PTM - CPLM Gene Name UniProt ID Type Position Code UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Acetylation 236 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Acetylation 241 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Acetylation 271 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Acetylation 324 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Acetylation 617 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Sumoylation 540 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 65 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 77 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 241 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 245 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 253 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 257 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 271 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 316 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 324 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 336 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 420 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 467 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 472 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 153 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 164 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 505 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 260 K UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 Ubiquitination 66 K

PTM - dbPAF Gene Name UniProt ID Position Type Species UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 178 T Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 207 S Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 229 S Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 249 Y Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 265 Y Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 289 S Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 294 T Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 297 S Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 328 T Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 507 S Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 577 S Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 578 T Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 592 S Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 597 S Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 598 S Homo sapiens(Human) UBA2; SAE2; UBLE1B; HRIHFB2115 Q9UBT2 604 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9UBT2 K77-ac Acetylation RSKAQVAkESVLQFY Q9UBT2 K236-ac Acetylation sNEDGDIkRISTkEW Q9UBT2 K241-ac Acetylation DIkRISTkEWAkSTG Q9UBT2 K253-ac Acetylation STGyDPVkLFTkLFK Q9UBT2 K271-ac Acetylation RYLLTMDkLWRkRKP Q9UBT2 K324-ac Acetylation SYARLFSkSIEtLRV Q9UBT2 K336-ac Acetylation LRVHLAEkGDGAELI Q9UBT2 K617-ac Acetylation RkRkLDEkENLsAkR Q9UBT2 K623-ac Acetylation EkENLsAkRsRIEQk Q9UBT2 R210-m1 Methylation DQEVsPDrADPEAAW Q9UBT2 K271-m1 Methylation RYLLTMDkLWRkRKP Q9UBT2 T156-p