PTM - CPLM Gene Name UniProt ID Type Position Code USP25 Q9UHP3 Sumoylation 99 K USP25 Q9UHP3 Sumoylation 141 K USP25 Q9UHP3 Ubiquitination 15 K USP25 Q9UHP3 Ubiquitination 57 K USP25 Q9UHP3 Ubiquitination 201 K USP25 Q9UHP3 Ubiquitination 250 K USP25 Q9UHP3 Ubiquitination 254 K USP25 Q9UHP3 Ubiquitination 313 K USP25 Q9UHP3 Ubiquitination 392 K USP25 Q9UHP3 Ubiquitination 444 K USP25 Q9UHP3 Ubiquitination 520 K USP25 Q9UHP3 Ubiquitination 584 K USP25 Q9UHP3 Ubiquitination 630 K USP25 Q9UHP3 Ubiquitination 698 K USP25 Q9UHP3 Ubiquitination 710 K USP25 Q9UHP3 Ubiquitination 715 K USP25 Q9UHP3 Ubiquitination 721 K USP25 Q9UHP3 Ubiquitination 60 K USP25 Q9UHP3 Ubiquitination 99 K USP25 Q9UHP3 Ubiquitination 312 K USP25 Q9UHP3 Ubiquitination 669 K

PTM - dbPAF Gene Name UniProt ID Position Type Species USP25 Q9UHP3 147 T Homo sapiens(Human) USP25 Q9UHP3 149 T Homo sapiens(Human) USP25 Q9UHP3 200 Y Homo sapiens(Human) USP25 Q9UHP3 204 S Homo sapiens(Human) USP25 Q9UHP3 404 Y Homo sapiens(Human) USP25 Q9UHP3 680 T Homo sapiens(Human) USP25 Q9UHP3 69 Y Homo sapiens(Human) USP25 Q9UHP3 70 Y Homo sapiens(Human) USP25 Q9UHP3 727 T Homo sapiens(Human) USP25 Q9UHP3 740 Y Homo sapiens(Human) USP25 Q9UHP3 745 S Homo sapiens(Human) USP25 Q9UHP3 835 S Homo sapiens(Human) USP25 Q9UHP3 85 S Homo sapiens(Human) USP25 Q9UHP3 912 S Homo sapiens(Human) USP25 Q9UHP3 916 Y Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9UHP3 T2-p Phosphorylation ______MtVEQNVLQ Q9UHP3 S11-p Phosphorylation EQNVLQQsAAQkHQQ Q9UHP3 Y69-p Phosphorylation PQQEETTyyQTALPG Q9UHP3 Y70-p Phosphorylation QQEETTyyQTALPGN Q9UHP3 S85-p Phosphorylation DRYISVGsQADTNVI Q9UHP3 S113-p Phosphorylation IALSLAEsNRAFRET Q9UHP3 T147-p Phosphorylation NkACLKRtPtEVWRD Q9UHP3 T149-p Phosphorylation ACLKRtPtEVWRDSR Q9UHP3 Y404-p Phosphorylation QVLYLDRyMHRNREI Q9UHP3 Y425-p Phosphorylation EIKRLKDyLtVLQQR Q9UHP3 T427-p Phosphorylation KRLKDyLtVLQQRLE Q9UHP3 T680-p Phosphorylation QPLVGIEtLPPDLRD Q9UHP3 T727-p Phosphorylation QkLRESEtSVTTAQA Q9UHP3 Y740-p Phosphorylation QAAGDPEyLEQPsRS Q9UHP3 S745-p Phosphorylation PEyLEQPsRSDFSKH Q9UHP3 S912-p Phosphorylation QNNKELLsKGLyRGH Q9UHP3 Y916-p Phosphorylation ELLsKGLyRGHDEEL Q9UHP3 K99-sm Sumoylation IDLTGDDkDDLQRAI Q9UHP3 K141-sm Sumoylation EASIAENkACLKRtP Q9UHP3 K15-ub Ubiquitination LQQsAAQkHQQTFLN Q9UHP3 K57-ub Ubiquitination AVAFLTAkNAkTPQQ Q9UHP3 K60-ub Ubiquitination FLTAkNAkTPQQEET Q9UHP3 K99-ub Ubiquitination IDLTGDDkDDLQRAI Q9UHP3 K201-ub Ubiquitination RRLVLNYkPPSNAQD Q9UHP3 K250-ub