PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus CG5281-RA Nedd4 25294943 intact exp G G CG8180, isoform A, CG8180, isoform C, LD38554p Nedd4 22036573 biogrid exp G G G G G Ino80 Nedd4 25294943 intact exp G G G G G G G G comm Nedd4 17074801,unassigned4,16531238 biogrid,intact,dip exp G G G G G G G G Nedd4 Usp10 25294943 intact exp G G G G G G G G G Kuzbanian, isoform A, Kuzbanian, isoform B Nedd4 25294943 intact exp G G G G G G G Ten-m Nedd4 25294943 intact exp G G G G G G G Formin-like protein CG32138 Nedd4 22036573 biogrid exp G G G G G G dally Nedd4 25294943 intact exp G G G G G G G G Nedd4 Su(dx) 22036573 biogrid exp G G G G G G G G G Amp Nedd4 15710747 biogrid,intact exp G G G G G G G G G sty Nedd4 22036573 biogrid exp G G G G G G G G G N Nedd4 i2d exp, ortho G G G G ptc Nedd4 15710747 biogrid,i2d,intact exp, ortho EG:100G10.3 Nedd4 ortho G G G G G G G G G flr Nedd4 ortho G G G G G G G G G Probable tRNA (guanine(26)-N(2))-dimethyltransferase Nedd4 ortho G G G G G G G G G AMPdeam Nedd4 ortho G G G G G G G G G eff Nedd4 ortho G G G G G G G G G CG6182 Nedd4 ortho G G G G G G G G G Nedd4 LD16419p, RhoGDI, isoform A, RhoGDI, isoform B ortho G G G G G G G G G Nacalpha Nedd4 ortho G G G G G G G G G Hyperkinetic, isoform F Nedd4 ortho G G G emb Nedd4 ortho G G G G G G G G G LD29171p, Sec71 ortholog Nedd4 ortho G G G G G G G G G CG1275, isoform A, CG1275-PA, isoform A, LD36721p Nedd4 ortho G G G G G G G G G RE34924p, Rpb5, isoform A, Rpb5, isoform B Nedd4 ortho G G G G G G G G G Updo Nedd4 ortho G G G G G G G G G porin Nedd4 ortho G G G G G G G G G Medea Nedd4 ortho G G G G G G G G G RpII140 Nedd4 ortho G G G G G G G G G AT16033p, CG3473, isoform A, CG3473, isoform B Nedd4 ortho Elp2 Nedd4 ortho G G G G G G G G CG7339, isoform A, CG7339, isoform B, GM16372p Nedd4 ortho G G isopeptidase-T-3 Nedd4 ortho G G G G G G G G CG6796, RE15573p Nedd4 ortho G G G G Adk2 Nedd4 ortho G G G G G G G G G mRpL19 Nedd4 ortho G G G G G G G G G PyK Nedd4 ortho G G G G G G G G G beta'Cop Nedd4 ortho G G G G G G G G G CG5196-RA Nedd4 ortho G G G G G G G G CG5919 protein, CG5919, isoform A, CG5919, isoform B, CG5919-PA, GM08271p Nedd4 ortho G G G G G G G G G asf1 Nedd4 ortho G G G Nedd4 Folylpolyglutamate synthase ortho G G G MED28 Nedd4 ortho G G G G G G G CG5823, isoform A, CG5823, isoform B, HL05730p, RE16955p Nedd4 ortho G G G G G G G G G 14-3-3epsilon Nedd4 ortho G G G G G G G G G Nedd8 Nedd4 ortho G G G G G G G G G CG11089-RA Nedd4 ortho Pgi Nedd4 ortho G G G G G G G G G Prosalpha7 Nedd4 ortho G G G G G G G G G CG8549, isoform B, CG8549-PA Nedd4 ortho G G G G G G G G G rad23 Nedd4 ortho G G G G G G G G G Nedd4 Uncharacterized protein CG1785 ortho G G G G G G Cdk5 Nedd4 ortho G G G G G G G G G Chc Nedd4 ortho G G G G G G G G G Mtap Nedd4 ortho G G G G G G G G G CG14669, isoform A, CG14669, isoform B Nedd4 ortho Nervana 3, isoform E Nedd4 ortho G G G G G G G Nedd4 G-beta-5 ortho G G G G G NHP2 Nedd4 ortho G G G G G G G G G Trip1 Nedd4 ortho G G G G G G G G G betaCop Nedd4 ortho G G G G G G G G G Pglym87 Nedd4 ortho CG6994 Nedd4 ortho G G G G G G G G G Nedd4 CG2972, isoform B, CG2972-PA, LD17927p ortho G G G G G G G Nedd4 CG32672-PA, LD05816p ortho G G G G G G G G G Desaturase 2, Fatty acid desaturase 2, IP02593p Nedd4 ortho CG10652-PA, isoform A, RE25263p, Ribosomal protein L30, isoform B, Ribosomal protein L30, isoform E Nedd4 ortho G G G G G G G G G CG12024, isoform A, GH08896p Nedd4 ortho G G G G G G G G G Jetlag, isoform A Nedd4 ortho G G exd Nedd4 ortho G G G G G G G G G Nurf-38 Nedd4 ortho G G G G G G G G G CG4573 Nedd4 ortho G Nedd4 Keren ortho G G G G G G G G G Nedd4 Translation machinery-associated protein 16 homolog ortho G G G G G G G G G Hrs Nedd4 ortho G G G G G G G G G Spt5 Nedd4 ortho G G G G G G G G G phl Nedd4 ortho G G G G G G