PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus UBE2U TRIM65 19690564 biogrid,intact exp P P P P P P P P P P P P P P P P P P P GP TRIM65 RBCK1 22493164 biogrid,intact exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G ELAVL1 TRIM65 19322201 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP G GP GP G TRIM65 CCNJL 26186194 biogrid exp GP GP G G GP G GP GP G GP G GP GP GP GP G GP G GP P GP G GP G GP GP G TRIM65 SRPK1 23602568 biogrid,intact exp GP GP G G G GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G TRIM65 ANAPC11 22493164 biogrid,intact exp GP GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G ALDH3B1 TRIM65 26186194 biogrid exp GP GP G G GP GP G GP GP G GP GP GP G G GP GP G GP G GP GP G G GP GP GP GP G MKRN3 TRIM65 22493164 biogrid,intact exp P P P P P P P P P P P P P P P P P P TRIM65 TNRC6B 24778252 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G TRIM65 TNRC6A 24778252 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G TRIM65 MARCH7 22493164 biogrid,intact exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP G GP G GP GP GP G GP G GP GP G TRIM65 UBE2Z 19549727 biogrid,intact exp GP GP G G GP GP G G GP G GP GP GP GP G GP GP G GP G GP GP GP G GP GP GP GP G ARHGAP25 TRIM65 26186194 biogrid exp G G GP P P GP G GP GP G P GP G G GP GP P G UBE3A TRIM65 21900206 biogrid,mint,intact exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G TRIM65 TRIM55 23023127 pred P P GP P P GP P P P P P P G GP P GP TRIM65 TRIM43 23023127 pred P P P P P P P P P P P P P P P P P TRIM65 RNF39 23023127 pred G GP G G GP GP G GP GP G GP G GP G G GP GP GP GP G GP G G G GP G GP GP G TRIM65 RNF39 23023127 pred G GP G G GP GP G GP GP G GP G GP G G GP GP GP GP G GP G G G GP G GP GP G TRIM65 TRIM17 23023127 pred P P P P P P P P P P P P P P P P P GP TRIM65 TRIM54 23023127 pred P P P GP P P P P G P G GP P P UBE2N TRIM65 23023127 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP G GP G GP GP GP G GP GP GP GP G UBE2NL TRIM65 23023127 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G TRIM65 TRIM4 23023127 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G TRIM16L TRIM65 23023127 pred G GP G G G G GP GP G G G G G BSPRY TRIM65 23023127 pred G GP G G G GP G G GP G G G GP G G G GP G G G GP G GP G G G GP G TRIM65 BTNL3 23023127 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G CALM1, CALM2, CALM3 TRIM65 23023127 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G RFPL3 TRIM65 23023127 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G RFPL4A TRIM65 23023127 pred P P P P P P P P P P P P P P P P P P P P P TRIM65 MID2 23023127 pred GP GP G G G GP G GP GP G GP GP GP G G GP GP GP GP G GP G GP G GP G GP GP G TRIM65 TRIM63 23023127 pred G G G G UBE2B TRIM65 23023127 pred G G G G G G G G G G G G G G G G G G G G G G G G G G G G G TRIM65 TRIM50 23023127 pred P P P P P GP P P P P P GP P P P P P GP GP TRIM28 TRIM65 23023127 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G MEFV TRIM65 23023127 pred G G G G G G G G G G G G G G G G G G G G TRIM65 TRIM33 23023127 pred GP GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G TRIM65 TRIML1 23023127 pred P P P P P P P P P P P P P P GP TRIM65 TRIM10 23023127 pred G G G G G G G G G G G G G G GP G GP G G G G G G GP GP GP