PTM - CPLM Gene Name UniProt ID Type Position Code HERC2 O95714 Ubiquitination 95 K HERC2 O95714 Ubiquitination 105 K HERC2 O95714 Ubiquitination 990 K HERC2 O95714 Ubiquitination 2657 K HERC2 O95714 Ubiquitination 3690 K HERC2 O95714 Ubiquitination 3780 K HERC2 O95714 Ubiquitination 3839 K HERC2 O95714 Ubiquitination 1503 K HERC2 O95714 Ubiquitination 3146 K HERC2 O95714 Ubiquitination 3382 K HERC2 O95714 Ubiquitination 4396 K HERC2 O95714 Ubiquitination 2817 K HERC2 O95714 Ubiquitination 661 K HERC2 O95714 Ubiquitination 683 K HERC2 O95714 Ubiquitination 3716 K HERC2 O95714 Ubiquitination 17 K HERC2 O95714 Ubiquitination 2338 K HERC2 O95714 Ubiquitination 351 K HERC2 O95714 Ubiquitination 3247 K HERC2 O95714 Ubiquitination 4402 K HERC2 O95714 Ubiquitination 1677 K HERC2 O95714 Ubiquitination 3141 K HERC2 O95714 Ubiquitination 653 K HERC2 O95714 Ubiquitination 3469 K HERC2 O95714 Ubiquitination 2008 K HERC2 O95714 Ubiquitination 2304 K HERC2 O95714 Ubiquitination 2976 K HERC2 O95714 Ubiquitination 2979 K HERC2 O95714 Ubiquitination 20 K HERC2 O95714 Ubiquitination 2893 K HERC2 O95714 Ubiquitination 4774 K HERC2 O95714 Ubiquitination 547 K HERC2 O95714 Ubiquitination 3554 K HERC2 O95714 Ubiquitination 72 K

PTM - dbPAF Gene Name UniProt ID Position Type Species HERC2 O95714 1021 S Homo sapiens(Human) HERC2 O95714 1023 T Homo sapiens(Human) HERC2 O95714 1365 S Homo sapiens(Human) HERC2 O95714 1366 S Homo sapiens(Human) HERC2 O95714 1370 T Homo sapiens(Human) HERC2 O95714 1472 T Homo sapiens(Human) HERC2 O95714 1539 S Homo sapiens(Human) HERC2 O95714 1577 S Homo sapiens(Human) HERC2 O95714 1588 S Homo sapiens(Human) HERC2 O95714 1601 S Homo sapiens(Human) HERC2 O95714 1791 S Homo sapiens(Human) HERC2 O95714 1814 T Homo sapiens(Human) HERC2 O95714 1835 S Homo sapiens(Human) HERC2 O95714 1938 S Homo sapiens(Human) HERC2 O95714 1942 S Homo sapiens(Human) HERC2 O95714 1944 T Homo sapiens(Human) HERC2 O95714 1948 S Homo sapiens(Human) HERC2 O95714 2003 S Homo sapiens(Human) HERC2 O95714 2011 S Homo sapiens(Human) HERC2 O95714 2063 T Homo sapiens(Human) HERC2 O95714 2454 S Homo sapiens(Human) HERC2 O95714 263 S Homo sapiens(Human) HERC2 O95714 2662 T Homo sapiens(Human) HERC2 O95714 272 T Homo sapiens(Human) HERC2 O95714 2772 S Homo sapiens(Human) HERC2 O95714 2788 S Homo sapiens(Human) HERC2 O95714 2928 S Homo sapiens(Human) HERC2 O95714 2940 S Homo sapiens(Human) HERC2 O95714 2942 S Homo sapiens(Human) HERC2 O95714 2953 S Homo sapiens(Human) HERC2 O95714 2956 T Homo sapiens(Human) HERC2 O95714 2978 S Homo sapiens(Human) HERC2 O95714 3241 S Homo sapiens(Human) HERC2 O95714 3386 S Homo sapiens(Human) HERC2 O95714 3462 S Homo sapiens(Human) HERC2 O95714 3480 T Homo sapiens(Human) HERC2 O95714 3485 T Homo sapiens(Human) HERC2 O95714 3492 S Homo sapiens(Human) HERC2 O95714 3647 S Homo sapiens(Human) HERC2 O95714 3674 S Homo sapiens(Human) HERC2 