G G G Nedd4 AdSS ortho G G G G G G G G G pod1-RC Nedd4 ortho G G G G G G G G G prp8 Nedd4 ortho G G G G G G G G G Act5C Nedd4 ortho G G G G G G G G G Klp68D Nedd4 ortho G G G G G G G G G Leukotriene A(4) hydrolase, LTA-4 hydrolase Nedd4 ortho G G G G G G G G G Rpn11 Nedd4 ortho G G G G G G G G G Ubi-p63E Nedd4 ortho G G G G G G G G G Nedd4 Ubc7 ortho G G G G G G G G G gny Nedd4 ortho Nedd4 LD25378p, Minidiscs, Minidiscs, isoform A, Minidiscs, isoform C, Minidiscs, isoform D ortho G G G G G G G G Tor Nedd4 ortho G G G G G G G G G Vha55 Nedd4 ortho G G G G G G G G G His3, His3:CG31613, His3:CG33803, His3:CG33806, His3:CG33809, His3:CG33812, His3:CG33815, His3:CG33818, His3:CG33821, His3:CG33824, His3:CG33827, His3:CG33830, His3:CG33833, His3:CG33836, His3:CG33839, His3:CG33842, His3:CG33845, His3:CG33848, His3:CG33851, His3:CG33854, His3:CG33857, His3:CG33860, His3:CG33863, His3:CG33866 Nedd4 ortho 14-3-3zeta Nedd4 ortho G G G G G G G para Nedd4 ortho G G ial Nedd4 ortho G MAPk-Ak2 Nedd4 ortho G G G G G G G G G Hsc70-3 Nedd4 ortho G G G G G G G G G RpII215 Nedd4 ortho G G G G G G G G G ico Nedd4 ortho G G G G G G G G G Rpb10 Nedd4 ortho G G G G G G G G G Nedd4 Coatomer subunit alpha ortho G G G G G G G G G gammaCop Nedd4 ortho G G G G G G G G G Nedd4 Nedd4 ortho G G G G G G G G G me31B Nedd4 ortho G G G G G G G G G Npc2a Nedd4 ortho G G G G G G G G G UbcE2H Nedd4 ortho G G G G G G G G G Glutamate oxaloacetate transaminase 1, isoform B Nedd4 ortho G G G G G G G G G cdc2c Nedd4 ortho G G G IP02321p, IP02323p, Rpb7 Nedd4 ortho G G G G G G G G G RpS27A Nedd4 ortho G G G G G G G G G Pka-C3 Nedd4 ortho G G G G G G G Roc2-RA Nedd4 ortho G G G G G G G G G Acetyltransferase component of pyruvate dehydrogenase complex Nedd4 ortho G G G G G G G G G Nedd4 Mfe2 ortho G G G G G G G G G Sec23 Nedd4 ortho G G G G G G G G G Pka-C1 Nedd4 ortho G G G G G G G G G CG2720-PA, LD03220p Nedd4 ortho G G G G G G G G G Rpn10 Nedd4 ortho G G G G G G G G G Mvl Nedd4 ortho G G G G G G G G G UbcD4 Nedd4 ortho G G G G G G G G G Arp2 Nedd4 ortho G G G G G G G G G CG32850-PA, RE41137p Nedd4 ortho G G G G G G G G Mob3 Nedd4 ortho G G G G G G G G G AMP-activated protein kinase alpha subunit, isoform A, AMP-activated protein kinase alpha subunit, isoform B, AMP-activated protein kinase alpha subunit, isoform C, EG:132E8.2 protein, FI03728p, SNF1A/AMP-activated protein kinase Nedd4 ortho G G G G G G G G G Pdi Nedd4 ortho G G G G G G G G G Aats-ala Nedd4 ortho G G G G G G G G G RPB3 Nedd4 ortho G G G G G G G G G Ubc12 Nedd4 ortho G G G G G G G G G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants Q9VVI3 M9NE67 - 15620649 - X 2 Q9VVI3 Q7KLE5 - 15710747 - X 2 Q9VVI3 Q24139 fluorescence technology 16531238 direct interaction X 2 Q9VVI3 Q24139 nuclear magnetic resonance 16531238 direct interaction X 2 Q9VVI3 Q24139 fluorescence technology 16531238 direct interaction X 2 Q9VVI3 anti tag coimmunoprecipitation 25294943 association C 47 Q9VVI3 Q8IGE8 two hybrid fragment pooling approach 15710747 physical association X 2 Q9VVI3 Q24139 experimental interaction detection - physical association X 2 Q9VVI3 Q7KLE5 two hybrid fragment pooling approach 15710747 physical association X 2

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q9VVI3 O44783 NEDD4 STY 0.126 22036573 Q9Y0H4 Q9VVI3 SU(DX) NEDD4 0.126 22036573 Q9VVI3 O61307 NEDD4 TEN-M 0.236 25294943 Q9VVI3 Q9VDY1 NEDD4 INO80 0.126 25294943 Q9VVI3 Q9VGN4 NEDD4 SALS 0.126 25294943 Q9VVI3 Q9VJW9 NEDD4 Q9VJW9 0.126 25294943 Q9VVI3 Q9VUC6 NEDD4 Q9VUC6 0.126 22036573 Q9VVI3 Q7KLE5 NEDD4 AMPH 0.126 15710747 Q9VVI3 Q8IGE8 NEDD4 PTC 0.126 15710747 Q9W0L7 Q9VVI3 CG13903 NEDD4 0.126 25294943 Q9VVI3 P18502 NEDD4 PTC 0.183 15710747 Q9VVI3 Q24139 NEDD4 COMM 0.671 unassigned4,17074801,16531238 Q9VVI3 Q24114 NEDD4 DALLY 0.126 25294943