Phosphorylation TTIkkGVtECyECHP Q9UBT2 Y159-p Phosphorylation kkGVtECyECHPkPT Q9UBT2 S207-p Phosphorylation EDADQEVsPDrADPE Q9UBT2 S229-p Phosphorylation AEARARAsNEDGDIk Q9UBT2 Y249-p Phosphorylation EWAkSTGyDPVkLFT Q9UBT2 S289-p Phosphorylation LDWAEVQsQGEEtNA Q9UBT2 T294-p Phosphorylation VQsQGEEtNASDQQN Q9UBT2 T328-p Phosphorylation LFSkSIEtLRVHLAE Q9UBT2 S492-p Phosphorylation GKGTILIssEEGETE Q9UBT2 S493-p Phosphorylation KGTILIssEEGETEA Q9UBT2 S507-p Phosphorylation ANNHKKLsEFGIRNG Q9UBT2 T578-p Phosphorylation DDGAQPStSTAQEQD Q9UBT2 S592-p Phosphorylation DDVLIVDsDEEDSsN Q9UBT2 S598-p Phosphorylation DsDEEDSsNNADVSE Q9UBT2 S621-p Phosphorylation LDEkENLsAkRsRIE Q9UBT2 S625-p Phosphorylation ENLsAkRsRIEQkEE Q9UBT2 K190-sm Sumoylation IHCIVWAkYLFNQLF Q9UBT2 K236-sm Sumoylation sNEDGDIkRISTkEW Q9UBT2 K257-sm Sumoylation DPVkLFTkLFKDDIR Q9UBT2 K271-sm Sumoylation RYLLTMDkLWRkRKP Q9UBT2 K275-sm Sumoylation TMDkLWRkRKPPVPL Q9UBT2 K316-sm Sumoylation DQQVLDVkSYARLFS Q9UBT2 K371-sm Sumoylation HIFSMNMkSRFDIKS Q9UBT2 K540-sm Sumoylation LHSEDLGkDVEFEVV Q9UBT2 K611-sm Sumoylation SEEERSRkRkLDEkE Q9UBT2 K613-sm Sumoylation EERSRkRkLDEkENL Q9UBT2 K617-sm Sumoylation RkRkLDEkENLsAkR Q9UBT2 K623-sm Sumoylation EkENLsAkRsRIEQk Q9UBT2 K630-sm Sumoylation kRsRIEQkEELDDVI Q9UBT2 K65-ub Ubiquitination NRQFLFQkKHVGRSK Q9UBT2 K77-ub Ubiquitination RSKAQVAkESVLQFY Q9UBT2 K86-ub Ubiquitination SVLQFYPkANIVAYH Q9UBT2 K152-ub Ubiquitination LGQVTTIkkGVtECy Q9UBT2 K153-ub Ubiquitination GQVTTIkkGVtECyE Q9UBT2 K164-ub Ubiquitination ECyECHPkPTQRTFP Q9UBT2 K241-ub Ubiquitination DIkRISTkEWAkSTG Q9UBT2 K245-ub Ubiquitination ISTkEWAkSTGyDPV Q9UBT2 K253-ub Ubiquitination STGyDPVkLFTkLFK Q9UBT2 K257-ub Ubiquitination DPVkLFTkLFKDDIR Q9UBT2 K271-ub Ubiquitination RYLLTMDkLWRkRKP Q9UBT2 K316-ub Ubiquitination DQQVLDVkSYARLFS Q9UBT2 K324-ub Ubiquitination SYARLFSkSIEtLRV Q9UBT2 K336-ub Ubiquitination LRVHLAEkGDGAELI Q9UBT2 K409-ub Ubiquitination GLKILSGkIDQCRTI Q9UBT2 K420-ub Ubiquitination CRTIFLNkQPNPRKK Q9UBT2 K467-ub Ubiquitination TVLTLQDkIVKEkFA Q9UBT2 K472-ub Ubiquitination QDkIVKEkFAMVAPD

PTM - dbPTM UniProt ID Position Code Type Resources References Q9UBT2 271 K Acetylation Swiss-Prot 1010711 19608861 Q9UBT2 324 K Acetylation Swiss-Prot 1010711 19608861 Q9UBT2 592 S Phosphorylation Swiss-Prot 1010711 18669648;19413330 Q9UBT2 617 K Acetylation Swiss-Prot 1010711 19608861 Q9UBT2 207 S Phosphorylation PhosphoELM.10011 15302935 Q9UBT2 592 S Phosphorylation PhosphoELM.10011 18669648 Q9UBT2 236 K Sumoylation Phosphositeplus.1010730 19721078 Q9UBT2 257 K Sumoylation Phosphositeplus.1010730 19721078 Q9UBT2 271 K Sumoylation Phosphositeplus.1010730 19721078 Q9UBT2 540 K Sumoylation Phosphositeplus.1010730 20797634 Q9UBT2 623 K Sumoylation Phosphositeplus.1010730 19721078 Q9UBT2 630 K Sumoylation Phosphositeplus.1010730 19721078 Q9UBT2 241 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 245 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 253 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 257 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 