PTM - CPLM Gene Name UniProt ID Type Position Code Pacsin1; Pacsin Q61644 Ubiquitination 51 K Pacsin1; Pacsin Q61644 Ubiquitination 70 K Pacsin1; Pacsin Q61644 Ubiquitination 99 K Pacsin1; Pacsin Q61644 Ubiquitination 120 K Pacsin1; Pacsin Q61644 Ubiquitination 250 K Pacsin1; Pacsin Q61644 Ubiquitination 319 K Pacsin1; Pacsin Q61644 Ubiquitination 411 K

PTM - dbPAF Gene Name UniProt ID Position Type Species Pacsin1; Pacsin Q61644 181 T Mus musculus(Mouse) Pacsin1; Pacsin Q61644 2 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 259 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 260 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 329 T Mus musculus(Mouse) Pacsin1; Pacsin Q61644 334 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 336 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 343 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 345 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 346 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 347 Y Mus musculus(Mouse) Pacsin1; Pacsin Q61644 358 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 362 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 391 Y Mus musculus(Mouse) Pacsin1; Pacsin Q61644 402 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 427 S Mus musculus(Mouse) Pacsin1; Pacsin Q61644 76 S Mus musculus(Mouse)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q61644 K62-ac Acetylation QQLTDWAkRWRQLIE Q61644 S76-p Phosphorylation EkGPQYGsLERAWGA Q61644 T181-p Phosphorylation SKTEQSVtPEQQKKL Q61644 S259-p Phosphorylation HLNLAENssYMHVYR Q61644 S260-p Phosphorylation LNLAENssYMHVYRE Q61644 S343-p Phosphorylation SQAGDRGsVSsyDRG Q61644 S346-p Phosphorylation GDRGsVSsyDRGQTY Q61644 Y347-p Phosphorylation DRGsVSsyDRGQTYA Q61644 S358-p Phosphorylation QTYATEWsDDEsGNP Q61644 S362-p Phosphorylation TEWsDDEsGNPFGGN Q61644 Y391-p Phosphorylation GVRVRALyDYDGQEQ Q61644 S402-p Phosphorylation GQEQDELsFKAGDEL Q61644 S427-p Phosphorylation WCRGRLDsGQLGLYP Q61644 K51-ub Ubiquitination QERAKIEkAYAQQLT Q61644 K70-ub Ubiquitination RWRQLIEkGPQYGsL Q61644 K99-ub Ubiquitination SELHQEVkNSLLNED Q61644 K120-ub Ubiquitination WQKDAYHkQIMGGFK Q61644 K250-ub Ubiquitination KEVLLDIkRHLNLAE Q61644 K319-ub Ubiquitination KKEKQPKkAEGATLS Q61644 K411-ub Ubiquitination KAGDELTkLGEEDEQ

PTM - dbPTM UniProt ID Position Code Type Resources References Q61644 391 Y Phosphorylation Swiss-Prot 1010711 18034455 Q61644 259 S Phosphorylation Phosphositeplus.1010730 20415495 Q61644 260 S Phosphorylation Phosphositeplus.1010730 20415495 Q61644 343 S Phosphorylation Phosphositeplus.1010730 21183079 Q61644 345 S Phosphorylation Phosphositeplus.1010730 21183079 Q61644 346 S Phosphorylation Phosphositeplus.1010730 19737024;20415495;21183079 Q61644 358 S Phosphorylation Phosphositeplus.1010730 20415495;21183079 Q61644 362 S Phosphorylation Phosphositeplus.1010730 20415495 Q61644 402 S Phosphorylation Phosphositeplus.1010730 21183079 Q61644 427 S Phosphorylation Phosphositeplus.1010730 20415495;21183079 Q61644 76 S Phosphorylation Phosphositeplus.1010730 22355135 Q61644 181 T Phosphorylation Phosphositeplus.1010730 19737024;20415495;22355135 Q61644 347 Y Phosphorylation Phosphositeplus.1010730 21183079 Q61644 391 Y Phosphorylation Phosphositeplus.1010730 18034455 Q61644 391 Y Phosphorylation SysPTM 1.1 18034455

PTM - UniProt UniProt ID Position Type PMIDs Q61644 343 Phosphoserine 21183079 Q61644 345 Phosphoserine 21183079 Q61644 358 Phosphoserine 21183079 Q61644 391 Phosphotyrosine 18034455 Q61644 402 Phosphoserine 21183079 Q61644 427 Phosphoserine 21183079

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type Pacsin1 IPI00123613 S 2 _____MpSGSYDEA phosphorylation Pacsin1 IPI00123613 S 259 LNLAENpSSYMHVY phosphorylation Pacsin1 IPI00123613 S 260 NLAENSpSYMHVYR phosphorylation Pacsin1 IPI00123613 S 336 GAVESTpSQAGDRG phosphorylation Pacsin1 IPI00123613 S 345 GDRGSVpSSYDRGQ phosphorylation Pacsin1 IPI00123613 S 346 DRGSVSpSYDRGQT phosphorylation Pacsin1 IPI00123613 S 358 TYATEWpSDDESGN phosphorylation Pacsin1 IPI00123613 S 362 EWSDDEpSGNPFGG phosphorylation Pacsin1 IPI00123613 S 427 CRGRLDpSGQLGLY phosphorylation Pacsin1 IPI00123613 T 181 KTEQSVpTPEQQKK phosphorylation

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID Pacsin1 Mus musculus (Mouse) 411; 120; 250; 51; 319; 70; 99 "411,AGDELTKLGEEDE; 120,QKDAYHKQIMGGF; 250,EVLLDIKRHLNLA; 51,ERAKIEKAYAQQL; 319,KEKQPKKAEGATL; 70,WRQLIEKGPQYGS; 99,ELHQEVKNSLLNE" 411:22790023; 120:22790023; 250:22790023; 51:22790023; 319:22790023; 70:22790023; 99:22790023