PTM - CPLM Gene Name UniProt ID Type Position Code Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Acetylation 671 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Acetylation 528 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Acetylation 838 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Acetylation 802 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Succinylation 528 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 627 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 635 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 746 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 889 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 296 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 299 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 304 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 322 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 443 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 465 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 526 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 528 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 657 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 671 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 802 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 806 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 838 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 843 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 882 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 374 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 593 K Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 Ubiquitination 604 K

PTM - dbPAF Gene Name UniProt ID Position Type Species Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 13 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 131 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 21 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 24 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 28 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 281 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 3 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 31 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 377 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 4 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 46 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 55 Y Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 670 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 793 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 800 T Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 803 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 810 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 816 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 820 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 832 T Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 835 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 876 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 883 S Mus musculus(Mouse) Uba1; Sbx; Ube1; Ube1ax; Ube1x Q02053 971 T Mus musculus(Mouse)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q02053 K465-ac Acetylation FGSDFQEkLSKQKYF Q02053 K671-ac Acetylation NQYLTDskFVERTLR Q02053 S4-p Phosphorylation ____MSSsPLSKKRR Q02053 S13-p Phosphorylation LSKKRRVsGPDPKPG Q02053 S21-p Phosphorylation GPDPKPGsNCsPAQs Q02053 S24-p Phosphorylation PKPGsNCsPAQsALs Q02053 S28-p Phosphorylation sNCsPAQsALsEVSS Q02053 S31-p Phosphorylation sPAQsALsEVSSVPT Q02053 S46-p Phosphorylation NGMAKNGsEADIDES Q02053 Y55-p Phosphorylation ADIDESLySRQLyVL Q02053 Y60-p Phosphorylation SLySRQLyVLGHEAM Q02053 S131-p Phosphorylation GKNRAEVsQPRLAEL Q02053 S281-p Phosphorylation TFSICDTsNFSDYIR Q02053 S377-p Phosphorylation PPSVkQNsLDEDLIR Q02053 Y388-p Phosphorylation DLIRkLAyVAAGDLA Q02053 T531-p Phosphorylation VTkLkSDtAAAAVRQ Q02053 Y542-p Phosphorylation AVRQMNPyIQVTSHQ Q02053 S670-p Phosphorylation VNQYLTDskFVERTL Q02053 S793-p Phosphorylation AVASLLQsVQVPEFt Q02053 T800-p Phosphorylation sVQVPEFtPkSGVkI Q02053 S810-p Phosphorylation SGVkIHVsDQELQsA Q02053 S816-p Phosphorylation VsDQELQsANAsVDD Q02053 S820-p Phosphorylation ELQsANAsVDDSRLE Q02053 T832-p Phosphorylation RLEELKAtLPsPDkL Q02053 S835-p Phosphorylation ELKAtLPsPDkLPGF Q02053 S876-p Phosphorylation RAENYDIsPADRHks Q02053 S883-p Phosphorylation sPADRHksKLIAGkI Q02053 T971-p Phosphorylation QPNGEEMtLKQFLDY Q02053 S1044-p Phosphorylation ELCCNDEsGEDVEVP Q02053 K185-ub Ubiquitination FCHSRGIkLVVADTR Q02053 K296-ub Ubiquitination GGIVSQVkVPkKISF Q02053 K299-ub Ubiquitination VSQVkVPkKISFkSL Q02053 K304-ub Ubiquitination VPkKISFkSLPASLV Q02053 K322-ub Ubiquitination FVMTDFAkYSRPAQL Q02053 K374-ub Ubiquitination ARSPPSVkQNsLDED Q02053 K385-ub Ubiquitination LDEDLIRkLAyVAAG Q02053 K443-ub Ubiquitination KEALTEEkCLPRQNR Q02053 K465-ub Ubiquitination FGSDFQEkLSKQKYF Q02053 K526-ub Ubiquitination FRPWDVTkLkSDtAA Q02053 K528-ub Ubiquitination PWDVTkLkSDtAAAA Q02053 K593-ub Ubiquitination RRCVYYRkPLLESGT Q02053 K604-ub Ubiquitination ESGTLGTkGNVQVVI Q02053 K627-ub Ubiquitination SSQDPPEkSIPICTL Q02053 K635-ub Ubiquitination SIPICTLkNFPNAIE Q02053 K657-ub Ubiquitination DEFEGLFkQPAENVN Q02053 K671-ub Ubiquitination NQYLTDskFVERTLR Q02053 K746-ub Ubiquitination APFWSGPkRCPHPLT Q02053 K802-ub Ubiquitination QVPEFtPkSGVkIHV Q02053 K806-ub Ubiquitination FtPkSGVkIHVsDQE Q02053 K838-ub Ubiquitination AtLPsPDkLPGFkMY Q02053 K843-ub Ubiquitination PDkLPGFkMYPIDFE Q02053 K851-ub Ubiquitination MYPIDFEkDDDSNFH Q02053 K882-ub Ubiquitination IsPADRHksKLIAGk Q02053 K889-ub Ubiquitination ksKLIAGkIIPAIAT Q02053 K923-ub Ubiquitination HQQLDSYkNGFLNLA

