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Basic Information Integrated Annotations

Tag Content
iUUCD ID IUUC-Sce-106355
UUCD1 version UUC-SaC-00261
Ensembl Protein ID YPR066W
UniProt Accession Q99344; D6W470; E9P8X1; UBA3_YEAST
Genbank Protein ID CAA76517.1; CAA94974.1; CAA89183.1; AAT92800.1; DAA11486.1
Protein Name NEDD8-activating enzyme E1 catalytic subunit; RUB1-activating enzyme E1; Ubiquitin-activating enzyme E1 3; Ubiquitin-like protein-activating enzyme
Genbank Nucleotide ID Y16891; Z71255; Z49219; AY692781; BK006949
Gene Name UBA3; YPR066W; YP9499.21
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
YPR066W YPR066W YPR066W
Annotation
mRNA Expression
GEOFFGED
Protein-protein Interaction
IIDiRefIndexHINTMentha
Post-translational Modifications (PTMs)
BioGRID
Protein Expression/Proteomics
GPMDB
Status Reviewed
Details
Family Domain References (PMIDs)
E1 E1_ThiF 9545234; 10993680
Classification
Family E-value Score Start End
E1 8.80e-81 270.6 3 184
Active Site
Position(s) Description Evidence
168 Glycyl thioester intermediate {ECO:0000255|PROSITE-ProRule:PRU10132}
Domain Profile

   E1

   S: 19   akvllvGagglGcEllKnLvlaGv.gsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnveaheesiteleeeivFfkkfdvVvlaldn 119
    +k+l++GagglGcE+lKnL+++ + +++++vD dt+e++nlnrqFLf+++d+gk+KA+vaa+ ++ ++p+ +v+ah +++t+l + F+k+f++++++ld
   Q: 3 CKILVLGAGGLGCEILKNLTMLSFvKQVHIVDIDTIELTNLNRQFLFCDKDIGKPKAQVAAQYVNTRFPQLEVVAHVQDLTTLPPS--FYKDFQFIISGLDA 102
    89*********************99*************************************************************..************** PP
   S: 120 rearrkvnrlclna........rvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlketpnaaehtiewa 190
    +e rr +n+ ++++ ++p+i++gt+Gl+G+v++ii+g+t+c++cs +++q+t+P+ct++++p+++eh++e++
   Q: 103 IEPRRFINETLVKLtlesnyeiCIPFIDGGTEGLKGHVKTIIPGITACWECSIdtlPSQQDTVPMCTIANNPRCIEHVVEYV 184
    *****************************************************9***************************9 PP
   

Organism Saccharomyces cerevisiae
Functional Description
(View)

Functional Description



     Catalytic subunit of the dimeric UBA3-ULA1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBC12.
Catalytic subunit of the dimeric UBA3-ULA1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBC12.
Protein Sequence
(Fasta)
MDCKILVLGA GGLGCEILKN LTMLSFVKQV HIVDIDTIEL TNLNRQFLFC DKDIGKPKAQ 60
VAAQYVNTRF PQLEVVAHVQ DLTTLPPSFY KDFQFIISGL DAIEPRRFIN ETLVKLTLES 120
It may take some time, please wait.

Protein Fasta Sequence



>IUUC-Sce-106355|E1,E1_ThiF|Saccharomyces cerevisiae
Please wait for a moment...
Nucleotide Sequence
(Fasta)
ATGGACTGCA AGATATTGGT GTTAGGCGCA GGTGGTCTAG GATGCGAAAT CCTGAAGAAT 60
TTGACAATGT TAAGCTTTGT TAAACAGGTT CATATTGTAG ATATAGACAC AATCGAGCTA 120
It may take some time, please wait.

Nucleotide Fasta Sequence



>IUUC-Sce-106355|E1,E1_ThiF|Saccharomyces cerevisiae
Please wait for a moment...
Sequence Source Ensembl
Keyword

KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd
IPR030468--Uba3
IPR033127--UBQ-activ_enz_E1_Cys_AS

PROSITE

PS00865--UBIQUITIN_ACTIVAT_2

Pfam

PF00899--ThiF

Gene Ontology

GO:0016881--F:acid-amino acid ligase activity
GO:0005524--F:ATP binding
GO:0019781--F:NEDD8 activating enzyme activity
GO:0045116--P:protein neddylation

