PTM - CPLM Gene Name UniProt ID Type Position Code NCK1; NCK P16333 Ubiquitination 286 K

PTM - dbPAF Gene Name UniProt ID Position Type Species NCK1; NCK P16333 105 Y Homo sapiens(Human) NCK1; NCK P16333 112 Y Homo sapiens(Human) NCK1; NCK P16333 13 Y Homo sapiens(Human) NCK1; NCK P16333 147 S Homo sapiens(Human) NCK1; NCK P16333 148 Y Homo sapiens(Human) NCK1; NCK P16333 161 T Homo sapiens(Human) NCK1; NCK P16333 166 S Homo sapiens(Human) NCK1; NCK P16333 246 Y Homo sapiens(Human) NCK1; NCK P16333 262 S Homo sapiens(Human) NCK1; NCK P16333 268 Y Homo sapiens(Human) NCK1; NCK P16333 272 S Homo sapiens(Human) NCK1; NCK P16333 312 S Homo sapiens(Human) NCK1; NCK P16333 313 S Homo sapiens(Human) NCK1; NCK P16333 339 Y Homo sapiens(Human) NCK1; NCK P16333 347 S Homo sapiens(Human) NCK1; NCK P16333 53 S Homo sapiens(Human) NCK1; NCK P16333 61 S Homo sapiens(Human) NCK1; NCK P16333 66 S Homo sapiens(Human) NCK1; NCK P16333 74 T Homo sapiens(Human) NCK1; NCK P16333 85 S Homo sapiens(Human) NCK1; NCK P16333 89 S Homo sapiens(Human) NCK1; NCK P16333 91 S Homo sapiens(Human) NCK1; NCK P16333 96 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P16333 Y13-p Phosphorylation VVVAKFDyVAQQEQE P16333 S61-p Phosphorylation NYVERKNsARKAsIV P16333 S66-p Phosphorylation KNsARKAsIVKNLKD P16333 T74-p Phosphorylation IVKNLKDtLGIGKVK P16333 S85-p Phosphorylation GKVKRKPsVPdsAsP P16333 S89-p Phosphorylation RKPsVPdsAsPADDs P16333 S91-p Phosphorylation PsVPdsAsPADDsFV P16333 S96-p Phosphorylation sAsPADDsFVDPGER P16333 Y105-p Phosphorylation VDPGERLyDLNMPAy P16333 Y112-p Phosphorylation yDLNMPAyVKFNYMA P16333 S166-p Phosphorylation YVTEEGDsPLGDHVG P16333 S262-p Phosphorylation LTSGLEPsPPQCDyI P16333 Y268-p Phosphorylation PsPPQCDyIRPSLTG P16333 S313-p Phosphorylation LIRDSESsPNDFSVS P16333 Y339-p Phosphorylation VQLKETVyCIGQRKF P16333 S347-p Phosphorylation CIGQRKFsTMEELVE P16333 K178-ub Ubiquitination HVGSLSEkLAAVVNN

