PTM - CPLM Gene Name UniProt ID Type Position Code CBX4 O00257 Acetylation 149 K CBX4 O00257 Sumoylation 494 K CBX4 O00257 Ubiquitination 278 K CBX4 O00257 Ubiquitination 38 K CBX4 O00257 Ubiquitination 212 K CBX4 O00257 Ubiquitination 280 K CBX4 O00257 Ubiquitination 114 K CBX4 O00257 Ubiquitination 178 K CBX4 O00257 Ubiquitination 249 K CBX4 O00257 Ubiquitination 365 K

PTM - dbPAF Gene Name UniProt ID Position Type Species CBX4 O00257 120 Y Homo sapiens(Human) CBX4 O00257 150 Y Homo sapiens(Human) CBX4 O00257 151 Y Homo sapiens(Human) CBX4 O00257 152 Y Homo sapiens(Human) CBX4 O00257 169 Y Homo sapiens(Human) CBX4 O00257 182 S Homo sapiens(Human) CBX4 O00257 207 Y Homo sapiens(Human) CBX4 O00257 267 S Homo sapiens(Human) CBX4 O00257 28 Y Homo sapiens(Human) CBX4 O00257 291 S Homo sapiens(Human) CBX4 O00257 293 S Homo sapiens(Human) CBX4 O00257 336 S Homo sapiens(Human) CBX4 O00257 345 S Homo sapiens(Human) CBX4 O00257 349 S Homo sapiens(Human) CBX4 O00257 351 T Homo sapiens(Human) CBX4 O00257 415 S Homo sapiens(Human) CBX4 O00257 417 T Homo sapiens(Human) CBX4 O00257 429 T Homo sapiens(Human) CBX4 O00257 432 S Homo sapiens(Human) CBX4 O00257 434 S Homo sapiens(Human) CBX4 O00257 437 T Homo sapiens(Human) CBX4 O00257 442 S Homo sapiens(Human) CBX4 O00257 467 S Homo sapiens(Human) CBX4 O00257 497 T Homo sapiens(Human) CBX4 O00257 90 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA O00257 K149-ac Acetylation PPPGKSGkyyyQLNS O00257 K157-ac Acetylation yyyQLNSkKHHPYQP O00257 R137-m1 Methylation YQPHSKErAGKPPPP O00257 K249-m1 Methylation TGNGIGGkMKIVKNK O00257 Y28-p Phosphorylation IRKGRVEyLVKWRGW O00257 S90-p Phosphorylation VPTFARRsNVLTGLQ O00257 Y120-p Phosphorylation GkGQGHQyELNSKKH O00257 Y150-p Phosphorylation PPGKSGkyyyQLNSk O00257 Y151-p Phosphorylation PGKSGkyyyQLNSkK O00257 Y152-p Phosphorylation GKSGkyyyQLNSkKH O00257 Y169-p Phosphorylation YQPDPKMyDLQYQGG O00257 S182-p Phosphorylation GGHKEAPsPtCPDLG O00257 T184-p Phosphorylation HKEAPsPtCPDLGAK O00257 S291-p Phosphorylation VAEGEARsPsHKKRA O00257 S293-p Phosphorylation EGEARsPsHKKRAAD O00257 S336-p Phosphorylation EEEVSGVsDPQPQDA O00257 S345-p Phosphorylation PQPQDAGsRKLsPTK O00257 S349-p Phosphorylation DAGsRKLsPTKEAFG O00257 S415-p Phosphorylation HVRKRCLsEtHGERE O00257 T417-p Phosphorylation RKRCLsEtHGEREPC O00257 S432-p Phosphorylation KKRLTARsIsTPtCL O00257 S434-p Phosphorylation RLTARsIsTPtCLGG O00257 T437-p Phosphorylation ARsIsTPtCLGGsPA O00257 S442-p Phosphorylation TPtCLGGsPAAERPA O00257 S467-p Phosphorylation PEVILLDsDLDEPID O00257 T497-p Phosphorylation SLQVkPEtPASAAVA O00257 K106-sm Sumoylation SSTDNRAkLDLGAQG O00257 K114-sm Sumoylation LDLGAQGkGQGHQyE O00257 K149-sm Sumoylation PPPGKSGkyyyQLNS O00257 K205-sm Sumoylation WAQGAGAkGYLGAVk O00257 K212-sm Sumoylation kGYLGAVkPLAGAAG O00257 K223-sm Sumoylation GAAGAPGkGSEKGPP O00257 K249-sm Sumoylation TGNGIGGkMKIVKNK O00257 K278-sm Sumoylation ENGMQAVkIkSGEVA O00257 K280-sm Sumoylation GMQAVkIkSGEVAEG O00257 K365-sm Sumoylation QPLQLTTkPDLLAWD O00257 K494-sm Sumoylation PPSSLQVkPEtPASA O00257 K212-ub Ubiquitination kGYLGAVkPLAGAAG O00257 K365-ub Ubiquitination QPLQLTTkPDLLAWD

