PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus RSPO1 HECTD3 26186194 biogrid exp P P P P P P P P G P P P P P P P P P P P G BRCA1 HECTD3 22990118 biogrid exp P P G GP P P P P P P P GP P P P P P P P P P GP HECTD3 MAVS 21903422 innatedb,biogrid,intact exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G HSP90AB1 HECTD3 22939624 intact exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G CASP8 HECTD3 24287696 biogrid exp GP GP G GP P GP GP GP GP GP GP G GP P GP P G GP GP GP P GP G GP GP G HSP90AA1 HECTD3 22939624 biogrid exp GP GP G G G GP G G GP G G G G G G G GP G G G GP G G G G G G GP G SMAD2 HECTD3 20195357 intact exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G CCDC130 HECTD3 26186194 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G HECTD3 MALT1 23358872 biogrid exp G G GP G G G G G G GP G G G G G G G G G G G G GP G NCAPH HECTD3 22863883 biogrid exp G GP P P P G P P P GP CCNG1 HECTD3 26186194 biogrid exp GP GP G G GP GP G G GP G GP G GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G HECTD3 PARD6B 26186194 biogrid exp P P P P P P GP G GP P G GP P P GP GP GP GP P GP GP GP G RPS4X HECTD3 26186194 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP G GP GP G HECTD3 MRM1 26186194 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G NTRK1 HECTD3 25921289 biogrid exp P P P G P P P P P P P P P P P P P P P WNT9B HECTD3 26186194 biogrid exp P TP53 HECTD3 23358872 biogrid exp G G G G G G G G G G G G G G G GP G G G G GP G G G GP G G GP G HECTD3 ZNF317 26186194 biogrid exp G GP G G G GP G GP GP G GP G GP G G GP GP G P G GP G GP GP GP G GP GP G FOSL2 HECTD3 26186194 biogrid exp GP GP G G GP GP G G GP GP G GP GP G GP GP G G G GP GP GP G GP G GP G HECTD3 TRMT10C 26186194 biogrid exp GP GP G G GP GP G GP GP G GP G GP GP G GP GP G GP G GP G GP GP GP G GP GP G HECTD3 POGLUT1 26186194 biogrid exp G GP G GP G G P GP G GP G GP GP G GP G G GP G GP GP P P GP G GP P HECTD3 SCCPDH 26186194 biogrid exp G GP G G G G G G G G G GP GP GP G G G GP G G G G G G G G GP GP G HECTD3 ZDHHC17 24705354 biogrid,intact exp G GP G G GP GP G G GP G GP GP GP GP G GP GP G GP G GP GP GP G GP G GP GP G HECTD3 STX8 18821010, 21836163 biogrid exp, pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G ZZEF1 HECTD3 22863883, 23023127 biogrid exp, pred G GP G G G G G G GP G G G G G G GP G G G G GP G G G GP G GP GP G HECTD3 TRIOBP 18194665, 21836163 biogrid exp, pred GP GP G G GP GP G GP GP G GP GP GP G G GP GP GP GP G GP GP GP GP GP G GP GP G UBE2G1 HECTD3 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G CUL7 HECTD3 23023127 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G APBB1 HECTD3 23023127 pred GP GP G G GP GP G GP GP G GP GP GP G G G GP G GP G GP GP GP GP G G GP GP G UBE2C HECTD3 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G UBE2M HECTD3 16082366 pred GP GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP G GP GP GP G GP GP G UBE2L3 HECTD3 16082366 pred GP G G G G GP G GP GP G GP G G G G GP GP G GP G G G GP GP GP G GP GP G HECTD3 UBE2T 16082366 pred G G G GP G GP P P P GP GP P GP P G GP P GP G UBE2E1 HECTD3 16082366 pred GP GP G G G GP G G GP G GP G G GP G GP GP GP G G GP G G GP GP G GP GP G HECTD3 AKTIP 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G EGF HECTD3 23023127 pred P P P P GP P GP P P P G P P GP P GP GP GP GP P P HERC2 HECTD3 23023127 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G HECTD3 UBE2F 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP G GP G GP GP GP GP GP G GP GP G HECTD3 UFC1 16082366 pred GP GP G G G GP G GP GP G GP G GP G G GP GP G GP