PTM - CPLM Gene Name UniProt ID Type Position Code TBK1; NAK Q9UHD2 Acetylation 584 K TBK1; NAK Q9UHD2 Ubiquitination 154 K TBK1; NAK Q9UHD2 Ubiquitination 396 K TBK1; NAK Q9UHD2 Ubiquitination 241 K TBK1; NAK Q9UHD2 Ubiquitination 251 K TBK1; NAK Q9UHD2 Ubiquitination 504 K TBK1; NAK Q9UHD2 Ubiquitination 692 K TBK1; NAK Q9UHD2 Ubiquitination 372 K TBK1; NAK Q9UHD2 Ubiquitination 231 K TBK1; NAK Q9UHD2 Ubiquitination 484 K TBK1; NAK Q9UHD2 Ubiquitination 416 K TBK1; NAK Q9UHD2 Ubiquitination 661 K TBK1; NAK Q9UHD2 Ubiquitination 670 K TBK1; NAK Q9UHD2 Ubiquitination 137 K TBK1; NAK Q9UHD2 Ubiquitination 344 K TBK1; NAK Q9UHD2 Ubiquitination 584 K

PTM - dbPAF Gene Name UniProt ID Position Type Species TBK1; NAK Q9UHD2 151 S Homo sapiens(Human) TBK1; NAK Q9UHD2 153 Y Homo sapiens(Human) TBK1; NAK Q9UHD2 172 S Homo sapiens(Human) TBK1; NAK Q9UHD2 174 Y Homo sapiens(Human) TBK1; NAK Q9UHD2 179 Y Homo sapiens(Human) TBK1; NAK Q9UHD2 247 S Homo sapiens(Human) TBK1; NAK Q9UHD2 325 Y Homo sapiens(Human) TBK1; NAK Q9UHD2 329 Y Homo sapiens(Human) TBK1; NAK Q9UHD2 340 Y Homo sapiens(Human) TBK1; NAK Q9UHD2 354 Y Homo sapiens(Human) TBK1; NAK Q9UHD2 503 T Homo sapiens(Human) TBK1; NAK Q9UHD2 509 S Homo sapiens(Human) TBK1; NAK Q9UHD2 510 S Homo sapiens(Human) TBK1; NAK Q9UHD2 511 S Homo sapiens(Human) TBK1; NAK Q9UHD2 514 T Homo sapiens(Human) TBK1; NAK Q9UHD2 527 S Homo sapiens(Human) TBK1; NAK Q9UHD2 531 S Homo sapiens(Human) TBK1; NAK Q9UHD2 577 Y Homo sapiens(Human) TBK1; NAK Q9UHD2 664 T Homo sapiens(Human) TBK1; NAK Q9UHD2 672 T Homo sapiens(Human) TBK1; NAK Q9UHD2 716 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9UHD2 S151-p Phosphorylation VIGEDGQsVykLTDF Q9UHD2 Y153-p Phosphorylation GEDGQsVykLTDFGA Q9UHD2 S172-p Phosphorylation EDDEQFVsLyGTEEy Q9UHD2 Y174-p Phosphorylation DEQFVsLyGTEEyLH Q9UHD2 Y179-p Phosphorylation sLyGTEEyLHPDMYE Q9UHD2 S247-p Phosphorylation GkPSGAIsGVQKAEN Q9UHD2 T278-p Phosphorylation RGLQVLLtPVLANIL Q9UHD2 Y325-p Phosphorylation QMTAHKIyIHSYNTA Q9UHD2 Y340-p Phosphorylation TIFHELVyKQTkIIS Q9UHD2 Y354-p Phosphorylation SSNQELIyEGRRLVL Q9UHD2 Y394-p Phosphorylation LNTIGLIyEkISLPk Q9UHD2 Y435-p Phosphorylation IASTLLLyQELMRKG Q9UHD2 T503-p Phosphorylation GEISDIHtkLLRLSS Q9UHD2 S511-p Phosphorylation kLLRLSSsQGTIETS Q9UHD2 S527-p Phosphorylation QDIDSRLsPGGSLAD Q9UHD2 Y577-p Phosphorylation KAERRLAyNEEQIHk Q9UHD2 Y591-p Phosphorylation kFDKQKLyyHATKAM Q9UHD2 Y592-p Phosphorylation FDKQKLyyHATKAMT Q9UHD2 Y647-p Phosphorylation IEEEVSKyQEyTNEL Q9UHD2 Y650-p Phosphorylation EVSKyQEyTNELQET Q9UHD2 T664-p Phosphorylation TLPQkMFtASSGIKH Q9UHD2 Y677-p Phosphorylation KHTMTPIyPSSNTLV Q9UHD2 S716-p Phosphorylation HILERFGsLTMDGGL Q9UHD2 K30-ub Ubiquitination VFRGRHKkTGDLFAI Q9UHD2 K69-ub Ubiquitination LNHKNIVkLFAIEEE Q9UHD2 K137-ub Ubiquitination GIVHRDIkPGNIMRV Q9UHD2 K154-ub Ubiquitination EDGQsVykLTDFGAA Q9UHD2 K241-ub Ubiquitination MYKIITGkPSGAIsG Q9UHD2 K291-ub Ubiquitination ILEADQEkCWGFDQF Q9UHD2 K344-ub Ubiquitination ELVyKQTkIISSNQE Q9UHD2 K372-ub Ubiquitination RLAQHFPkTTEENPI Q9UHD2 K396-ub Ubiquitination TIGLIyEkISLPkVH Q9UHD2 K401-ub Ubiquitination yEkISLPkVHPRYDL Q9UHD2 K504-ub Ubiquitination EISDIHtkLLRLSSs Q9UHD2 K584-ub Ubiquitination yNEEQIHkFDKQKLy Q9UHD2 K661-ub Ubiquitination LQETLPQkMFtASSG

