PTM - CPLM Gene Name UniProt ID Type Position Code GRB2; ASH P62993 Acetylation 6 K GRB2; ASH P62993 Acetylation 38 K GRB2; ASH P62993 Acetylation 50 K GRB2; ASH P62993 Acetylation 109 K GRB2; ASH P62993 Ubiquitination 109 K GRB2; ASH P62993 Ubiquitination 20 K GRB2; ASH P62993 Ubiquitination 44 K GRB2; ASH P62993 Ubiquitination 56 K GRB2; ASH P62993 Ubiquitination 76 K GRB2; ASH P62993 Ubiquitination 69 K GRB2; ASH P62993 Ubiquitination 10 K

PTM - dbPAF Gene Name UniProt ID Position Type Species GRB2; ASH P62993 159 T Homo sapiens(Human) GRB2; ASH P62993 160 Y Homo sapiens(Human) GRB2; ASH P62993 18 S Homo sapiens(Human) GRB2; ASH P62993 209 Y Homo sapiens(Human) GRB2; ASH P62993 211 T Homo sapiens(Human) GRB2; ASH P62993 37 Y Homo sapiens(Human) GRB2; ASH P62993 52 Y Homo sapiens(Human) GRB2; ASH P62993 7 Y Homo sapiens(Human) GRB2; ASH P62993 88 S Homo sapiens(Human) GRB2; ASH P62993 90 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P62993 K6-ac Acetylation __MEAIAkyDFKATA P62993 K38-ac Acetylation ECDQNWykAELNGkD P62993 K50-ac Acetylation GkDGFIPkNyIEMkP P62993 K64-ac Acetylation PHPWFFGkIPRAkAE P62993 K109-ac Acetylation GNDVQHFkVLRDGAG P62993 K117-ac Acetylation VLRDGAGkYFLWVVK P62993 R78-m1 Methylation EEMLSkQrHDGAFLI P62993 R215-m1 Methylation NyVtPVNrNV_____ P62993 Y7-p Phosphorylation _MEAIAkyDFKATAD P62993 S18-p Phosphorylation ATADDELsFkRGDIL P62993 Y37-p Phosphorylation EECDQNWykAELNGk P62993 Y52-p Phosphorylation DGFIPkNyIEMkPHP P62993 S88-p Phosphorylation GAFLIREsEsAPGDF P62993 S90-p Phosphorylation FLIREsEsAPGDFSL P62993 T159-p Phosphorylation EQVPQQPtyVQALFD P62993 Y160-p Phosphorylation QVPQQPtyVQALFDF P62993 Y209-p Phosphorylation TGMFPRNyVtPVNrN P62993 T211-p Phosphorylation MFPRNyVtPVNrNV_ P62993 K20-ub Ubiquitination ADDELsFkRGDILKV P62993 K44-ub Ubiquitination ykAELNGkDGFIPkN P62993 K56-ub Ubiquitination PkNyIEMkPHPWFFG P62993 K69-ub Ubiquitination FGkIPRAkAEEMLSk P62993 K76-ub Ubiquitination kAEEMLSkQrHDGAF P62993 K109-ub Ubiquitination GNDVQHFkVLRDGAG

PTM - dbPTM UniProt ID Position Code Type Resources References P62993 6 K Acetylation Swiss-Prot 1010711 19608861 P62993 50 K Acetylation Swiss-Prot 1010711 19608861 P62993 109 K Acetylation Swiss-Prot 1010711 19608861 P62993 160 Y Phosphorylation Swiss-Prot 1010711 17081983 P62993 209 Y Phosphorylation Swiss-Prot 1010711 17081983 P62993 211 T Phosphorylation Swiss-Prot 1010711 18669648 P62993 1 M Acetylation Swiss-Prot 1010711 - P62993 211 T Phosphorylation PhosphoELM.10011 18669648 P62993 209 Y Phosphorylation PhosphoELM.10011 11726515 P62993 109 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P62993 20 K Ubiquitylation Phosphositeplus.1010730 21890473 P62993 44 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P62993 56 K Ubiquitylation Phosphositeplus.1010730 21890473 P62993 69 K Ubiquitylation Phosphositeplus.1010730 21906983 P62993 76 K Ubiquitylation Phosphositeplus.1010730 21906983 P62993 109 K Acetylation Phosphositeplus.1010730 19608861 P62993 38 K Acetylation Phosphositeplus.1010730 19608861 P62993 50 K Acetylation Phosphositeplus.1010730 19608861 P62993 6 K Acetylation Phosphositeplus.1010730 19608861 P62993 211 T Phosphorylation Phosphositeplus.1010730 18669648 P62993 160 Y Phosphorylation Phosphositeplus.1010730 20554525;20736484 P62993 209 Y Phosphorylation Phosphositeplus.1010730 11726515;18180459;19273609 P62993 37 Y Phosphorylation Phosphositeplus.1010730 19273609 P62993 52 Y Phosphorylation Phosphositeplus.1010730 19273609 P62993 7 Y Phosphorylation Phosphositeplus.1010730 19273609 P62993 6 K Acetylation HPRD 9.0 19608861 P62993 38 K Acetylation HPRD 9.0 19608861 P62993 50 K Acetylation HPRD 9.0 19608861 P62993 109 K Acetylation HPRD 9.0 19608861 P62993 211 T Phosphorylation HPRD 9.0 18669648 P62993 209 Y Phosphorylation HPRD 9.0 17081983 P62993 160 Y Phosphorylation (SRC) HPRD 9.0 9016807 P62993 32 C S-nitrosylation dbSNO 1.0 19483679 P62993 211 T Phosphorylation SysPTM 1.1 18669648 P62993 160 Y Phosphorylation SysPTM 1.1 17081983 P62993 209 Y Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References GRB2 109 K Acetylation 19608861 GRB2 119 Y Phosphorylation 9016807 GRB2 160 Y Phosphorylation 9016807 GRB2 168 Y Phosphorylation 11726515 GRB2 170 T Phosphorylation 18669648 GRB2 209 Y Phosphorylation 17081983 GRB2 211 T Phosphorylation 18669648 GRB2 38 K Acetylation 19608861 GRB2 50 K Acetylation 19608861 GRB2 6 K Acetylation 19608861 GRB2 68 K Acetylation 19608861

