PTM - CPLM Gene Name UniProt ID Type Position Code USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1627 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 218 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 315 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 329 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 424 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 486 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 571 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 794 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1045 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1153 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1370 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1632 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1637 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1669 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1712 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1722 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1798 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1811 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1862 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 2301 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 2360 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1899 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1453 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1935 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1435 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1445 K USP9X; DFFRX; FAM; USP9 Q93008 Ubiquitination 1446 K

PTM - dbPAF Gene Name UniProt ID Position Type Species USP9X; DFFRX; FAM; USP9 Q93008 1232 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 1591 T Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 1593 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 1600 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 1609 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 1620 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 199 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2402 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2438 T Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2439 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2443 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2448 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2452 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2556 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2559 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2563 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 2567 T Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 313 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 367 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 368 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 403 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 409 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 588 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 590 T Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 593 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 701 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 702 S Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 868 Y Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 930 T Homo sapiens(Human) USP9X; DFFRX; FAM; USP9 Q93008 984 T Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q93008 K303-ac Acetylation KEAKNEAkNDALsMI Q93008 K315-ac Acetylation sMIIKsLkNLASRVP Q93008 K1722-ac Acetylation GGSFADQkICQGCPH Q93008 R560-m1 Methylation DRFIEELrTNDKWVI Q93008 R844-m1 Methylation KDSVNCArQEAVRMV Q93008 R2366-m1 Methylation LkGIPDDrDGLFDTI Q93008 S199-p Phosphorylation PCESVSSsVQLPEDE Q93008 S308-p Phosphorylation EAkNDALsMIIKsLk Q93008 S313-p Phosphorylation ALsMIIKsLkNLASR Q93008 Y367-p Phosphorylation KVISSVSyyTHRHGN Q93008 Y368-p Phosphorylation VISSVSyyTHRHGNP Q93008 S403-p Phosphorylation LSIVLRDsLHQPQyV Q93008 Y409-p Phosphorylation DsLHQPQyVEKLEKI Q93008 S588-p Phosphorylation GEAPQNLsQtQRsPH Q93008 T590-p Phosphorylation APQNLsQtQRsPHVF Q93008 S593-p Phosphorylation NLsQtQRsPHVFYRH Q93008 Y701-p Phosphorylation REACFKWysKLMGDE Q93008 S702-p Phosphorylation EACFKWysKLMGDEP Q93008 Y797-p Phosphorylation IDLLkEIyTNLGPRL Q93008 Y868-p Phosphorylation INECDSDyHEERTIL Q93008 S887-p Phosphorylation AFRGKHLsFVVRFPN Q93008 T930-p Phosphorylation IKANVAHtKIELFVG Q93008 T984-p Phosphorylation DSSSDSStGSPGNHG Q93008 Y1148-p Phosphorylation METRRGAyLNALkIA Q93008 Y1232-p Phosphorylation ISDEASRyMPDICVI Q93008 Y1375-p Phosphorylation RAkHSGDyFTLLRHL Q93008 S1580-p Phosphorylation QQLYMIPsIRNGILA Q93008 T1591-p Phosphorylation GILAIEGtGsDVDDD Q93008 S1593-p Phosphorylation LAIEGtGsDVDDDMs Q93008 S1600-p Phosphorylation sDVDDDMsGDEKQDN Q93008 S1609-p Phosphorylation DEKQDNEsNVDPRDD Q93008 Y1620-p Phosphorylation PRDDVFGyPQQFEDk Q93008 T2438-p Phosphorylation YTGNPQYtyNNWsPP Q93008 Y2439-p Phosphorylation TGNPQYtyNNWsPPV Q93008 S2443-p Phosphorylation QYtyNNWsPPVQsNE Q93008 S2448-p Phosphorylation NWsPPVQsNETSNGY Q93008 Y2556-p Phosphorylation GQRAQENyEGsEEVs Q93008 S2559-p Phosphorylation AQENyEGsEEVsPPQ Q93008 S2563-p Phosphorylation yEGsEEVsPPQtKDQ Q93008 T2567-p Phosphorylation EEVsPPQtKDQ____ Q93008 K218-ub Ubiquitination SPDPRSPkGWLVDLL Q93008 K315-ub Ubiquitination sMIIKsLkNLASRVP Q93008 K329-ub Ubiquitination PGQEETVkNLEIFRL Q93008 K424-ub Ubiquitination LRFVIKEkALTLQDL Q93008 K486-ub Ubiquitination ASKKQREkLLELIRR Q93008 K571-ub Ubiquitination KWVIPALkQIREICS Q93008 K794-ub Ubiquitination SRAIDLLkEIyTNLG Q93008 K1045-ub Ubiquitination DGARVLMkLMPPDST Q93008 K1066-ub Ubiquitination AICLDHAkLGESSLS Q93008 K1153-ub Ubiquitination GAyLNALkIAKLLLT Q93008 K1370-ub Ubiquitination STARERAkHSGDyFT Q93008 K1627-ub Ubiquitination yPQQFEDkPALSkTE Q93008 K1632-ub Ubiquitination EDkPALSkTEDRkEY Q93008 K1637-ub Ubiquitination LSkTEDRkEYNIGVL Q93008 K1669-ub Ubiquitination YVPRGFWkQFRLWGE Q93008 K1712-ub Ubiquitination GHPAMLSkVLGGSFA Q93008 K1722-ub Ubiquitination GGSFADQkICQGCPH Q93008 K1798-ub Ubiquitination PVLAIQLkRFDYDWE Q93008 K1811-ub Ubiquitination WERECAIkFNDYFEF Q93008 K1862-ub Ubiquitination SETAGSTkYRLVGVL Q93008 K1899-ub Ubiquitination GERNRWYkFDDGDVT Q93008 K1935-ub Ubiquitination EVFDHMMkRMSYRRQ Q93008 K2219-ub Ubiquitination EGPGPPIkYQYAELG Q93008 K2301-ub Ubiquitination SNSEETVkLLRFCCW Q93008 K2360-ub Ubiquitination HRIHNALkGIPDDrD

