PTM - CPLM Gene Name UniProt ID Type Position Code HCLS1; HS1 P14317 Acetylation 41 K HCLS1; HS1 P14317 Acetylation 123 K HCLS1; HS1 P14317 Acetylation 192 K HCLS1; HS1 P14317 Acetylation 241 K HCLS1; HS1 P14317 Ubiquitination 123 K HCLS1; HS1 P14317 Ubiquitination 60 K HCLS1; HS1 P14317 Ubiquitination 192 K

PTM - dbPAF Gene Name UniProt ID Position Type Species HCLS1; HS1 P14317 103 Y Homo sapiens(Human) HCLS1; HS1 P14317 124 Y Homo sapiens(Human) HCLS1; HS1 P14317 13 S Homo sapiens(Human) HCLS1; HS1 P14317 134 S Homo sapiens(Human) HCLS1; HS1 P14317 140 Y Homo sapiens(Human) HCLS1; HS1 P14317 153 Y Homo sapiens(Human) HCLS1; HS1 P14317 154 S Homo sapiens(Human) HCLS1; HS1 P14317 16 T Homo sapiens(Human) HCLS1; HS1 P14317 161 Y Homo sapiens(Human) HCLS1; HS1 P14317 175 Y Homo sapiens(Human) HCLS1; HS1 P14317 177 Y Homo sapiens(Human) HCLS1; HS1 P14317 181 T Homo sapiens(Human) HCLS1; HS1 P14317 186 S Homo sapiens(Human) HCLS1; HS1 P14317 190 Y Homo sapiens(Human) HCLS1; HS1 P14317 198 Y Homo sapiens(Human) HCLS1; HS1 P14317 208 S Homo sapiens(Human) HCLS1; HS1 P14317 219 T Homo sapiens(Human) HCLS1; HS1 P14317 220 T Homo sapiens(Human) HCLS1; HS1 P14317 222 Y Homo sapiens(Human) HCLS1; HS1 P14317 23 T Homo sapiens(Human) HCLS1; HS1 P14317 272 T Homo sapiens(Human) HCLS1; HS1 P14317 275 S Homo sapiens(Human) HCLS1; HS1 P14317 299 S Homo sapiens(Human) HCLS1; HS1 P14317 308 T Homo sapiens(Human) HCLS1; HS1 P14317 311 S Homo sapiens(Human) HCLS1; HS1 P14317 312 S Homo sapiens(Human) HCLS1; HS1 P14317 32 S Homo sapiens(Human) HCLS1; HS1 P14317 333 T Homo sapiens(Human) HCLS1; HS1 P14317 360 Y Homo sapiens(Human) HCLS1; HS1 P14317 378 Y Homo sapiens(Human) HCLS1; HS1 P14317 397 Y Homo sapiens(Human) HCLS1; HS1 P14317 42 T Homo sapiens(Human) HCLS1; HS1 P14317 481 Y Homo sapiens(Human) HCLS1; HS1 P14317 62 S Homo sapiens(Human) HCLS1; HS1 P14317 83 Y Homo sapiens(Human) HCLS1; HS1 P14317 97 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P14317 K41-ac Acetylation kEQRWGAktIEGSGR P14317 K123-ac Acetylation AAKGFGGkyGVERDR P14317 K141-ac Acetylation sAVGFDykGEVEKHT P14317 K192-ac Acetylation EsQRDyAkGFGGQyG P14317 K223-ac Acetylation EAPttAykkTTPIEA P14317 K224-ac Acetylation APttAykkTTPIEAA P14317 K241-ac Acetylation GTRGLkAkFESMAEE P14317 R155-m1 Methylation TSQKDysrGFGGRyG P14317 S13-p Phosphorylation VGHDVSVsVEtQGDD P14317 T16-p Phosphorylation DVSVsVEtQGDDWDt P14317 T23-p Phosphorylation tQGDDWDtDPDFVND P14317 S32-p Phosphorylation PDFVNDIsEkEQRWG P14317 T42-p Phosphorylation EQRWGAktIEGSGRT P14317 S62-p Phosphorylation HQLRNkVsEEHDVLR P14317 Y83-p Phosphorylation GPKASHGyGGRFGVE P14317 S97-p Phosphorylation ERDRMDKsAVGHEyV P14317 Y103-p Phosphorylation KsAVGHEyVAEVEKH P14317 Y124-p Phosphorylation AKGFGGkyGVERDRA P14317 S134-p Phosphorylation ERDRADKsAVGFDyk P14317 Y140-p Phosphorylation KsAVGFDykGEVEKH P14317 Y153-p Phosphorylation KHTSQKDysrGFGGR P14317 S154-p Phosphorylation HTSQKDysrGFGGRy P14317 Y161-p Phosphorylation srGFGGRyGVEKDKW P14317 Y175-p Phosphorylation WDKAALGyDyKGEtE P14317 Y177-p Phosphorylation KAALGyDyKGEtEKH P14317 T181-p Phosphorylation GyDyKGEtEKHEsQR P14317 S186-p Phosphorylation GEtEKHEsQRDyAkG P14317 Y190-p Phosphorylation KHEsQRDyAkGFGGQ P14317 Y198-p Phosphorylation AkGFGGQyGIQKDRV P14317 S208-p Phosphorylation QKDRVDKsAVGFNEM P14317 T219-p Phosphorylation FNEMEAPttAykkTT P14317 T220-p Phosphorylation NEMEAPttAykkTTP P14317 Y222-p Phosphorylation MEAPttAykkTTPIE P14317 S232-p Phosphorylation TTPIEAAsSGTRGLk P14317 T272-p Phosphorylation QQERKAVtKRsPEAP P14317 S275-p Phosphorylation RKAVtKRsPEAPQPV P14317 T308-p Phosphorylation EAWPPVGtPPSSESE P14317 T333-p Phosphorylation PLLPIRQtLPEDNEE P14317 Y360-p Phosphorylation QVEEEPVyEAEPEPE P14317 Y378-p Phosphorylation EPEPENDyEDVEEMD P14317 Y397-p Phosphorylation EDEPEGDyEEVLEPE P14317 Y481-p Phosphorylation FGLFPANyVKLLE__ P14317 K34-ub Ubiquitination FVNDIsEkEQRWGAk P14317 K60-ub Ubiquitination NIHQLRNkVsEEHDV P14317 K239-ub Ubiquitination sSGTRGLkAkFESMA