G GP GP GP GP GP G GP GP G UBE2A HECTD3 16082366 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G UBE2V2 HECTD3 16082366 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G UBE2D1 HECTD3 16082366 pred GP G G G GP GP G G GP G GP GP GP GP G G GP GP GP G GP GP G GP G G GP GP G UBE2B HECTD3 16082366 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G UBE2N HECTD3 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP G GP G GP GP GP GP GP G GP GP G HECTD3 UBE2D4 16082366 pred GP G G G GP GP G G GP G GP GP GP GP G G GP GP GP G GP GP G GP G G GP GP G UBE2I HECTD3 16082366 pred GP GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP GP GP G GP G GP GP G HECTD3 UBE2Z 16082366 pred GP GP G G GP GP G G GP G GP GP GP GP G GP GP G GP G GP GP GP GP GP G GP GP G HECTD3 BIRC6 16082366 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G UBE2L6 HECTD3 16082366 pred GP GP G G GP GP G G GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP G GP GP G HECTD3 UBE2R2 16082366 pred G G G G GP G G G G G G G G GP G G G G G G G G G G G G GP G G FLNA HECTD3 23023127 pred G G G G GP GP G G GP G GP GP GP GP G G GP GP GP G GP G G G G G GP GP G HECTD3 CUL9 23023127 pred G GP G G G GP G GP GP G GP GP G G G G GP G G P GP GP G G G G GP G HECTD3 UBE2U 16082366 pred P P P P P P P P P P P P P P P P P P P GP HECTD3 UBE2E3 16082366 pred GP GP G G G GP G G GP G GP G G GP G GP GP GP G G GP G G GP GP G GP GP G UBE2S HECTD3 16082366 pred G G G G G G G G GP G G G GP G G G G G G G G G G G GP G HECTD3 TSG101 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G UBE2D2 HECTD3 16082366 pred GP G G G GP GP G G GP G GP GP GP GP G G GP GP GP G GP GP G GP G G GP GP G HECTD3 16082366 pred UBE2H HECTD3 16082366 pred GP GP G G GP GP G GP G G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G CDC34 HECTD3 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G HECTD3 ANAPC10 23023127 pred GP G G G GP GP G GP GP G GP GP GP GP G GP GP G GP G G GP GP GP GP G GP GP G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q5T447 P04637-7 two hybrid 23358872 direct interaction X 2 Q5T447 P07900-2 affinity chromatography technology 22939624 physical association X 2 Q5T447 Q9H2D6-5 two hybrid 18194665 direct interaction X 2 Q5T447 Q9H2D6-5 pull down 18194665 direct interaction X 2 Q5T447 Q9H2D6-5 affinity chromatography technology 18194665 physical association X 2 O43149 Q5T447 biochemical 22863883 colocalization X 2 Q8IUH5 Q5T447 two hybrid 24705354 direct interaction X 2 Q15003 Q5T447 biochemical 22863883 colocalization X 2 Q5T447 two hybrid 22990118 direct interaction X 2 Q5T447 Q14790-9 affinity chromatography technology 24287696 physical association X 2 Q5T447 Q14790-9 affinity chromatography technology 24287696 physical association X 2 Q5T447 Q14790-9 pull down 24287696 direct interaction X 2 Q7Z434-4 Q5T447 affinity chromatography technology 21903422 physical association X 2 P0CG48 Q5T447 affinity chromatography technology 21963094 physical association X 2 P0CG48 Q5T447 affinity chromatography technology 24816145 physical association X 2 P0CG48 Q5T447 affinity chromatography technology 22118674 physical association X 2 P0CG48 Q5T447 affinity chromatography technology 21139048 physical association X 2 Q9UNK0 Q5T447 affinity chromatography technology 18821010 physical association X 2 Q5T447 two hybrid 23358872 direct interaction X 2 Q5T447 affinity chromatography technology 23358872 physical association X 2 Q5T447 affinity chromatography technology 23358872 physical association X 2 Q5T447 A0A024RD80 luminescence based mammalian interactome mapping 22939624 physical association X 2 Q15796 Q5T447 display technology 20195357 physical association X 2 Q8IUH5 Q5T447 two hybrid array 24705354 physical association X 2 Q5T447 anti tag coimmunoprecipitation 21903422 association C 11

