PTM - CPLM Gene Name UniProt ID Type Position Code LCK P06239 Acetylation 179 K LCK P06239 Ubiquitination 379 K LCK P06239 Ubiquitination 246 K LCK P06239 Ubiquitination 405 K LCK P06239 Ubiquitination 269 K

PTM - dbPAF Gene Name UniProt ID Position Type Species LCK P06239 102 S Homo sapiens(Human) LCK P06239 121 S Homo sapiens(Human) LCK P06239 133 S Homo sapiens(Human) LCK P06239 156 S Homo sapiens(Human) LCK P06239 158 S Homo sapiens(Human) LCK P06239 159 T Homo sapiens(Human) LCK P06239 162 S Homo sapiens(Human) LCK P06239 164 S Homo sapiens(Human) LCK P06239 181 Y Homo sapiens(Human) LCK P06239 192 Y Homo sapiens(Human) LCK P06239 194 S Homo sapiens(Human) LCK P06239 198 T Homo sapiens(Human) LCK P06239 209 Y Homo sapiens(Human) LCK P06239 210 T Homo sapiens(Human) LCK P06239 213 S Homo sapiens(Human) LCK P06239 25 Y Homo sapiens(Human) LCK P06239 263 Y Homo sapiens(Human) LCK P06239 264 Y Homo sapiens(Human) LCK P06239 268 T Homo sapiens(Human) LCK P06239 274 S Homo sapiens(Human) LCK P06239 281 S Homo sapiens(Human) LCK P06239 35 T Homo sapiens(Human) LCK P06239 375 T Homo sapiens(Human) LCK P06239 394 Y Homo sapiens(Human) LCK P06239 395 T Homo sapiens(Human) LCK P06239 414 Y Homo sapiens(Human) LCK P06239 416 T Homo sapiens(Human) LCK P06239 42 S Homo sapiens(Human) LCK P06239 470 Y Homo sapiens(Human) LCK P06239 489 Y Homo sapiens(Human) LCK P06239 492 S Homo sapiens(Human) LCK P06239 50 T Homo sapiens(Human) LCK P06239 501 T Homo sapiens(Human) LCK P06239 505 Y Homo sapiens(Human) LCK P06239 51 Y Homo sapiens(Human) LCK P06239 54 S Homo sapiens(Human) LCK P06239 59 S Homo sapiens(Human) LCK P06239 71 S Homo sapiens(Human) LCK P06239 94 S Homo sapiens(Human)

PTM - PhosSNP Gene Name dbSNP ID Gene Mutation AA Mutation Mutation Position Code Peptide Score Modification Type LCK rs61781208 TAC->CAC Y->H 414 Y TAPEAINYGTFTIKS 414 Phosphorylation TypeI- LCK rs61781208 TAC->CAC Y->H 414 Y TAPEAINYGTFTIKS 414 Phosphorylation TypeI- LCK rs11576032 CGG->TGG R->W 162 S ESESTAGSFSLSVRD 162 Phosphorylation TypeIII- LCK rs11576032 CGG->TGG R->W 162 S ESESTAGSFSLSVRD 162 Phosphorylation TypeIII-