PTM - dbPTM UniProt ID Position Code Type Resources References Q02053 21 S Phosphorylation Swiss-Prot 1010711 17242355 Q02053 24 S Phosphorylation Swiss-Prot 1010711 17242355 Q02053 31 S Phosphorylation Swiss-Prot 1010711 17242355 Q02053 46 S Phosphorylation Swiss-Prot 1010711 17203969 Q02053 55 Y Phosphorylation Swiss-Prot 1010711 17947660 Q02053 816 S Phosphorylation Swiss-Prot 1010711 17242355 Q02053 820 S Phosphorylation Swiss-Prot 1010711 17242355 Q02053 835 S Phosphorylation Swiss-Prot 1010711 17242355 Q02053 21 S Phosphorylation PhosphoELM.10011 17242355 Q02053 24 S Phosphorylation PhosphoELM.10011 17242355 Q02053 31 S Phosphorylation PhosphoELM.10011 17242355 Q02053 816 S Phosphorylation PhosphoELM.10011 17242355 Q02053 820 S Phosphorylation PhosphoELM.10011 17242355 Q02053 835 S Phosphorylation PhosphoELM.10011 17242355 Q02053 13 S Phosphorylation Phosphositeplus.1010730 19854140;21183079;21659605 Q02053 21 S Phosphorylation Phosphositeplus.1010730 21183079;21659605 Q02053 24 S Phosphorylation Phosphositeplus.1010730 21183079;21659605 Q02053 28 S Phosphorylation Phosphositeplus.1010730 21183079;21659605 Q02053 31 S Phosphorylation Phosphositeplus.1010730 21659605 Q02053 46 S Phosphorylation Phosphositeplus.1010730 17203969;18846507;19367708;19854140;20531401;21183079;21659604;21659605 Q02053 670 S Phosphorylation Phosphositeplus.1010730 18779572 Q02053 810 S Phosphorylation Phosphositeplus.1010730 20415495;21183079;21659605 Q02053 816 S Phosphorylation Phosphositeplus.1010730 17242355;21183079;21659604 Q02053 820 S Phosphorylation Phosphositeplus.1010730 17242355;21183079;21659604 Q02053 835 S Phosphorylation Phosphositeplus.1010730 18846507;19737024;19854140;20415495;20531401;21183079 Q02053 876 S Phosphorylation Phosphositeplus.1010730 20415495 Q02053 800 T Phosphorylation Phosphositeplus.1010730 20415495 Q02053 55 Y Phosphorylation Phosphositeplus.1010730 17947660;18515860 Q02053 234 C S-nitrosylation dbSNO 1.0 21278135 Q02053 41 M Oxidation SysPTM 1.1 17242355 Q02053 21 S Phosphorylation SysPTM 1.1 17242355 Q02053 24 S Phosphorylation SysPTM 1.1 17242355 Q02053 31 S Phosphorylation SysPTM 1.1 17242355 Q02053 46 S Phosphorylation SysPTM 1.1 18846507;17203969 Q02053 670 S Phosphorylation SysPTM 1.1 18779572 Q02053 816 S Phosphorylation SysPTM 1.1 17242355 Q02053 820 S Phosphorylation SysPTM 1.1 17242355 Q02053 835 S Phosphorylation SysPTM 1.1 18846507;17242355 Q02053 55 Y Phosphorylation SysPTM 1.1 18515860

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q02053 816 S 17242355 HTP Phosphorylation Q02053 21 S 17242355 HTP Phosphorylation Q02053 820 S 17242355 HTP Phosphorylation Q02053 835 S 17242355 HTP Phosphorylation Q02053 31 S 17242355 HTP Phosphorylation Q02053 24 S 17242355 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q02053 13 Phosphoserine 21183079 Q02053 21 Phosphoserine 21183079 Q02053 24 Phosphoserine 21183079 Q02053 46 Phosphoserine 21183079 Q02053 55 Phosphotyrosine 17947660 Q02053 528 N6-succinyllysine 23806337 Q02053 671 N6-acetyllysine 23806337 Q02053 810 Phosphoserine 21183079 Q02053 816 Phosphoserine 17242355;21183079 Q02053 820 Phosphoserine 17242355;21183079 Q02053 835 Phosphoserine 21183079

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type Uba1 IPI00123313 S 46 GMAKNGpSEADIDE phosphorylation Uba1 IPI00123313 S 810 GVKIHVpSDQELQS phosphorylation Uba1 IPI00123313 S 816 SDQELQpSANASVD phosphorylation Uba1 IPI00123313 S 835 LKATLPpSPDKLPG phosphorylation Uba1 IPI00123313 S 876 AENYDIpSPADRHK phosphorylation Uba1 IPI00123313 T 800 VQVPEFpTPKSGVK phosphorylation

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID Uba1 Mus musculus (Mouse) 299; 802; 806; 882; 838; 843; 657; 671; 443; 296; 627; 635; 304; 322; 528; 526; 746; 374; 465; 851; 593; 604; 185 "299,SQVKVPKKISFKS; 802,VPEFTPKSGVKIH; 806,TPKSGVKIHVSDQ; 882,SPADRHKSKLIAG; 838,TLPSPDKLPGFKM; 843,DKLPGFKMYPIDF; 657,EFEGLFKQPAENV; 671,QYLTDSKFVERTL; 443,EALTEEKCLPRQN; 296,GIVSQVKVPKKIS; 627,SQDPPEKSIPICT; 635,IPICTLKNFPNAI; 304,PKKISFKSLPASL; 322,VMTDFAKYSRPAQ; 528,WDVTKLKSDTAAA; 526,RPWDVTKLKSDTA; 746,PFWSGPKRCPHPL; 374,RSPPSVKQNSLDE; 465,GSDFQEKLSKQKY; 851,YPIDFEKDDDSNF; 593,RCVYYRKPLLESG; 604,SGTLGTKGNVQVV; 185,CHSRGIKLVVADT" 299:22790023; 802:22790023; 806:22790023; 882:22790023; 838:22790023; 843:22790023; 657:22790023; 671:22790023; 443:22790023; 296:22790023; 627:22790023; 635:22790023; 304:22790023; 322:22790023; 528:22790023; 526:22790023; 746:22790023; 374:22790023; 465:22790023; 851:22790023; 593:22790023; 604:22790023; 185:22790023