PTM - CPLM Gene Name UniProt ID Type Position Code SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 32 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 53 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 217 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 335 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 208 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 45 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 195 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 230 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 198 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 209 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 141 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 228 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 180 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 210 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 61 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 183 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 148 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 152 K SAE1; AOS1; SUA1; UBLE1A Q9UBE0 Ubiquitination 109 K

PTM - dbPAF Gene Name UniProt ID Position Type Species SAE1; AOS1; SUA1; UBLE1A Q9UBE0 12 S Homo sapiens(Human) SAE1; AOS1; SUA1; UBLE1A Q9UBE0 185 S Homo sapiens(Human) SAE1; AOS1; SUA1; UBLE1A Q9UBE0 194 T Homo sapiens(Human) SAE1; AOS1; SUA1; UBLE1A Q9UBE0 201 S Homo sapiens(Human) SAE1; AOS1; SUA1; UBLE1A Q9UBE0 238 S Homo sapiens(Human) SAE1; AOS1; SUA1; UBLE1A Q9UBE0 240 Y Homo sapiens(Human) SAE1; AOS1; SUA1; UBLE1A Q9UBE0 262 Y Homo sapiens(Human) SAE1; AOS1; SUA1; UBLE1A Q9UBE0 266 S Homo sapiens(Human) SAE1; AOS1; SUA1; UBLE1A Q9UBE0 37 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9UBE0 K195-ac Acetylation VEDGPdTkRAkLDsS Q9UBE0 K198-ac Acetylation GPdTkRAkLDsSETT Q9UBE0 K228-ac Acetylation EVDWSSEkAKAALKR Q9UBE0 R24-m1 Methylation AQYDRQIrLWGLEAQ Q9UBE0 S12-p Phosphorylation EEAGGGIsEEEAAQY Q9UBE0 S37-p Phosphorylation AQkRLRAsRVLLVGL Q9UBE0 S150-p Phosphorylation DQICHkNsIKFFTGD Q9UBE0 S185-p Phosphorylation KTKVAkVsQGVEDGP Q9UBE0 S201-p Phosphorylation TkRAkLDsSETTMVk Q9UBE0 Y262-p Phosphorylation RDPSSDTyEEDsELL Q9UBE0 S266-p Phosphorylation SDTyEEDsELLLQIR Q9UBE0 K109-sm Sumoylation LNPMVDVkVDTEDIE Q9UBE0 K32-ub Ubiquitination LWGLEAQkRLRAsRV Q9UBE0 K45-ub Ubiquitination RVLLVGLkGLGAEIA Q9UBE0 K53-ub Ubiquitination GLGAEIAkNLILAGV Q9UBE0 K61-ub Ubiquitination NLILAGVkGLTMLDH Q9UBE0 K109-ub Ubiquitination LNPMVDVkVDTEDIE Q9UBE0 K148-ub Ubiquitination KVDQICHkNsIKFFT Q9UBE0 K183-ub Ubiquitination EEKTKVAkVsQGVED Q9UBE0 K195-ub Ubiquitination VEDGPdTkRAkLDsS Q9UBE0 K198-ub Ubiquitination GPdTkRAkLDsSETT Q9UBE0 K208-ub Ubiquitination sSETTMVkKKVVFCP Q9UBE0 K228-ub Ubiquitination EVDWSSEkAKAALKR Q9UBE0 K335-ub Ubiquitination FFFFDGMkGNGIVEC

PTM - dbPTM UniProt ID Position Code Type Resources References Q9UBE0 1 M Acetylation Swiss-Prot 1010711 19413330 Q9UBE0 185 S Phosphorylation Swiss-Prot 1010711 17525332 Q9UBE0 185 S Phosphorylation PhosphoELM.10011 17525332 Q9UBE0 193 D Caspase Phosphositeplus.1010730 18724940 Q9UBE0 109 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UBE0 183 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UBE0 195 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UBE0 208 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UBE0 228 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UBE0 32 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UBE0 45 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UBE0 53 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UBE0 61 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UBE0 12 S Phosphorylation Phosphositeplus.1010730 19664994;21659604 Q9UBE0 185 S Phosphorylation Phosphositeplus.1010730 17525332;18077418;20393185 Q9UBE0 12 S Phosphorylation HPRD 9.0 19664994 Q9UBE0 185 S Phosphorylation HPRD 9.0 17525332,18077418

PTM - HPRD Symbol Position Code Type References SAE1 12 S Phosphorylation 19664994 SAE1 185 S Phosphorylation 17525332,18077418

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9UBE0 185 S 17525332 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9UBE0 1 N-acetylmethionine 19413330;22814378 Q9UBE0 12 Phosphoserine 23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type SAE1 IPI00033130 S 201 KRAKLDpSSETTMV phosphorylation SAE1 IPI00033130 T 194 VEDGPDpTKRAKLD phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 10055 115366 - K Ubiquitination 18781797 10055 115366 - K Ubiquitination 21987572 10055 115366 - K Ubiquitination 21139048 10055 115366 - K Ubiquitination 21890473 10055 115366 - K Ubiquitination 21906983 10055 115366 - K Sumoylation 19471022 10055 115366 - K Ubiquitination 22505724 10055 115366 - K Ubiquitination 21963094 10055 115366 - K Ubiquitination 23000965 10055 115366 - K Ubiquitination 24816145 10055 115366 - K Sumoylation 25027693

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID SAE1 Homo sapiens (Human) 32; 53; 109; 228; 208; 183; 61; 195; 45 "32,WGLEAQKRLRASR; 53,LGAEIAKNLILAG; 109,NPMVDVKVDTEDI; 228,VDWSSEKAKAALK; 208,SETTMVKKKVVFC; 183,EKTKVAKVSQGVE; 61,LILAGVKGLTMLD; 195,EDGPDTKRAKLDS; 45,VLLVGLKGLGAEI" "32:21890473,21906983; 53:21890473,21906983; 109:21906983; 228:21906983; 208:21906983; 183:21906983; 61:21906983; 195:21906983; 45:21906983" SAE1 Homo sapiens (Human) 32; 53 "32,WGLEAQKRLRASR; 53,LGAEIAKNLILAG" 32:21890473; 53:21890473 SAE1 Homo sapiens (Human) 32; 53; 109; 228; 208; 183; 61; 195; 45 "32,WGLEAQKRLRASR; 53,LGAEIAKNLILAG; 109,NPMVDVKVDTEDI; 228,VDWSSEKAKAALK; 208,SETTMVKKKVVFC; 183,EKTKVAKVSQGVE; 61,LILAGVKGLTMLD; 195,EDGPDTKRAKLDS; 45,VLLVGLKGLGAEI" "32:21906983,21890473; 53:21906983,21890473; 109:21906983; 228:21906983; 208:21906983; 183:21906983; 61:21906983; 195:21906983; 45:21906983" SAE1 Homo sapiens (Human) 32; 53 "32,WGLEAQKRLRASR; 53,LGAEIAKNLILAG" 32:21890473; 53:21890473