PTM - CPLM Gene Name UniProt ID Type Position Code WDR5; BIG3 P61964 Acetylation 112 K WDR5; BIG3 P61964 Ubiquitination 27 K WDR5; BIG3 P61964 Ubiquitination 32 K WDR5; BIG3 P61964 Ubiquitination 227 K WDR5; BIG3 P61964 Ubiquitination 120 K WDR5; BIG3 P61964 Ubiquitination 159 K WDR5; BIG3 P61964 Ubiquitination 7 K WDR5; BIG3 P61964 Ubiquitination 6 K

PTM - dbPAF Gene Name UniProt ID Position Type Species WDR5; BIG3 P61964 131 Y Homo sapiens(Human) WDR5; BIG3 P61964 18 T Homo sapiens(Human) WDR5; BIG3 P61964 20 S Homo sapiens(Human) WDR5; BIG3 P61964 22 S Homo sapiens(Human) WDR5; BIG3 P61964 228 Y Homo sapiens(Human) WDR5; BIG3 P61964 267 S Homo sapiens(Human) WDR5; BIG3 P61964 290 T Homo sapiens(Human) WDR5; BIG3 P61964 54 S Homo sapiens(Human) WDR5; BIG3 P61964 62 S Homo sapiens(Human) WDR5; BIG3 P61964 63 S Homo sapiens(Human) WDR5; BIG3 P61964 64 S Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P61964 K112-ac Acetylation ASDDKTLkIWDVSSG P61964 K38-m1 Methylation VkPNYALkFTLAGHT P61964 T18-p Phosphorylation EAARAQPtPSSSATQ P61964 T29-p Phosphorylation SATQSkPtPVkPNYA P61964 S54-p Phosphorylation AVSSVKFsPNGEWLA P61964 Y131-p Phosphorylation TLKGHSNyVFCCNFN P61964 Y228-p Phosphorylation KFSPNGkyILAATLD P61964 S267-p Phosphorylation YCIFANFsVTGGKWI P61964 T290-p Phosphorylation VYIWNLQtKEIVQKL P61964 K46-sm Sumoylation FTLAGHTkAVSSVKF P61964 K27-ub Ubiquitination SSSATQSkPtPVkPN P61964 K32-ub Ubiquitination QSkPtPVkPNYALkF P61964 K120-ub Ubiquitination IWDVSSGkCLKTLKG P61964 K159-ub Ubiquitination SVRIWDVkTGKCLKT P61964 K227-ub Ubiquitination VKFSPNGkyILAATL

PTM - dbPTM UniProt ID Position Code Type Resources References P61964 112 K Acetylation Swiss-Prot 1010711 19608861 P61964 267 S Phosphorylation Swiss-Prot 1010711 17924679 P61964 159 K Ubiquitylation Phosphositeplus.1010730 21906983 P61964 227 K Ubiquitylation Phosphositeplus.1010730 21890473 P61964 27 K Ubiquitylation Phosphositeplus.1010730 21890473 P61964 32 K Ubiquitylation Phosphositeplus.1010730 21890473 P61964 112 K Acetylation Phosphositeplus.1010730 19608861 P61964 112 K Acetylation HPRD 9.0 19608861 P61964 267 S Phosphorylation HPRD 9.0 17924679

PTM - HPRD Symbol Position Code Type References WDR5 112 K Acetylation 19608861 WDR5 267 S Phosphorylation 17924679

PTM - UniProt UniProt ID Position Type PMIDs P61964 2 N-acetylalanine 22814378 P61964 112 N6-acetyllysine 19608861

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type WDR5 IPI00005492 K 112 SDDKTLaKIWDVSS acetylation WDR5 IPI00005492 S 54 VSSVKFpSPNGEWL phosphorylation WDR5 IPI00005492 S 62 NGEWLApSSSADKL phosphorylation WDR5 IPI00005492 S 63 GEWLASpSSADKLI phosphorylation WDR5 IPI00005492 S 64 EWLASSpSADKLIK phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 11091 116272 - K Ubiquitination 21139048 11091 116272 - K Ubiquitination 21890473 11091 116272 - K Ubiquitination 21906983 11091 116272 - K Sumoylation 16236267 11091 116272 - K Sumoylation 19471022 11091 116272 - K Neddylation 18247557 11091 116272 - K Ubiquitination 23000965 11091 116272 - K Ubiquitination 24816145 11091 116272 - K Sumoylation 25391492

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID WDR5 Homo sapiens (Human) 32; 159; 27; 227 "32,SKPTPVKPNYALK; 159,VRIWDVKTGKCLK; 27,SSATQSKPTPVKP; 227,KFSPNGKYILAAT" 32:21890473; 159:21906983; 27:21890473; 227:21890473