PTM - CPLM Gene Name UniProt ID Type Position Code NEDD1 Q8NHV4 Ubiquitination 243 K NEDD1 Q8NHV4 Ubiquitination 391 K NEDD1 Q8NHV4 Ubiquitination 570 K

PTM - dbPAF Gene Name UniProt ID Position Type Species NEDD1 Q8NHV4 108 S Homo sapiens(Human) NEDD1 Q8NHV4 176 S Homo sapiens(Human) NEDD1 Q8NHV4 180 S Homo sapiens(Human) NEDD1 Q8NHV4 182 S Homo sapiens(Human) NEDD1 Q8NHV4 215 S Homo sapiens(Human) NEDD1 Q8NHV4 237 S Homo sapiens(Human) NEDD1 Q8NHV4 238 S Homo sapiens(Human) NEDD1 Q8NHV4 282 S Homo sapiens(Human) NEDD1 Q8NHV4 286 T Homo sapiens(Human) NEDD1 Q8NHV4 325 S Homo sapiens(Human) NEDD1 Q8NHV4 332 S Homo sapiens(Human) NEDD1 Q8NHV4 338 S Homo sapiens(Human) NEDD1 Q8NHV4 377 S Homo sapiens(Human) NEDD1 Q8NHV4 382 T Homo sapiens(Human) NEDD1 Q8NHV4 387 T Homo sapiens(Human) NEDD1 Q8NHV4 389 S Homo sapiens(Human) NEDD1 Q8NHV4 396 S Homo sapiens(Human) NEDD1 Q8NHV4 397 S Homo sapiens(Human) NEDD1 Q8NHV4 401 T Homo sapiens(Human) NEDD1 Q8NHV4 404 S Homo sapiens(Human) NEDD1 Q8NHV4 405 S Homo sapiens(Human) NEDD1 Q8NHV4 411 S Homo sapiens(Human) NEDD1 Q8NHV4 423 S Homo sapiens(Human) NEDD1 Q8NHV4 426 S Homo sapiens(Human) NEDD1 Q8NHV4 452 S Homo sapiens(Human) NEDD1 Q8NHV4 453 S Homo sapiens(Human) NEDD1 Q8NHV4 455 S Homo sapiens(Human) NEDD1 Q8NHV4 459 S Homo sapiens(Human) NEDD1 Q8NHV4 460 S Homo sapiens(Human) NEDD1 Q8NHV4 468 S Homo sapiens(Human) NEDD1 Q8NHV4 487 Y Homo sapiens(Human) NEDD1 Q8NHV4 493 S Homo sapiens(Human) NEDD1 Q8NHV4 496 S Homo sapiens(Human) NEDD1 Q8NHV4 505 T Homo sapiens(Human) NEDD1 Q8NHV4 510 S Homo sapiens(Human) NEDD1 Q8NHV4 515 T Homo sapiens(Human) NEDD1 Q8NHV4 516 S Homo sapiens(Human) NEDD1 Q8NHV4 518 S Homo sapiens(Human) NEDD1 Q8NHV4 519 S Homo sapiens(Human) NEDD1 Q8NHV4 522 T Homo sapiens(Human) NEDD1 Q8NHV4 525 S Homo sapiens(Human) NEDD1 Q8NHV4 548 S Homo sapiens(Human) NEDD1 Q8NHV4 549 S Homo sapiens(Human) NEDD1 Q8NHV4 550 T Homo sapiens(Human) NEDD1 Q8NHV4 557 S Homo sapiens(Human) NEDD1 Q8NHV4 558 S Homo sapiens(Human) NEDD1 Q8NHV4 560 T Homo sapiens(Human) NEDD1 Q8NHV4 565 S Homo sapiens(Human) NEDD1 Q8NHV4 566 S Homo sapiens(Human) NEDD1 Q8NHV4 568 S Homo sapiens(Human) NEDD1 Q8NHV4 574 S Homo sapiens(Human) NEDD1 Q8NHV4 586 S Homo sapiens(Human) NEDD1 Q8NHV4 636 Y Homo sapiens(Human) NEDD1 Q8NHV4 637 S Homo sapiens(Human) NEDD1 Q8NHV4 81 T Homo sapiens(Human) NEDD1 Q8NHV4 88 T Homo sapiens(Human) NEDD1 Q8NHV4 89 S Homo sapiens(Human) NEDD1 Q8NHV4 91 Y Homo sapiens(Human) NEDD1 Q8NHV4 94 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q8NHV4 K494-ac Acetylation yMGKQEskDsFKQLA Q8NHV4 T81-p Phosphorylation LAEGQKQtCVNLNSt Q8NHV4 T88-p Phosphorylation tCVNLNStsMYLVsG Q8NHV4 S89-p Phosphorylation CVNLNStsMYLVsGG Q8NHV4 S94-p Phosphorylation StsMYLVsGGLNNTV Q8NHV4 S108-p Phosphorylation VNIWDLKsKRVHRSL Q8NHV4 S176-p Phosphorylation KYSLFKKsLLGsVsD Q8NHV4 S180-p Phosphorylation FKKsLLGsVsDNGIV Q8NHV4 S182-p Phosphorylation KsLLGsVsDNGIVTL Q8NHV4 S215-p Phosphorylation PASGICFsPVNELLF Q8NHV4 S282-p Phosphorylation YDLRMLKsPVKtISA Q8NHV4 T286-p Phosphorylation MLKsPVKtISAHKTS Q8NHV4 S325-p Phosphorylation PTTVNKRsVNVNAAs Q8NHV4 S332-p Phosphorylation sVNVNAAsGGVQNsG Q8NHV4 S338-p Phosphorylation AsGGVQNsGIVREAP Q8NHV4 S377-p Phosphorylation EKAGLPRsINTDtLS Q8NHV4 T382-p Phosphorylation PRsINTDtLSKEtDs Q8NHV4 T387-p Phosphorylation TDtLSKEtDsGkNQD Q8NHV4 S389-p Phosphorylation tLSKEtDsGkNQDFs Q8NHV4 S396-p Phosphorylation sGkNQDFssFDDtGK Q8NHV4 S397-p Phosphorylation GkNQDFssFDDtGKs Q8NHV4 T401-p Phosphorylation DFssFDDtGKssLGD Q8NHV4 S404-p Phosphorylation sFDDtGKssLGDMFs Q8NHV4 S405-p Phosphorylation FDDtGKssLGDMFsP Q8NHV4 S411-p Phosphorylation ssLGDMFsPIRDDAV Q8NHV4 S423-p Phosphorylation DAVVNKGsDEsIGKG Q8NHV4 S426-p Phosphorylation VNKGsDEsIGKGDGF Q8NHV4 S460-p Phosphorylation STSVLHSsPLNVFMG Q8NHV4 S468-p Phosphorylation PLNVFMGsPGKEENE Q8NHV4 Y487-p Phosphorylation TAESKKIyMGKQEsk Q8NHV4 S493-p Phosphorylation IyMGKQEskDsFKQL Q8NHV4 S496-p Phosphorylation GKQEskDsFKQLAKL Q8NHV4 S510-p Phosphorylation LVTSGAEsGNLNtsP Q8NHV4 T515-p Phosphorylation AEsGNLNtsPsSNQt Q8NHV4 S516-p Phosphorylation EsGNLNtsPsSNQtR Q8NHV4 S518-p Phosphorylation GNLNtsPsSNQtRNs Q8NHV4 T522-p Phosphorylation tsPsSNQtRNsEKFE Q8NHV4 S525-p Phosphorylation sSNQtRNsEKFEKPE Q8NHV4 S548-p Phosphorylation CEPPINGsstPNPKI Q8NHV4 S549-p Phosphorylation EPPINGsstPNPKIA Q8NHV4 T550-p Phosphorylation PPINGsstPNPKIAs Q8NHV4 S557-p Phosphorylation tPNPKIAssVtAGVA Q8NHV4 S558-p Phosphorylation PNPKIAssVtAGVAs Q8NHV4 T560-p Phosphorylation PKIAssVtAGVAssL Q8NHV4 S565-p Phosphorylation sVtAGVAssLsEkIA Q8NHV4 S566-p Phosphorylation VtAGVAssLsEkIAD Q8NHV4 S568-p Phosphorylation AGVAssLsEkIADsI Q8NHV4 S574-p Phosphorylation LsEkIADsIGNNRQN Q8NHV4 S586-p Phosphorylation RQNAPLTsIQIRFIQ Q8NHV4 Y636-p Phosphorylation MHSLLERysVNEGLV Q8NHV4 S637-p Phosphorylation HSLLERysVNEGLVA Q8NHV4 K243-ub Ubiquitination TSSKKLVkTLVADTP Q8NHV4 K391-ub Ubiquitination SKEtDsGkNQDFssF Q8NHV4 K570-ub Ubiquitination VAssLsEkIADsIGN

