PTM - CPLM Gene Name UniProt ID Type Position Code Vcp Q01853 Acetylation 231 K Vcp Q01853 Acetylation 529 K Vcp Q01853 Acetylation 505 K Vcp Q01853 Acetylation 668 K Vcp Q01853 Acetylation 651 K Vcp Q01853 Acetylation 512 K Vcp Q01853 Acetylation 502 K Vcp Q01853 Acetylation 251 K Vcp Q01853 Acetylation 754 K Vcp Q01853 Acetylation 658 K Vcp Q01853 Acetylation 565 K Vcp Q01853 Acetylation 60 K Vcp Q01853 Acetylation 336 K Vcp Q01853 Acetylation 217 K Vcp Q01853 Acetylation 109 K Vcp Q01853 Acetylation 148 K Vcp Q01853 Succinylation 60 K Vcp Q01853 Succinylation 148 K Vcp Q01853 Succinylation 668 K Vcp Q01853 Ubiquitination 18 K Vcp Q01853 Ubiquitination 45 K Vcp Q01853 Ubiquitination 60 K Vcp Q01853 Ubiquitination 109 K Vcp Q01853 Ubiquitination 148 K Vcp Q01853 Ubiquitination 211 K Vcp Q01853 Ubiquitination 217 K Vcp Q01853 Ubiquitination 231 K Vcp Q01853 Ubiquitination 236 K Vcp Q01853 Ubiquitination 251 K Vcp Q01853 Ubiquitination 288 K Vcp Q01853 Ubiquitination 295 K Vcp Q01853 Ubiquitination 312 K Vcp Q01853 Ubiquitination 336 K Vcp Q01853 Ubiquitination 386 K Vcp Q01853 Ubiquitination 486 K Vcp Q01853 Ubiquitination 502 K Vcp Q01853 Ubiquitination 505 K Vcp Q01853 Ubiquitination 512 K Vcp Q01853 Ubiquitination 524 K Vcp Q01853 Ubiquitination 529 K Vcp Q01853 Ubiquitination 584 K Vcp Q01853 Ubiquitination 614 K Vcp Q01853 Ubiquitination 615 K Vcp Q01853 Ubiquitination 651 K Vcp Q01853 Ubiquitination 658 K Vcp Q01853 Ubiquitination 668 K Vcp Q01853 Ubiquitination 696 K Vcp Q01853 Ubiquitination 754 K