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P62993 211 T 18669648 HTP Phosphorylation P62993 209 Y 11726515 EGFR LTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P62993 1 N-acetylmethionine 22814378 P62993 6 N6-acetyllysine 19608861 P62993 50 N6-acetyllysine 19608861 P62993 109 N6-acetyllysine 19608861 P62993 211 Phosphothreonine 18669648

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type GRB2 IPI00021327 K 109 NDVQHFaKVLRDGA acetylation GRB2 IPI00021327 K 38 CDQNWYaKAELNGK acetylation GRB2 IPI00021327 K 50 KDGFIPaKNYIEMK acetylation GRB2 IPI00021327 K 6 _MEAIAaKYDFKAT acetylation GRB2 IPI00021327 Y 160 VPQQPTpYVQALFD phosphorylation GRB2 IPI00021327 Y 209 GMFPRNpYVTPVNR phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 2885 109142 - K Ubiquitination 21963094 2885 109142 - K Ubiquitination 21139048 2885 109142 - K Ubiquitination 21890473 2885 109142 - K Ubiquitination 21906983 2885 109142 - K Ubiquitination 23000965 2885 109142 - K Ubiquitination 24816145 2885 109142 - K Ubiquitination 25015289 2885 109142 - K Ubiquitination 19850743

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID GRB2 Homo sapiens (Human) 69; 20; 76; 109; 56; 44 "69,GKIPRAKAEEMLS; 20,DDELSFKRGDILK; 76,AEEMLSKQRHDGA; 109,NDVQHFKVLRDGA; 56,KNYIEMKPHPWFF; 44,KAELNGKDGFIPK" "69:21906983; 20:21890473; 76:21906983; 109:21890473; 56:21890473; 44:21906983,21890473" GRB2 Homo sapiens (Human) 68; 20; 44 "68,NDVQHFKVLRDGA; 20,DDELSFKRGDILK; 44,KAELNGKDGFIPK" "68:21890473; 20:21890473; 44:21890473,21906983" GRB2 Homo sapiens (Human) 69; 20; 76; 56; 109; 44 "69,GKIPRAKAEEMLS; 20,DDELSFKRGDILK; 76,AEEMLSKQRHDGA; 56,KNYIEMKPHPWFF; 109,NDVQHFKVLRDGA; 44,KAELNGKDGFIPK" "69:21906983; 20:21890473; 76:21906983; 56:21890473; 109:21890473,21906983; 44:21890473,21906983" GRB2 Homo sapiens (Human) 20; 56; 109; 44 "20,DDELSFKRGDILK; 56,KNYIEMKPHPWFF; 109,NDVQHFKVLRDGA; 44,KAELNGKDGFIPK" 20:21890473; 56:21890473; 109:21890473; 44:21890473 GRB2 Homo sapiens (Human) 109 "109,NDVQHFKVLRDGA" 109:21139048 GRB2 Homo sapiens (Human) 69; 20; 76; 109; 56; 44 "69,GKIPRAKAEEMLS; 20,DDELSFKRGDILK; 76,AEEMLSKQRHDGA; 109,NDVQHFKVLRDGA; 56,KNYIEMKPHPWFF; 44,KAELNGKDGFIPK" "69:21906983; 20:21890473; 76:21906983; 109:21906983,21890473; 56:21890473; 44:21890473"