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P06239 K179-ac Acetylation QNQGEVVkHyKIRNL P06239 Y25-p Phosphorylation DVCENCHyPIVPLDG P06239 T35-p Phosphorylation VPLDGKGtLLIRNGs P06239 S42-p Phosphorylation tLLIRNGsEVRDPLV P06239 T50-p Phosphorylation EVRDPLVtyEGSNPP P06239 Y51-p Phosphorylation VRDPLVtyEGSNPPA P06239 S59-p Phosphorylation EGSNPPAsPLQDNLV P06239 S94-p Phosphorylation QLRILEQsGEWWKAQ P06239 S102-p Phosphorylation GEWWKAQsLTTGQEG P06239 S121-p Phosphorylation NFVAKANsLEPEPWF P06239 S133-p Phosphorylation PWFFkNLsRKDAERQ P06239 S150-p Phosphorylation APGNTHGsFLIREsE P06239 S156-p Phosphorylation GsFLIREsEstAGsF P06239 S158-p Phosphorylation FLIREsEstAGsFSL P06239 T159-p Phosphorylation LIREsEstAGsFSLS P06239 S162-p Phosphorylation EsEstAGsFSLSVRD P06239 Y181-p Phosphorylation QGEVVkHyKIRNLDN P06239 Y192-p Phosphorylation NLDNGGFyIsPRItF P06239 S194-p Phosphorylation DNGGFyIsPRItFPG P06239 T198-p Phosphorylation FyIsPRItFPGLHEL P06239 Y209-p Phosphorylation LHELVRHytNAsDGL P06239 T210-p Phosphorylation HELVRHytNAsDGLC P06239 S213-p Phosphorylation VRHytNAsDGLCTRL P06239 Y263-p Phosphorylation FGEVWMGyyNGHtkV P06239 Y264-p Phosphorylation GEVWMGyyNGHtkVA P06239 T268-p Phosphorylation MGyyNGHtkVAVksL P06239 S274-p Phosphorylation HtkVAVksLkQGSMs P06239 S281-p Phosphorylation sLkQGSMsPDAFLAE P06239 T375-p Phosphorylation ANILVSDtLSCkIAD P06239 Y394-p Phosphorylation RLIEDNEytAREGAk P06239 T395-p Phosphorylation LIEDNEytAREGAkF P06239 Y414-p Phosphorylation TAPEAINyGtFTIKS P06239 T416-p Phosphorylation PEAINyGtFTIKSDV P06239 Y470-p Phosphorylation DNCPEELyQLMRLCW P06239 Y489-p Phosphorylation EDRPTFDyLRsVLED P06239 S492-p Phosphorylation PTFDyLRsVLEDFFT P06239 T501-p Phosphorylation LEDFFTAtEGQyQPQ P06239 Y505-p Phosphorylation FTAtEGQyQPQP___ P06239 K84-ub Ubiquitination DGDLGFEkGEQLRIL P06239 K130-ub Ubiquitination EPEPWFFkNLsRKDA P06239 K179-ub Ubiquitination QNQGEVVkHyKIRNL P06239 K246-ub Ubiquitination EVPRETLkLVERLGA P06239 K269-ub Ubiquitination GyyNGHtkVAVksLk P06239 K273-ub Ubiquitination GHtkVAVksLkQGSM P06239 K276-ub Ubiquitination kVAVksLkQGSMsPD P06239 K293-ub Ubiquitination LAEANLMkQLQHQRL P06239 K379-ub Ubiquitination VSDtLSCkIADFGLA P06239 K401-ub Ubiquitination ytAREGAkFPIkWTA P06239 K405-ub Ubiquitination EGAkFPIkWTAPEAI P06239 K478-ub Ubiquitination QLMRLCWkERPEDRP

