PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus NHLRC1 PPP1R3C 18029386,18070875 biogrid exp HSP90AB1 NHLRC1 25036637,22939624 intact exp HSPA4 NHLRC1 19892702 biogrid exp GYS1 NHLRC1 16115820,22578008 biogrid exp NHLRC1 PRDM8 22961547 biogrid exp DVL2 NHLRC1 22223637 biogrid exp UBA1 NHLRC1 15930137 intact exp NHLRC1 NUDC 25036637 intact exp HSPA8 NHLRC1 25036637 intact exp NHLRC1 NUDCD3 25036637 intact exp NNAT NHLRC1 21742036 biogrid exp HSP90AA1 NHLRC1 22939624 biogrid exp UBE2D3 NHLRC1 22223637,15930137, 23023127 biogrid,intact exp, pred UBE2E1 NHLRC1 15930137, 23023127 biogrid,intact exp, pred NHLRC1 STUB1 19892702,21652633, 23023127 biogrid exp, pred AGL NHLRC1 17908927, 21836163 hprd,biogrid exp, pred UBE2H NHLRC1 15930137, 23023127 biogrid,intact exp, pred EPM2A NHLRC1 18029386,16115820,23922729,18617530,22578008,21505799,15930137, 21836163 biogrid,intact exp, pred UBE2D1 NHLRC1 15930137, 23023127 biogrid,intact exp, pred BIRC2 NHLRC1 23023127 pred BRCA1 NHLRC1 23023127 pred UBE2N NHLRC1 25402006, 23023127 pred NHLRC1 TRAF6 23023127 pred CDC34 NHLRC1 23023127 pred BMI1 NHLRC1 23023127 pred UBE2G2 NHLRC1 23023127 pred ARIH2 NHLRC1 23023127 pred PSMD10 NHLRC1 23023127 pred NHLRC1 AMFR 23023127 pred UBE2B NHLRC1 23023127 pred UBE2L3 NHLRC1 23023127 pred RNF103 NHLRC1 23023127 pred UBE2D2 NHLRC1 23023127 pred UBE2K NHLRC1 23023127 pred NHLRC1 CHFR 23023127 pred NHLRC1 TRIM17 23023127 pred TRIM21 NHLRC1 23023127 pred UBE2V2 NHLRC1 23023127 pred RNF4 NHLRC1 23023127 pred UBE2C NHLRC1 23023127 pred UBE2A NHLRC1 23023127 pred NHLRC1 WDSUB1 23023127 pred NHLRC1 PRPF19 23023127 pred NHLRC1 UBE2E3 23023127 pred NHLRC1 DZIP3 23023127 pred NHLRC1 RNF135 23023127 pred NHLRC1 RNF31 23023127 pred ERCC8 NHLRC1 23023127 pred UBE2V1 NHLRC1 23023127 pred UBE4A NHLRC1 23023127 pred NHLRC1 SMURF1 23023127 pred TTC3 NHLRC1 23023127 pred NHLRC1 RNF41 23023127 pred NHLRC1 RLIM 23023127 pred UBE2M NHLRC1 23023127 pred NHLRC1 RNF167 23023127 pred UBOX5 NHLRC1 23023127 pred LRSAM1 NHLRC1 23023127 pred NHLRC1 HUWE1 23023127 pred UBE2S NHLRC1 23023127 pred RNF8 NHLRC1 23023127 pred NHLRC1 UBE2D4 23023127 pred NHLRC1 UBE2F 23023127 pred

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q9UNE7 Q6VVB1 affinity chromatography technology 19892702 physical association X 2 Q9UNE7 Q6VVB1 affinity chromatography technology 21652633 physical association X 2 P34932 Q6VVB1 affinity chromatography technology 19892702 physical association X 2 O14641 Q6VVB1 two hybrid 22223637 direct interaction X 2 O14641 Q6VVB1 affinity chromatography technology 22223637 physical association X 2 O14641 Q6VVB1 enzymatic study 22223637 direct interaction X 2 O14641 Q6VVB1 imaging technique 22223637 colocalization X 2 Q6VVB1 O95278-2 affinity chromatography technology 18617530 physical association X 2 Q6VVB1 O95278-2 affinity chromatography technology 18617530 physical association X 2 Q6VVB1 O95278-2 two hybrid 21505799 direct interaction X 2 Q6VVB1 O95278-2 affinity chromatography technology 22578008 physical association X 2 Q6VVB1 A0A024RDC4 affinity chromatography technology 22961547 physical association X 2 Q6VVB1 A0A024RDC4 affinity chromatography technology 22961547 