G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q9H992 Q6PJ69 two hybrid 22493164 direct interaction X 2 Q9H832 Q6PJ69 two hybrid 19549727 direct interaction X 2 P0CG48 Q6PJ69 affinity chromatography technology 22505724 physical association X 2 P0CG48 Q6PJ69 affinity chromatography technology 21963094 physical association X 2 P0CG48 Q6PJ69 affinity chromatography technology 25147182 physical association X 2 P0CG48 Q6PJ69 affinity chromatography technology 21890473 physical association X 2 P0CG48 Q6PJ69 affinity chromatography technology 21906983 physical association X 2 Q13064 Q6PJ69 two hybrid 22493164 direct interaction X 2 Q6PJ69 Q9NYG5 two hybrid 22493164 direct interaction X 2 Q6PJ69 Q9UPQ9-1 affinity chromatography technology 24778252 physical association X 2 Q6PJ69 two hybrid 19690564 direct interaction X 2 Q96SB4 Q6PJ69 enzymatic study 23602568 direct interaction X 2 Q8NDV7 Q6PJ69 affinity chromatography technology 24778252 physical association X 2 Q8NDV7 Q6PJ69 affinity chromatography technology 24778252 physical association X 2 Q8NDV7 Q6PJ69 enzymatic study 24778252 direct interaction X 2 Q6PJ69 two hybrid 22493164 direct interaction X 2 Q6PJ69 Q05086 two hybrid 21900206 direct interaction X 2 Q7CGS9 Q6PJ69 two hybrid pooling approach 20711500 physical association X 2 Q9H992 Q6PJ69 two hybrid array 22493164 physical association X 2 Q9H832 Q6PJ69 two hybrid 19549727 physical association X 2 Q13064 Q6PJ69 two hybrid array 22493164 physical association X 2 Q6PJ69 Q9NYG5 two hybrid array 22493164 physical association X 2 Q96SB4 Q6PJ69 protein kinase assay 23602568 phosphorylation reaction X 2 Q9BYM8 Q6PJ69 two hybrid array 22493164 physical association X 2 Q6PJ69 Q5VVX9 two hybrid array 19690564 physical association X 2 Q6PJ69 Q05086 two hybrid 21900206 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B TRIM65 RBCK1 MI:0415(enzymatic study)|MI:0397(two hybrid array) 22493164 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_284089|UniProt_122533 prey|bait bait|prey ELAVL1 TRIM65 MI:0096(pull down) 19322201 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_187909 bait prey TRIM65 ANAPC11 MI:0397(two hybrid array)|MI:0415(enzymatic study) 22493164 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction) (intact)|(biogrid) UniProt_122554|BIOGRID_284077 bait|prey prey|bait UBE3A TRIM65 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 21900206 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction) (intact)|(intact)|(biogrid) UniProt_178385|UniProt_157787|BIOGRID_156551 bait|bait|bait prey|prey|prey UBE2U TRIM65 MI:0018(two hybrid)|MI:0397(two hybrid array) 19690564 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_123685|UniProt_102674 bait|bait prey|prey TRIM65 UBE2Z MI:0018(two hybrid) 19549727 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (intact) UniProt_100951 prey bait TRIM65 MARCH7 MI:0415(enzymatic study)|MI:0397(two hybrid array) 22493164 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_284081|UniProt_122575 prey|bait bait|prey MKRN3 TRIM65 MI:0397(two hybrid array)|MI:0415(enzymatic study) 22493164 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction) (intact)|(biogrid) UniProt_122614|BIOGRID_284093 prey|prey bait|bait UBC TRIM65 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 