271 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 316 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UBT2 324 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 336 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 420 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 472 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 65 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 77 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UBT2 236 K Acetylation Phosphositeplus.1010730 20167786 Q9UBT2 241 K Acetylation Phosphositeplus.1010730 20167786 Q9UBT2 271 K Acetylation Phosphositeplus.1010730 19608861 Q9UBT2 324 K Acetylation Phosphositeplus.1010730 19608861 Q9UBT2 617 K Acetylation Phosphositeplus.1010730 19608861 Q9UBT2 207 S Phosphorylation Phosphositeplus.1010730 15302935;22115753 Q9UBT2 289 S Phosphorylation Phosphositeplus.1010730 18077418 Q9UBT2 507 S Phosphorylation Phosphositeplus.1010730 20068231 Q9UBT2 592 S Phosphorylation Phosphositeplus.1010730 18669648;21659604 Q9UBT2 598 S Phosphorylation Phosphositeplus.1010730 20058876 Q9UBT2 578 T Phosphorylation Phosphositeplus.1010730 21659604 Q9UBT2 265 Y Phosphorylation Phosphositeplus.1010730 18083107 Q9UBT2 271 K Acetylation HPRD 9.0 19608861 Q9UBT2 324 K Acetylation HPRD 9.0 19608861 Q9UBT2 617 K Acetylation HPRD 9.0 19608861 Q9UBT2 207 S Phosphorylation HPRD 9.0 15302935,18452278 Q9UBT2 289 S Phosphorylation HPRD 9.0 18077418 Q9UBT2 507 S Phosphorylation HPRD 9.0 20068231 Q9UBT2 577 S Phosphorylation HPRD 9.0 18452278 Q9UBT2 592 S Phosphorylation HPRD 9.0 18669648,19413330,18452278 Q9UBT2 597 S Phosphorylation HPRD 9.0 18452278 Q9UBT2 598 S Phosphorylation HPRD 9.0 18452278,20058876 Q9UBT2 592 S Phosphorylation SysPTM 1.1 18669648

PTM - HPRD Symbol Position Code Type References UBA2 207 S Phosphorylation 15302935,18452278 UBA2 271 K Acetylation 19608861 UBA2 289 S Phosphorylation 18077418 UBA2 324 K Acetylation 19608861 UBA2 507 S Phosphorylation 20068231 UBA2 577 S Phosphorylation 18452278 UBA2 592 S Phosphorylation 18669648,19413330,18452278 UBA2 597 S Phosphorylation 18452278 UBA2 598 S Phosphorylation 18452278,20058876 UBA2 617 K Acetylation 19608861

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9UBT2 207 S 15302935 HTP Phosphorylation Q9UBT2 592 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9UBT2 207 Phosphoserine 24275569 Q9UBT2 271 N6-acetyllysine; alternate 19608861 Q9UBT2 507 Phosphoserine 20068231;23186163 Q9UBT2 592 Phosphoserine 18669648

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 10054 115365 - K Sumoylation 21693764 10054 115365 - K Ubiquitination 21139048 10054 115365 - K Ubiquitination 21890473 10054 115365 - K Ubiquitination 21906983 10054 115365 - K Sumoylation 19471022 10054 115365 - K Sumoylation 21252943 10054 115365 - K Ubiquitination 23000965 10054 115365 - K Ubiquitination 24816145 10054 115365 - K Sumoylation 25027693 10054 115365 - K Ubiquitination 25147182 10054 115365 - K Sumoylation 25391492