Ubiquitination SRAVEILkDAFkSND Q9UHP3 K254-ub Ubiquitination EILkDAFkSNDSQQQ Q9UHP3 K312-ub Ubiquitination AVGVLEGkkFENTEM Q9UHP3 K313-ub Ubiquitination VGVLEGkkFENTEMF Q9UHP3 K392-ub Ubiquitination RPEKIHNkLEFPQVL Q9UHP3 K444-ub Ubiquitination LSYGSGPkRFPLVDV Q9UHP3 K520-ub Ubiquitination SSRSVIHkPFTQSRI Q9UHP3 K584-ub Ubiquitination IELMYSDkSMIQVPY Q9UHP3 K622-ub Ubiquitination HRESRWMkYNDIAVT Q9UHP3 K630-ub Ubiquitination YNDIAVTkSSWEELV Q9UHP3 K669-ub Ubiquitination LIQEEFNkETGQPLV Q9UHP3 K698-ub Ubiquitination EDNQRFEkELEEWDA Q9UHP3 K710-ub Ubiquitination WDAQLAQkALQEkLL Q9UHP3 K715-ub Ubiquitination AQkALQEkLLASQkL Q9UHP3 K721-ub Ubiquitination EkLLASQkLRESEtS

PTM - dbPTM UniProt ID Position Code Type Resources References Q9UHP3 99 K Sumoylation Swiss-Prot 1010711 18538659;19440361 Q9UHP3 916 Y Phosphorylation Swiss-Prot 1010711 17924679 Q9UHP3 99 K Ubiquitylation Swiss-Prot 1010711 - Q9UHP3 141 K Sumoylation Phosphositeplus.1010730 18538659 Q9UHP3 99 K Sumoylation Phosphositeplus.1010730 18538659 Q9UHP3 15 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UHP3 201 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UHP3 250 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UHP3 254 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UHP3 312 K Ubiquitylation Phosphositeplus.1010730 20972266;21906983 Q9UHP3 313 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UHP3 392 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UHP3 444 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UHP3 520 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UHP3 57 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHP3 584 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UHP3 60 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHP3 630 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UHP3 669 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHP3 698 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UHP3 710 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHP3 715 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q9UHP3 721 K Ubiquitylation Phosphositeplus.1010730 21890473 Q9UHP3 99 K Ubiquitylation Phosphositeplus.1010730 19440361;21906983 Q9UHP3 149 T Phosphorylation Phosphositeplus.1010730 20736484 Q9UHP3 69 Y Phosphorylation Phosphositeplus.1010730 20562096 Q9UHP3 740 Y Phosphorylation Phosphositeplus.1010730 20562096 Q9UHP3 916 Y Phosphorylation HPRD 9.0 17924679

PTM - HPRD Symbol Position Code Type References USP25 916 Y Phosphorylation 17924679