PTM - dbPTM UniProt ID Position Code Type Resources References Q93008 590 T Phosphorylation Swiss-Prot 1010711 18691976 Q93008 1600 S Phosphorylation Swiss-Prot 1010711 17081983;18669648;18691976;19690332 Q93008 2443 S Phosphorylation Swiss-Prot 1010711 17693683;18669648 Q93008 2556 Y Phosphorylation Swiss-Prot 1010711 15592455 Q93008 2563 S Phosphorylation Swiss-Prot 1010711 16964243;18669648;18691976;19413330 Q93008 1593 S Phosphorylation PhosphoELM.10011 18669648;17081983 Q93008 1609 S Phosphorylation PhosphoELM.10011 18669648 Q93008 2443 S Phosphorylation PhosphoELM.10011 18669648 Q93008 2563 S Phosphorylation PhosphoELM.10011 18669648;16964243 Q93008 2556 Y Phosphorylation PhosphoELM.10011 15592455 Q93008 1045 K Ubiquitylation Phosphositeplus.1010730 21890473 Q93008 1370 K Ubiquitylation Phosphositeplus.1010730 21890473 Q93008 1627 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q93008 1632 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q93008 1712 K Ubiquitylation Phosphositeplus.1010730 21890473 Q93008 1798 K Ubiquitylation Phosphositeplus.1010730 21890473 Q93008 1862 K Ubiquitylation Phosphositeplus.1010730 21890473 Q93008 218 K Ubiquitylation Phosphositeplus.1010730 21890473 Q93008 2301 K Ubiquitylation Phosphositeplus.1010730 21890473 Q93008 329 K Ubiquitylation Phosphositeplus.1010730 21890473 Q93008 1593 S Phosphorylation Phosphositeplus.1010730 22115753 Q93008 1600 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;19690332;20363803;21406692;21659604;22115753 Q93008 1609 S Phosphorylation Phosphositeplus.1010730 18669648;19690332;21406692 Q93008 2443 S Phosphorylation Phosphositeplus.1010730 18669648;19276368;21406692;21659604 Q93008 2563 S Phosphorylation Phosphositeplus.1010730 16964243;18669648;18691976;19664994;20068231;21406692 Q93008 2438 T Phosphorylation Phosphositeplus.1010730 21659604 Q93008 2567 T Phosphorylation Phosphositeplus.1010730 20068231 Q93008 590 T Phosphorylation Phosphositeplus.1010730 18691976 Q93008 2439 Y Phosphorylation Phosphositeplus.1010730 19276368 Q93008 2556 Y Phosphorylation Phosphositeplus.1010730 15592455;18083107;18180459;20007894 Q93008 1767 N N-linked Glycosylation HPRD 9.0 19349973 Q93008 313 S Phosphorylation HPRD 9.0 20068231 Q93008 702 S Phosphorylation HPRD 9.0 20068231 Q93008 1593 S Phosphorylation HPRD 9.0 17322306,18669648,18691976,19651622,18452278,18767875 Q93008 1593 S Phosphorylation HPRD 9.0 19651622,18452278 Q93008 1600 S Phosphorylation HPRD 9.0 17322306,18669648,18691976,19651622,18452278,18767875 Q93008 1609 S Phosphorylation HPRD 9.0 18669648 Q93008 2443 S Phosphorylation HPRD 9.0 17081983,18669648,19651622,17693683 Q93008 2448 S Phosphorylation HPRD 9.0 18691976 Q93008 2563 S Phosphorylation HPRD 9.0 18669648,19413330,18691976,19664994,16964243,18452278,20068231 Q93008 590 T Phosphorylation HPRD 9.0 18691976 Q93008 2567 T Phosphorylation HPRD 9.0 20068231 Q93008 701 Y Phosphorylation HPRD 9.0 20068231 Q93008 2402 Y Phosphorylation HPRD 9.0 20068231 Q93008 2556 Y Phosphorylation HPRD 9.0 15592455 Q93008 1593 S Phosphorylation SysPTM 1.1 17322306 Q93008 1600 S Phosphorylation SysPTM 1.1 18691976 Q93008 2443 S Phosphorylation SysPTM 1.1 17693683 Q93008 2563 S Phosphorylation SysPTM 1.1 18691976 Q93008 590 T Phosphorylation SysPTM 1.1 18691976

