 LTP Phosphorylation P19174 1263 S 18220336 HTP Phosphorylation P19174 1253 Y 17081983 HTP Phosphorylation P19174 1253 Y 15144186 HTP Phosphorylation P19174 1253 Y 12601080 LTP Phosphorylation P19174 1253 Y 1708307 LTP Phosphorylation P19174 1253 Y 1689310 EGFR LTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P19174 2 N-acetylalanine 19413330 P19174 771 Phosphotyrosine; by SYK 15144186;19690332;20494825;8657103 P19174 775 Phosphotyrosine 19690332 P19174 783 Phosphotyrosine; by ITK, SYK and TXK 19690332;11564877;15215251;8657103 P19174 1221 Phosphoserine 18669648;19690332;20068231;23186163 P19174 1227 Phosphoserine 23186163 P19174 1233 Phosphoserine 23186163 P19174 1248 Phosphoserine 1370476 P19174 1253 Phosphotyrosine 15144186;20068231 P19174 1263 Phosphoserine 23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type PLCG1 IPI00016736 Y 1253 GSFESRpYQQPFED phosphorylation PLCG1 IPI00016736 Y 771 GTAEPDpYGALYEG phosphorylation PLCG1 IPI00383849 S 1222 QENGDLpSPFSGTS phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 5335 111351 - K Ubiquitination 18671761 5335 111351 - K Ubiquitination 17372230 5335 111351 - K Ubiquitination 21139048 5335 111351 - K Ubiquitination 21890473 5335 111351 - K Ubiquitination 21906983 5335 111351 - K Ubiquitination 23000965 5335 111351 - K Ubiquitination 24816145

PTM - PhosphoBase Gene Name PB Accession PROT ID Serine Threonine Tyrosine Peptide PLCG1;PLC1 A054 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1 (PLC-gamma-1) (phospholipase C-gamma-1) - - 771 [A054-A], 783 [A054-B], 1253 [A054-C] A054-C - FEARYQQPF;!

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID PLCG1 Homo sapiens (Human) 941; 431; 389 "941,ARLTEGKIMERRK; 431,MAQYFKKVLGDTL; 389,TLTTKIKFSDVLH" 941:21890473; 431:21890473; 389:21890473 PLCG1 Homo sapiens (Human) 226 "226,ARLTEGKIMERRK" 226:21890473 PLCG1 Homo sapiens (Human) 941; 431; 389 "941,ARLTEGKIMERRK; 431,MAQYFKKVLGDTL; 389,TLTTKIKFSDVLH" 941:21890473; 431:21890473; 389:21890473 PLCG1 Homo sapiens (Human) 440; 941; 431; 178; 226; 389 "440,GDTLLTKPVEISA; 941,ARLTEGKIMERRK; 431,MAQYFKKVLGDTL; 178,ISAKDLKNMLSQV; 226,LMYSAQKTMDLPF; 389,TLTTKIKFSDVLH" 440:21906983; 941:21890473; 431:21890473; 178:21906983; 226:21906983; 389:21890473 PLCG1 Homo sapiens (Human) 941; 511; 431; 1063; 389; 553 "941,ARLTEGKIMERRK; 511,TLTTKIKFSDVLH; 431,MAQYFKKVLGDTL; 1063,ARLTEGKIMERRK; 389,TLTTKIKFSDVLH; 553,MAQYFKKVLGDTL" 440:21906983; 941:21890473; 431:21890473; 178:21906983; 226:21906983; 389:21890473 PLCG1 Homo sapiens (Human) 941; 431; 389 "941,ARLTEGKIMERRK; 431,MAQYFKKVLGDTL; 389,TLTTKIKFSDVLH" 941:21890473; 431:21890473; 389:21890473 PLCG1 Homo sapiens (Human) 941; 431; 389 "941,ARLTEGKIMERRK; 431,MAQYFKKVLGDTL; 389,TLTTKIKFSDVLH" 941:21890473; 431:21890473; 389:21890473