PTM - CPLM Gene Name UniProt ID Type Position Code NSD1; ARA267; KMT3B Q96L73 Acetylation 2086 K NSD1; ARA267; KMT3B Q96L73 Acetylation 2095 K NSD1; ARA267; KMT3B Q96L73 Ubiquitination 865 K NSD1; ARA267; KMT3B Q96L73 Ubiquitination 886 K

PTM - dbPAF Gene Name UniProt ID Position Type Species NSD1; ARA267; KMT3B Q96L73 1024 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1027 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1028 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1042 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1063 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1077 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1143 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1208 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1240 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1243 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1257 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1269 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1334 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1338 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1382 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1446 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1454 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1507 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1509 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1510 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1694 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1815 Y Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1886 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1890 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1987 Y Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 1994 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 209 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2109 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 214 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 217 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 224 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 233 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2330 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2334 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2341 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2364 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2369 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2374 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2378 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2379 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2382 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2395 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2397 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2462 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2471 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2476 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2560 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2571 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2573 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2599 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 2623 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 316 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 317 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 343 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 451 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 483 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 486 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 563 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 572 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 589 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 726 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 752 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 758 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 760 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 766 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 780 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 822 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 849 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 850 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 854 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 935 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 936 Y Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 938 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 948 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 958 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 961 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 962 T Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 965 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 975 S Homo sapiens(Human) NSD1; ARA267; KMT3B Q96L73 979 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q96L73 K606-ac Acetylation ISKCSREkNKPQRSL Q96L73 K1299-ac Acetylation FAPKKKQkkVQEQVH Q96L73 K1300-ac Acetylation APKKKQkkVQEQVHK Q96L73 K1480-ac Acetylation RQRHAAAkMQCKKVK Q96L73 K2086-ac Acetylation GFLGVRPkNQPIATE Q96L73 K2095-ac Acetylation QPIATEEkSKKFKKK Q96L73 K501-m1 Methylation CAKSRARkSSDNPKR Q96L73 R1986-m2 Methylation EECRARIryAQEHDI Q96L73 R2385-m1 Methylation TsVPtGLrLPPPDRL Q96L73 K2506-m1 Methylation HMPRAVEkGCVSDPL Q96L73 K2518-m1 Methylation DPLQTSGkAAAPSED Q96L73 K2581-m1 Methylation PGLVKQAkQMVGGQQ Q96L73 S117-p Phosphorylation PIVCTSLsPGGPTAL Q96L73 T214-p Phosphorylation MGSEQDStPESRHGA Q96L73 S224-p Phosphorylation SRHGAVKsPFLPLAP Q96L73 T233-p Phosphorylation FLPLAPQtETQKNKQ Q96L73 T317-p Phosphorylation CQPKKKStPLkYEVG Q96L73 S343-p Phosphorylation WWPCRICsDPLINTH Q96L73 S451-p Phosphorylation PGSIKLDsEEDMPFE Q96L73 S483-p Phosphorylation LKSLAFDsEHsADEK Q96L73 S486-p Phosphorylation LAFDsEHsADEKEKP Q96L73 T563-p Phosphorylation NSLTGSNtAPGSFLF Q96L73 S589-p Phosphorylation FETSNGDsLLGLPEG Q96L73 S726-p Phosphorylation AEPGTETsQVNLSDL Q96L73 S752-p Phosphorylation DFTNDALsPKFNLSS Q96L73 S780-p Phosphorylation ANQALLHsKSKQPKF Q96L73 S811-p Phosphorylation PVINEECsLKCCSSD Q96L73 S822-p Phosphorylation CSSDTKGsPLASISK Q96L73 S849-p Phosphorylation MHEKTRDssDIEtAV Q96L73 S850-p Phosphorylation HEKTRDssDIEtAVV Q96L73 T854-p Phosphorylation RDssDIEtAVVKHVL Q96L73 Y936-p Phosphorylation TAQNLVSyRsPGRGD Q96L73 S938-p Phosphorylation QNLVSyRsPGRGDCS Q96L73 S948-p Phosphorylation RGDCSTNsPVGVSKV Q96L73 S958-p Phosphorylation GVSKVLVsGGsTHNs Q96L73 S961-p Phosphorylation KVLVsGGsTHNsEKK Q96L73 S965-p Phosphorylation sGGsTHNsEKKGDGT Q96L73 S1024-p Phosphorylation TRRNCGRsKPSSKLR Q96L73 T1042-p Phosphorylation SAQMVKNtVNRKALK Q96L73 S1077-p Phosphorylation GDRERGGsLRGGAED Q96L73 S1143-p Phosphorylation ELDSVMNsENDELNG Q96L73 T1208-p Phosphorylation DEFPEHRtPsASILE Q96L73 S1210-p Phosphorylation FPEHRtPsASILEEP Q96L73 S1243-p Phosphorylation VCDKSSAsIGDMEKE Q96L73 T1257-p Phosphorylation EPGIPSLtPQAELPE Q96L73 S1269-p Phosphorylation LPEPAVRsEKKRLRK Q96L73 S1334-p Phosphorylation NKQVDENsLIStKEE Q96L73 T1338-p Phosphorylation DENsLIStKEEPPVL Q96L73 S1382-p Phosphorylation VPVAPEVsPRPALES Q96L73 T1446-p Phosphorylation GHLENGItESCATSY Q96L73 S1454-p Phosphorylation ESCATSYsKDFGGGT Q96L73 T1507-p Phosphorylation GELMPHRtATsPKET Q96L73 S1510-p Phosphorylation MPHRtATsPKETVEE Q96L73 