PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus FZD6 ZNRF3 22575959 dip exp GP GP P GP G GP GP G GP P GP G G GP GP GP GP G GP GP GP G GP GP GP GP G UBE2N ZNRF3 19690564 biogrid,intact exp GP GP G P GP G GP GP G GP GP GP GP G GP GP G GP G GP GP GP G GP GP GP GP G ZNRF3 FZD4 22575959 biogrid exp GP GP G P GP G GP GP G GP GP GP GP G GP GP G GP G GP GP GP G GP G GP GP G HSPB1 ZNRF3 25277244 biogrid,intact exp GP GP G P G G GP GP G GP GP GP G G GP G GP GP G GP GP GP G GP G GP GP G RSPO1 ZNRF3 22575959,24050775,24165923 biogrid,intact,dip exp P P P P P P P P G P P P P P P P P P P G DVL2 ZNRF3 25891077 biogrid exp GP GP G P GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G RSPO3 ZNRF3 24050775 biogrid exp GP GP G P GP G GP GP G GP G GP GP G GP GP G GP G GP GP GP G GP GP GP GP G LGR4 ZNRF3 22575959,24639526,24165923 biogrid,dip exp GP GP G P GP G GP GP G GP G P P G GP GP G GP G GP GP GP G GP GP GP GP G GFI1B ZNRF3 16713569 hprd,biogrid,mint,intact exp P P P P P P P P P P P P P P P P P LRP6 ZNRF3 22575959 biogrid,dip exp GP GP G P GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G RSPO2 ZNRF3 24050775 biogrid exp, ortho P P G P GP G P P P P P P P GP P P P P P GP P ZNRF3 ZNRF3 ortho GP GP G P GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B LGR4 ZNRF3 MI:0004(affinity chromatography technology)|MI:0096(pull down) 22575959,24165923 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid) BIOGRID_199599|BIOGRID_293790 prey|prey bait|bait RSPO1 ZNRF3 MI:0004(affinity chromatography technology)|MI:0096(pull down)|MI:0004(affinity chromatography technology)|MI:0096(pull down) 22575959,24165923 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid)|(biogrid)|(biogrid) BIOGRID_199600|BIOGRID_293789|BIOGRID_199598|BIOGRID_293791 prey|bait|prey|prey bait|prey|bait|bait UBE2N ZNRF3 MI:0018(two hybrid)|MI:0397(two hybrid array) 19690564 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_123631|UniProt_102620 bait|bait prey|prey GFI1B ZNRF3 MI:0018(two hybrid)|MI:0096(pull down)|MI:0018(two hybrid)|MI:0397(two hybrid array)|MI:0096(pull down) 16713569 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) -|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association) MI:0468(hprd)|MI:0471(mint)|(biogrid)|(intact)|MI:0471(mint) HPRD_33061|MINT_50875|BIOGRID_215865|UniProt_86430|MINT_83163 -|bait|bait|bait|bait -|prey|prey|prey|prey LRP6 ZNRF3 MI:0004(affinity chromatography technology) 22575959 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_199603 bait prey ZNRF3 FZD4 MI:0004(affinity chromatography technology) 22575959 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_199597 prey bait

PPI - HINT Gene A Gene B PMID Method Quality RSPO1 ZNRF3 22575959,25504990,22575959,22575959,22575959,24165923,24165923,24165923 0096,0096,0007,0428,0004,0107,0004,0096 LC LGR4 ZNRF3 24165923,22575959,24639526,22575959 0004,0007,0004,0004 LC RSPO1 ZNRF3 22575959,25504990,22575959,22575959,22575959,24165923,24165923,24165923 0096,0096,0007,0428,0004,0107,0004,0096 LC LGR4 ZNRF3 24165923,22575959,24639526,22575959 0004,0007,0004,0004 LC LGR5 ZNRF3 26123262 0114 LC HSPB1 ZNRF3 25277244 0097 LC UBE2N ZNRF3 19690564,19690564 0397,0018 HT RSPO1 ZNRF3 24050775,24165923,24165923,24165923 0055,0107,0004,0096 LC GFI1B ZNRF3 16713569 0018 HT RSPO2 ZNRF3 24050775 0055 LC RSPO3 ZNRF3 24050775 0055 LC ZNRF3 HEL-S-71 19690564 0018 HT ZNRF3 HEL-S-102 25277244 0018 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q9ULT6 O75473 ZNRF3 LGR5 0.309 26123262 Q9ULT6 O75581 ZNRF3 LRP6 0.21 22575959 Q9ULT6 P04792 ZNRF3 HSPB1 0.286 25277244 Q9ULT6 P61088 ZNRF3 UBE2N 0.286 19690564 Q9ULT6 Q2MKA7 ZNRF3 RSPO1 0.914 24165923,24050775,22575959,25504990 Q9ULT6 Q9BXB1 ZNRF3 LGR4 0.454 24165923,24639526,22575959 Q9ULT6 Q9BXY4 ZNRF3 RSPO3 0.523 25504990,24050775 Q9ULT6 O14641 ZNRF3 DVL2 0.21 25891077 Q9ULT6 Q5VTD9 ZNRF3 GFI1B 0.454 16713569 Q9ULT6 Q6UXX9 ZNRF3 RSPO2 0.523 25504990,24050775 Q9ULV1 Q9ULT6 FZD4 ZNRF3 0.21 22575959

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 DVL2 ZNRF3 MI:0045 experimental interaction detection MI:0461 interaction database FZD6 ZNRF3 MI:0362 inference MI:0461 interaction database LGR5 ZNRF3 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database LRP6 ZNRF3 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database HSPB1 ZNRF3 MI:0045 experimental interaction detection MI:0461 interaction database UBB ZNRF3 MI:0362 inference MI:1106 pathways database UBC ZNRF3 MI:0362 inference MI:1106 pathways database WNT3A ZNRF3 MI:0362 inference MI:1106 pathways database UBE2N ZNRF3 MI:0045 experimental interaction detection MI:0461 interaction database RPS27A ZNRF3 MI:0362 inference MI:1106 pathways database UBA52 ZNRF3 MI:0362 inference MI:1106 pathways database FZD5 ZNRF3 MI:0362 inference MI:0461 interaction database RSPO4 ZNRF3 MI:0362 inference MI:1106 pathways database RSPO1 ZNRF3 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database GFI1B ZNRF3 MI:0045 experimental interaction detection MI:0461 interaction database RNF43 ZNRF3 MI:0362 inference MI:1106 pathways database RSPO2 ZNRF3 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database LGR4 ZNRF3 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database RSPO3 ZNRF3 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database FZD8 ZNRF3 MI:0362 inference MI:0461 interaction database LGR6 ZNRF3 MI:0362 inference MI:1106 pathways database ZNRF3 FZD4 MI:0045 experimental interaction detection MI:0461,MI:1106 interaction database,pathways database