PTM - dbPTM UniProt ID Position Code Type Resources References P06239 102 S Phosphorylation Swiss-Prot 1010711 19690332 P06239 159 T Phosphorylation Swiss-Prot 1010711 19690332 P06239 162 S Phosphorylation Swiss-Prot 1010711 17192257;19690332 P06239 179 K Acetylation Swiss-Prot 1010711 19608861 P06239 192 Y Phosphorylation Swiss-Prot 1010711 17192257;18083107;19690332 P06239 194 S Phosphorylation Swiss-Prot 1010711 19690332 P06239 213 S Phosphorylation Swiss-Prot 1010711 17192257 P06239 394 Y Phosphorylation Swiss-Prot 1010711 8139546;15592455;17192257 P06239 501 T Phosphorylation Swiss-Prot 1010711 17192257 P06239 505 Y Phosphorylation Swiss-Prot 1010711 1639064;8139546;8631775;15592455;17192257;18083107;18691976;19369195;19690332 P06239 59 S Phosphorylation PhosphoELM.10011 15199125 P06239 158 S Phosphorylation PhosphoELM.10011 8262984 P06239 162 S Phosphorylation PhosphoELM.10011 17192257 P06239 194 S Phosphorylation PhosphoELM.10011 8262984 P06239 213 S Phosphorylation PhosphoELM.10011 17192257 P06239 42 S Phosphorylation PhosphoELM.10011 8506364;8226850;8506364;8226850 P06239 501 T Phosphorylation PhosphoELM.10011 17192257 P06239 192 Y Phosphorylation PhosphoELM.10011 18083107;17192257;15659558 P06239 394 Y Phosphorylation PhosphoELM.10011 17192257;15659558;15592455;3265417 P06239 505 Y Phosphorylation PhosphoELM.10011 18083107;17192257;15592455;3265417;8945479 P06239 179 K Acetylation Phosphositeplus.1010730 19608861 P06239 102 S Phosphorylation Phosphositeplus.1010730 19690332 P06239 158 S Phosphorylation Phosphositeplus.1010730 19690332 P06239 162 S Phosphorylation Phosphositeplus.1010730 17192257;19690332 P06239 194 S Phosphorylation Phosphositeplus.1010730 19690332 P06239 42 S Phosphorylation Phosphositeplus.1010730 20164059;21406692 P06239 159 T Phosphorylation Phosphositeplus.1010730 19690332 P06239 395 T Phosphorylation Phosphositeplus.1010730 17192257;22115753 P06239 501 T Phosphorylation Phosphositeplus.1010730 17192257 P06239 192 Y Phosphorylation Phosphositeplus.1010730 15659558;16493040;16497976;17192257;18083107;18180459;19605366;19664994;19690332;20164059;20736484;21253578;21394647;22322096 P06239 394 Y Phosphorylation Phosphositeplus.1010730 15570572;15592455;15659558;15951569;16111633;16461343;16464493;16493040;17192257;17360941;17389760;18056643;18083107;18180459;18541536;18578522;18669648;18691976;18707149;18776048;19369195;19426503;19534553;19605366;19658100;19664994;19690332;20058876;20068231;20090780;20541955;20736484;21049007;21253578;21394647;21403953;21406692;21447384;21659604;22115753;22322096 P06239 505 Y Phosphorylation Phosphositeplus.1010730 15570572;15592455;15743820;15917250;16461343;16493040;16497976;17192257;18070987;18083107;18180459;18691976;19369195;19605366;19690332;19858202;19901323;20541955;21253578;21394647;21406692;22115753;22322096;2503711 P06239 179 K Acetylation HPRD 9.0 19608861 P06239 394 Y Dephosphorylation HPRD 9.0 16461343 P06239 394 Y Dephosphorylation HPRD 9.0 11294838 P06239 394 Y Dephosphorylation HPRD 9.0 12589038,11369760,10330160,9973453 P06239 505 Y Dephosphorylation HPRD 9.0 8663450,12589038,11369760,10330160,9973453 P06239 2 G Myristoylation HPRD 9.0 10975838 P06239 3 C Palmitoylation HPRD 9.0 9092949 P06239 5 C Palmitoylation HPRD 9.0 9092949 P06239 133 S Phosphorylation HPRD 9.0 18452278 P06239 192 Y Phosphorylation HPRD 9.0 18083107,16497976,19664994 P06239 394 Y Phosphorylation HPRD 9.0 16461343,18578522 P06239 394 Y Phosphorylation HPRD 9.0 8945479,8258691,8405050,15144186,18578522 P06239 505 Y Phosphorylation HPRD 9.0 16461343,16497976,19901323,18691976 P06239 505 Y Phosphorylation HPRD 9.0 12485116,15144186,16497976,19901323,18691976 P06239 59 S Phosphorylation (MAPK1) HPRD 9.0 8506364,8618896 P06239 59 S Phosphorylation (MAPK3) HPRD 9.0 8506364,8618896 P06239 42 S Phosphorylation (PRKACA) HPRD 9.0 8506364 P06239 42 S Phosphorylation (PRKCA) HPRD 9.0 8506364 P06239 505 Y Phosphorylation (CSK) HPRD 9.0 12485116,15144186,16497976,19901323,18691976 P06239 394 Y Phosphorylation (FCGR3A) HPRD 9.0 8945479,8258691,8405050,15144186,18578522 P06239 394 Y Phosphorylation (LCK) HPRD 9.0 8945479,8258691,8405050,15144186,18578522 P06239 192 Y Phosphorylation (SYK) HPRD 9.0 8798764,16497976,19664994 P06239 394 Y Phosphorylation SysPTM 1.1 15951569

