PTM - CPLM Gene Name UniProt ID Type Position Code BUB3 O43684 Acetylation 179 K BUB3 O43684 Ubiquitination 216 K BUB3 O43684 Ubiquitination 191 K BUB3 O43684 Ubiquitination 52 K BUB3 O43684 Ubiquitination 322 K BUB3 O43684 Ubiquitination 139 K BUB3 O43684 Ubiquitination 179 K BUB3 O43684 Ubiquitination 8 K BUB3 O43684 Ubiquitination 217 K BUB3 O43684 Ubiquitination 218 K BUB3 O43684 Ubiquitination 324 K BUB3 O43684 Ubiquitination 222 K BUB3 O43684 Ubiquitination 21 K

PTM - dbPAF Gene Name UniProt ID Position Type Species BUB3 O43684 127 T Homo sapiens(Human) BUB3 O43684 141 Y Homo sapiens(Human) BUB3 O43684 144 S Homo sapiens(Human) BUB3 O43684 154 T Homo sapiens(Human) BUB3 O43684 19 S Homo sapiens(Human) BUB3 O43684 194 Y Homo sapiens(Human) BUB3 O43684 207 Y Homo sapiens(Human) BUB3 O43684 211 S Homo sapiens(Human) BUB3 O43684 325 S Homo sapiens(Human) BUB3 O43684 328 T Homo sapiens(Human) BUB3 O43684 33 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA O43684 K179-ac Acetylation QRRESSLkYQTRCIr O43684 K216-ac Acetylation DPsPEVQkkKYAFKC O43684 K322-ac Acetylation QVTDAETkPksPCt_ O43684 R126-m1 Methylation TVKLWDPrtPCNAGT O43684 R149-m1 Methylation TLsVSGDrLIVGtAG O43684 R186-m1 Methylation kYQTRCIrAFPNkQG O43684 S19-p Phosphorylation PPEDGISsVkFSPNT O43684 S33-p Phosphorylation TSQFLLVsSWDTSVR O43684 S48-p Phosphorylation LYDVPANsMRLkYQH O43684 T86-p Phosphorylation LKMHDLNtDQENLVG O43684 T127-p Phosphorylation VKLWDPrtPCNAGTF O43684 Y141-p Phosphorylation FSQPEkVyTLsVSGD O43684 S144-p Phosphorylation PEkVyTLsVSGDrLI O43684 T154-p Phosphorylation GDrLIVGtAGRRVLV O43684 Y194-p Phosphorylation AFPNkQGyVLSSIEG O43684 Y207-p Phosphorylation EGRVAVEyLDPsPEV O43684 S211-p Phosphorylation AVEyLDPsPEVQkkK O43684 S325-p Phosphorylation DAETkPksPCt____ O43684 T328-p Phosphorylation TkPksPCt_______ O43684 K8-sm Sumoylation MTGSNEFkLNQPPED O43684 K322-sm Sumoylation QVTDAETkPksPCt_ O43684 K8-ub Ubiquitination MTGSNEFkLNQPPED O43684 K21-ub Ubiquitination EDGISsVkFSPNTSQ O43684 K52-ub Ubiquitination PANsMRLkYQHTGAV O43684 K139-ub Ubiquitination GTFSQPEkVyTLsVS O43684 K179-ub Ubiquitination QRRESSLkYQTRCIr O43684 K191-ub Ubiquitination CIrAFPNkQGyVLSS O43684 K216-ub Ubiquitination DPsPEVQkkKYAFKC O43684 K217-ub Ubiquitination PsPEVQkkKYAFKCH O43684 K322-ub Ubiquitination QVTDAETkPksPCt_ O43684 K324-ub Ubiquitination TDAETkPksPCt___

