PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus Dlg4 Sharpin 11178875 intact exp G G G G G G Grip1 Sharpin 11178875 intact exp Sharpin Shank3 23897824 biogrid exp G G G G G G Sharpin Shank1 11178875 i2d,intact exp, ortho G G G Sharpin Sharpin 11178875 i2d,intact exp, ortho G G G G G G G G Tekt4 Sharpin ortho Surf6_predicted Sharpin ortho G G G G G G G Psmd1 Sharpin ortho G G G G G G G G Card15_predicted Sharpin ortho Tnfrsf1a Sharpin ortho G G G G G G G G Eya2 Sharpin ortho Sharpin Itgad ortho G Adcyap1 Sharpin ortho G G G Stk24 Sharpin ortho G G G G G G G G Protein Rnf20 Sharpin ortho Sharpin Shank2 ortho G G G G G G G Itga1 Sharpin ortho G G G G G Itgal Sharpin ortho Lipf Sharpin ortho Rbck1 Sharpin ortho G G G G G G G PTEN/MMAC1 Sharpin ortho G G G G G Itgb1 Sharpin ortho G G G G G G G G RGD1563421_predicted Sharpin ortho Rnf31_predicted Sharpin ortho Chuk_predicted Sharpin ortho G G G G G G G G Cebpa Sharpin ortho Aurkb Sharpin ortho G G G G Sharpin Aifm1 ortho G G G G G G G G Uchl1 Sharpin ortho G G G G G Tnf Sharpin ortho Il1a Sharpin ortho G LOC64038 protein, Protein Otulin Sharpin ortho LOC683206 Sharpin ortho Txn2 Sharpin ortho G G G G G G G G Ikbkg Sharpin ortho Traf2_predicted Sharpin ortho Protein Tfap2c, Transcription factor AP-2, gamma Sharpin ortho Lymphotoxin B receptor, Lymphotoxin beta receptor (TNFR superfamily, member 3), Protein Ltbr Sharpin ortho G G G G G G G G Scn11a Sharpin ortho Bdkrb2 Sharpin ortho G G Sharpin Ikbkb ortho G G G G G G G G Capn13 Sharpin ortho G Sharpin Itgam ortho G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q9EQL9 - 11178875 - X 2 Q9EQL9 Q9EQL9 - 11178875 - X 2 Q9EQL9 - 11178875 - X 2 Q9EQL9 Q9EQL9 - 11178875 - X 2 P0CG48 Q9EQL9 pull down 22253853 direct interaction X 2 Q9EQL9 affinity chromatography technology 23897824 physical association X 2 Q96EP0 Q9EQL9 affinity chromatography technology 22253853 physical association X 2 Q96EP0 Q9EQL9 affinity chromatography technology 22253853 physical association X 2 Q9EQL9 Q9WV48 coimmunoprecipitation 11178875 association X 2 Q9EQL9 Q9EQL9 coimmunoprecipitation 11178875 association Y 1 P17301 Q9EQL9 pull down 21947080 physical association X 2 Q9EQL9 Q9WV48 anti bait coimmunoprecipitation 11178875 physical association X 2 Q9EQL9 Q9WV48 anti bait coimmunoprecipitation 11178875 physical association X 2 Q9EQL9 Q9WV48 confocal microscopy 11178875 colocalization X 2 Q9EQL9 Q9EQL9 anti tag coimmunoprecipitation 11178875 physical association X 2 Q9EQL9 Q9EQL9 anti tag coimmunoprecipitation 11178875 physical association X 2 Q9EQL9 Q9WV48 two hybrid 11178875 physical association X 2 Q9EQL9 Q9WV48 two hybrid 11178875 physical association X 2 Q9EQL9 Q9WV48 confocal microscopy 11178875 colocalization X 2 Q9EQL9 Q9WV48 anti bait coimmunoprecipitation 11178875 physical association X 2 P31016 Q9EQL9 confocal microscopy 11178875 colocalization X 2 P31016 Q9EQL9 anti bait coimmunoprecipitation 11178875 physical association X 2 P97879 Q9EQL9 anti bait coimmunoprecipitation 11178875 physical association X 2 Q9EQL9 P56199 pull down 21947080 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B Dlg4 Sharpin MI:0018(two hybrid)|MI:0018(two hybrid) 11178875 taxid:10116(Rattus norvegicus) taxid:10116(Rattus norvegicus) MI:0403(colocalization)|MI:0915(physical association) (intact)|(intact) UniProt_180513|UniProt_180503 neutral component|prey neutral component|bait Sharpin Shank1 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 11178875 taxid:10116(Rattus norvegicus) taxid:10116(Rattus norvegicus) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0403(colocalization)|MI:0915(physical association)|MI:0403(colocalization) (intact)|(intact)|(intact)|(intact)|(intact)|(intact)|(intact) UniProt_180504|UniProt_180497|UniProt_180500|UniProt_180498|UniProt_180512|UniProt_180499|UniProt_180511 bait|prey|bait|prey|neutral component|prey|neutral component prey|bait|prey|bait|neutral component|bait|neutral component Sharpin Shank3 MI:0686(unspecified method) 23897824 taxid:10116(Rattus norvegicus) taxid:10116(Rattus norvegicus) MI:0915(physical association) (biogrid) BIOGRID_337637 bait prey Grip1 Sharpin MI:0018(two hybrid) 11178875 taxid:10116(Rattus norvegicus) taxid:10116(Rattus norvegicus) MI:0915(physical association) (intact) UniProt_180505 prey bait

PPI - HINT Gene A Gene B PMID Method Quality SHARPIN SHARPIN 11178875,11178875 0019,0007 LC SHARPIN SHANK1 11178875,11178875,11178875,11178875 0019,0018,0663,0006 LC DLG4 SHARPIN 11178875,11178875 0663,0006 LC GRIP1 SHARPIN 11178875 0006 LC SHARPIN SHARPIN 11178875,11178875 0019,0007 LC SHARPIN SHANK3 23897824 0004 LC SHARPIN SHANK1 11178875,11178875,11178875,11178875 0019,0018,0663,0006 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q9EQL9 Q96EP0 SHARPIN RNF31 0.376 22253853 Q9EQL9 P0CG48 SHARPIN UBC 0.309 22253853 Q9EQL9 P17301 SHARPIN ITGA2 0.21 21947080 Q9EQL9 P56199 SHARPIN ITGA1 0.21 21947080 Q9JLU4 Q9EQL9 SHANK3 SHARPIN 0.21 23897824