T1694-p Phosphorylation IICPNHFtPRRGCRN Q96L73 Y1815-p Phosphorylation HQARVFPyMEGDVSS Q96L73 T1886-p Phosphorylation IGRVQIFtADLsEIP Q96L73 S1890-p Phosphorylation QIFtADLsEIPRCNC Q96L73 Y1987-p Phosphorylation ECRARIryAQEHDIt Q96L73 T1994-p Phosphorylation yAQEHDItNFYMLTL Q96L73 S2288-p Phosphorylation RPLERTDsRPQPLDK Q96L73 S2334-p Phosphorylation PQLSDKPsPVTSPSS Q96L73 S2364-p Phosphorylation ADPRLDKsIGAAsPR Q96L73 S2369-p Phosphorylation DKsIGAAsPRPQsLE Q96L73 S2374-p Phosphorylation AAsPRPQsLEKTsVP Q96L73 S2379-p Phosphorylation PQsLEKTsVPtGLrL Q96L73 T2382-p Phosphorylation LEKTsVPtGLrLPPP Q96L73 T2462-p Phosphorylation VMDLIDLtPRQKERA Q96L73 S2471-p Phosphorylation RQKERAAsPHQVtPQ Q96L73 T2476-p Phosphorylation AAsPHQVtPQADEKM Q96L73 S2560-p Phosphorylation TQASGRAsAGAEQTP Q96L73 S2573-p Phosphorylation TPGPLSQsPGLVKQA Q96L73 S2623-p Phosphorylation kLVTTEQsPWALGKA Q96L73 K320-sm Sumoylation KKKStPLkYEVGDLI Q96L73 K837-sm Sumoylation SGKVDGLkLLNNMHE Q96L73 K1411-sm Sumoylation RQRKPTKkLLESNDL Q96L73 K2545-sm Sumoylation LLSQPPAkAFLYEPT Q96L73 K2616-sm Sumoylation SLPTEEKkLVTTEQs Q96L73 K865-ub Ubiquitination KHVLSELkELSYRSL Q96L73 K886-ub Ubiquitination SGTSKPSkPLLFSSA

PTM - dbPTM UniProt ID Position Code Type Resources References Q96L73 209 S Phosphorylation Swiss-Prot 1010711 19369195 Q96L73 483 S Phosphorylation Swiss-Prot 1010711 19413330 Q96L73 486 S Phosphorylation Swiss-Prot 1010711 19413330 Q96L73 766 S Phosphorylation Swiss-Prot 1010711 17487921 Q96L73 2471 S Phosphorylation Swiss-Prot 1010711 18669648 Q96L73 316 S Phosphorylation PhosphoELM.10011 18669648 Q96L73 2471 S Phosphorylation PhosphoELM.10011 18669648 Q96L73 865 K Ubiquitylation Phosphositeplus.1010730 21890473 Q96L73 886 K Ubiquitylation Phosphositeplus.1010730 21890473 Q96L73 2086 K Acetylation Phosphositeplus.1010730 20167786 Q96L73 2095 K Acetylation Phosphositeplus.1010730 20167786 Q96L73 1024 S Phosphorylation Phosphositeplus.1010730 20068231 Q96L73 2369 S Phosphorylation Phosphositeplus.1010730 19276368;19664995 Q96L73 2374 S Phosphorylation Phosphositeplus.1010730 19276368 Q96L73 2471 S Phosphorylation Phosphositeplus.1010730 18669648;19664995;20363803;21406692 Q96L73 2573 S Phosphorylation Phosphositeplus.1010730 19664995;20068231 Q96L73 483 S Phosphorylation Phosphositeplus.1010730 20068231;20363803;21406692;22115753 Q96L73 486 S Phosphorylation Phosphositeplus.1010730 20068231;20363803;21406692;22115753 Q96L73 780 S Phosphorylation Phosphositeplus.1010730 20068231 Q96L73 822 S Phosphorylation Phosphositeplus.1010730 21406692 Q96L73 938 S Phosphorylation Phosphositeplus.1010730 19276368 Q96L73 961 S Phosphorylation Phosphositeplus.1010730 20068231 Q96L73 1338 T Phosphorylation Phosphositeplus.1010730 20068231 Q96L73 2462 T Phosphorylation Phosphositeplus.1010730 20068231 Q96L73 1815 Y Phosphorylation Phosphositeplus.1010730 20736484 Q96L73 936 Y Phosphorylation Phosphositeplus.1010730 21403953 Q96L73 217 S Phosphorylation HPRD 9.0 19413330,19651622,20068231 Q96L73 316 S Phosphorylation HPRD 9.0 18669648 Q96L73 483 S Phosphorylation HPRD 9.0 19413330,19651622,20068231 Q96L73 486 S Phosphorylation HPRD 9.0 19413330,19651622,20068231 Q96L73 758 S Phosphorylation HPRD 9.0 20068231 Q96L73 780 S Phosphorylation HPRD 9.0 20068231 Q96L73 961 S Phosphorylation HPRD 9.0 20068231 Q96L73 1024 S Phosphorylation HPRD 9.0 20068231 Q96L73 1027 S Phosphorylation HPRD 9.0 20068231 Q96L73 2330 S Phosphorylation HPRD 9.0 17081983 Q96L73 2369 S Phosphorylation HPRD 9.0 19664995,20068231 Q96L73 2471 S Phosphorylation HPRD 9.0 18669648,19664995,19651622 Q96L73 2573 S Phosphorylation HPRD 9.0 19664995,20068231 Q96L73 2599 S Phosphorylation HPRD 9.0 17081983 Q96L73 962 T