physical association X 2 Q6VVB1 P62837 pull down 15930137 direct interaction X 2 Q6VVB1 P61077-3 pull down 22223637 direct interaction X 2 Q6VVB1 O95278-2 two hybrid 23922729 direct interaction X 2 Q6VVB1 O95278-2 affinity chromatography technology 23922729 physical association X 2 Q6VVB1 P61077-3 pull down 15930137 direct interaction X 2 Q6VVB1 A0A024R2K3 pull down 15930137 direct interaction X 2 Q6VVB1 O95278-2 pull down 22578008 direct interaction X 2 Q6VVB1 P13807-2 pull down 22578008 direct interaction X 2 Q6VVB1 P35573-2 affinity chromatography technology 17908927 physical association X 2 Q6VVB1 O95278-2 imaging technique 17337485 colocalization X 2 Q6VVB1 O95278-2 two hybrid 16115820 direct interaction X 2 Q6VVB1 O95278-2 affinity chromatography technology 16115820 physical association X 2 Q6VVB1 O95278-2 affinity chromatography technology 16115820 physical association X 2 Q6VVB1 P13807-2 affinity chromatography technology 16115820 physical association X 2 Q6VVB1 O95278-2 pull down 15930137 direct interaction X 2 Q6VVB1 O95278-2 two hybrid 15930137 direct interaction X 2 Q6VVB1 O95278-2 affinity chromatography technology 15930137 physical association X 2 Q6VVB1 O95278-2 affinity chromatography technology 15930137 physical association X 2 Q6VVB1 O95278-2 enzymatic study 15930137 direct interaction X 2 Q6VVB1 O95278-2 two hybrid 18029386 direct interaction X 2 P11021 Q6VVB1 imaging technique 17337485 colocalization X 2 Q16517 Q6VVB1 two hybrid 21742036 direct interaction X 2 Q16517 Q6VVB1 affinity chromatography technology 21742036 physical association X 2 Q9NRH3 Q6VVB1 imaging technique 17337485 colocalization X 2 A0A087WW00 Q6VVB1 pull down 15930137 direct interaction X 2 A0A087WW00 Q6VVB1 imaging technique 17337485 colocalization X 2 Q6VVB1 P07900-2 affinity chromatography technology 22939624 physical association X 2 Q6VVB1 pull down 15930137 direct interaction X 2 P0CG48 Q6VVB1 affinity chromatography technology 22578008 physical association X 2 P0CG48 Q6VVB1 imaging technique 17337485 colocalization X 2 P0CG48 Q6VVB1 affinity chromatography technology 16115820 physical association X 2 Q9UQK1 Q6VVB1 enzymatic study 18070875 direct interaction X 2 Q9UQK1 Q6VVB1 two hybrid 18029386 direct interaction X 2 Q6VVB1 P35573-2 in vivo 17908927 - X 2 Q6VVB1 P35573-2 in vivo 17908927 - X 2 Q6VVB1 A0A024RD80 luminescence based mammalian interactome mapping 22939624 physical association X 2 Q6VVB1 Q96BE0 luminescence based mammalian interactome mapping 25036637 physical association X 2 O95278 Q6VVB1 two hybrid 15930137 physical association X 2 O95278 Q6VVB1 two hybrid 15930137 physical association X 2 O95278 Q6VVB1 pull down 15930137 physical association X 2 O95278 Q6VVB1 anti tag coimmunoprecipitation 15930137 physical association X 2 O95278 Q6VVB1 confocal microscopy 21505799 colocalization X 2 O95278 Q6VVB1 two hybrid 21505799 physical association X 2 Q6VVB1 ubiquitinase assay 15930137 physical association C 3 Q6VVB1 ubiquitinase assay 15930137 physical association C 3 Q6VVB1 ubiquitinase assay 15930137 physical association C 3 Q6VVB1 affinity chromatography technology 22578008 association C 3 Q6VVB1 ubiquitinase assay 15930137 