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B UBC HECTD3 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0415(enzymatic study) 21139048,21963094,22118674 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid)|(biogrid) BIOGRID_169905|BIOGRID_265766|BIOGRID_160547 bait|bait|bait prey|prey|prey p53 HECTD3 MI:0018(two hybrid) 23358872 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_249612 prey bait HECTD3 STX8 MI:0004(affinity chromatography technology) 18821010 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_116419 bait prey HSP90AB1 HECTD3 MI:0729(luminescence based mammalian interactome mapping) 22939624 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (intact) UniProt_125013 prey bait NCAPH HECTD3 MI:0401(biochemical) 22863883 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization) (biogrid) BIOGRID_300131 bait prey BRCA1 HECTD3 MI:0004(affinity chromatography technology) 22990118 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_245672 bait prey SMAD2 HECTD3 MI:0034(display technology) 20195357 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (intact) UniProt_105180 bait prey HECTD3 TRIOBP MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 18194665 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(biogrid)|(biogrid) BIOGRID_117597|BIOGRID_117596|BIOGRID_117598 prey|prey|prey bait|bait|bait ZZEF1 HECTD3 MI:0401(biochemical) 22863883 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization) (biogrid) BIOGRID_300132 bait prey HECTD3 ZDHHC17 MI:0397(two hybrid array)|MI:0397(two hybrid array) 24705354 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(intact) UniProt_133247|UniProt_176478 prey|prey bait|bait HECTD3 MALT1 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 23358872 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction) (biogrid)|(biogrid)|(biogrid) BIOGRID_249615|BIOGRID_249614|BIOGRID_249613 prey|bait|bait bait|prey|prey CASP8 HECTD3 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 24287696 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid)|(biogrid) BIOGRID_310344|BIOGRID_310342|BIOGRID_310343 bait|prey|bait prey|bait|prey HECTD3 MAVS MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation) 21903422 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0914(association)|MI:0914(association) (biogrid)|(intact)|(intact) BIOGRID_155626|UniProt_115769|UniProt_172511 prey|prey|prey bait|bait|bait HSP90AA1 HECTD3 MI:0004(affinity chromatography technology) 22939624 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_255430 bait prey

PPI - HINT Gene A Gene B PMID Method Quality WNT9B HECTD3 26186194 0004 HT ZZEF1 HECTD3 22863883 0401 HT CCDC130 HECTD3 26186194 0004 HT FOSL2 HECTD3 26186194 0004 HT CCNG1 HECTD3 26186194 0004 HT RPS4X HECTD3 26186194 0004 HT NCAPH HECTD3 22863883 0401 HT RSPO1 HECTD3 26186194 0004 HT HECTD3 MRM1 26186194 0004 HT HECTD3 TRMT10C 26186194 0004 HT HECTD3 POGLUT1 26186194 0004 HT HECTD3 SCCPDH 26186194 0004 HT HECTD3 ZNF317 26186194 0004 HT HECTD3 PARD6B 26186194 0004 HT WNT9B HECTD3 26186194 0004 HT ZZEF1 HECTD3 22863883 0401 HT CCDC130 HECTD3 26186194 0004 HT FOSL2 HECTD3 26186194 0004 HT CCNG1 HECTD3 26186194 0004 HT RPS4X HECTD3 26186194 0004 HT NCAPH HECTD3 22863883 0401 HT RSPO1 HECTD3 26186194 0004 HT HECTD3 MRM1 26186194 0004 HT HECTD3 