Phosphorylation HPRD 9.0 20068231 Q96L73 1338 T Phosphorylation HPRD 9.0 20068231 Q96L73 2109 T Phosphorylation HPRD 9.0 20068231 Q96L73 2378 T Phosphorylation HPRD 9.0 20068231 Q96L73 2462 T Phosphorylation HPRD 9.0 20068231 Q96L73 2471 S Phosphorylation SysPTM 1.1 18669648 Q96L73 2599 S Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References NSD1 1024 S Phosphorylation 20068231 NSD1 1027 S Phosphorylation 20068231 NSD1 1069 T Phosphorylation 20068231 NSD1 1338 T Phosphorylation 20068231 NSD1 2100 S Phosphorylation 19664995,20068231 NSD1 2109 T Phosphorylation 20068231 NSD1 214 S Phosphorylation 19413330,19651622,20068231 NSD1 217 S Phosphorylation 19413330,19651622,20068231 NSD1 2193 T Phosphorylation 20068231 NSD1 2202 S Phosphorylation 18669648,19664995,19651622 NSD1 2304 S Phosphorylation 19664995,20068231 NSD1 2330 S Phosphorylation 17081983 NSD1 2369 S Phosphorylation 19664995,20068231 NSD1 2378 T Phosphorylation 20068231 NSD1 2462 T Phosphorylation 20068231 NSD1 2471 S Phosphorylation 18669648,19664995,19651622 NSD1 2573 S Phosphorylation 19664995,20068231 NSD1 2599 S Phosphorylation 17081983 NSD1 316 S Phosphorylation 18669648 NSD1 47 S Phosphorylation 18669648 NSD1 483 S Phosphorylation 19413330,19651622,20068231 NSD1 486 S Phosphorylation 19413330,19651622,20068231 NSD1 511 S Phosphorylation 20068231 NSD1 692 S Phosphorylation 20068231 NSD1 693 T Phosphorylation 20068231 NSD1 755 S Phosphorylation 20068231 NSD1 758 S Phosphorylation 20068231 NSD1 780 S Phosphorylation 20068231 NSD1 961 S Phosphorylation 20068231 NSD1 962 T Phosphorylation 20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q96L73 2471 S 18669648 HTP Phosphorylation Q96L73 316 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q96L73 483 Phosphoserine 21406692;23186163 Q96L73 486 Phosphoserine 21406692;23186163 Q96L73 766 Phosphoserine 17487921 Q96L73 2369 Phosphoserine 23186163 Q96L73 2462 Phosphothreonine 20068231 Q96L73 2471 Phosphoserine 18669648;23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type NSD1 IPI00102107 S 2369 KSIGAApSPRPQSL phosphorylation NSD1 IPI00102107 S 780 NQALLHpSKSKQPK phosphorylation NSD1 IPI00102107 S 961 VLVSGGpSTHNSEK phosphorylation NSD1 IPI00102107 S 965 GGSTHNpSEKKGDG phosphorylation NSD1 IPI00102107 T 1063 NQLPSVpTLDAVLQ phosphorylation NSD1 IPI00102107 T 1338 ENSLISpTKEEPPV phosphorylation NSD1 IPI00102107 T 962 LVSGGSpTHNSEKK phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 64324 122135 - K Ubiquitination 21139048 64324 122135 - K Ubiquitination 21890473

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID NSD1 Homo sapiens (Human) 596; 617 "596,HVLSELKELSYRS; 617,GTSKPSKPLLFSS" 596:21890473; 617:21890473 NSD1 Homo sapiens (Human) 596; 617 "596,HVLSELKELSYRS; 617,GTSKPSKPLLFSS" 596:21890473; 617:21890473 NSD1 Homo sapiens (Human) 865; 886 "865,HVLSELKELSYRS; 886,GTSKPSKPLLFSS" 865:21890473; 886:21890473 NSD1 Homo sapiens (Human) 596; 617 "596,HVLSELKELSYRS; 617,GTSKPSKPLLFSS" 596:21890473; 617:21890473 NSD1 Homo sapiens (Human) 762; 783 "762,HVLSELKELSYRS; 783,GTSKPSKPLLFSS" 762:21890473; 783:21890473 NSD1 Homo sapiens (Human) 865; 886 "865,HVLSELKELSYRS; 886,GTSKPSKPLLFSS" 865:21890473; 886:21890473 NSD1 Homo sapiens (Human) 596; 617 "596,HVLSELKELSYRS; 617,GTSKPSKPLLFSS" 596:21890473; 617:21890473 NSD1 Homo sapiens (Human) 596; 617 "596,HVLSELKELSYRS; 617,GTSKPSKPLLFSS" 596:21890473; 617:21890473 NSD1 Homo sapiens (Human) 596; 617 "596,HVLSELKELSYRS; 617,GTSKPSKPLLFSS" 596:21890473; 617:21890473 NSD1 Homo sapiens (Human) 596; 617 "596,HVLSELKELSYRS; 617,GTSKPSKPLLFSS" 596:21890473; 617:21890473 NSD1 Homo sapiens (Human) 596; 617 "596,HVLSELKELSYRS; 617,GTSKPSKPLLFSS" 596:21890473; 617:21890473