PTM - CPLM Gene Name UniProt ID Type Position Code RABGEF1; RABEX5 Q9UJ41 Acetylation 329 K RABGEF1; RABEX5 Q9UJ41 Acetylation 348 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 281 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 263 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 318 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 325 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 329 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 348 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 423 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 520 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 629 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 299 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 336 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 268 K RABGEF1; RABEX5 Q9UJ41 Ubiquitination 650 K

PTM - dbPAF Gene Name UniProt ID Position Type Species RABGEF1; RABEX5 Q9UJ41 284 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 291 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 302 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 309 T Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 310 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 313 T Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 562 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 590 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 594 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 601 Y Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 603 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 606 T Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 607 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 615 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 617 S Homo sapiens(Human) RABGEF1; RABEX5 Q9UJ41 673 Y Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9UJ41 K329-ac Acetylation EFLkTFHkTGQEIYK Q9UJ41 K348-ac Acetylation FLEGMHYkRDLSIEE Q9UJ41 K430-ac Acetylation kIMDQIEkYIMTRLY Q9UJ41 T5-p Phosphorylation ___MVVVtGREPDSR Q9UJ41 S284-p Phosphorylation TTVkkFFsAsSRVGs Q9UJ41 S286-p Phosphorylation VkkFFsAsSRVGsKK Q9UJ41 S291-p Phosphorylation sAsSRVGsKKEIQEA Q9UJ41 S302-p Phosphorylation IQEAkAPsPSINRQt Q9UJ41 T309-p Phosphorylation sPSINRQtsIEtDRV Q9UJ41 S310-p Phosphorylation PSINRQtsIEtDRVS Q9UJ41 T313-p Phosphorylation NRQtsIEtDRVSkEF Q9UJ41 S562-p Phosphorylation ITRFCNPsRLMTGED Q9UJ41 S590-p Phosphorylation IEKLDAQsLNLsQED Q9UJ41 S594-p Phosphorylation DAQsLNLsQEDFDRY Q9UJ41 S603-p Phosphorylation EDFDRYMsGQtsPRK Q9UJ41 T606-p Phosphorylation DRYMsGQtsPRKQEA Q9UJ41 S607-p Phosphorylation RYMsGQtsPRKQEAE Q9UJ41 S615-p Phosphorylation PRKQEAEsWsPDACL Q9UJ41 S617-p Phosphorylation KQEAEsWsPDACLGV Q9UJ41 Y673-p Phosphorylation VQDIVEKyPLEIKPP Q9UJ41 K520-sm Sumoylation KHIFNAIkITKNEPA Q9UJ41 K263-ub Ubiquitination AQSLTFSkFEEkkTN Q9UJ41 K267-ub Ubiquitination TFSkFEEkkTNEKTR Q9UJ41 K268-ub Ubiquitination FSkFEEkkTNEKTRK Q9UJ41 K280-ub Ubiquitination TRKVTTVkkFFsAsS Q9UJ41 K281-ub Ubiquitination RKVTTVkkFFsAsSR Q9UJ41 K299-ub Ubiquitination KKEIQEAkAPsPSIN Q9UJ41 K318-ub Ubiquitination IEtDRVSkEFIEFLk Q9UJ41 K325-ub Ubiquitination kEFIEFLkTFHkTGQ Q9UJ41 K329-ub Ubiquitination EFLkTFHkTGQEIYK Q9UJ41 K339-ub Ubiquitination QEIYKQTkLFLEGMH Q9UJ41 K348-ub Ubiquitination FLEGMHYkRDLSIEE Q9UJ41 K423-ub Ubiquitination VPPERVEkIMDQIEk Q9UJ41 K520-ub Ubiquitination KHIFNAIkITKNEPA Q9UJ41 K629-ub Ubiquitination LGVKQMYkNLDLLSQ

