PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus SPSB4 PAWR 20561531 biogrid,intact exp G SPSB4 RNF7 15601820 biogrid,intact exp G MET SPSB4 15713673 biogrid exp G NOS2 SPSB4 20603330,21199876 biogrid exp CUL5 SPSB4 15601820 biogrid,intact exp, ortho G SPSB4 DDX4 20561531 biogrid exp, ortho G TCEB1 SPSB4 19159283,15601820,21199876, 25402006 biogrid,intact exp, ortho, pred G TCEB2 SPSB4 15601820, 25402006 biogrid,intact exp, pred G SIRT2 SPSB4 ortho G CDK2 SPSB4 ortho G RPL38 SPSB4 ortho G NUP133 SPSB4 ortho G CDK6 SPSB4 ortho G CDK20 SPSB4 ortho G SPSB4 ELOVL4 ortho G PLK4 SPSB4 ortho G KLC1 SPSB4 ortho G PAX6 SPSB4 ortho G AKT2 SPSB4 ortho G MARK3 SPSB4 ortho G ARHGAP1 SPSB4 ortho G CCNG1 SPSB4 ortho G CCNH SPSB4 ortho G FRA10AC1 SPSB4 ortho G PLD3 SPSB4 ortho G E2F5 SPSB4 ortho G FKBP2 SPSB4 ortho G CCNA2 SPSB4 ortho G NTN1 SPSB4 ortho G SPSB4 G3BP2 16082366 pred G RASGRP2 SPSB4 16082366 pred SPSB4 SPSB2 25402006, 16082366 pred G SOCS4 SPSB4 25402006 pred G SPSB4 FEM1B 25402006 pred G LRRC41 SPSB4 25402006 pred G G3BP1 SPSB4 16082366 pred G RGL4 SPSB4 16082366 pred CUL2 SPSB4 25402006 pred G SPSB4 KCNH4 21836163 pred G SPSB4 SPSB1 25402006, 16082366 pred G SPSB4 LRR1 25402006 pred G RAPGEF3 SPSB4 16082366 pred SOCS2 SPSB4 25402006 pred G RASGEF1C SPSB4 16082366 pred G RGL2 SPSB4 16082366 pred G SOS2 SPSB4 16082366 pred G RASGEF1B SPSB4 16082366 pred RAPGEF1 SPSB4 16082366 pred G GPS2 SPSB4 16082366 pred G SPSB4 RAPGEFL1 16082366 pred RASGRP1 SPSB4 16082366 pred SOCS5 SPSB4 25402006 pred G SPSB4 RAB40C 25402006 pred G SH2D3C SPSB4 16082366 pred G Fos39347_1 SPSB4 25402006 pred RALGDS SPSB4 16082366 pred G BCAR3 SPSB4 16082366 pred G SPSB4 RGL1 16082366 pred G RASGRF2 SPSB4 16082366 pred G SOS1 SPSB4 16082366 pred G RALGPS2 SPSB4 16082366 pred G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q96A44 Q15369-2 two hybrid 19159283 direct interaction X 2 Q96A44 Q15369-2 affinity chromatography technology 19159283 physical association X 2 Q96A44 B4DLF5 affinity chromatography technology 15713673 physical association X 2 Q96A44 Q93034 affinity chromatography technology 15601820 physical association X 2 Q96A44 Q15370-2 affinity chromatography technology 15601820 physical association X 2 Q96A44 Q15369-2 affinity chromatography technology 15601820 physical association X 2 Q96A44 Q15369-2 affinity chromatography technology 21199876 physical association X 2 Q96IZ0 Q96A44 unspecified method 20561531 direct interaction X 2 Q96A44 Q9NQI0-4 unspecified method 20561531 direct interaction X 2 Q96A44 Q9UBF6-2 affinity chromatography technology 15601820 physical association X 2 P0CG48 Q96A44 affinity chromatography technology 25015289 physical association X 2 P35228 Q96A44 affinity chromatography technology 21199876 physical association X 2 P35228 Q96A44 affinity chromatography technology 21199876 physical association X 2 P35228 Q96A44 pull down 20603330 direct interaction X 2 Q96IZ0 Q96A44 isothermal titration calorimetry 20561531 direct interaction X 2 A0A024R7Y5 Q96A44 anti tag coimmunoprecipitation 19159283 physical association X 2 P09052 Q96A44 isothermal titration calorimetry 20561531 direct interaction X 2 Q96A44 anti tag coimmunoprecipitation 15601820 association C 5