PTM - UniProt UniProt ID Position Type PMIDs Q9UHP3 85 Phosphoserine 23186163 Q9UHP3 740 Phosphotyrosine 19909739

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 29761 118895 - K Ubiquitination 22118674 29761 118895 - K Ubiquitination 20972266 29761 118895 - K Ubiquitination 21963094 29761 118895 - K Ubiquitination 21139048 29761 118895 - K Ubiquitination 21890473 29761 118895 - K Ubiquitination 21906983 29761 118895 - K Ubiquitination 22053931 29761 118895 - K Ubiquitination 23000965 29761 118895 - K Ubiquitination 25015289 29761 118895 - K Sumoylation 18538659 29761 118895 - K Ubiquitination 19440361

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID USP25 Homo sapiens (Human) 254; 715; 392; 698; 250; 444; 201; 630; 584; 313; 721; 57; 520; 15; 710 "254,ILKDAFKSNDSQQ; 715,QKALQEKLLASQK; 392,PEKIHNKLEFPQV; 698,DNQRFEKELEEWD; 250,RAVEILKDAFKSN; 444,SYGSGPKRFPLVD; 201,RLVLNYKPPSNAQ; 630,NDIAVTKSSWEEL; 584,ELMYSDKSMIQVP; 313,GVLEGKKFENTEM; 721,KLLASQKLRESET; 57,VAFLTAKNAKTPQ; 520,SRSVIHKPFTQSR; 15,QQSAAQKHQQTFL; 710,DAQLAQKALQEKL" 254:21890473; 715:21890473; 392:21890473; 698:21890473; 250:21890473; 444:21890473; 201:21890473; 630:21890473; 584:21890473; 313:21890473; 721:21890473; 57:21890473; 520:21890473; 15:21890473; 710:21890473 USP25 Homo sapiens (Human) 254; 715; 392; 698; 250; 444; 201; 630; 584; 313; 721; 57; 520; 15; 710 "254,ILKDAFKSNDSQQ; 715,QKALQEKLLASQK; 392,PEKIHNKLEFPQV; 698,DNQRFEKELEEWD; 250,RAVEILKDAFKSN; 444,SYGSGPKRFPLVD; 201,RLVLNYKPPSNAQ; 630,NDIAVTKSSWEEL; 584,ELMYSDKSMIQVP; 313,GVLEGKKFENTEM; 721,KLLASQKLRESET; 57,VAFLTAKNAKTPQ; 520,SRSVIHKPFTQSR; 15,QQSAAQKHQQTFL; 710,DAQLAQKALQEKL" 254:21890473; 715:21890473; 392:21890473; 698:21890473; 250:21890473; 444:21890473; 201:21890473; 630:21890473; 584:21890473; 313:21890473; 721:21890473; 57:21890473; 520:21890473; 15:21890473; 710:21890473 USP25 Homo sapiens (Human) 49; 113; 159 "49,SRSVIHKPFTQSR; 113,ELMYSDKSMIQVP; 159,NDIAVTKSSWEEL" 49:21890473; 113:21890473; 159:21890473 USP25 Homo sapiens (Human) 254; 715; 392; 698; 250; 444; 201; 630; 584; 313; 721; 57; 520; 15; 710 "254,ILKDAFKSNDSQQ; 715,QKALQEKLLASQK; 392,PEKIHNKLEFPQV; 698,DNQRFEKELEEWD; 250,RAVEILKDAFKSN; 444,SYGSGPKRFPLVD; 201,RLVLNYKPPSNAQ; 630,NDIAVTKSSWEEL; 584,ELMYSDKSMIQVP; 313,GVLEGKKFENTEM; 721,KLLASQKLRESET; 57,VAFLTAKNAKTPQ; 520,SRSVIHKPFTQSR; 15,QQSAAQKHQQTFL; 710,DAQLAQKALQEKL" 254:21890473; 715:21890473; 392:21890473; 698:21890473; 250:21890473; 444:21890473; 201:21890473; 630:21890473; 584:21890473; 313:21890473; 721:21890473; 57:21890473; 