PTM - HPRD Symbol Position Code Type References USP9X 1586 S Phosphorylation 19651622,18452278 USP9X 1593 S Phosphorylation 17322306,18669648,18691976,19651622,18452278,18767875 USP9X 1593 S Phosphorylation 19651622,18452278 USP9X 1600 S Phosphorylation 17322306,18669648,18691976,19651622,18452278,18767875 USP9X 1602 S Phosphorylation 18669648 USP9X 1609 S Phosphorylation 18669648 USP9X 1760 N Glycosylation 19349973 USP9X 1767 N Glycosylation 19349973 USP9X 2395 Y Phosphorylation 20068231 USP9X 2402 Y Phosphorylation 20068231 USP9X 2436 S Phosphorylation 17081983,18669648,19651622,17693683 USP9X 2441 S Phosphorylation 18691976 USP9X 2443 S Phosphorylation 17081983,18669648,19651622,17693683 USP9X 2448 S Phosphorylation 18691976 USP9X 2533 Y Phosphorylation 15592455 USP9X 2540 S Phosphorylation 18669648,19413330,18691976,19664994,16964243,18452278,20068231 USP9X 2540 Y Phosphorylation 15592455 USP9X 2544 T Phosphorylation 20068231 USP9X 2547 S Phosphorylation 18669648,19413330,18691976,19664994,16964243,18452278,20068231 USP9X 2549 Y Phosphorylation 15592455 USP9X 2551 T Phosphorylation 20068231 USP9X 2556 S Phosphorylation 18669648,19413330,18691976,19664994,16964243,18452278,20068231 USP9X 2556 Y Phosphorylation 15592455 USP9X 2560 T Phosphorylation 20068231 USP9X 2563 S Phosphorylation 18669648,19413330,18691976,19664994,16964243,18452278,20068231 USP9X 2567 T Phosphorylation 20068231 USP9X 306 S Phosphorylation 20068231 USP9X 313 S Phosphorylation 20068231 USP9X 583 T Phosphorylation 18691976 USP9X 590 T Phosphorylation 18691976 USP9X 694 Y Phosphorylation 20068231 USP9X 695 S Phosphorylation 20068231 USP9X 701 Y Phosphorylation 20068231 USP9X 702 S Phosphorylation 20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q93008 2540 S 18669648 HTP Phosphorylation Q93008 2540 S 16964243 HTP Phosphorylation Q93008 1602 S 18669648 HTP Phosphorylation Q93008 2436 S 18669648 HTP Phosphorylation Q93008 1593 S 18669648 HTP Phosphorylation Q93008 1593 S 17081983 HTP Phosphorylation Q93008 2533 Y 15592455 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q93008 588 Phosphoserine 23186163 Q93008 590 Phosphothreonine 23186163 Q93008 1600 Phosphoserine 17081983;18669648;18691976;19690332;21406692;23186163 Q93008 2443 Phosphoserine 17693683;18669648;21406692;23186163 Q93008 2556 Phosphotyrosine 15592455 Q93008 2563 Phosphoserine 16964243;18669648;20068231;23186163 Q93008 2567 Phosphothreonine 20068231

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type USP9X IPI00003964 S 1600 DVDDDMpSGDEKQD phosphorylation USP9X IPI00003964 S 2547 EGSEEVpSPPQTKD phosphorylation USP9X IPI00003964 T 2551 EVSPPQpTKDQ___ phosphorylation USP9X IPI00003964 T 590 PQNLSQpTQRSPHV phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 8239 113867 - K Ubiquitination 22118674 8239 113867 - K Ubiquitination 18781797 8239 113867 - K Ubiquitination 21987572 8239 113867 - K Sumoylation 21693764 8239 113867 - K Ubiquitination 21139048 8239 113867 - K Ubiquitination 21890473 8239 113867 - K Ubiquitination 21906983 8239 113867 - K Ubiquitination 22053931 8239 113867 - K Ubiquitination 23000965 8239 113867 - K Ubiquitination 24816145 8239 113867 - K Neddylation 18247557