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B SPSB4 RNF7 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation) 15601820 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0914(association)|MI:0914(association) (biogrid)|(intact)|(intact) BIOGRID_250377|UniProt_164463|UniProt_80346 bait|bait|bait prey|prey|prey MET SPSB4 MI:0004(affinity chromatography technology) 15713673 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_200411 bait prey SPSB4 DDX4 MI:0686(unspecified method) 20561531 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_196989 bait prey TCEB1 SPSB4 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0004(affinity chromatography technology)|MI:0231(mammalian protein protein interaction trap)|MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation) 15601820,19159283,21199876 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0914(association)|MI:0914(association) (biogrid)|(biogrid)|(biogrid)|(intact)|(biogrid)|(intact)|(intact) BIOGRID_197011|BIOGRID_197021|BIOGRID_146931|UniProt_98783|BIOGRID_250379|UniProt_164463|UniProt_80346 bait|prey|bait|prey|prey|prey|prey prey|bait|prey|bait|bait|bait|bait CUL5 SPSB4 MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 15601820 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0914(association)|MI:0914(association)|MI:0915(physical association) (intact)|(intact)|(biogrid) UniProt_164463|UniProt_80346|BIOGRID_250376 prey|prey|prey bait|bait|bait TCEB2 SPSB4 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation)|MI:0007(anti tag coimmunoprecipitation) 15601820 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0914(association)|MI:0914(association) (biogrid)|(intact)|(intact) BIOGRID_250378|UniProt_164463|UniProt_80346 prey|prey|prey bait|bait|bait NOS2 SPSB4 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0096(pull down) 20603330,21199876 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction) (biogrid)|(biogrid)|(biogrid) BIOGRID_146921|BIOGRID_146919|BIOGRID_163763 prey|bait|prey bait|prey|bait SPSB4 PAWR MI:0114(x-ray crystallography)|MI:0114(x-ray crystallography)|MI:0686(unspecified method)|MI:0114(x-ray crystallography) 20561531 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction) (intact)|(intact)|(biogrid)|(intact) UniProt_43880|UniProt_106966|BIOGRID_196990|UniProt_170275 neutral component|neutral component|bait|neutral component neutral component|neutral component|prey|neutral component

PPI - HINT Gene A Gene B PMID Method Quality NOS2 SPSB4 20603330,21199876 0096,0004 LC TCEB1 SPSB4 21199876,15601820,19159283,19159283,19159283,15601820 0004,0007,0004,0007,0018,0004 LC NOS2 SPSB4 20603330,21199876 0096,0004 LC TCEB1 SPSB4 21199876,15601820,19159283,19159283,19159283,15601820 0004,0007,0004,0007,0018,0004 LC TCEB1 SPSB4 19159283,19159283,19159283 0004,0007,0018 LC SPSB4 PAWR 20561531 0065 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q96A44 P08581 SPSB4 MET 0.21 15713673 Q96A44 P09052 SPSB4 VAS 0.309 20561531 Q96A44 Q15370 SPSB4 TCEB2 0.236 15601820 Q96A44 Q93034 SPSB4 CUL5 0.236 15601820 Q96IZ0 Q96A44 PAWR SPSB4 0.523 20561531 Q9UBF6 Q96A44 RNF7 SPSB4 0.236 15601820 Q96A44 Q15369 SPSB4 TCEB1 0.74 19159283,15601820,21199876 Q9NQI0 Q96A44 DDX4 SPSB4 0.309 20561531 Q96A44 P35228 SPSB4 NOS2 0.569 21199876,20603330