O95714 3677 S Homo sapiens(Human) HERC2 O95714 3678 S Homo sapiens(Human) HERC2 O95714 368 S Homo sapiens(Human) HERC2 O95714 372 S Homo sapiens(Human) HERC2 O95714 3756 S Homo sapiens(Human) HERC2 O95714 376 Y Homo sapiens(Human) HERC2 O95714 3764 S Homo sapiens(Human) HERC2 O95714 3769 S Homo sapiens(Human) HERC2 O95714 405 T Homo sapiens(Human) HERC2 O95714 4195 T Homo sapiens(Human) HERC2 O95714 4687 S Homo sapiens(Human) HERC2 O95714 4690 T Homo sapiens(Human) HERC2 O95714 4710 S Homo sapiens(Human) HERC2 O95714 4810 S Homo sapiens(Human) HERC2 O95714 4811 S Homo sapiens(Human) HERC2 O95714 4814 S Homo sapiens(Human) HERC2 O95714 4827 T Homo sapiens(Human) HERC2 O95714 647 T Homo sapiens(Human) HERC2 O95714 748 T Homo sapiens(Human) HERC2 O95714 986 T Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA O95714 K4249-ac Acetylation QVQGLQGkKVIAIAT O95714 K967-m1 Methylation EAKKEAQkEkEIDEQ O95714 K969-m1 Methylation KKEAQkEkEIDEQEA O95714 S263-p Phosphorylation RATRFLRsVVTGDVH O95714 T272-p Phosphorylation VTGDVHGtPATkGPG O95714 S368-p Phosphorylation HLLSGPLsPNESFLR O95714 T405-p Phosphorylation AHLDRLAtPCMPPLC O95714 T647-p Phosphorylation GGSDGCKtPKLIEkL O95714 T748-p Phosphorylation HFDTLRVtKPEPAAL O95714 T986-p Phosphorylation STFHRSRtPLDkDLI O95714 S1021-p Phosphorylation QLLRNIAsQtVARLK O95714 T1023-p Phosphorylation LRNIAsQtVARLKDV O95714 S1366-p Phosphorylation KDEDHCSsPGGtPAS O95714 T1370-p Phosphorylation HCSsPGGtPASKSRL O95714 T1472-p Phosphorylation IEQVKHRtLPKSVVD O95714 S1539-p Phosphorylation SKFKLLSsLPRWRRI O95714 S1577-p Phosphorylation EKIGNEEsDLEEACI O95714 S1588-p Phosphorylation EACILPHsPINVDKR O95714 S1601-p Phosphorylation KRPIAIKsPKDKWQP O95714 S1633-p Phosphorylation VQGLYPQsPLLSTIA O95714 S1791-p Phosphorylation RFLLVMLsMLTLQHG O95714 T1814-p Phosphorylation NSGMLALtQTALRLI O95714 S1835-p Phosphorylation VEEDMNAsAQGASAT O95714 S1938-p Phosphorylation LPAAAQPsAEDsDtE O95714 S1942-p Phosphorylation AQPsAEDsDtEDDsE O95714 T1944-p Phosphorylation PsAEDsDtEDDsEAE O95714 S1948-p Phosphorylation DsDtEDDsEAEQTER O95714 S2003-p Phosphorylation LLRMLVEsGTTDkTS O95714 S2011-p Phosphorylation GTTDkTSsPNRLVYR O95714 S2454-p Phosphorylation RVKRRKQsPVPALPI O95714 T2662-p Phosphorylation KYkWGSVtHQSVGVV O95714 S2772-p Phosphorylation KQLKRCHsSQPGMLL O95714 S2788-p Phosphorylation SWSRMVKsLNVSSSV O95714 S2928-p Phosphorylation AAVPFLAsDNEEEED O95714 S2953-p Phosphorylation KKAAGLEsAAtIRTK O95714 T2956-p Phosphorylation AGLEsAAtIRTKVFV O95714 S2978-p Phosphorylation QLGGLKGsKIKVPSF O95714 S3241-p Phosphorylation YFRLGHGsDVHVRKP O95714 S3386-p Phosphorylation SLAkILLsLDGNLAK O95714 S3462-p Phosphorylation VDIEDRLsPNPWQEk O95714 T3480-p Phosphorylation VSSEDAVtPSAVtPS O95714 T3485-p Phosphorylation AVtPSAVtPSAPSAs O95714 S3492-p Phosphorylation tPSAPSAsARPFIPV O95714 S3647-p Phosphorylation VEFDRQCsTERRHDP O95714 S3674-p