PTM - HPRD Symbol Position Code Type References LCK 133 S Phosphorylation 18452278 LCK 179 K Acetylation 19608861 LCK 192 Y Phosphorylation 18083107,16497976,19664994 LCK 192 Y Phosphorylation 8798764,16497976,19664994 LCK 2 G Myristoylation 10975838 LCK 3 C Palmitoylation 9092949 LCK 393 Y Phosphorylation 17016520 LCK 394 Y Dephosphorylation 11294838 LCK 394 Y Dephosphorylation 12589038,11369760,10330160,9973453 LCK 394 Y Dephosphorylation 16461343 LCK 394 Y Phosphorylation 16461343,18578522 LCK 394 Y Phosphorylation 8945479,8258691,8405050,15144186,18578522 LCK 42 S Phosphorylation 8506364 LCK 5 C Palmitoylation 9092949 LCK 505 Y Dephosphorylation 8663450,12589038,11369760,10330160,9973453 LCK 505 Y Phosphorylation 12485116,15144186,16497976,19901323,18691976 LCK 505 Y Phosphorylation 16461343,16497976,19901323,18691976 LCK 59 S Phosphorylation 8506364,8618896

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P06239 394 Y 17192257 HTP Phosphorylation P06239 394 Y 15659558 HTP Phosphorylation P06239 394 Y 15592455 HTP Phosphorylation P06239 394 Y 3265417 Lck LTP Phosphorylation P06239 501 T 17192257 HTP Phosphorylation P06239 213 S 17192257 HTP Phosphorylation P06239 505 Y 18083107 HTP Phosphorylation P06239 505 Y 17192257 HTP Phosphorylation P06239 505 Y 15592455 HTP Phosphorylation P06239 505 Y 3265417 LTP Phosphorylation P06239 505 Y 8945479 Lck LTP Phosphorylation P06239 194 S 8262984 LTP Phosphorylation P06239 59 S 15199125 LTP Phosphorylation P06239 192 Y 18083107 HTP Phosphorylation P06239 192 Y 17192257 HTP Phosphorylation P06239 192 Y 15659558 HTP Phosphorylation P06239 42 S 8506364 MAPK3 LTP Phosphorylation P06239 42 S 8226850 MAPK3 LTP Phosphorylation P06239 42 S 8506364 MAPK1 LTP Phosphorylation P06239 42 S 8226850 MAPK1 LTP Phosphorylation P06239 158 S 8262984 LTP Phosphorylation P06239 162 S 17192257 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P06239 102 Phosphoserine 19690332 P06239 159 Phosphothreonine 19690332 P06239 162 Phosphoserine 19690332 P06239 194 Phosphoserine 19690332 P06239 394 Phosphotyrosine; by autocatalysis 8139546 P06239 505 Phosphotyrosine; by CSK 15592455;18691976;19369195;19690332;21406692;1639064;8139546;8631775

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type LCK IPI00394952 K 179 NQGEVVaKHYKIRN acetylation LCK IPI00394952 Y 505 TATEGQpYQPQP__ phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 3932 110124 - K Ubiquitination 12746907 3932 110124 - K Ubiquitination 20007709 3932 110124 - K Ubiquitination 22505724 3932 110124 - K Ubiquitination 24816145 3932 110124 - K Ubiquitination 19850743

PTM - PhosphoBase Gene Name PB Accession PROT ID Serine Threonine Tyrosine Peptide LCK A030 proto-oncogene tyrosine-protein kinase Lck (p56-Lck) (T cell-specific protein-tyrosine kinase) - - 393 [A030-A]..[A030-A01], 504 [A030-B] A030-A01 389-401 PRLIEDAEYAARG;MUTATION 389(A->P);MUTATION 395(N->A);MUTATION 398(T->A);MUTATION 401(E->G);!