physical association C 3 Q6VVB1 ubiquitinase assay 15930137 physical association C 3 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q9WUA5 anti tag coimmunoprecipitation 22578008 physical association X 2 Q6VVB1 Q6PK50 luminescence based mammalian interactome mapping 25036637 physical association X 2 Q8IVD9 Q6VVB1 luminescence based mammalian interactome mapping 25036637 physical association X 2 Q9Y266 Q6VVB1 luminescence based mammalian interactome mapping 25036637 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B UBE2D2 NHLRC1 MI:0096(pull down) 15930137 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_225738 prey bait HSPA5 NHLRC1 MI:0428(imaging technique) 17337485 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization) (biogrid) BIOGRID_119087 prey bait DVL2 NHLRC1 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 22223637 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0403(colocalization) (biogrid)|(biogrid)|(biogrid)|(biogrid) BIOGRID_162665|BIOGRID_162664|BIOGRID_162667|BIOGRID_162668 bait|prey|prey|prey prey|bait|bait|bait HSP90AA1 NHLRC1 MI:0004(affinity chromatography technology) 22939624 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_255420 bait prey UBC NHLRC1 MI:0018(two hybrid)|MI:0004(affinity chromatography technology)|MI:0428(imaging technique) 16115820,17337485,22578008 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0403(colocalization) (biogrid)|(biogrid)|(biogrid) BIOGRID_126533|BIOGRID_199862|BIOGRID_119086 prey|prey|prey bait|bait|bait NHLRC1 TUBG2 MI:0428(imaging technique) 17337485 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization) (biogrid) BIOGRID_119085 bait prey NHLRC1 PRDM8 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 22961547 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid) BIOGRID_218143|BIOGRID_218142 prey|bait bait|prey GYS1 NHLRC1 MI:0018(two hybrid) 16115820 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_126534 bait prey HSP90AB1 NHLRC1 MI:0729(luminescence based mammalian interactome mapping) 22939624 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (intact) UniProt_125056 prey bait AGL NHLRC1 MI:0493(in vivo)|MI:0004(affinity chromatography technology) 17908927 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) -|MI:0915(physical association) MI:0468(hprd)|(biogrid) HPRD_33450|BIOGRID_117863 -|prey -|bait NHLRC1 PPP1R3C MI:0415(enzymatic study)|MI:0018(two hybrid) 18029386,18070875 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0407(direct interaction) (biogrid)|(biogrid) BIOGRID_140058|BIOGRID_148010 bait|prey prey|bait NHLRC1 STUB1 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology) 19892702,21652633 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid) BIOGRID_193862|BIOGRID_193911 prey|prey bait|bait NNAT NHLRC1 MI:0018(two hybrid)|MI:0018(two hybrid) 21742036 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction) (biogrid)|(biogrid) BIOGRID_164807|BIOGRID_164806 bait|prey prey|bait UBE2D1 NHLRC1 MI:0428(imaging technique) 17337485 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization) (biogrid) BIOGRID_119088 prey bait UBE2D3 NHLRC1 MI:0018(two hybrid) 22223637 