PTM - dbPAF Gene Name UniProt ID Position Type Species Vcp Q01853 13 S Mus musculus(Mouse) Vcp Q01853 173 Y Mus musculus(Mouse) Vcp Q01853 197 S Mus musculus(Mouse) Vcp Q01853 244 Y Mus musculus(Mouse) Vcp Q01853 282 S Mus musculus(Mouse) Vcp Q01853 284 S Mus musculus(Mouse) Vcp Q01853 3 S Mus musculus(Mouse) Vcp Q01853 352 S Mus musculus(Mouse) Vcp Q01853 37 S Mus musculus(Mouse) Vcp Q01853 42 S Mus musculus(Mouse) Vcp Q01853 509 T Mus musculus(Mouse) Vcp Q01853 644 Y Mus musculus(Mouse) Vcp Q01853 664 S Mus musculus(Mouse) Vcp Q01853 7 S Mus musculus(Mouse) Vcp Q01853 702 S Mus musculus(Mouse) Vcp Q01853 705 S Mus musculus(Mouse) Vcp Q01853 718 S Mus musculus(Mouse) Vcp Q01853 746 S Mus musculus(Mouse) Vcp Q01853 748 S Mus musculus(Mouse) Vcp Q01853 755 Y Mus musculus(Mouse) Vcp Q01853 765 S Mus musculus(Mouse) Vcp Q01853 770 S Mus musculus(Mouse) Vcp Q01853 775 S Mus musculus(Mouse) Vcp Q01853 784 S Mus musculus(Mouse) Vcp Q01853 787 S Mus musculus(Mouse) Vcp Q01853 791 T Mus musculus(Mouse) Vcp Q01853 794 S Mus musculus(Mouse) Vcp Q01853 796 Y Mus musculus(Mouse) Vcp Q01853 805 Y Mus musculus(Mouse)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q01853 K8-ac Acetylation MAsGADskGDDLSTA Q01853 K651-ac Acetylation yIPLPDEkSRVAILk Q01853 K658-ac Acetylation kSRVAILkANLRkSP Q01853 K668-ac Acetylation LRkSPVAkDVDLEFL Q01853 K315-m3 Methylation AIAPkREkTHGEVER Q01853 S3-p Phosphorylation _____MAsGADskGD Q01853 S7-p Phosphorylation _MAsGADskGDDLST Q01853 S37-p Phosphorylation EAINEDNsVVSLsQP Q01853 S42-p Phosphorylation DNsVVSLsQPkMDEL Q01853 S101-p Phosphorylation VRLGDVIsIQPCPDV Q01853 Y173-p Phosphorylation VETDPSPyCIVAPDT Q01853 S197-p Phosphorylation KREDEEEsLNEVGYD Q01853 Y244-p Phosphorylation PPRGILLyGPPGTGk Q01853 S282-p Phosphorylation MSkLAGEsEsNLRkA Q01853 S284-p Phosphorylation kLAGEsEsNLRkAFE Q01853 S352-p Phosphorylation AATNRPNsIDPALRR Q01853 T509-p Phosphorylation kFLkFGMtPSkGVLF Q01853 Y644-p Phosphorylation GRLDQLIyIPLPDEk Q01853 S702-p Phosphorylation CkLAIREsIEsEIRR Q01853 S705-p Phosphorylation AIREsIEsEIRRERE Q01853 S718-p Phosphorylation RERQTNPsAMEVEED Q01853 S746-p Phosphorylation AMRFARRsVsDNDIR Q01853 S748-p Phosphorylation RFARRsVsDNDIRky Q01853 Y755-p Phosphorylation sDNDIRkyEMFAQTL Q01853 S765-p Phosphorylation FAQTLQQsRGFGsFR Q01853 S770-p Phosphorylation QQsRGFGsFRFPsGN Q01853 S775-p Phosphorylation FGsFRFPsGNQGGAG Q01853 S787-p Phosphorylation GAGPSQGsGGGTGGS Q01853 Y796-p Phosphorylation GGTGGSVyTEDNDDD Q01853 Y805-p Phosphorylation EDNDDDLyG______ Q01853 K18-ub Ubiquitination DLSTAILkQKNRPNR Q01853 K45-ub Ubiquitination VVSLsQPkMDELQLF Q01853 K60-ub Ubiquitination RGDTVLLkGKKRREA Q01853 K109-ub Ubiquitination IQPCPDVkYGkRIHV Q01853 K112-ub Ubiquitination CPDVkYGkRIHVLPI Q01853 K148-ub Ubiquitination EAYRPIRkGDIFLVR Q01853 K211-ub Ubiquitination DDIGGCRkQLAQIkE Q01853 K217-ub Ubiquitination RkQLAQIkEMVELPL Q01853 K231-ub Ubiquitination LRHPALFkAIGVkPP Q01853 K236-ub Ubiquitination LFkAIGVkPPRGILL Q01853 K251-ub Ubiquitination yGPPGTGkTLIARAV Q01853 K277-ub Ubiquitination NGPEIMSkLAGEsEs Q01853 K288-ub Ubiquitination EsEsNLRkAFEEAEk Q01853 K295-ub Ubiquitination kAFEEAEkNAPAIIF Q01853 K312-ub Ubiquitination ELDAIAPkREkTHGE Q01853 K336-ub Ubiquitination LTLMDGLkQRAHVIV Q01853 K386-ub Ubiquitination EILQIHTkNMkLADD Q01853 K389-ub Ubiquitination QIHTkNMkLADDVDL Q01853 K486-ub Ubiquitination IGGLEDVkRELQELV Q01853 K502-ub Ubiquitination YPVEHPDkFLkFGMt Q01853 K505-ub Ubiquitination EHPDkFLkFGMtPSk Q01853 K512-ub Ubiquitination kFGMtPSkGVLFYGP Q01853 K524-ub Ubiquitination YGPPGCGkTLLAkAI Q01853 K529-ub Ubiquitination CGkTLLAkAIANECQ Q01853 K584-ub Ubiquitination DELDSIAkARGGNIG Q01853 K614-ub Ubiquitination EMDGMSTkkNVFIIG Q01853 K615-ub Ubiquitination MDGMSTkkNVFIIGA Q01853 K651-ub Ubiquitination yIPLPDEkSRVAILk Q01853 K658-ub Ubiquitination kSRVAILkANLRkSP Q01853 K663-ub Ubiquitination ILkANLRkSPVAkDV Q01853 K668-ub Ubiquitination LRkSPVAkDVDLEFL Q01853 K696-ub Ubiquitination EICQRACkLAIREsI Q01853 K754-ub Ubiquitination VsDNDIRkyEMFAQT