KEGG sce:YPR066W
Orthology
iUUCD ID Species Identity E-value Score
IUUC-Ata-001124 Aegilops tauschii 36.53 1.00e-39 155.00
IUUC-Aml-001578 Ailuropoda melanoleuca 42.36 9.00e-71 258.00
IUUC-Atr-002591 Amborella trichopoda 36.24 1.00e-41 162.00
IUUC-Apl-003671 Anas platyrhynchos 43.09 5.00e-72 263.00
IUUC-Aca-004749 Anolis carolinensis 44.16 9.00e-74 268.00
IUUC-Aly-005747 Arabidopsis lyrata 40.07 1.00e-52 198.00
IUUC-Ath-006623 Arabidopsis thaliana 39.74 2.00e-52 197.00
IUUC-Ago-007934 Ashbya gossypii 51.13 2.00e-89 320.00
IUUC-Acl-008047 Aspergillus clavatus 39.60 4.00e-62 229.00
IUUC-Afl-008615 Aspergillus flavus 32.42 2.00e-33 134.00
IUUC-Afu-008996 Aspergillus fumigatus 39.26 3.00e-56 210.00
IUUC-Ani-009281 Aspergillus nidulans 37.07 7.00e-35 139.00
IUUC-Ang-009539 Aspergillus niger 39.93 2.00e-52 197.00
IUUC-Aor-010151 Aspergillus oryzae 37.30 1.00e-50 191.00
IUUC-Ate-010502 Aspergillus terreus 40.53 2.00e-54 204.00
IUUC-Ame-011837 Astyanax mexicanus 43.65 4.00e-65 239.00
IUUC-Bgr-012068 Blumeria graminis 40.00 3.00e-54 203.00
IUUC-Bta-013003 Bos taurus 42.68 1.00e-71 261.00
IUUC-Bci-013983 Botrytis cinerea 40.53 1.00e-57 214.00
IUUC-Bdi-014429 Brachypodium distachyon 35.89 3.00e-41 160.00
IUUC-Bol-015813 Brassica oleracea 36.05 5.00e-43 166.00
IUUC-Bra-018023 Brassica rapa 36.46 2.00e-42 164.00
IUUC-Cel-018684 Caenorhabditis elegans 38.71 3.00e-59 220.00
IUUC-Cja-019200 Callithrix jacchus 43.22 1.00e-70 258.00
IUUC-Cfa-021038 Canis familiaris 42.68 2.00e-71 260.00
IUUC-Cre-022778 Chlamydomonas reinhardtii 38.56 1.00e-61 228.00
IUUC-Csa-024079 Chlorocebus sabaeus 42.68 1.00e-71 261.00
IUUC-Cho-025052 Choloepus hoffmanni 45.65 2.00e-57 214.00
IUUC-Cin-025113 Ciona intestinalis 40.38 6.00e-57 212.00
IUUC-Csv-026231 Ciona savignyi 40.58 6.00e-64 236.00
IUUC-Cgl-026535 Colletotrichum gloeosporioides 41.06 5.00e-59 219.00
IUUC-Cne-026850 Cryptococcus neoformans 42.52 6.00e-63 232.00
IUUC-Cme-027275 Cyanidioschyzon merolae 31.55 8.00e-47 179.00
IUUC-Dre-028751 Danio rerio 42.02 4.00e-63 233.00
IUUC-Dno-029571 Dasypus novemcinctus 42.68 3.00e-71 260.00
IUUC-Dor-030395 Dipodomys ordii 42.36 3.00e-71 260.00
IUUC-Dse-031329 Dothistroma septosporum 36.22 5.00e-52 196.00
IUUC-Dme-031776 Drosophila melanogaster 39.87 4.00e-59 219.00
IUUC-Ete-032894 Echinops telfairi 40.96 2.00e-31 127.00
IUUC-Eca-034389 Equus caballus 42.68 2.00e-71 261.00
IUUC-Eeu-035152 Erinaceus europaeus 39.49 6.00e-60 222.00
IUUC-Fca-036032 Felis catus 42.68 2.00e-71 261.00
IUUC-Fal-037506 Ficedula albicollis 43.31 6.00e-72 262.00
IUUC-Fox-037871 Fusarium oxysporum 38.57 1.00e-48 184.00
IUUC-Fso-038350 Fusarium solani 42.67 3.00e-58 217.00
IUUC-Gmo-039246 Gadus morhua 44.12 2.00e-64 237.00
IUUC-Ggr-039859 Gaeumannomyces graminis 41.28 3.00e-60 223.00
IUUC-Gga-040843 Gallus gallus 42.12 4.00e-67 246.00