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID UBA2 Homo sapiens (Human) 324; 253; 257; 65; 472; 336; 77; 245; 271; 316; 420; 467; 241 "324,YARLFSKSIETLR; 253,TGYDPVKLFTKLF; 257,PVKLFTKLFKDDI; 65,RQFLFQKKHVGRS; 472,DKIVKEKFAMVAP; 336,RVHLAEKGDGAEL; 77,SKAQVAKESVLQF; 245,STKEWAKSTGYDP; 271,YLLTMDKLWRKRK; 316,QQVLDVKSYARLF; 420,RTIFLNKQPNPRK; 467,VLTLQDKIVKEKF; 241,IKRISTKEWAKST" "324:21890473; 253:21890473; 257:21890473; 65:21890473; 472:21890473; 336:21890473; 77:21890473; 245:21890473; 271:21890473; 316:21890473,21906983; 420:21890473; 467:21890473; 241:21890473" UBA2 Homo sapiens (Human) 324; 253; 257; 65; 472; 336; 77; 245; 271; 316; 420; 467; 241 "324,YARLFSKSIETLR; 253,TGYDPVKLFTKLF; 257,PVKLFTKLFKDDI; 65,RQFLFQKKHVGRS; 472,DKIVKEKFAMVAP; 336,RVHLAEKGDGAEL; 77,SKAQVAKESVLQF; 245,STKEWAKSTGYDP; 271,YLLTMDKLWRKRK; 316,QQVLDVKSYARLF; 420,RTIFLNKQPNPRK; 467,VLTLQDKIVKEKF; 241,IKRISTKEWAKST" 324:21890473; 253:21890473; 257:21890473; 65:21890473; 472:21890473; 336:21890473; 77:21890473; 245:21890473; 271:21890473; 316:21890473; 420:21890473; 467:21890473; 241:21890473 UBA2 Homo sapiens (Human) 324; 253; 257; 65; 472; 336; 77; 245; 271; 316; 420; 467; 241 "324,YARLFSKSIETLR; 253,TGYDPVKLFTKLF; 257,PVKLFTKLFKDDI; 65,RQFLFQKKHVGRS; 472,DKIVKEKFAMVAP; 336,RVHLAEKGDGAEL; 77,SKAQVAKESVLQF; 245,STKEWAKSTGYDP; 271,YLLTMDKLWRKRK; 316,QQVLDVKSYARLF; 420,RTIFLNKQPNPRK; 467,VLTLQDKIVKEKF; 241,IKRISTKEWAKST" 324:21890473; 253:21890473; 257:21890473; 65:21890473; 472:21890473; 336:21890473; 77:21890473; 245:21890473; 271:21890473; 316:21890473; 420:21890473; 467:21890473; 241:21890473 UBA2 Homo sapiens (Human) 324; 253; 257; 65; 472; 336; 77; 245; 271; 316; 420; 467; 241 "324,YARLFSKSIETLR; 253,TGYDPVKLFTKLF; 257,PVKLFTKLFKDDI; 65,RQFLFQKKHVGRS; 472,DKIVKEKFAMVAP; 336,RVHLAEKGDGAEL; 77,SKAQVAKESVLQF; 245,STKEWAKSTGYDP; 271,YLLTMDKLWRKRK; 316,QQVLDVKSYARLF; 420,RTIFLNKQPNPRK; 467,VLTLQDKIVKEKF; 241,IKRISTKEWAKST" 324:21890473; 253:21890473; 257:21890473; 65:21890473; 472:21890473; 336:21890473; 77:21890473; 245:21890473; 271:21890473; 316:21890473; 420:21890473; 467:21890473; 241:21890473 UBA2 Homo sapiens (Human) 324; 253; 257; 65; 472; 336; 77; 245; 271; 316; 420; 467; 241 "324,YARLFSKSIETLR; 253,TGYDPVKLFTKLF; 257,PVKLFTKLFKDDI; 65,RQFLFQKKHVGRS; 472,DKIVKEKFAMVAP; 336,RVHLAEKGDGAEL; 77,SKAQVAKESVLQF; 245,STKEWAKSTGYDP; 271,YLLTMDKLWRKRK; 316,QQVLDVKSYARLF; 420,RTIFLNKQPNPRK; 467,VLTLQDKIVKEKF; 241,IKRISTKEWAKST" 324:21890473; 253:21890473; 257:21890473; 65:21890473; 472:21890473; 336:21890473; 77:21890473; 245:21890473; 271:21890473; 316:21890473; 420:21890473; 467:21890473; 241:21890473 UBA2 Homo sapiens (Human) 324; 253; 257; 65; 472; 336; 77; 245; 271; 316; 420; 467; 241 "324,YARLFSKSIETLR; 253,TGYDPVKLFTKLF; 257,PVKLFTKLFKDDI; 65,RQFLFQKKHVGRS; 472,DKIVKEKFAMVAP; 336,RVHLAEKGDGAEL; 77,SKAQVAKESVLQF; 245,STKEWAKSTGYDP; 271,YLLTMDKLWRKRK; 316,QQVLDVKSYARLF; 420,RTIFLNKQPNPRK; 467,VLTLQDKIVKEKF; 241,IKRISTKEWAKST" 324:21890473; 253:21890473; 257:21890473; 65:21890473; 472:21890473; 336:21890473; 77:21890473; 245:21890473; 271:21890473; 316:21890473; 420:21890473; 467:21890473; 241:21890473