PTM - dbPTM UniProt ID Position Code Type Resources References Q8NHV4 382 T Phosphorylation Swiss-Prot 1010711 19509060 Q8NHV4 397 S Phosphorylation Swiss-Prot 1010711 18691976;19509060 Q8NHV4 411 S Phosphorylation Swiss-Prot 1010711 18691976 Q8NHV4 426 S Phosphorylation Swiss-Prot 1010711 19509060 Q8NHV4 516 S Phosphorylation Swiss-Prot 1010711 18669648;18691976;19007248;19413330;19690332 Q8NHV4 550 T Phosphorylation Swiss-Prot 1010711 19509060 Q8NHV4 574 S Phosphorylation Swiss-Prot 1010711 19007248 Q8NHV4 637 S Phosphorylation Swiss-Prot 1010711 19509060 Q8NHV4 411 S Phosphorylation PhosphoELM.10011 18669648 Q8NHV4 423 S Phosphorylation PhosphoELM.10011 18669648 Q8NHV4 516 S Phosphorylation PhosphoELM.10011 18669648 Q8NHV4 243 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8NHV4 391 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8NHV4 570 K Ubiquitylation Phosphositeplus.1010730 21906983 Q8NHV4 108 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 215 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 282 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 325 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 332 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 338 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 377 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 389 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 396 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 397 S Phosphorylation Phosphositeplus.1010730 18691976;19509060;20363803;22115753;22595525 Q8NHV4 404 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 405 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 411 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;21690413;22595525 Q8NHV4 423 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 426 S Phosphorylation Phosphositeplus.1010730 19509060;22595525 Q8NHV4 460 S Phosphorylation Phosphositeplus.1010730 21690413;22595525 Q8NHV4 468 S Phosphorylation Phosphositeplus.1010730 22067460;22595525 Q8NHV4 493 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 496 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 510 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 516 S Phosphorylation Phosphositeplus.1010730 18669648;18691976;19415658;19664994;19664995;19690332;19691289;20068231;22067460;22115753;22595525 Q8NHV4 518 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 557 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 558 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 565 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 566 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 568 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 574 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 586 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 637 S Phosphorylation Phosphositeplus.1010730 19509060;22595525 Q8NHV4 89 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 94 S Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 286 T Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 382 T Phosphorylation Phosphositeplus.1010730 19509060;22595525 Q8NHV4 387 T Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 401 T Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 515 T Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 522 T Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 550 T Phosphorylation Phosphositeplus.1010730 19509060;21690413;22595525 Q8NHV4 560 T Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 81 T Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 88 T Phosphorylation Phosphositeplus.1010730 22595525 Q8NHV4 94 S Phosphorylation HPRD 9.0 20068231 Q8NHV4 237 S Phosphorylation HPRD 9.0 20068231 Q8NHV4 238 S Phosphorylation HPRD 9.0 20068231 Q8NHV4 397 S Phosphorylation HPRD 9.0 18691976 Q8NHV4 411 S Phosphorylation HPRD 9.0 18669648,18691976 Q8NHV4 423 S Phosphorylation HPRD 9.0 18669648 Q8NHV4 468 S Phosphorylation HPRD 9.0 19651622 Q8NHV4 516 S Phosphorylation HPRD 9.0 18669648,19413330,19415658,19007248,19664995,19691289,19664994,18691976,18452278,20068231 Q8NHV4 518 S Phosphorylation HPRD 9.0 18691976 Q8NHV4 574 S Phosphorylation HPRD 9.0 19007248 Q8NHV4 515 T Phosphorylation HPRD 9.0 18691976 Q8NHV4 91 Y Phosphorylation HPRD 9.0 20068231

