PTM - CPLM Gene Name UniProt ID Type Position Code SUMO2; SMT3B; SMT3H2 P61956 Acetylation 7 K SUMO2; SMT3B; SMT3H2 P61956 Acetylation 11 K SUMO2; SMT3B; SMT3H2 P61956 Sumoylation 7 K SUMO2; SMT3B; SMT3H2 P61956 Sumoylation 11 K SUMO2; SMT3B; SMT3H2 P61956 Sumoylation 5 K SUMO2; SMT3B; SMT3H2 P61956 Sumoylation 42 K SUMO2; SMT3B; SMT3H2 P61956 Ubiquitination 11 K SUMO2; SMT3B; SMT3H2 P61956 Ubiquitination 21 K SUMO2; SMT3B; SMT3H2 P61956 Ubiquitination 7 K SUMO2; SMT3B; SMT3H2 P61956 Ubiquitination 33 K SUMO2; SMT3B; SMT3H2 P61956 Ubiquitination 5 K SUMO2; SMT3B; SMT3H2 P61956 Ubiquitination 35 K SUMO2; SMT3B; SMT3H2 P61956 Ubiquitination 42 K SUMO2; SMT3B; SMT3H2 P61956 Ubiquitination 45 K

PTM - dbPAF Gene Name UniProt ID Position Type Species SUMO2; SMT3B; SMT3H2 P61956 12 T Homo sapiens(Human) SUMO2; SMT3B; SMT3H2 P61956 28 S Homo sapiens(Human) SUMO2; SMT3B; SMT3H2 P61956 38 T Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P61956 K5-ac Acetylation ___MADEkPkEGVkt P61956 K11-ac Acetylation EkPkEGVktENNDHI P61956 K33-ac Acetylation DGsVVQFkIkRHtPL P61956 K42-ac Acetylation kRHtPLSkLMkAYCE P61956 K45-ac Acetylation tPLSkLMkAYCErQG P61956 K33-m1 Methylation DGsVVQFkIkRHtPL P61956 K45-m1 Methylation tPLSkLMkAYCErQG P61956 R50-m1 Methylation LMkAYCErQGLsMRQ P61956 T12-p Phosphorylation kPkEGVktENNDHIN P61956 S28-p Phosphorylation kVAGQDGsVVQFkIk P61956 T38-p Phosphorylation QFkIkRHtPLSkLMk P61956 S54-p Phosphorylation YCErQGLsMRQIRFR P61956 K5-sm Sumoylation ___MADEkPkEGVkt P61956 K7-sm Sumoylation _MADEkPkEGVktEN P61956 K11-sm Sumoylation EkPkEGVktENNDHI P61956 K21-sm Sumoylation NNDHINLkVAGQDGs P61956 K33-sm Sumoylation DGsVVQFkIkRHtPL P61956 K42-sm Sumoylation kRHtPLSkLMkAYCE P61956 K45-sm Sumoylation tPLSkLMkAYCErQG P61956 K5-ub Ubiquitination ___MADEkPkEGVkt P61956 K7-ub Ubiquitination _MADEkPkEGVktEN P61956 K11-ub Ubiquitination EkPkEGVktENNDHI P61956 K21-ub Ubiquitination NNDHINLkVAGQDGs P61956 K33-ub Ubiquitination DGsVVQFkIkRHtPL P61956 K35-ub Ubiquitination sVVQFkIkRHtPLSk P61956 K42-ub Ubiquitination kRHtPLSkLMkAYCE P61956 K45-ub Ubiquitination tPLSkLMkAYCErQG

PTM - dbPTM UniProt ID Position Code Type Resources References P61956 11 K Sumoylation Swiss-Prot 1010711 11451954;18408734;20388717 P61956 11 K Acetylation Swiss-Prot 1010711 19608861 P61956 11 K Ubiquitylation Swiss-Prot 1010711 - P61956 93 G Isopeptide bond Swiss-Prot 1010711 - P61956 11 K Sumoylation Phosphositeplus.1010730 11451954;15175327;16194093;19471022;19721078;20388717;20797634;21098080 P61956 42 K Sumoylation Phosphositeplus.1010730 16194093;21098080 P61956 5 K Sumoylation Phosphositeplus.1010730 16194093;17938407 P61956 7 K Sumoylation Phosphositeplus.1010730 16194093 P61956 11 K Ubiquitylation Phosphositeplus.1010730 21906983 P61956 21 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P61956 33 K Ubiquitylation Phosphositeplus.1010730 18565875;21890473;21906983 P61956 35 K Ubiquitylation Phosphositeplus.1010730 21890473 P61956 42 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P61956 45 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 P61956 11 K Acetylation Phosphositeplus.1010730 17938407;19608861 P61956 42 K Acetylation Phosphositeplus.1010730 19608861 P61956 45 K Acetylation Phosphositeplus.1010730 19608861 P61956 28 S Phosphorylation Phosphositeplus.1010730 20068231 P61956 38 T Phosphorylation Phosphositeplus.1010730 20068231;21406692 P61956 11 K Acetylation HPRD 9.0 19608861 P61956 42 K Acetylation HPRD 9.0 19608861 P61956 45 K Acetylation HPRD 9.0 19608861 P61956 28 S Phosphorylation HPRD 9.0 20068231 P61956 38 T Phosphorylation HPRD 9.0 20068231 P61956 11 K Sumoylation HPRD 9.0 11451954