PTM - dbPTM UniProt ID Position Code Type Resources References Q01853 7 S Phosphorylation Swiss-Prot 1010711 17242355 Q01853 7 S Phosphorylation PhosphoELM.10011 17242355 Q01853 796 Y Phosphorylation PhosphoELM.10011 10364224 Q01853 805 Y Phosphorylation PhosphoELM.10011 15592455 Q01853 8 K Acetylation Phosphositeplus.1010730 19594485 Q01853 197 S Phosphorylation Phosphositeplus.1010730 21659604 Q01853 282 S Phosphorylation Phosphositeplus.1010730 18779572;19854140 Q01853 284 S Phosphorylation Phosphositeplus.1010730 18779572 Q01853 7 S Phosphorylation Phosphositeplus.1010730 17242355;20415495 Q01853 702 S Phosphorylation Phosphositeplus.1010730 19854140;21183079;21659604 Q01853 705 S Phosphorylation Phosphositeplus.1010730 19854140 Q01853 748 S Phosphorylation Phosphositeplus.1010730 20415495;21183079 Q01853 770 S Phosphorylation Phosphositeplus.1010730 19854140;21183079;21659605 Q01853 509 T Phosphorylation Phosphositeplus.1010730 21183079 Q01853 173 Y Phosphorylation Phosphositeplus.1010730 18563927 Q01853 244 Y Phosphorylation Phosphositeplus.1010730 18563927 Q01853 644 Y Phosphorylation Phosphositeplus.1010730 19854140 Q01853 755 Y Phosphorylation Phosphositeplus.1010730 19655815 Q01853 796 Y Phosphorylation Phosphositeplus.1010730 10364224;8157674 Q01853 805 Y Phosphorylation Phosphositeplus.1010730 10364224;15592455;15752563;17496150;8157674 Q01853 105 C S-nitrosylation dbSNO 1.0 20925432;21278135 Q01853 174 C S-nitrosylation dbSNO 1.0 20925432 Q01853 209 C S-nitrosylation dbSNO 1.0 20925432 Q01853 535 C S-nitrosylation dbSNO 1.0 20925432;21278135 Q01853 572 C S-nitrosylation dbSNO 1.0 20925432;21278135 Q01853 691 C S-nitrosylation dbSNO 1.0 20925432;21278135 Q01853 282 S Phosphorylation SysPTM 1.1 18779572 Q01853 284 S Phosphorylation SysPTM 1.1 18779572 Q01853 173 Y Phosphorylation SysPTM 1.1 18563927 Q01853 244 Y Phosphorylation SysPTM 1.1 18563927 Q01853 755 Y Phosphorylation SysPTM 1.1 15879432

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q01853 805 Y 15592455 HTP Phosphorylation Q01853 796 Y 10364224 LTP Phosphorylation Q01853 7 S 17242355 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q01853 315 N6,N6,N6-trimethyllysine; by VCPKMT 22948820 Q01853 502 N6-acetyllysine 23806337 Q01853 505 N6-acetyllysine 23806337 Q01853 668 N6-acetyllysine; alternate 23806337 Q01853 668 N6-succinyllysine; alternate 23806337 Q01853 754 N6-acetyllysine 23806337 Q01853 770 Phosphoserine 21183079 Q01853 805 Phosphotyrosine 15592455

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type Vcp IPI00622235 S 7 MASGADpSKGDDLS phosphorylation Vcp IPI00622235 S 748 FARRSVpSDNDIRK phosphorylation

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID Vcp Mus musculus (Mouse) 18; 60; 45; 231; 696; 295; 312; 236; 251; 502; 505; 512; 486; 386; 524; 529; 336; 288; 148; 615; 211; 217; 668; 754; 651; 109; 584; 658; 614 "18,LSTAILKQKNRPN; 60,GDTVLLKGKKRRE; 45,VSLSQPKMDELQL; 231,RHPALFKAIGVKP; 696,ICQRACKLAIRES; 295,AFEEAEKNAPAII; 312,LDAIAPKREKTHG; 236,FKAIGVKPPRGIL; 251,GPPGTGKTLIARA; 502,PVEHPDKFLKFGM; 505,HPDKFLKFGMTPS; 512,FGMTPSKGVLFYG; 486,GGLEDVKRELQEL; 386,ILQIHTKNMKLAD; 524,GPPGCGKTLLAKA; 529,GKTLLAKAIANEC; 336,TLMDGLKQRAHVI; 288,SESNLRKAFEEAE; 148,AYRPIRKGDIFLV; 615,DGMSTKKNVFIIG; 211,DIGGCRKQLAQIK; 217,KQLAQIKEMVELP; 668,RKSPVAKDVDLEF; 754,SDNDIRKYEMFAQ; 651,IPLPDEKSRVAIL; 109,QPCPDVKYGKRIH; 584,ELDSIAKARGGNI; 658,SRVAILKANLRKS; 614,MDGMSTKKNVFII" 18:22790023; 60:22790023; 45:22790023; 231:22790023; 696:22790023; 295:22790023; 312:22790023; 236:22790023; 251:22790023; 502:22790023; 505:22790023; 512:22790023; 486:22790023; 386:22790023; 524:22790023; 529:22790023; 336:22790023; 288:22790023; 148:22790023; 615:22790023; 211:22790023; 217:22790023; 668:22790023; 754:22790023; 651:22790023; 109:22790023; 584:22790023; 658:22790023; 614:22790023