PTM - dbPTM UniProt ID Position Code Type Resources References P14317 41 K Acetylation Swiss-Prot 1010711 19608861 P14317 103 Y Phosphorylation Swiss-Prot 1010711 16094384 P14317 123 K Acetylation Swiss-Prot 1010711 19608861 P14317 192 K Acetylation Swiss-Prot 1010711 19608861 P14317 198 Y Phosphorylation Swiss-Prot 1010711 12522270 P14317 222 Y Phosphorylation Swiss-Prot 1010711 8611520;10066823 P14317 241 K Acetylation Swiss-Prot 1010711 19608861 P14317 275 S Phosphorylation Swiss-Prot 1010711 16094384;19690332 P14317 308 T Phosphorylation Swiss-Prot 1010711 19690332 P14317 275 S Phosphorylation PhosphoELM.10011 16094384 P14317 103 Y Phosphorylation PhosphoELM.10011 16094384 P14317 198 Y Phosphorylation PhosphoELM.10011 15144186;12522270 P14317 360 Y Phosphorylation PhosphoELM.10011 8578591 P14317 222 Y Phosphorylation PhosphoELM.10011 10066823;10066823 P14317 397 Y Phosphorylation PhosphoELM.10011 8578591;9104825;9183008;9104825 P14317 378 Y Phosphorylation PhosphoELM.10011 8578591;9104825;9104825 P14317 123 K Acetylation Phosphositeplus.1010730 19608861 P14317 192 K Acetylation Phosphositeplus.1010730 19608861 P14317 241 K Acetylation Phosphositeplus.1010730 19608861 P14317 41 K Acetylation Phosphositeplus.1010730 19608861 P14317 275 S Phosphorylation Phosphositeplus.1010730 16094384;19690332;20164059;20562096;22115753 P14317 16 T Phosphorylation Phosphositeplus.1010730 10806407 P14317 23 T Phosphorylation Phosphositeplus.1010730 10806407 P14317 272 T Phosphorylation Phosphositeplus.1010730 22115753 P14317 308 T Phosphorylation Phosphositeplus.1010730 19690332;22115753 P14317 103 Y Phosphorylation Phosphositeplus.1010730 15570572;16094384 P14317 175 Y Phosphorylation Phosphositeplus.1010730 21394647 P14317 177 Y Phosphorylation Phosphositeplus.1010730 21394647 P14317 198 Y Phosphorylation Phosphositeplus.1010730 12522270;14729942;15144186;15570572;18083107;21394647 P14317 222 Y Phosphorylation Phosphositeplus.1010730 10066823;15795233;20562096;22679023 P14317 378 Y Phosphorylation Phosphositeplus.1010730 16782030;18587398;22679023;9104825 P14317 397 Y Phosphorylation Phosphositeplus.1010730 15795233;16782030;18587398;22679023;9104825 P14317 41 K Acetylation HPRD 9.0 19608861 P14317 123 K Acetylation HPRD 9.0 19608861 P14317 192 K Acetylation HPRD 9.0 19608861 P14317 241 K Acetylation HPRD 9.0 19608861 P14317 275 S Phosphorylation HPRD 9.0 16094384 P14317 103 Y Phosphorylation HPRD 9.0 15570572 P14317 198 Y Phosphorylation HPRD 9.0 12522270,15144186 P14317 16 T Phosphorylation (CSNK2A1) HPRD 9.0 10806407 P14317 23 T Phosphorylation (CSNK2A1) HPRD 9.0 10806407 P14317 222 Y Phosphorylation (FGR) HPRD 9.0 10066823 P14317 222 Y Phosphorylation (LYN) HPRD 9.0 10066823 P14317 378 Y Phosphorylation (LYN) HPRD 9.0 9104825 P14317 397 Y Phosphorylation (LYN) HPRD 9.0 9104825 P14317 378 Y Phosphorylation (SYK) HPRD 9.0 9104825 P14317 397 Y Phosphorylation (SYK) HPRD 9.0 9104825