PTM - dbPTM UniProt ID Position Code Type Resources References Q9UHD2 172 S Phosphorylation Swiss-Prot 1010711 11839743;19369195 Q9UHD2 509 S Phosphorylation Swiss-Prot 1010711 19369195 Q9UHD2 510 S Phosphorylation Swiss-Prot 1010711 19369195 Q9UHD2 670 K Ubiquitylation Swiss-Prot 1010711 22388039 Q9UHD2 716 S Phosphorylation Swiss-Prot 1010711 18691976;19369195 Q9UHD2 172 S Phosphorylation PhosphoELM.10011 11839743 Q9UHD2 154 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHD2 241 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHD2 344 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHD2 396 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHD2 504 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHD2 584 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHD2 661 K Ubiquitylation Phosphositeplus.1010730 21906983 Q9UHD2 172 S Phosphorylation Phosphositeplus.1010730 11839743;18356163;19369195;21145761;22322096 Q9UHD2 716 S Phosphorylation Phosphositeplus.1010730 18691976;19369195;21145761 Q9UHD2 153 Y Phosphorylation Phosphositeplus.1010730 20736484 Q9UHD2 354 Y Phosphorylation Phosphositeplus.1010730 20090780 Q9UHD2 577 Y Phosphorylation Phosphositeplus.1010730 18083107 Q9UHD2 584 K Acetylation HPRD 9.0 19608861 Q9UHD2 172 S Phosphorylation HPRD 9.0 11839743 Q9UHD2 509 S Phosphorylation HPRD 9.0 18691976 Q9UHD2 716 S Phosphorylation HPRD 9.0 18691976 Q9UHD2 672 T Phosphorylation HPRD 9.0 18691976 Q9UHD2 716 S Phosphorylation SysPTM 1.1 18691976

PTM - HPRD Symbol Position Code Type References TBK1 172 S Phosphorylation 11839743 TBK1 509 S Phosphorylation 18691976 TBK1 584 K Acetylation 19608861 TBK1 672 T Phosphorylation 18691976 TBK1 716 S Phosphorylation 18691976

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9UHD2 172 S 11839743 LTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9UHD2 172 Phosphoserine; by autocatalysis and IKKB 11839743;23746807 Q9UHD2 716 Phosphoserine 23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type TBK1 IPI00293613 S 172 DDEQFVpSLYGTEE phosphorylation TBK1 IPI00293613 S 716 ILERFGpSLTMDGG phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 29110 118878 - K Ubiquitination 18636086 29110 118878 - K Ubiquitination 21987572 29110 118878 - K Ubiquitination 21139048 29110 118878 - K Ubiquitination 21906983 29110 118878 - K Ubiquitination 22388039 29110 118878 - K Ubiquitination 21885437 29110 118878 - K Ubiquitination 22945920 29110 118878 - K Ubiquitination 23028469 29110 118878 - K Ubiquitination 22505724 29110 118878 - K Ubiquitination 23308279 29110 118878 - K Ubiquitination 21963094 29110 118878 - K Ubiquitination 23453972 29110 118878 - K Ubiquitination 24622840 29110 118878 - K Ubiquitination 25142606 29110 118878 - K Sumoylation 25409927 29110 118878 - K Sumoylation 21212807

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID TBK1 Homo sapiens (Human) 137; 154; 504; 396; 584; 661; 241; 344 "137,IVHRDIKPGNIMR; 154,DGQSVYKLTDFGA; 504,ISDIHTKLLRLSS; 396,IGLIYEKISLPKV; 584,NEEQIHKFDKQKL; 661,QETLPQKMFTASS; 241,YKIITGKPSGAIS; 344,LVYKQTKIISSNQ" 137:21906983; 154:21906983; 504:21906983; 396:21906983; 584:21906983; 661:21906983; 241:21906983; 344:21906983