PTM - dbPTM UniProt ID Position Code Type Resources References O43684 179 K Acetylation Swiss-Prot 1010711 19608861 O43684 211 S Phosphorylation Swiss-Prot 1010711 15302935;18669648 O43684 216 K Ubiquitylation Swiss-Prot 1010711 18781797 O43684 211 S Phosphorylation PhosphoELM.10011 22067460;18669648;15302935 O43684 325 S Phosphorylation PhosphoELM.10011 22067460 O43684 191 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 O43684 21 K Ubiquitylation Phosphositeplus.1010730 21906983 O43684 216 K Ubiquitylation Phosphositeplus.1010730 18781797;21906983 O43684 322 K Ubiquitylation Phosphositeplus.1010730 21906983 O43684 179 K Acetylation Phosphositeplus.1010730 19608861 O43684 19 S Phosphorylation Phosphositeplus.1010730 20057499 O43684 211 S Phosphorylation Phosphositeplus.1010730 15302935;18669648;19664994;22067460 O43684 325 S Phosphorylation Phosphositeplus.1010730 22067460 O43684 154 T Phosphorylation Phosphositeplus.1010730 20068230 O43684 328 T Phosphorylation Phosphositeplus.1010730 22067460 O43684 179 K Acetylation HPRD 9.0 19608861 O43684 211 S Phosphorylation HPRD 9.0 15302935,18669648,18452278 O43684 154 T Phosphorylation HPRD 9.0 20068230 O43684 194 Y Phosphorylation HPRD 9.0 17322306 O43684 216 K Ubiquitylation SysPTM 1.1 18781797 O43684 211 S Phosphorylation SysPTM 1.1 18669648

PTM - HPRD Symbol Position Code Type References BUB3 154 T Phosphorylation 20068230 BUB3 179 K Acetylation 19608861 BUB3 194 Y Phosphorylation 17322306 BUB3 211 S Phosphorylation 15302935,18669648,18452278

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type O43684 211 S 22067460 HTP Phosphorylation O43684 211 S 18669648 HTP Phosphorylation O43684 211 S 15302935 HTP Phosphorylation O43684 325 S 22067460 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs O43684 179 N6-acetyllysine 19608861 O43684 211 Phosphoserine 18669648;23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type BUB3 IPI00013468 K 179 RRESSLaKYQTRCI acetylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 9184 114621 - K Ubiquitination 21890473 9184 114621 - K Ubiquitination 18781797 9184 114621 - K Ubiquitination 21987572 9184 114621 - K Ubiquitination 21963094 9184 114621 - K Ubiquitination 21139048 9184 114621 - K Ubiquitination 21906983 9184 114621 - K Sumoylation 21110914 9184 114621 - K Ubiquitination 22053931 9184 114621 - K Ubiquitination 21853274 9184 114621 - K Ubiquitination 22505724 9184 114621 - K Ubiquitination 23000965 9184 114621 - K Ubiquitination 24654937 9184 114621 - K Neddylation 24654937 9184 114621 - K Sumoylation 24654937 9184 114621 - K Ubiquitination 24816145 9184 114621 - K Sumoylation 25027693 9184 114621 - K Ubiquitination 25015289 9184 114621 - K Ubiquitination 25147182 9184 114621 - K Sumoylation 25391492

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID BUB3 Homo sapiens (Human) 191 "191,IRAFPNKQGYVLS" 191:21890473 BUB3 Homo sapiens (Human) 191 "191,IRAFPNKQGYVLS" 191:21906983 BUB3 Homo sapiens (Human) 21 "21,DGISSVKFSPNTS" 21:21906983 BUB3 Homo sapiens (Human) 139; 21; 216; 322; 191; 52 "139,TFSQPEKVYTLSV; 21,DGISSVKFSPNTS; 216,PSPEVQKKKYAFK; 322,VTDAETKPKSPCT; 191,IRAFPNKQGYVLS; 52,ANSMRLKYQHTGA" "139:22053931; 21:21906983; 216:18781797,21906983,21139048; 322:21906983,22053931; 191:21890473,21906983; 52:22053931" BUB3 Homo sapiens (Human) 191 "191,IRAFPNKQGYVLS" 191:21890473 BUB3 Homo sapiens (Human) 191 "191,IRAFPNKQGYVLS" 191:21890473