Phosphorylation VRSGREWsDWssELR O95714 S3677-p Phosphorylation GREWsDWssELRIPG O95714 S3678-p Phosphorylation REWsDWssELRIPGD O95714 S3764-p Phosphorylation LAACAQLsALAAsHR O95714 S3769-p Phosphorylation QLsALAAsHRMWALQ O95714 T4195-p Phosphorylation PMKIDSLtGLGVVKV O95714 S4458-p Phosphorylation MSSFGPDsLLLPHRV O95714 S4566-p Phosphorylation WKQLAGMsLtIADLS O95714 T4568-p Phosphorylation QLAGMsLtIADLSEV O95714 Y4585-p Phosphorylation DFIPGLMyIRDNEAT O95714 Y4796-p Phosphorylation KSIDTDDyARIALTG O95714 S4810-p Phosphorylation GEPAADDssDDsDNE O95714 S4811-p Phosphorylation EPAADDssDDsDNED O95714 S4814-p Phosphorylation ADDssDDsDNEDVDS O95714 T4827-p Phosphorylation DSFASDStQDYLTGH O95714 K72-ub Ubiquitination SVEPSGTkkEDLNDk O95714 K73-ub Ubiquitination VEPSGTkkEDLNDkE O95714 K79-ub Ubiquitination kkEDLNDkEKKDEEE O95714 K95-ub Ubiquitination PAPIYRAkSILDSWV O95714 K105-ub Ubiquitination LDSWVWGkQPDVNEL O95714 K276-ub Ubiquitination VHGtPATkGPGSIPL O95714 K547-ub Ubiquitination VISAFSGkQAGKHVV O95714 K653-ub Ubiquitination KtPKLIEkLQDLDVV O95714 K990-ub Ubiquitination RSRtPLDkDLINTGI O95714 K1503-ub Ubiquitination QEQGRSYkEVCAPVI O95714 K2008-ub Ubiquitination VEsGTTDkTSsPNRL O95714 K2657-ub Ubiquitination SVTTPKYkWGSVtHQ O95714 K3067-ub Ubiquitination TALTVDGkVFSWGEG O95714 K3078-ub Ubiquitination WGEGDDGkLGHFSRM O95714 K3141-ub Ubiquitination GDNTTQLkPKMVkVL O95714 K3146-ub Ubiquitination QLkPKMVkVLLGHRV O95714 K3382-ub Ubiquitination PNRPSLAkILLsLDG O95714 K3469-ub Ubiquitination sPNPWQEkREIVSSE O95714 K3690-ub Ubiquitination PGDELKWkFISDGSV O95714 K3780-ub Ubiquitination WALQRLRkLLTTEFG O95714 K3839-ub Ubiquitination DPIVRGGkQLLHSPF

PTM - dbPTM UniProt ID Position Code Type Resources References O95714 1577 S Phosphorylation Swiss-Prot 1010711 17081983 O95714 1588 S Phosphorylation Swiss-Prot 1010711 17081983 O95714 1942 S Phosphorylation Swiss-Prot 1010711 19413330 O95714 1944 T Phosphorylation Swiss-Prot 1010711 19413330 O95714 2454 S Phosphorylation Swiss-Prot 1010711 19690332 O95714 2928 S Phosphorylation Swiss-Prot 1010711 17081983;18669648;18691976 O95714 4810 S Phosphorylation Swiss-Prot 1010711 18669648 O95714 4811 S Phosphorylation Swiss-Prot 1010711 18669648 O95714 4814 S Phosphorylation Swiss-Prot 1010711 18669648 O95714 4827 T Phosphorylation Swiss-Prot 1010711 20023648 O95714 1577 S Phosphorylation PhosphoELM.10011 17081983 O95714 1588 S Phosphorylation PhosphoELM.10011 17081983 O95714 1938 S Phosphorylation PhosphoELM.10011 18669648 O95714 1942 S Phosphorylation PhosphoELM.10011 18669648 O95714 1948 S Phosphorylation PhosphoELM.10011 18669648 O95714 2454 S Phosphorylation PhosphoELM.10011 17081983 O95714 2928 S Phosphorylation PhosphoELM.10011 18669648;17081983 O95714 2942 S Phosphorylation PhosphoELM.10011 18669648 O95714 4810 S Phosphorylation PhosphoELM.10011 18669648 O95714 4811 S Phosphorylation PhosphoELM.10011 18669648 