520:21890473; 15:21890473; 710:21890473 USP25 Homo sapiens (Human) 254; 715; 392; 698; 250; 444; 201; 630; 584; 313; 721; 57; 520; 15; 710 "254,ILKDAFKSNDSQQ; 715,QKALQEKLLASQK; 392,PEKIHNKLEFPQV; 698,DNQRFEKELEEWD; 250,RAVEILKDAFKSN; 444,SYGSGPKRFPLVD; 201,RLVLNYKPPSNAQ; 630,NDIAVTKSSWEEL; 584,ELMYSDKSMIQVP; 313,GVLEGKKFENTEM; 721,KLLASQKLRESET; 57,VAFLTAKNAKTPQ; 520,SRSVIHKPFTQSR; 15,QQSAAQKHQQTFL; 710,DAQLAQKALQEKL" 254:21890473; 715:21890473; 392:21890473; 698:21890473; 250:21890473; 444:21890473; 201:21890473; 630:21890473; 584:21890473; 313:21890473; 721:21890473; 57:21890473; 520:21890473; 15:21890473; 710:21890473 USP25 Homo sapiens (Human) 715; 201; 444; 99; 313; 584; 312; 520; 710; 254; 669; 392; 250; 698; 630; 721; 57; 60 "715,QKALQEKLLASQK; 201,RLVLNYKPPSNAQ; 444,SYGSGPKRFPLVD; 99,DLTGDDKDDLQRA; 313,GVLEGKKFENTEM; 584,ELMYSDKSMIQVP; 312,VGVLEGKKFENTE; 520,SRSVIHKPFTQSR; 710,DAQLAQKALQEKL; 254,ILKDAFKSNDSQQ; 669,IQEEFNKETGQPL; 392,PEKIHNKLEFPQV; 250,RAVEILKDAFKSN; 698,DNQRFEKELEEWD; 630,NDIAVTKSSWEEL; 721,KLLASQKLRESET; 57,VAFLTAKNAKTPQ; 60,LTAKNAKTPQQEE" "715:21890473,21906983; 201:21890473,21906983,22053931; 444:21890473,22053931; 99:21906983; 313:21890473; 584:21890473,21906983,22053931; 312:21906983; 520:21890473; 710:21890473,21906983; 254:21890473,21906983; 669:21906983,22053931; 392:21890473; 250:21890473; 698:21890473; 630:21890473,21906983,22053931; 721:21890473; 57:21890473,21906983; 60:21906983" USP25 Homo sapiens (Human) 715; 201; 444; 99; 313; 584; 312; 520; 710; 254; 669; 392; 250; 698; 630; 721; 57; 60 "715,QKALQEKLLASQK; 201,RLVLNYKPPSNAQ; 444,SYGSGPKRFPLVD; 99,DLTGDDKDDLQRA; 313,GVLEGKKFENTEM; 584,ELMYSDKSMIQVP; 312,VGVLEGKKFENTE; 520,SRSVIHKPFTQSR; 710,DAQLAQKALQEKL; 254,ILKDAFKSNDSQQ; 669,IQEEFNKETGQPL; 392,PEKIHNKLEFPQV; 250,RAVEILKDAFKSN; 698,DNQRFEKELEEWD; 630,NDIAVTKSSWEEL; 721,KLLASQKLRESET; 57,VAFLTAKNAKTPQ; 60,LTAKNAKTPQQEE" "715:21890473,21906983; 201:21890473,21906983; 444:21890473; 99:21906983; 313:21890473; 584:21890473,21906983; 312:21906983; 520:21890473; 710:21890473,21906983; 254:21890473,21906983; 669:21906983; 392:21890473; 250:21890473; 698:21890473; 630:21890473,21906983; 721:21890473; 57:21890473,21906983; 60:21906983" USP25 Homo sapiens (Human) 49; 113; 159 "49,SRSVIHKPFTQSR; 113,ELMYSDKSMIQVP; 159,NDIAVTKSSWEEL" 98:21906983; 52:21906983 USP25 Homo sapiens (Human) 254; 201; 250; 57; 15 "254,ILKDAFKSNDSQQ; 201,RLVLNYKPPSNAQ; 250,RAVEILKDAFKSN; 57,VAFLTAKNAKTPQ; 15,QQSAAQKHQQTFL" 254:21890473; 201:21890473; 250:21890473; 57:21890473; 15:21890473