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID USP9X Homo sapiens (Human) 2301; 1370; 1632; 1811; 1627; 1712; 218; 1798; 2360; 571; 1862; 1722; 1045; 329 "2301,NSEETVKLLRFCC; 1370,TARERAKHSGDYF; 1632,DKPALSKTEDRKE; 1811,ERECAIKFNDYFE; 1627,PQQFEDKPALSKT; 1712,HPAMLSKVLGGSF; 218,PDPRSPKGWLVDL; 1798,VLAIQLKRFDYDW; 2360,RIHNALKGIPDDR; 571,WVIPALKQIREIC; 1862,ETAGSTKYRLVGV; 1722,GSFADQKICQGCP; 1045,GARVLMKLMPPDS; 329,GQEETVKNLEIFR" 2301:21890473; 1370:21890473; 1632:21890473; 1811:21890473; 1627:21890473; 1712:21890473; 218:21890473; 1798:21890473; 2360:21890473; 571:21890473; 1862:21890473; 1722:21890473; 1045:21890473; 329:21890473 USP9X Homo sapiens (Human) 424; 218; 315; 486; 571; 794; 329 "424,RFVIKEKALTLQD; 218,PDPRSPKGWLVDL; 315,MIIKSLKNLASRV; 486,SKKQREKLLELIR; 571,WVIPALKQIREIC; 794,RAIDLLKEIYTNL; 329,GQEETVKNLEIFR" 424:21890473; 218:21890473; 315:21890473; 486:21890473; 571:21890473; 794:21890473; 329:21890473 USP9X Homo sapiens (Human) 1620 "1620,PQQFEDKPALSKT" 1620:21139048 USP9X Homo sapiens (Human) 2301; 1370; 1632; 1811; 1627; 218; 1712; 1935; 1798; 2360; 1862; 1722; 571; 1045; 329 "2301,NSEETVKLLRFCC; 1370,TARERAKHSGDYF; 1632,DKPALSKTEDRKE; 1811,ERECAIKFNDYFE; 1627,PQQFEDKPALSKT; 218,PDPRSPKGWLVDL; 1712,HPAMLSKVLGGSF; 1935,VFDHMMKRMSYRR; 1798,VLAIQLKRFDYDW; 2360,RIHNALKGIPDDR; 1862,ETAGSTKYRLVGV; 1722,GSFADQKICQGCP; 571,WVIPALKQIREIC; 1045,GARVLMKLMPPDS; 329,GQEETVKNLEIFR" "2301:21890473; 1370:21890473; 1632:21890473,21906983; 1811:21890473; 1627:21890473,21906983; 218:21890473; 1712:21890473; 1935:21906983; 1798:21890473; 2360:21890473; 1862:21890473; 1722:21890473; 571:21890473; 1045:21890473; 329:21890473" USP9X Homo sapiens (Human) 2301; 1370; 1632; 1811; 1627; 218; 1712; 1935; 1798; 2360; 1862; 1722; 571; 1045; 329 "2301,NSEETVKLLRFCC; 1370,TARERAKHSGDYF; 1632,DKPALSKTEDRKE; 1811,ERECAIKFNDYFE; 1627,PQQFEDKPALSKT; 218,PDPRSPKGWLVDL; 1712,HPAMLSKVLGGSF; 1935,VFDHMMKRMSYRR; 1798,VLAIQLKRFDYDW; 2360,RIHNALKGIPDDR; 1862,ETAGSTKYRLVGV; 1722,GSFADQKICQGCP; 571,WVIPALKQIREIC; 1045,GARVLMKLMPPDS; 329,GQEETVKNLEIFR" "2301:21890473; 1370:21890473; 1632:21890473,21906983; 1811:21890473; 1627:21890473,21906983; 218:21890473; 1712:21890473; 1935:21906983; 1798:21890473; 2360:21890473; 1862:21890473; 1722:21890473; 571:21890473; 1045:21890473; 329:21890473" USP9X Homo sapiens (Human) 2301; 1370; 1632; 1811; 1627; 1712; 218; 1798; 2360; 571; 1862; 1722; 1045; 329 "2301,NSEETVKLLRFCC; 1370,TARERAKHSGDYF; 1632,DKPALSKTEDRKE; 1811,ERECAIKFNDYFE; 1627,PQQFEDKPALSKT; 1712,HPAMLSKVLGGSF; 218,PDPRSPKGWLVDL; 1798,VLAIQLKRFDYDW; 2360,RIHNALKGIPDDR; 571,WVIPALKQIREIC; 1862,ETAGSTKYRLVGV; 1722,GSFADQKICQGCP; 1045,GARVLMKLMPPDS; 329,GQEETVKNLEIFR" 2301:21890473; 1370:21890473; 1632:21890473; 1811:21890473; 1627:21890473; 1712:21890473; 218:21890473; 1798:21890473; 2360:21890473; 571:21890473; 1862:21890473; 1722:21890473; 1045:21890473; 329:21890473 USP9X Homo sapiens (Human) 2301; 1370; 1632; 1811; 1627; 1712; 218; 1798; 2360; 571; 1862; 1722; 1045; 329 "2301,NSEETVKLLRFCC; 1370,TARERAKHSGDYF; 1632,DKPALSKTEDRKE; 1811,ERECAIKFNDYFE; 1627,PQQFEDKPALSKT; 1712,HPAMLSKVLGGSF; 218,PDPRSPKGWLVDL; 1798,VLAIQLKRFDYDW; 2360,RIHNALKGIPDDR; 571,WVIPALKQIREIC; 1862,ETAGSTKYRLVGV; 1722,GSFADQKICQGCP; 1045,GARVLMKLMPPDS; 329,GQEETVKNLEIFR" 2301:21890473; 1370:21890473; 1632:21890473; 1811:21890473; 1627:21890473; 1712:21890473; 218:21890473; 1798:21890473; 2360:21890473; 571:21890473; 1862:21890473; 1722:21890473; 1045:21890473; 329:21890473