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 PLK4 SPSB4 MI:0362 inference MI:0461 interaction database NR5A2 SPSB4 MI:0362 inference MI:0461 interaction database NTN3 SPSB4 MI:0362 inference MI:0461 interaction database ULK1 SPSB4 MI:0362 inference MI:0461 interaction database NTN1 SPSB4 MI:0362 inference MI:0461 interaction database MET SPSB4 MI:0045 experimental interaction detection MI:0461 interaction database FBXO45 SPSB4 MI:0362 inference MI:0461 interaction database UBC SPSB4 MI:0045 experimental interaction detection MI:0461 interaction database CDK4 SPSB4 MI:0362 inference MI:0461 interaction database CCNA2 SPSB4 MI:0362 inference MI:0461 interaction database CDK2 SPSB4 MI:0362 inference MI:0461 interaction database PAX6 SPSB4 MI:0362 inference MI:0461 interaction database PPIF SPSB4 MI:0362 inference MI:0461 interaction database AKT2 SPSB4 MI:0362 inference MI:0461 interaction database NOS2 SPSB4 MI:0045 experimental interaction detection MI:0461 interaction database NASP SPSB4 MI:0362 inference MI:0461 interaction database AFF2 SPSB4 MI:0362 inference MI:0461 interaction database CCNH SPSB4 MI:0362 inference MI:0461 interaction database CCNG1 SPSB4 MI:0362 inference MI:0461 interaction database RPS29 SPSB4 MI:0362 inference MI:0461 interaction database RPL38 SPSB4 MI:0362 inference MI:0461 interaction database SKP1 SPSB4 MI:0362 inference MI:0461 interaction database CCNA1 SPSB4 MI:0362 inference MI:0461 interaction database ARHGAP8 SPSB4 MI:0362 inference MI:0461 interaction database CDK3 SPSB4 MI:0362 inference MI:0461 interaction database CDK6 SPSB4 MI:0362 inference MI:0461 interaction database KLC1 SPSB4 MI:0362 inference MI:0461 interaction database ARHGAP1 SPSB4 MI:0362 inference MI:0461 interaction database NR5A1 SPSB4 MI:0362 inference MI:0461 interaction database TCEB1 SPSB4 MI:0045 experimental interaction detection MI:0461 interaction database TCEB2 SPSB4 MI:0045 experimental interaction detection MI:0461 interaction database FRA10AC1 SPSB4 MI:0362 inference MI:0461 interaction database PLD3 SPSB4 MI:0362 inference MI:0461 interaction database SIRT2 SPSB4 MI:0362 inference MI:0461 interaction database ULK2 SPSB4 MI:0362 inference MI:0461 interaction database CDK20 SPSB4 MI:0362 inference MI:0461 interaction database NUP133 SPSB4 MI:0362 inference MI:0461 interaction database CUL5 SPSB4 MI:0045 experimental interaction detection MI:0461 interaction database SPSB4 EAF2 MI:0362 inference MI:0461 interaction database SPSB4 PAWR MI:0045 experimental interaction detection MI:0461 interaction database SPSB4 EAF1 MI:0362 inference MI:0461 interaction database SPSB4 KLC2 MI:0362 inference MI:0461 interaction database SPSB4 DDX4 MI:0362 inference MI:0461 interaction database SPSB4 KLC4 MI:0362 inference MI:0461 interaction database SPSB4 RNF7 MI:0045 experimental interaction detection MI:0461 interaction database SPSB4 MAD2L2 MI:0362 inference MI:0461 interaction database SPSB4 AKT3 MI:0362 inference MI:0461 interaction database