PTM - dbPTM UniProt ID Position Code Type Resources References Q9UJ41 302 S Phosphorylation Swiss-Prot 1010711 18669648;18691976 Q9UJ41 310 S Phosphorylation Swiss-Prot 1010711 18088087 Q9UJ41 329 K Acetylation Swiss-Prot 1010711 19608861 Q9UJ41 348 K Acetylation Swiss-Prot 1010711 19608861 Q9UJ41 617 S Phosphorylation Swiss-Prot 1010711 19413330 Q9UJ41 302 S Phosphorylation PhosphoELM.10011 18669648 Q9UJ41 310 S Phosphorylation PhosphoELM.10011 18088087 Q9UJ41 603 S Phosphorylation PhosphoELM.10011 18669648 Q9UJ41 607 S Phosphorylation PhosphoELM.10011 18669648 Q9UJ41 606 T Phosphorylation PhosphoELM.10011 18669648 Q9UJ41 601 Y Phosphorylation PhosphoELM.10011 18669648 Q9UJ41 263 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UJ41 267 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJ41 280 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJ41 281 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UJ41 299 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJ41 318 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UJ41 325 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UJ41 329 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UJ41 339 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UJ41 348 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UJ41 423 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UJ41 520 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UJ41 629 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UJ41 329 K Acetylation Phosphositeplus.1010730 19608861 Q9UJ41 348 K Acetylation Phosphositeplus.1010730 19608861 Q9UJ41 430 K Acetylation Phosphositeplus.1010730 20167786 Q9UJ41 302 S Phosphorylation Phosphositeplus.1010730 18669648;18691976 Q9UJ41 310 S Phosphorylation Phosphositeplus.1010730 18088087;20363803 Q9UJ41 590 S Phosphorylation Phosphositeplus.1010730 20068231 Q9UJ41 594 S Phosphorylation Phosphositeplus.1010730 20068231 Q9UJ41 603 S Phosphorylation Phosphositeplus.1010730 18669648 Q9UJ41 607 S Phosphorylation Phosphositeplus.1010730 18669648;19690332;20068231 Q9UJ41 313 T Phosphorylation Phosphositeplus.1010730 20068231 Q9UJ41 606 T Phosphorylation Phosphositeplus.1010730 18669648 Q9UJ41 329 K Acetylation HPRD 9.0 19608861 Q9UJ41 348 K Acetylation HPRD 9.0 19608861 Q9UJ41 302 S Phosphorylation HPRD 9.0 18669648,18691976 Q9UJ41 310 S Phosphorylation HPRD 9.0 17081983,18088087,20068231 Q9UJ41 590 S Phosphorylation HPRD 9.0 20068231 Q9UJ41 594 S Phosphorylation HPRD 9.0 20068231 Q9UJ41 603 S Phosphorylation HPRD 9.0 18669648,20068231 Q9UJ41 607 S Phosphorylation HPRD 9.0 18669648,20068231 Q9UJ41 617 S Phosphorylation HPRD 9.0 19413330 Q9UJ41 313 T Phosphorylation HPRD 9.0 20068231 Q9UJ41 606 T Phosphorylation HPRD 9.0 18669648,18691976,20068231 Q9UJ41 601 Y Phosphorylation HPRD 9.0 18669648,18691976 Q9UJ41 310 S Phosphorylation SysPTM 1.1 18088087