PTM - HPRD Symbol Position Code Type References HCLS1 103 Y Phosphorylation 15570572 HCLS1 123 K Acetylation 19608861 HCLS1 16 T Phosphorylation 10806407 HCLS1 192 K Acetylation 19608861 HCLS1 198 Y Phosphorylation 12522270,15144186 HCLS1 222 Y Phosphorylation 10066823 HCLS1 23 T Phosphorylation 10806407 HCLS1 241 K Acetylation 19608861 HCLS1 275 S Phosphorylation 16094384 HCLS1 378 Y Phosphorylation 9104825 HCLS1 397 Y Phosphorylation 9104825 HCLS1 41 K Acetylation 19608861

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P14317 198 Y 15144186 HTP Phosphorylation P14317 198 Y 12522270 HTP Phosphorylation P14317 378 Y 8578591 LTP Phosphorylation P14317 378 Y 9104825 Lyn LTP Phosphorylation P14317 378 Y 9104825 SYK LTP Phosphorylation P14317 103 Y 16094384 HTP Phosphorylation P14317 360 Y 8578591 LTP Phosphorylation P14317 222 Y 10066823 Lyn LTP Phosphorylation P14317 222 Y 10066823 Fgr LTP Phosphorylation P14317 397 Y 8578591 LTP Phosphorylation P14317 397 Y 9104825 Lyn LTP Phosphorylation P14317 397 Y 9183008 Fgr LTP Phosphorylation P14317 397 Y 9104825 SYK LTP Phosphorylation P14317 275 S 16094384 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P14317 41 N6-acetyllysine 19608861 P14317 123 N6-acetyllysine 19608861 P14317 192 N6-acetyllysine 19608861 P14317 198 Phosphotyrosine 12522270;15144186 P14317 222 Phosphotyrosine; by FGR 10066823;8611520 P14317 241 N6-acetyllysine 19608861 P14317 275 Phosphoserine 19690332;23186163;24275569 P14317 308 Phosphothreonine 19690332;23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type HCLS1 IPI00026156 K 123 AKGFGGaKYGVERD acetylation HCLS1 IPI00026156 K 192 SQRDYAaKGFGGQY acetylation HCLS1 IPI00026156 K 241 ARGLKAaKFESMAE acetylation HCLS1 IPI00026156 K 41 EQRWGAaKTIEGSG acetylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 3059 109309 - K Ubiquitination 24816145

PTM - PhosphoBase Gene Name PB Accession PROT ID Serine Threonine Tyrosine Peptide HCLS1;HS1 A010 Hematopoietic lineage cell specific protein - - 360 [A010-A], 378 [A010-B], 397 [A010-C] A010-C - PEGDYEEVL;!