PTM - HPRD Symbol Position Code Type References NEDD1 237 S Phosphorylation 20068231 NEDD1 238 S Phosphorylation 20068231 NEDD1 397 S Phosphorylation 18691976 NEDD1 411 S Phosphorylation 18669648,18691976 NEDD1 423 S Phosphorylation 18669648 NEDD1 468 S Phosphorylation 19651622 NEDD1 515 T Phosphorylation 18691976 NEDD1 516 S Phosphorylation 18669648,19413330,19415658,19007248,19664995,19691289,19664994,18691976,18452278,20068231 NEDD1 518 S Phosphorylation 18691976 NEDD1 574 S Phosphorylation 19007248 NEDD1 91 Y Phosphorylation 20068231 NEDD1 94 S Phosphorylation 20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q8NHV4 516 S 18669648 HTP Phosphorylation Q8NHV4 411 S 18669648 HTP Phosphorylation Q8NHV4 423 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q8NHV4 325 Phosphoserine 23186163 Q8NHV4 382 Phosphothreonine; by PLK1 19509060 Q8NHV4 397 Phosphoserine; by PLK1 23186163;19509060 Q8NHV4 411 Phosphoserine 18691976;23186163 Q8NHV4 426 Phosphoserine; by PLK1 19509060 Q8NHV4 468 Phosphoserine 23186163 Q8NHV4 516 Phosphoserine 18669648;19690332;23186163 Q8NHV4 550 Phosphothreonine; by CDK1 19509060 Q8NHV4 637 Phosphoserine; by PLK1 19509060

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type NEDD1 IPI00169345 S 114 SKRVHRpSLKDHKD phosphorylation NEDD1 IPI00169345 S 397 KNQDFSpSFDDTGK phosphorylation NEDD1 IPI00169345 S 411 SLGDMFpSPIRDDA phosphorylation NEDD1 IPI00169345 S 516 SGNLNTpSPSSNQT phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 121441 125728 - K Ubiquitination 21139048 121441 125728 - K Ubiquitination 21906983

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID NEDD1 Homo sapiens (Human) 391; 243; 570 "391,KETDSGKNQDFSS; 243,SSKKLVKTLVADT; 570,ASSLSEKIADSIG" 391:21906983; 243:21906983; 570:21906983 NEDD1 Homo sapiens (Human) 577; 250; 398 "577,ASSLSEKIADSIG; 250,SSKKLVKTLVADT; 398,KETDSGKNQDFSS" 577:21906983; 250:21906983; 398:21906983 NEDD1 Homo sapiens (Human) 391; 243; 570 "391,KETDSGKNQDFSS; 243,SSKKLVKTLVADT; 570,ASSLSEKIADSIG" 391:21906983; 243:21906983; 570:21906983