PTM - HPRD Symbol Position Code Type References RABGEF1 124 S Phosphorylation 18669648,18691976 RABGEF1 132 S Phosphorylation 17081983,18088087,20068231 RABGEF1 135 T Phosphorylation 20068231 RABGEF1 151 K Acetylation 19608861 RABGEF1 170 K Acetylation 19608861 RABGEF1 302 S Phosphorylation 18669648,18691976 RABGEF1 310 S Phosphorylation 17081983,18088087,20068231 RABGEF1 313 T Phosphorylation 20068231 RABGEF1 329 K Acetylation 19608861 RABGEF1 348 K Acetylation 19608861 RABGEF1 373 S Phosphorylation 20068231 RABGEF1 377 S Phosphorylation 20068231 RABGEF1 384 Y Phosphorylation 18669648,18691976 RABGEF1 386 S Phosphorylation 18669648,20068231 RABGEF1 389 T Phosphorylation 18669648,18691976,20068231 RABGEF1 390 S Phosphorylation 18669648,20068231 RABGEF1 400 S Phosphorylation 19413330 RABGEF1 590 S Phosphorylation 20068231 RABGEF1 594 S Phosphorylation 20068231 RABGEF1 601 Y Phosphorylation 18669648,18691976 RABGEF1 603 S Phosphorylation 18669648,20068231 RABGEF1 606 T Phosphorylation 18669648,18691976,20068231 RABGEF1 607 S Phosphorylation 18669648,20068231 RABGEF1 617 S Phosphorylation 19413330

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9UJ41 606 T 18669648 HTP Phosphorylation Q9UJ41 603 S 18669648 HTP Phosphorylation Q9UJ41 601 Y 18669648 HTP Phosphorylation Q9UJ41 310 S 18088087 HTP Phosphorylation Q9UJ41 302 S 18669648 HTP Phosphorylation Q9UJ41 607 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9UJ41 302 Phosphoserine 18669648;23186163 Q9UJ41 310 Phosphoserine 24275569 Q9UJ41 329 N6-acetyllysine 19608861 Q9UJ41 348 N6-acetyllysine 19608861 Q9UJ41 590 Phosphoserine 20068231 Q9UJ41 594 Phosphoserine 20068231 Q9UJ41 617 Phosphoserine 23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type RABGEF1 IPI00004974 K 151 FLKTFHaKTGQEIY acetylation RABGEF1 IPI00004974 K 170 LEGMHYaKRDLSIE acetylation RABGEF1 IPI00004974 S 124 QEAKAPpSPSINRQ phosphorylation RABGEF1 IPI00004974 S 373 EKLDAQpSLNLSQE phosphorylation RABGEF1 IPI00004974 S 377 AQSLNLpSQEDFDR phosphorylation RABGEF1 IPI00004974 S 390 YMSGQTpSPRKQEA phosphorylation RABGEF1 IPI00004974 T 135 RQTSIEpTDRVSKE phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 27342 118154 - K Ubiquitination 18781797 27342 118154 - K Ubiquitination 21139048 27342 118154 - K Ubiquitination 21890473 27342 118154 - K Ubiquitination 21906983 27342 118154 - K Ubiquitination 22053931 27342 118154 - K Ubiquitination 22505724 27342 118154 - K Ubiquitination 21963094 27342 118154 - K Ubiquitination 23000965 27342 118154 - K Ubiquitination 24654937 27342 118154 - K Ubiquitination 25015289 27342 118154 - K Ubiquitination 25147182 27342 118154 - K Ubiquitination 23048039

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID RABGEF1 Homo sapiens (Human) 102; 170; 103; 206; 140; 412; 161; 85; 147; 89; 121; 151; 303 "102,RKVTTVKKFFSAS; 170,LEGMHYKRDLSIE; 103,KVTTVKKFFSASS; 206,PPERVEKIMDQIE; 140,ETDRVSKEFIEFL; 412,GVKQMYKNLDLLS; 161,EIYKQTKLFLEGM; 85,QSLTFSKFEEKKT; 147,EFIEFLKTFHKTG; 89,FSKFEEKKTNEKT; 121,KEIQEAKAPSPSI; 151,FLKTFHKTGQEIY; 303,HIFNAIKITKNEP" "102:21906983; 170:21890473,21906983; 103:21890473; 206:21890473; 140:21890473,21906983; 412:21890473,21906983; 161:21906983; 85:21890473,21906983,22053931; 147:21890473; 89:21906983; 121:21906983; 151:21890473; 303:21890473,21906983" RABGEF1 Homo sapiens (Human) 339; 281; 348; 280; 520; 318; 