PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus Nck2 Bcar1 20637038 mint,intact exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Dok2 20697350,10428862 biogrid,mint,intact exp G G G G G G G G G G G G Nck2 Abi1 11557983 biogrid exp, ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Sorbs1 11557983 biogrid exp, ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Efnb2 15096211 mint,intact exp, ortho G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Dok1 20697350 biogrid,intact exp, ortho G G G G G G G G G G G G G G G G G G G G Nck2 Hnrnpk 11557983 biogrid exp, ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Axin1 Nck2 11557983 biogrid exp, ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Dnm1 11557983 biogrid exp, ortho G G G G G G G G G G G G G G G G G G G G G G Nck2 Efnb1 16511561,11557983 biogrid,mint,intact exp, ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Dvl2 ortho G G G G G G G G G G G G Nck2 Rtn4 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Fam83h ortho G G G G G G G G G G G G G G Nck2 Cd8a ortho G G G G G G Nck2 Erbb3 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Peak1 ortho G G G G Skt Nck2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cd80 ortho G G G G G G Nck2 Pik3ap1 ortho G G G Nck2 Wasf2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Srpk1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Nkd1 ortho G G G G G G Nck2 Meox2 ortho G G G G G G G G G G Nck2 Gab1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Arap1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Trip6 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G 3222402P14Rik Nck2 ortho Nck2 Cd7 ortho G G G G G G Srpk2 Nck2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Rassf7 ortho G G G G G G G G G G G G G G G G G G G G G G G Nck2 Axin2 ortho G G G G G G G G G G G G G G G G G G G G G G G Nck2 Mtnd4 ortho Nck2 Slc25a5 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Abl2 ortho G G G G G G G G G G G G Nck2 Khdrbs1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Tcf4 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Erbb2 ortho G G G G G G G G G G G G Nck2 Git2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cep19 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cbl ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Abi3 ortho Nck2 Cd2 ortho G G G G G G Nck2 Rbm26 ortho Nck2 Sos1 ortho G G G G G G G G G Nck2 Faslg ortho G Nck2 Hist1h1d ortho Nck2 Nck2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cd86 ortho G G G G G G Nck2 Nckipsd ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Drap1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cpsf7 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Wbp11 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Unc45a ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Dyx1c1 ortho G Nck2 Sf3b4 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Caskin2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Tuba1b ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Was ortho G G G G G G G G G Nck2 Lzts2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Chn1 ortho G G G G G G G G G G G G G G Nck2 Brk1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Scrib ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G 9030418K01Rik Nck2 ortho Nck2 Irs1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Ntrk2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Met ortho G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cd8b ortho G G G G G Nck2 Eif3h ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Mink1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Efhc2 ortho Nck2 Csk ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Sema6a Nck2 ortho G G G G G G G G Nck2 Dvl1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Nck1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Ptk2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Tnk2 Nck2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Protein Fat1 Nck2 ortho Nck2 Psmd10 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Atn1 Nck2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Rhou ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cep170 ortho Nck2 Kit ortho G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cytoplasmic FMR1-interacting protein 1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Nhsl1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cblb ortho Nck2 Bcl11a ortho G G G G G G G G G G G G G G G G G G G G Nck2 Cyfip2 ortho G G G G G G G G G G G G G G G G G G G G Nck2 Eef1g ortho Nck2 Fam53c ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Pxn ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Wasf3 ortho G G G G G Nck2 Shb ortho Nck2 Vars2 ortho Nck2 Egfr ortho G G G G G G G G G G G G G G G G G G Nck2 Arhgap32 ortho Nck2 Tnik ortho Nck2 Ugdh ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Top3b ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Dvl3 ortho Nck2 Trim27 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Arhgef7 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Nr5a1 ortho G G G G G G Nck2 Wipf3 ortho Nck2 Hoxc4 ortho G G G G G G G Nck2 Rhov ortho G G G G G Nck2 Baiap2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Nkd2 ortho G G Nck2 Blnk ortho G G G G G G G G G G G G G G G G G G G G G G G Nck2 Axl ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Ptk2b ortho G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Map4k4 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Protein Raph1 Nck2 ortho Nck2 Set ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Spry2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Git1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Pkn2 ortho Nck2 Mapk8ip2 ortho G G G G G G G G G G Nck2 Abi2 ortho Nck2 Homez ortho Nck2 Nphs1 ortho G G G G G Nck2 Mllt4 ortho Nck2 Synpo ortho G G G G G G G G G G G G G G G G G G G G G Nck2 Wasf1 ortho G G G G G G G G G G G G G G G G G Kiaa1522 Nck2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Vps37b ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Trim41 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Lnx1 ortho G G G G G G G G G G G G G G G G G G G G Nck2 Csnk1e ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Rps24 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cdc42bpa ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Lztr1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Fgfr1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Pak2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Fyb Nck2 ortho G G G G G G G G G G G G G G G G G G Nck2 Ilk ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Ddr1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Dock1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Erbb4 ortho Nck2 Lrfn4 ortho Nck2 Wipf2 ortho Nck2 Asap1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Asap2 ortho Nck2 Fxr2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Lcp2 ortho G G G G G G G G G G G G G G Nck2 Flna ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Shc1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Ccdc33 ortho Nck2 Pdcd6ip ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Arf6 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Ubc ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cpsf6 ortho Nck2 Tnpo1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Zbtb7b ortho G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Sorbs2 ortho Nck2 Cd3d ortho G G G G G G G G G Nck2 Cd3e ortho G G G G G Nck2 Rel ortho G G G G Nck2 Xrcc6 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Tnr ortho Nck2 Abl1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Prrc2a ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Cops5 Nck2 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Pdgfrb ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Cep44 ortho Nck2 Lrriq3 ortho Nck2 Arhgef6 ortho Nck2 Lims1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Ttc28 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G Nck2 Wipf1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G Sh3pxd2b Nck2 ortho Nck2 Nckap1 ortho G G G G G G G G G G G G G G G G G G G G G G G G G G G G G G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants P40124 O55033 two hybrid 11557983 direct interaction X 2 O55033 Q8CBW3-5 two hybrid 11557983 direct interaction X 2 O70469 O55033 affinity chromatography technology 20697350 physical association X 2 E9QMJ8 O55033 two hybrid 11557983 direct interaction X 2 P52795 O55033 affinity chromatography technology 11557983 physical association X 2 P52795 O55033 two hybrid 11557983 direct interaction X 2 P97465 O55033 affinity chromatography technology 20697350 physical association X 2 P61979 O55033 two hybrid 11557983 direct interaction X 2 O55033 P39053 two hybrid 11557983 direct interaction X 2 O70469 O55033 anti tag coimmunoprecipitation 10428862 physical association X 2 O55033 pull down 20697350 association C 7 O55033 pull down 20697350 association C 11 P52795 O55033 anti bait coimmunoprecipitation 16511561 physical association X 2 P52800 O55033 pull down 15096211 association X 2 P52800 O55033 peptide array 15096211 association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B Nck2 Hnrnpk MI:0096(pull down) 11557983 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction) (biogrid) BIOGRID_323166 bait prey Nck2 Dnm1 MI:0096(pull down) 11557983 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction) (biogrid) BIOGRID_323165 bait prey Axin1 Nck2 MI:0096(pull down) 11557983 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction) (biogrid) BIOGRID_323163 prey bait Nck2 Efnb1 MI:0007(anti tag coimmunoprecipitation)|MI:0096(pull down)|MI:0007(anti tag coimmunoprecipitation)|MI:0096(pull down) 11557983,16511561 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction) (intact)|(biogrid)|MI:0471(mint)|(biogrid) UniProt_167531|BIOGRID_323160|MINT_80998|BIOGRID_323161 prey|prey|prey|prey bait|bait|bait|bait Nck2 Abi1 MI:0096(pull down) 11557983 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction) (biogrid) BIOGRID_323162 bait prey Nck2 Efnb2 MI:0096(pull down)|MI:0096(pull down)|MI:0096(pull down)|MI:0096(pull down) 15096211 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0914(association)|MI:0914(association)|MI:0914(association)|MI:0914(association) (intact)|(intact)|MI:0471(mint)|MI:0471(mint) UniProt_163805|UniProt_163806|MINT_80269|MINT_80270 unspecified role|unspecified role|unspecified role|unspecified role unspecified role|unspecified role|unspecified role|unspecified role Nck2 Cap1 MI:0096(pull down) 11557983 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0407(direct interaction) (biogrid) BIOGRID_323164 bait prey Nck2 Dok2 MI:0096(pull down)|MI:0096(pull down)|MI:0004(affinity chromatography technology)|MI:0096(pull down)|MI:0096(pull down)|MI:0096(pull down) 10428862,20697350 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0914(association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0914(association)|MI:0914(association) (intact)|(intact)|(biogrid)|MI:0471(mint)|(intact)|(intact) UniProt_108793|UniProt_161693|BIOGRID_322850|MINT_59920|UniProt_185043|UniProt_172902 prey|prey|prey|prey|prey|prey bait|bait|bait|bait|bait|bait Nck2 Dok1 MI:0096(pull down)|MI:0004(affinity chromatography technology)|MI:0096(pull down)|MI:0096(pull down) 20697350 taxid:10090(Mus musculus) taxid:10090(Mus musculus) MI:0914(association)|MI:0915(physical association)|MI:0914(association)|MI:0914(association) (intact)|(biogrid)|(intact)|(intact) UniProt_108792|BIOGRID_322843|UniProt_172901|UniProt_185042 prey|prey|prey|prey bait|bait|bait|bait

PPI - HINT Gene A Gene B PMID Method Quality AXIN1 NCK2 11557983 0018 LC NCK2 DNM1 11557983 0018 LC NCK2 EFNB1 11557983,11557983 0018,0004 LC NCK2 EFNB2 15096211,15096211 0096,0081 LC NCK2 HNRNPK 11557983 0018 LC NCK2 BCAR1 20637038 0416 LC NCK2 SORBS1 11557983 0018 LC NCK2 ABI1 11557983 0018 LC NCK2 DOK2 10428862,20697350,20697350 0007,0004,0096 LC NCK2 EFNB1 11557983,11557983,16511561 0018,0004,0006 LC NCK2 EFNB2 15096211,15096211 0096,0081 LC NCK2 DOK1 20697350,20697350 0004,0096 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID O55033 O35625 NCK2 AXIN1 0.309 11557983 P39053 O55033 DNM1 NCK2 0.309 11557983 P61979 O55033 HNRNPK NCK2 0.309 11557983 Q62417 O55033 SORBS1 NCK2 0.309 11557983 Q8CBW3 O55033 ABI1 NCK2 0.309 11557983 O70469 O55033 DOK2 NCK2 0.472 10428862,20697350 P97465 O55033 DOK1 NCK2 0.332 20697350 P52795 O55033 EFNB1 NCK2 0.569 11557983,16511561 P52800 O55033 EFNB2 NCK2 0.376 15096211 Q3TTA7 O55033 CBLB NCK2 0.126 27474268 Q3U1F9 O55033 PAG1 NCK2 0.126 26512138