PTM - dbPTM UniProt ID Position Code Type Resources References O00257 90 S Phosphorylation Swiss-Prot 1010711 19007248 O00257 149 K Acetylation Swiss-Prot 1010711 19608861 O00257 494 K Sumoylation Swiss-Prot 1010711 15592428 O00257 497 T Phosphorylation PhosphoELM.10011 17018294 O00257 494 K Sumoylation Phosphositeplus.1010730 15175327;17018294 O00257 365 K Ubiquitylation Phosphositeplus.1010730 21906983 O00257 149 K Acetylation Phosphositeplus.1010730 19608861 O00257 182 S Phosphorylation Phosphositeplus.1010730 19691289 O00257 336 S Phosphorylation Phosphositeplus.1010730 20044836 O00257 349 S Phosphorylation Phosphositeplus.1010730 20068231 O00257 432 S Phosphorylation Phosphositeplus.1010730 18220336 O00257 417 T Phosphorylation Phosphositeplus.1010730 19276368 O00257 437 T Phosphorylation Phosphositeplus.1010730 19276368 O00257 497 T Phosphorylation Phosphositeplus.1010730 17018294 O00257 207 Y Phosphorylation Phosphositeplus.1010730 18083107 O00257 149 K Acetylation HPRD 9.0 19608861 O00257 90 S Phosphorylation HPRD 9.0 19007248 O00257 182 S Phosphorylation HPRD 9.0 19413330,19691289 O00257 267 S Phosphorylation HPRD 9.0 20068231 O00257 293 S Phosphorylation HPRD 9.0 17081983 O00257 349 S Phosphorylation HPRD 9.0 18669648,20068231 O00257 415 S Phosphorylation HPRD 9.0 17081983 O00257 351 T Phosphorylation HPRD 9.0 18669648 O00257 293 S Phosphorylation SysPTM 1.1 17081983 O00257 415 S Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References CBX4 149 K Acetylation 19608861 CBX4 182 S Phosphorylation 19413330,19691289 CBX4 267 S Phosphorylation 20068231 CBX4 293 S Phosphorylation 17081983 CBX4 349 S Phosphorylation 18669648,20068231 CBX4 351 T Phosphorylation 18669648 CBX4 415 S Phosphorylation 17081983 CBX4 90 S Phosphorylation 19007248

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type O00257 495 T 17018294 HIPK2 LTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs O00257 149 N6-acetyllysine; alternate 19608861 O00257 182 Phosphoserine 23186163 O00257 467 Phosphoserine 24275569 O00257 497 Phosphothreonine; by HIPK2 17018294

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type CBX4 IPI00010872 K 149 PPGKSGaKYYYQLN acetylation CBX4 IPI00010872 S 349 AGSRKLpSPTKEAF phosphorylation CBX4 IPI00010872 S 415 VRKRCLpSETHGER phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 8535 114105 - K Ubiquitination 18781797 8535 114105 - K Sumoylation 21693764 8535 114105 - K Ubiquitination 21906983 8535 114105 - K Sumoylation 19471022 8535 114105 - K Sumoylation 21252943 8535 114105 - K Sumoylation 22467880 8535 114105 - K Sumoylation 15175327 8535 114105 - K FAT10ylation 22797925 8535 114105 - K Ubiquitination 23000965 8535 114105 - K Neddylation 24654937 8535 114105 - K Sumoylation 24654937 8535 114105 - K Sumoylation 25391492

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID CBX4 Homo sapiens (Human) 365 "365,PLQLTTKPDLLAW" 365:21906983