O95714 4814 S Phosphorylation PhosphoELM.10011 18669648 O95714 1944 T Phosphorylation PhosphoELM.10011 18669648 O95714 105 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 O95714 2657 K Ubiquitylation Phosphositeplus.1010730 21890473 O95714 3690 K Ubiquitylation Phosphositeplus.1010730 21890473 O95714 3780 K Ubiquitylation Phosphositeplus.1010730 21890473 O95714 3839 K Ubiquitylation Phosphositeplus.1010730 21890473 O95714 72 K Ubiquitylation Phosphositeplus.1010730 21906983 O95714 95 K Ubiquitylation Phosphositeplus.1010730 21890473 O95714 990 K Ubiquitylation Phosphositeplus.1010730 21890473 O95714 1577 S Phosphorylation Phosphositeplus.1010730 17081983 O95714 1588 S Phosphorylation Phosphositeplus.1010730 17081983 O95714 1938 S Phosphorylation Phosphositeplus.1010730 19690332;20363803;22115753 O95714 1942 S Phosphorylation Phosphositeplus.1010730 20363803;21406692;21659604;22115753 O95714 1948 S Phosphorylation Phosphositeplus.1010730 18669648 O95714 2454 S Phosphorylation Phosphositeplus.1010730 19690332;20068231;22115753 O95714 2928 S Phosphorylation Phosphositeplus.1010730 17081983;18491316;18669648;18691976;20068231;20363803;21406692;21659604;22115753 O95714 3241 S Phosphorylation Phosphositeplus.1010730 21406692 O95714 4810 S Phosphorylation Phosphositeplus.1010730 18669648 O95714 4811 S Phosphorylation Phosphositeplus.1010730 18669648 O95714 4814 S Phosphorylation Phosphositeplus.1010730 18669648 O95714 1944 T Phosphorylation Phosphositeplus.1010730 20363803;21406692;22115753 O95714 272 T Phosphorylation Phosphositeplus.1010730 22067460 O95714 4827 T Phosphorylation Phosphositeplus.1010730 20023648;22508508 O95714 647 T Phosphorylation Phosphositeplus.1010730 21406692 O95714 376 Y Phosphorylation Phosphositeplus.1010730 18083107 O95714 1938 S Phosphorylation HPRD 9.0 18669648,19651622 O95714 1942 S Phosphorylation HPRD 9.0 17081983,18669648,19413330,19651622 O95714 1948 S Phosphorylation HPRD 9.0 17081983,18669648 O95714 2454 S Phosphorylation HPRD 9.0 19651622,20068231 O95714 2928 S Phosphorylation HPRD 9.0 17322306,18669648,18691976,18491316,19651622,20068231 O95714 2942 S Phosphorylation HPRD 9.0 18669648 O95714 4687 S Phosphorylation HPRD 9.0 20068231 O95714 4710 S Phosphorylation HPRD 9.0 20068231 O95714 4810 S Phosphorylation HPRD 9.0 18669648 O95714 4811 S Phosphorylation HPRD 9.0 18669648 O95714 4814 S Phosphorylation HPRD 9.0 18669648 O95714 1944 T Phosphorylation HPRD 9.0 18669648,19413330 O95714 4690 T Phosphorylation HPRD 9.0 20068231 O95714 1942 S Phosphorylation SysPTM 1.1 17081983 O95714 1948 S Phosphorylation SysPTM 1.1 17081983 O95714 2928 S Phosphorylation SysPTM 1.1 17081983;18691976;18669648;18491316 O95714 4810 S Phosphorylation SysPTM 1.1 18669648 O95714 4811 S Phosphorylation SysPTM 1.1 18669648 O95714 4814 S Phosphorylation SysPTM 1.1 18669648