TRMT10C 26186194 0004 HT HECTD3 POGLUT1 26186194 0004 HT HECTD3 SCCPDH 26186194 0004 HT HECTD3 ZNF317 26186194 0004 HT HECTD3 PARD6B 26186194 0004 HT ZZEF1 HECTD3 22863883 0401 LC HSP90AB1 HECTD3 22939624 0729 HT BRCA1 HECTD3 22990118 0018 LC NCAPH HECTD3 22863883 0401 LC SMAD2 HECTD3 20195357 0034 LC Q53GA5 HECTD3 23358872 0018 LC HECTD3 ZDHHC17 24705354,24705354 0397,0018 HT HECTD3 TRIOBP 18194665,18194665,18194665 0096,0018,0004 LC HECTD3 MALT1 23358872,23358872 0004,0018 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q5T447 O14905 HECTD3 WNT9B 0.126 26186194 Q5T447 P04637 HECTD3 TP53 0.309 23358872 Q5T447 Q14790 HECTD3 CASP8 0.569 24287696 Q9UNK0 Q5T447 STX8 HECTD3 0.21 18821010 Q8NBL1 Q5T447 POGLUT1 HECTD3 0.126 26186194 Q8NBX0 Q5T447 SCCPDH HECTD3 0.126 26186194 Q5T447 P07900 HECTD3 HSP90AA1 0.126 22939624 Q5T447 O43149 HECTD3 ZZEF1 0.081 22863883 Q5T447 P08238 HECTD3 HSP90AB1 0.126 22939624 Q7L0Y3 Q5T447 TRMT10C HECTD3 0.126 26186194 Q6IN84 Q5T447 MRM1 HECTD3 0.126 26186194 Q5T447 P13994 HECTD3 CCDC130 0.126 26186194 Q5T447 P04629 HECTD3 NTRK1 0.126 25921289 Q7Z434 Q5T447 MAVS HECTD3 0.236 21903422 Q5T447 O60437 HECTD3 PPL 0.236 unassigned1304 Q8IUH5 Q5T447 ZDHHC17 HECTD3 0.286 24705354 Q9H2D6 Q5T447 TRIOBP HECTD3 0.623 18194665 Q96LJ8 Q5T447 UBXN10 HECTD3 0.126 26389662 Q5T447 P38398 HECTD3 BRCA1 0.183 22990118 Q96PQ6 Q5T447 ZNF317 HECTD3 0.126 26186194 Q9BYG5 Q5T447 PARD6B HECTD3 0.126 26186194 Q9UDY8 Q5T447 MALT1 HECTD3 0.569 23358872 Q5T447 P46379 HECTD3 BAG6 0.21 24981174 Q5T447 P62701 HECTD3 RPS4X 0.126 26186194 Q5T447 Q15003 HECTD3 NCAPH 0.081 22863883 Q5T447 P51959 HECTD3 CCNG1 0.126 26186194 Q5T447 Q15796 HECTD3 SMAD2 0.126 20195357 Q5T447 Q2MKA7 HECTD3 RSPO1 0.126 26186194 Q5T447 P15408 HECTD3 FOSL2 0.126 26186194

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 WNT9B HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database NTRK1 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database TP53 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database HSP90AA1 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database HSP90AB1 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database UBC HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database CCDC130 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database FOSL2 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database BRCA1 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database CCNG1 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database RPS4X HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database CASP8 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database SMAD2 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database RSPO1 HECTD3 MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 MRM1 MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 TRMT10C MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 MAVS MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 ZDHHC17 MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 POGLUT1 MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 SCCPDH MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 UBXN10 MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 ZNF317 MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 PARD6B MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 TRIOBP MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 MALT1 MI:0045 experimental interaction detection MI:0461 interaction database HECTD3 STX8 MI:0045 experimental interaction detection MI:0461 interaction database