325; 263; 629; 267; 299; 423; 329 "339,EIYKQTKLFLEGM; 281,KVTTVKKFFSASS; 348,LEGMHYKRDLSIE; 280,RKVTTVKKFFSAS; 520,HIFNAIKITKNEP; 318,ETDRVSKEFIEFL; 325,EFIEFLKTFHKTG; 263,QSLTFSKFEEKKT; 629,GVKQMYKNLDLLS; 267,FSKFEEKKTNEKT; 299,KEIQEAKAPSPSI; 423,PPERVEKIMDQIE; 329,FLKTFHKTGQEIY" "339:21906983; 281:21890473; 348:21890473,21906983; 280:21906983; 520:21890473,21906983; 318:21890473,21906983; 325:21890473; 263:21890473,21906983; 629:21890473,21906983; 267:21906983; 299:21906983; 423:21890473; 329:21890473" RABGEF1 Homo sapiens (Human) 343; 201; 143; 142; 129; 161; 187; 191; 246; 125; 452; 180; 210 "343,HIFNAIKITKNEP; 201,EIYKQTKLFLEGM; 143,KVTTVKKFFSASS; 142,RKVTTVKKFFSAS; 129,FSKFEEKKTNEKT; 161,KEIQEAKAPSPSI; 187,EFIEFLKTFHKTG; 191,FLKTFHKTGQEIY; 246,PPERVEKIMDQIE; 125,QSLTFSKFEEKKT; 452,GVKQMYKNLDLLS; 180,ETDRVSKEFIEFL; 210,LEGMHYKRDLSIE" "343:21890473,21906983; 201:21906983; 143:21890473; 142:21906983; 129:21906983; 161:21906983; 187:21890473; 191:21890473; 246:21890473; 125:21890473,21906983; 452:21890473,21906983; 180:21890473,21906983; 210:21890473,21906983" RABGEF1 Homo sapiens (Human) 85; 220; 103; 123; 329 "85,QSLTFSKFEEKKT; 220,HIFNAIKITKNEP; 103,KVTTVKKFFSASS; 123,PPERVEKIMDQIE; 329,GVKQMYKNLDLLS" 85:21890473; 220:21890473; 103:21890473; 123:21890473; 329:21890473 RABGEF1 Homo sapiens (Human) 220; 117; 154; 184; 426; 99; 161; 317; 165 "220,PPERVEKIMDQIE; 117,KVTTVKKFFSASS; 154,ETDRVSKEFIEFL; 184,LEGMHYKRDLSIE; 426,GVKQMYKNLDLLS; 99,QSLTFSKFEEKKT; 161,EFIEFLKTFHKTG; 317,HIFNAIKITKNEP; 165,FLKTFHKTGQEIY" 220:21890473; 117:21890473; 154:21890473; 184:21890473; 426:21890473; 99:21890473; 161:21890473; 317:21890473; 165:21890473 RABGEF1 Homo sapiens (Human) 220; 117; 154; 184; 426; 99; 161; 317; 165 "220,PPERVEKIMDQIE; 117,KVTTVKKFFSASS; 154,ETDRVSKEFIEFL; 184,LEGMHYKRDLSIE; 426,GVKQMYKNLDLLS; 99,QSLTFSKFEEKKT; 161,EFIEFLKTFHKTG; 317,HIFNAIKITKNEP; 165,FLKTFHKTGQEIY" 220:21890473; 117:21890473; 154:21890473; 184:21890473; 426:21890473; 99:21890473; 161:21890473; 317:21890473; 165:21890473 RABGEF1 Homo sapiens (Human) 343; 187; 191; 143; 246; 125; 452; 180; 210 "343,HIFNAIKITKNEP; 187,EFIEFLKTFHKTG; 191,FLKTFHKTGQEIY; 143,KVTTVKKFFSASS; 246,PPERVEKIMDQIE; 125,QSLTFSKFEEKKT; 452,GVKQMYKNLDLLS; 180,ETDRVSKEFIEFL; 210,LEGMHYKRDLSIE" 339:21906983; 263:21906983; 629:21906983; 267:21906983; 280:21906983; 299:21906983; 348:21906983; 520:21906983; 318:21906983 RABGEF1 Homo sapiens (Human) 85; 220; 103; 219; 123; 122; 328; 329 "85,QSLTFSKFEEKKT; 220,HIFNAIKITKNEP; 103,KVTTVKKFFSASS; 219,HIFNAIKITKNEP; 123,PPERVEKIMDQIE; 122,PPERVEKIMDQIE; 328,GVKQMYKNLDLLS; 329,GVKQMYKNLDLLS" "220:21890473; 85:21890473; 103:21906983,21890473; 116:21906983; 426:21906983; 99:21906983; 317:21906983; 123:21890473; 329:21890473" RABGEF1 Homo sapiens (Human) 85; 103; 170; 206; 140; 412; 147; 151; 303 "85,QSLTFSKFEEKKT; 103,KVTTVKKFFSASS; 170,LEGMHYKRDLSIE; 206,PPERVEKIMDQIE; 140,ETDRVSKEFIEFL; 412,GVKQMYKNLDLLS; 147,EFIEFLKTFHKTG; 151,FLKTFHKTGQEIY; 303,HIFNAIKITKNEP" 85:21890473; 103:21890473; 170:21890473; 206:21890473; 140:21890473; 412:21890473; 147:21890473; 151:21890473; 303:21890473 RABGEF1 Homo sapiens (Human) 263; 281 "263,QSLTFSKFEEKKT; 281,KVTTVKKFFSASS" "263:21139048,22053931; 281:21139048"