PTM - dbPTM UniProt ID Position Code Type Resources References P16333 2 A Acetylation Swiss-Prot 1010711 19413330 P16333 85 S Phosphorylation Swiss-Prot 1010711 17287340;18669648;19690332 P16333 91 S Phosphorylation Swiss-Prot 1010711 18669648 P16333 96 S Phosphorylation Swiss-Prot 1010711 17287340;18669648;19690332 P16333 105 Y Phosphorylation Swiss-Prot 1010711 15951569;17287340;17389395;18088087;19534553;19690332 P16333 85 S Phosphorylation PhosphoELM.10011 18669648;17287340;17081983 P16333 89 S Phosphorylation PhosphoELM.10011 18669648 P16333 91 S Phosphorylation PhosphoELM.10011 18669648 P16333 96 S Phosphorylation PhosphoELM.10011 18669648;17287340 P16333 166 S Phosphorylation PhosphoELM.10011 18669648 P16333 161 T Phosphorylation PhosphoELM.10011 18669648 P16333 105 Y Phosphorylation PhosphoELM.10011 17287340;18088087 P16333 268 Y Phosphorylation PhosphoELM.10011 16497976 P16333 88 D Caspase Phosphositeplus.1010730 18722006;18724940 P16333 166 S Phosphorylation Phosphositeplus.1010730 19690332 P16333 85 S Phosphorylation Phosphositeplus.1010730 17287340;18669648;19276368;19690332;20007894;20044836;20164059;20166139;20363803;20562096;20815410;21253578 P16333 89 S Phosphorylation Phosphositeplus.1010730 20166139;20815410;21253578 P16333 91 S Phosphorylation Phosphositeplus.1010730 18669648;20166139;20815410 P16333 96 S Phosphorylation Phosphositeplus.1010730 17287340;18669648;19690332;20068231 P16333 105 Y Phosphorylation Phosphositeplus.1010730 15951569;17016520;17287340;17360941;17389395;18083107;18088087;18180459;18707149;19534553;19605366;19690332;20007894;20068231;20090780;20562096;20736484;21253578;21394647 P16333 112 Y Phosphorylation Phosphositeplus.1010730 18083107;18180459;20007894;20736484;21403953;21447384 P16333 268 Y Phosphorylation Phosphositeplus.1010730 16497976 P16333 85 S Phosphorylation HPRD 9.0 15144186,18669648,17287340,19651622,20166139,20068231 P16333 89 S Phosphorylation HPRD 9.0 18669648,19651622,20166139 P16333 91 S Phosphorylation HPRD 9.0 17081983,18669648,20166139,20068231 P16333 96 S Phosphorylation HPRD 9.0 18669648,17287340,20068231 P16333 166 S Phosphorylation HPRD 9.0 18669648,20068231 P16333 262 S Phosphorylation HPRD 9.0 20068231 P16333 312 S Phosphorylation HPRD 9.0 20068231 P16333 313 S Phosphorylation HPRD 9.0 20068231 P16333 74 T Phosphorylation HPRD 9.0 18212344 P16333 161 T Phosphorylation HPRD 9.0 18669648 P16333 105 Y Phosphorylation HPRD 9.0 15951569,17287340,17389395,18088087,17016520,18707149,20068231 P16333 246 Y Phosphorylation HPRD 9.0 16497976 P16333 268 Y Phosphorylation HPRD 9.0 16497976 P16333 85 S Phosphorylation SysPTM 1.1 18669648;17081983 P16333 91 S Phosphorylation SysPTM 1.1 18669648;17081983 P16333 96 S Phosphorylation SysPTM 1.1 18669648 P16333 74 T Phosphorylation SysPTM 1.1 18212344 P16333 105 Y Phosphorylation SysPTM 1.1 18707149;18088087

PTM - HPRD Symbol Position Code Type References NCK1 105 Y Phosphorylation 15951569,17287340,17389395,18088087,17016520,18707149,20068231 NCK1 161 T Phosphorylation 18669648 NCK1 166 S Phosphorylation 18669648,20068231 NCK1 246 Y Phosphorylation 16497976 NCK1 262 S Phosphorylation 20068231 NCK1 268 Y Phosphorylation 16497976 NCK1 312 S Phosphorylation 20068231 NCK1 313 S Phosphorylation 20068231 NCK1 74 T Phosphorylation 18212344 NCK1 85 S Phosphorylation 15144186,18669648,17287340,19651622,20166139,20068231 NCK1 89 S Phosphorylation 18669648,19651622,20166139 NCK1 91 S Phosphorylation 17081983,18669648,20166139,20068231 NCK1 96 S Phosphorylation 18669648,17287340,20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P16333 161 T 18669648 HTP Phosphorylation P16333 96 S 18669648 HTP Phosphorylation P16333 96 S 17287340 HTP Phosphorylation P16333 166 S 18669648 HTP Phosphorylation P16333 105 Y 17287340 HTP Phosphorylation P16333 105 Y 18088087 HTP Phosphorylation P16333 268 Y 16497976 HTP Phosphorylation P16333 85 S 18669648 HTP Phosphorylation P16333 85 S 17287340 HTP Phosphorylation P16333 85 S 17081983 HTP Phosphorylation P16333 89 S 18669648 HTP Phosphorylation P16333 91 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P16333 2 N-acetylalanine 22223895;22814378 P16333 85 Phosphoserine 18669648;19690332;23186163;24275569 P16333 89 Phosphoserine 24275569 P16333 91 Phosphoserine 18669648;23186163 P16333 96 Phosphoserine 18669648;23186163 P16333 105 Phosphotyrosine 18088087;19690332;20068231 P16333 166 Phosphoserine 23186163;24275569

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type NCK1 IPI00028065 S 166 VTEEGDpSPLGDHV phosphorylation NCK1 IPI00028065 S 262 TSGLEPpSPPQCDY phosphorylation NCK1 IPI00028065 S 312 LIRDSEpSSPNDFS phosphorylation NCK1 IPI00028065 S 85 KVKRKPpSVPDSAS phosphorylation NCK1 IPI00028065 S 91 SVPDSApSPADDSF phosphorylation NCK1 IPI00028065 S 96 ASPADDpSFVDPGE phosphorylation NCK1 IPI00028065 Y 105 DPGERLpYDLNMPA phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 4690 110770 - K Ubiquitination 24287595 4690 110770 - K Ubiquitination 24816145