21890473,21906983,21963094,22505724 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid)|(biogrid)|(biogrid) BIOGRID_266123|BIOGRID_223646|BIOGRID_179388|BIOGRID_174713 bait|bait|bait|bait prey|prey|prey|prey TRIM65 SRPK1 MI:0004(affinity chromatography technology)|MI:0676(tandem affinity purification) 23602568 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0217(phosphorylation reaction) (biogrid)|(intact) BIOGRID_291575|UniProt_129120 prey|neutral component bait|neutral component

PPI - HINT Gene A Gene B PMID Method Quality ALDH3B1 TRIM65 26186194 0004 HT TRIM65 CCNJL 26186194 0004 HT TRIM65 HEL-S-308 26186194 0004 HT ALDH3B1 TRIM65 26186194 0004 HT TRIM65 CCNJL 26186194 0004 HT TRIM65 HEL-S-308 26186194 0004 HT UBE3A TRIM65 21900206 0018 HT MKRN3 TRIM65 22493164,22493164 0018,0397 HT UBE2U TRIM65 19690564,19690564 0397,0018 HT TRIM65 TNRC6A 24778252,24778252 0004,0415 LC TRIM65 SRPK1 23602568,23602568 0415,0424 LC TRIM65 RBCK1 22493164,22493164 0018,0397 HT TRIM65 UBE2Z 19549727 0018 HT TRIM65 MARCH7 22493164,22493164 0018,0397 HT TRIM65 ANAPC11 22493164,22493164 0018,0397 HT

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q8NDV7 Q6PJ69 TNRC6A TRIM65 0.376 24778252 Q9NYG5 Q6PJ69 ANAPC11 TRIM65 0.286 22493164 Q8IV13 Q6PJ69 CCNJL TRIM65 0.126 26186194 Q6PJ69 Q5VVX9 TRIM65 UBE2U 0.376 19690564 Q7CGS9 Q6PJ69 PEPP TRIM65 0.126 20711500 Q9H832 Q6PJ69 UBE2Z TRIM65 0.454 19549727 Q6PJ69 P04637 TRIM65 TP53 0.21 27012201 Q9H992 Q6PJ69 MARCH7 TRIM65 0.286 22493164 Q96SB4 Q6PJ69 SRPK1 TRIM65 0.21 23602568 Q9BYM8 Q6PJ69 RBCK1 TRIM65 0.286 22493164 Q9UPQ9 Q6PJ69 TNRC6B TRIM65 0.126 24778252 Q6PJ69 P43353 TRIM65 ALDH3B1 0.126 26186194 Q6PJ69 Q13064 TRIM65 MKRN3 0.286 22493164 Q6PJ69 P42331 TRIM65 ARHGAP25 0.126 26186194 Q6PJ69 Q15717 TRIM65 ELAVL1 0.126 19322201 Q6PJ69 Q2MG95 TRIM65 BVLF1 {ECO:0000313|EMBL:CAJ75461.1} 0.126 22810586 Q6PJ69 Q05086 TRIM65 UBE3A 0.332 21900206

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 UBC TRIM65 MI:0045 experimental interaction detection MI:0461 interaction database ARHGAP25 TRIM65 MI:0045 experimental interaction detection MI:0461 interaction database ALDH3B1 TRIM65 MI:0045 experimental interaction detection MI:0461 interaction database UBE3A TRIM65 MI:0045 experimental interaction detection MI:0461 interaction database MKRN3 TRIM65 MI:0045 experimental interaction detection MI:0461 interaction database ELAVL1 TRIM65 MI:0045 experimental interaction detection MI:0461 interaction database UBE2U TRIM65 MI:0045 experimental interaction detection MI:0461 interaction database TRIM65 CCNJL MI:0045 experimental interaction detection MI:0461 interaction database TRIM65 TNRC6A MI:0045 experimental interaction detection MI:0461 interaction database TRIM65 SRPK1 MI:0045 experimental interaction detection MI:0461 interaction database TRIM65 RBCK1 MI:0045 experimental interaction detection MI:0461 interaction database TRIM65 UBE2Z MI:0045 experimental interaction detection MI:0461 interaction database TRIM65 MARCH7 MI:0045 experimental interaction detection MI:0461 interaction database TRIM65 ANAPC11 MI:0045 experimental interaction detection MI:0461 interaction database TRIM65 TNRC6B MI:0045 experimental interaction detection MI:0461 interaction database