PTM - HPRD Symbol Position Code Type References SUMO2 11 K Acetylation 19608861 SUMO2 11 K Sumoylation 11451954 SUMO2 28 S Phosphorylation 20068231 SUMO2 38 T Phosphorylation 20068231 SUMO2 42 K Acetylation 19608861 SUMO2 45 K Acetylation 19608861 SUMO2 68 G Proteolytic Cleavage 11489887

PTM - UniProt UniProt ID Position Type PMIDs P61956 11 N6-acetyllysine; alternate 19608861

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type SUMO2 IPI00140827 K 11 KPKEGVaKTENNDH acetylation SUMO2 IPI00140827 K 42 RHTPLSaKLMKAYC acetylation SUMO2 IPI00140827 K 45 PLSKLMaKAYCERQ acetylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 6613 112497 - K Sumoylation 11451954 6613 112497 - K Ubiquitination 22118674 6613 112497 - K Ubiquitination 21987572 6613 112497 - K Sumoylation 21693764 6613 112497 - K Ubiquitination 21139048 6613 112497 - K Ubiquitination 21890473 6613 112497 - K Ubiquitination 21906983 6613 112497 - K Sumoylation 16236267 6613 112497 - K Sumoylation 19471022 6613 112497 - K Sumoylation 21252943 6613 112497 - K Sumoylation 15175327 6613 112497 - K Ubiquitination 22505724 6613 112497 - K FAT10ylation 22797925 6613 112497 - K Ubiquitination 21963094 6613 112497 - K Ubiquitination 22053931 6613 112497 - K Ubiquitination 23000965 6613 112497 - K Sumoylation 24151981 6613 112497 - K Ubiquitination 24816145 6613 112497 - K Sumoylation 25027693 6613 112497 - K Sumoylation 24969970 6613 112497 - K Ubiquitination 25015289 6613 112497 - K Ubiquitination 25147182 6613 112497 - K Sumoylation 25391492 6613 112497 - K Sumoylation 20029837 6613 112497 - K Sumoylation 17938407 6613 112497 - K Sumoylation 21098080

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID SUMO2 Homo sapiens (Human) 34; 32; 20; 41; 44 "34,VVQFKIKRHTPLS; 32,GSVVQFKIKRHTP; 20,NDHINLKVAGQDG; 41,RHTPLSKLMKAYC; 44,PLSKLMKAYCERQ" "34:21890473; 32:21906983,21890473; 20:21906983; 41:21906983,21890473; 44:21906983,21890473" SUMO2 Homo sapiens (Human) 35; 33; 21; 42; 45 "35,VVQFKIKRHTPLS; 33,GSVVQFKIKRHTP; 21,NDHINLKVAGQDG; 42,RHTPLSKLMKAYC; 45,PLSKLMKAYCERQ" 35:21890473; 33:21890473; 21:21890473; 42:21890473; 45:21890473 SUMO2 Homo sapiens (Human) 33; 21; 42; 11; 45 "33,GSVVQFKIKRHTP; 21,NDHINLKVAGQDG; 42,RHTPLSKLMKAYC; 11,KPKEGVKTENNDH; 45,PLSKLMKAYCERQ" 33:21906983; 21:21906983; 42:21906983; 11:21906983; 45:21906983 SUMO2 Homo sapiens (Human) 35; 33; 21; 5; 7; 42; 11; 45 "35,VVQFKIKRHTPLS; 33,GSVVQFKIKRHTP; 21,NDHINLKVAGQDG; 5,MADEKPKEGVK; 7,MADEKPKEGVKTE; 42,RHTPLSKLMKAYC; 11,KPKEGVKTENNDH; 45,PLSKLMKAYCERQ" "35:21890473; 33:21139048,21890473,21906983; 21:21139048,21890473,21906983; 5:21139048,21890473,21906983; 7:21139048; 42:21890473,21906983; 11:21139048,21906983; 45:21890473,21906983"