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_226158 prey bait HSPA4 NHLRC1 MI:0004(affinity chromatography technology) 19892702 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_193864 prey bait EPM2A NHLRC1 MI:0096(pull down)|MI:0096(pull down)|MI:0018(two hybrid)|MI:0004(affinity chromatography technology)|MI:0096(pull down)|MI:0004(affinity chromatography technology)|MI:0428(imaging technique)|MI:0096(pull down)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0096(pull down)|MI:0096(pull down)|MI:0018(two hybrid)|MI:0096(pull down) 15930137,16115820,17337485,18029386,18617530,21505799,22578008,23922729 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0403(colocalization)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association) (biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid) BIOGRID_127737|BIOGRID_127738|BIOGRID_148989|BIOGRID_199864|BIOGRID_127742|BIOGRID_199863|BIOGRID_119084|BIOGRID_127739|BIOGRID_196955|BIOGRID_196956|BIOGRID_126528|BIOGRID_198932|BIOGRID_126527|BIOGRID_284771|BIOGRID_284772|BIOGRID_126529|BIOGRID_127740 bait|prey|bait|bait|prey|prey|prey|prey|bait|prey|bait|bait|bait|bait|bait|prey|bait prey|bait|prey|prey|bait|bait|bait|bait|prey|bait|prey|prey|prey|prey|prey|bait|prey

PPI - HINT Gene A Gene B PMID Method Quality EPM2A NHLRC1 15930137,15930137,22578008,22578008,21505799,16115820,21505799,22578008,15930137,23922729,15930137,26648032,17337485,18617530,15930137,23922729,26648032,26546463,16115820,15930137 0096,0997,0096,0004,0663,0018,0018,0415,0007,0004,0415,0018,0428,0004,0018,0018,0096,0004,0004,0004 LC UBC NHLRC1 17337485,22578008,16115820 0428,0004,0004 LC GYS1 NHLRC1 16115820,22578008 0004,0096 LC UBE2D1 NHLRC1 15930137,15930137,17337485 0997,0096,0428 LC UBE2D3 NHLRC1 15930137,15930137,22223637 0997,0096,0096 LC NHLRC1 STUB1 21652633,19892702 0004,0004 LC EPM2A NHLRC1 15930137,15930137,22578008,22578008,21505799,16115820,21505799,22578008,15930137,23922729,15930137,26648032,17337485,18617530,15930137,23922729,26648032,26546463,16115820,15930137 0096,0997,0096,0004,0663,0018,0018,0415,0007,0004,0415,0018,0428,0004,0018,0018,0096,0004,0004,0004 LC UBC NHLRC1 17337485,22578008,16115820 0428,0004,0004 LC GYS1 NHLRC1 16115820,22578008 0004,0096 LC UBE2D1 NHLRC1 15930137,15930137,17337485 0997,0096,0428 LC UBE2D3 NHLRC1 15930137,15930137,22223637 0997,0096,0096 LC NHLRC1 STUB1 21652633,19892702 0004,0004 LC EPM2A NHLRC1 15930137,15930137,22578008,22578008,21505799,16115820,21505799,22578008,15930137,23922729,15930137,26648032,18029386,15930137,23922729,26648032,16115820,15930137 0096,0997,0096,0004,0663,0018,0018,0415,0007,0004,0415,0018,0018,0018,0018,0096,0004,0004 LC DVL2 NHLRC1 22223637,22223637,22223637,22223637 0004,0415,0018,0428 LC HSP90AB1 NHLRC1 25036637,22939624 0729,0729 HT UBA1 NHLRC1 15930137 0997 LC UBE2D1 NHLRC1 15930137,15930137 0997,0096 LC UBE2E1 NHLRC1 15930137,15930137 0997,0096 LC UBE2D3 NHLRC1 15930137,15930137 0997,0096 LC UBE2H NHLRC1 15930137,15930137 0997,0096 LC NNAT NHLRC1 21742036,21742036 0018,0004 LC NHLRC1 NHLRC1 26648032,26648032 0018,0004 LC NHLRC1 NUDCD3 25036637 0729 HT NHLRC1 Q96BE0 25036637 0729 HT NHLRC1 PPP1R3C 18029386,18070875 0018,0415 LC NHLRC1 NUDC 25036637 0729 HT