PTM - HPRD Symbol Position Code Type References HERC2 1938 S Phosphorylation 18669648,19651622 HERC2 1942 S Phosphorylation 17081983,18669648,19413330,19651622 HERC2 1944 T Phosphorylation 18669648,19413330 HERC2 1948 S Phosphorylation 17081983,18669648 HERC2 2454 S Phosphorylation 19651622,20068231 HERC2 2928 S Phosphorylation 17322306,18669648,18691976,18491316,19651622,20068231 HERC2 2942 S Phosphorylation 18669648 HERC2 4687 S Phosphorylation 20068231 HERC2 4690 T Phosphorylation 20068231 HERC2 4710 S Phosphorylation 20068231 HERC2 4810 S Phosphorylation 18669648 HERC2 4811 S Phosphorylation 18669648 HERC2 4814 S Phosphorylation 18669648

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type O95714 1938 S 18669648 HTP Phosphorylation O95714 2928 S 18669648 HTP Phosphorylation O95714 2928 S 17081983 HTP Phosphorylation O95714 4811 S 18669648 HTP Phosphorylation O95714 1577 S 17081983 HTP Phosphorylation O95714 4810 S 18669648 HTP Phosphorylation O95714 4814 S 18669648 HTP Phosphorylation O95714 1942 S 18669648 HTP Phosphorylation O95714 1944 T 18669648 HTP Phosphorylation O95714 2454 S 17081983 HTP Phosphorylation O95714 1588 S 17081983 HTP Phosphorylation O95714 2942 S 18669648 HTP Phosphorylation O95714 1948 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs O95714 272 Phosphothreonine 23186163 O95714 647 Phosphothreonine 21406692 O95714 1944 Phosphothreonine 21406692 O95714 2454 Phosphoserine 17081983;19690332;20068231;23186163 O95714 2928 Phosphoserine 17081983;18669648;20068231;21406692;23186163 O95714 4810 Phosphoserine 18669648 O95714 4811 Phosphoserine 18669648 O95714 4814 Phosphoserine 18669648 O95714 4827 Phosphothreonine 20023648

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type HERC2 IPI00005826 S 1577 KIGNEEpSDLEEAC phosphorylation HERC2 IPI00005826 S 1588 ACILPHpSPINVDK phosphorylation HERC2 IPI00005826 S 2454 VKRRKQpSPVPALP phosphorylation HERC2 IPI00005826 S 2928 AVPFLApSDNEEEE phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 8924 114438 - K Ubiquitination 21139048 8924 114438 - K Ubiquitination 21890473 8924 114438 - K Ubiquitination 21906983 8924 114438 - K Sumoylation 22508508 8924 114438 - K Sumoylation 21110914 8924 114438 - K Ubiquitination 23000965

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID HERC2 Homo sapiens (Human) 3690; 2657; 990; 3780; 95; 3839; 105 "3690,GDELKWKFISDGS; 2657,VTTPKYKWGSVTH; 990,SRTPLDKDLINTG; 3780,ALQRLRKLLTTEF; 95,APIYRAKSILDSW; 3839,PIVRGGKQLLHSP; 105,DSWVWGKQPDVNE" 3690:21890473; 2657:21890473; 990:21890473; 3780:21890473; 95:21890473; 3839:21890473; 105:21890473 HERC2 Homo sapiens (Human) 3690; 2657; 990; 3780; 95; 72; 3839; 105 "3690,GDELKWKFISDGS; 2657,VTTPKYKWGSVTH; 990,SRTPLDKDLINTG; 3780,ALQRLRKLLTTEF; 95,APIYRAKSILDSW; 72,VEPSGTKKEDLND; 3839,PIVRGGKQLLHSP; 105,DSWVWGKQPDVNE" "3690:21890473; 2657:21890473; 990:21890473; 3780:21890473; 95:21890473; 72:21906983; 3839:21890473; 105:21890473,21906983"