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q6VVB1 O14641 NHLRC1 DVL2 0.671 22223637 Q6VVB1 P61088 NHLRC1 UBE2N 0.21 26546463 Q9NQV8 Q6VVB1 PRDM8 NHLRC1 0.376 22961547 Q6VVB1 P07900 NHLRC1 HSP90AA1 0.126 22939624 Q6VVB1 P08238 NHLRC1 HSP90AB1 0.126 22939624 Q6VVB1 P13807 NHLRC1 GYS1 0.454 16115820,22578008 Q6VVB1 P34932 NHLRC1 HSPA4 0.21 19892702 Q6VVB1 P35573 NHLRC1 AGL 0.21 17908927 Q6VVB1 P51668 NHLRC1 UBE2D1 0.309 15930137 Q6VVB1 P51965 NHLRC1 UBE2E1 0.309 15930137 Q6VVB1 P62256 NHLRC1 UBE2H 0.309 15930137 Q6VVB1 Q6PK50 NHLRC1 HSP90AB1 0.126 25036637 Q8IVD9 Q6VVB1 NUDCD3 NHLRC1 0.126 25036637 Q96BE0 Q6VVB1 Q96BE0 NHLRC1 0.126 25036637 Q9Y266 Q6VVB1 NUDC NHLRC1 0.126 25036637 Q9Z1E4 Q6VVB1 GYS1 NHLRC1 0.21 22578008 Q9UNE7 Q6VVB1 STUB1 NHLRC1 0.376 21652633,19892702 Q6VVB1 P61077 NHLRC1 UBE2D3 0.523 22223637,15930137 Q6VVB1 Q6VVB1 NHLRC1 NHLRC1 0.49 26648032 Q9WUA5 Q6VVB1 EPM2A NHLRC1 0.376 22578008 Q6VVB1 O95278 NHLRC1 EPM2A 0.997 18029386,15930137,21505799,23922729,18617530,26546463,16115820,22578008,26648032 Q9UQK1 Q6VVB1 PPP1R3C NHLRC1 0.523 18070875,18029386 Q6VVB1 Q16517 NHLRC1 NNAT 0.454 21742036

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 DVL2 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database EPM2A NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database HSP90AA1 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database HSP90AB1 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database UBC NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database HSPA1A NHLRC1 MI:0362 inference MI:0461 interaction database HSPA1B NHLRC1 MI:0362 inference MI:0461 interaction database HSPA8 NHLRC1 MI:0362 inference MI:0461 interaction database GYS1 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database HSPA6 NHLRC1 MI:0362 inference MI:0461 interaction database UBA1 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database HSPA4 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database AGL NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database HTT NHLRC1 MI:0362 inference MI:0461 interaction database UBE2D1 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database UBE2E1 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database HSPA2 NHLRC1 MI:0362 inference MI:0461 interaction database GYS2 NHLRC1 MI:0362 inference MI:0461 interaction database UBE2D3 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database UBE2N NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database UBE2H NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database UBE2D2 NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database NNAT NHLRC1 MI:0045 experimental interaction detection MI:0461 interaction database NHLRC1 NUDCD3 MI:0045 experimental interaction detection MI:0461 interaction database NHLRC1 PRDM8 MI:0045 experimental interaction detection MI:0461 interaction database NHLRC1 STUB1 MI:0045 experimental interaction detection MI:0461 interaction database NHLRC1 PPP1R3C MI:0045 experimental interaction detection MI:0461 interaction database NHLRC1 NUDC MI:0045 experimental interaction detection MI:0461 interaction database