IUUC-Gac-042610 Gasterosteus aculeatus 43.04 3.00e-64 237.00
IUUC-Gma-043031 Glycine max 36.59 8.00e-44 169.00
IUUC-Ggo-044788 Gorilla gorilla 42.86 1.00e-70 258.00
IUUC-Hsa-045849 Homo sapiens 42.99 9.00e-72 261.00
IUUC-Hvu-047819 Hordeum vulgare 36.59 3.00e-42 163.00
IUUC-Itr-047863 Ictidomys tridecemlineatus 42.36 2.00e-71 261.00
IUUC-Kpa-049393 Komagataella pastoris 43.71 3.00e-66 243.00
IUUC-Lch-049471 Latimeria chalumnae 43.55 4.00e-65 239.00
IUUC-Lpe-051297 Leersia perrieri 35.89 5.00e-48 183.00
IUUC-Loc-052910 Lepisosteus oculatus 43.69 1.00e-64 238.00
IUUC-Lma-053181 Leptosphaeria maculans 39.02 2.00e-61 227.00
IUUC-Laf-054314 Loxodonta africana 42.01 1.00e-69 255.00
IUUC-Mcc-054870 Macaca mulatta 42.99 9.00e-72 261.00
IUUC-Mor-056919 Magnaporthe oryzae 40.27 4.00e-58 216.00
IUUC-Mpo-057614 Magnaporthe poae 41.61 6.00e-58 216.00
IUUC-Mtr-058110 Medicago truncatula 37.23 1.00e-43 168.00
IUUC-Mla-058949 Melampsora laricipopulina 43.19 1.00e-64 238.00
IUUC-Mga-059455 Meleagris gallopavo 42.77 2.00e-71 261.00
IUUC-Mvi-060270 Microbotryum violaceum 38.74 1.00e-58 218.00
IUUC-Mmr-061155 Microcebus murinus 42.04 5.00e-70 256.00
IUUC-Mdo-062037 Monodelphis domestica 42.68 8.00e-71 258.00
IUUC-Mmu-063670 Mus musculus 42.68 1.00e-71 261.00
IUUC-Mac-064711 Musa acuminata 35.42 1.00e-39 155.00
IUUC-Mpu-066734 Mustela putorius furo 42.41 3.00e-71 259.00
IUUC-Mlu-067068 Myotis lucifugus 42.36 6.00e-71 259.00
IUUC-Nfi-068349 Neosartorya fischeri 39.93 1.00e-57 215.00
IUUC-Ncr-068626 Neurospora crassa 39.40 6.00e-55 206.00
IUUC-Nle-069366 Nomascus leucogenys 42.99 4.00e-71 259.00
IUUC-Ont-072196 Oreochromis niloticus 42.72 3.00e-62 230.00
IUUC-Oan-073511 Ornithorhynchus anatinus 41.94 3.00e-69 253.00
IUUC-Ocu-073773 Oryctolagus cuniculus 42.99 6.00e-72 262.00
IUUC-Oba-075726 Oryza barthii 35.79 2.00e-45 174.00
IUUC-Obr-076286 Oryza brachyantha 35.89 3.00e-48 184.00
IUUC-Ogu-078473 Oryza glumaepatula 36.05 2.00e-45 174.00
IUUC-Oin-079393 Oryza indica 36.24 6.00e-49 186.00
IUUC-Olo-080446 Oryza longistaminata 36.65 1.00e-46 178.00
IUUC-Ome-081737 Oryza meridionalis 36.65 1.00e-46 178.00
IUUC-Oni-082853 Oryza nivara 35.79 2.00e-45 174.00
IUUC-Opu-083590 Oryza punctata 36.24 7.00e-49 186.00
IUUC-Oru-084184 Oryza rufipogon 35.79 2.00e-45 174.00
IUUC-Osa-085402 Oryza sativa 36.24 6.00e-49 186.00
IUUC-Ola-086671 Oryzias latipes 41.83 7.00e-57 212.00
IUUC-Olu-087738 Ostreococcus lucimarinus 35.19 2.00e-44 171.00
IUUC-Oga-088101 Otolemur garnettii 42.99 6.00e-72 262.00
IUUC-Oar-090293 Ovis aries 42.01 2.00e-69 254.00
IUUC-Ptr-090503 Pan troglodytes 42.99 9.00e-72 261.00
IUUC-Pan-092477 Papio anubis 41.40 5.00e-69 253.00
IUUC-Psi-093442 Pelodiscus sinensis 42.68 4.00e-71 259.00
IUUC-Pma-094247 Petromyzon marinus 40.82 2.00e-60 224.00
IUUC-Pno-094780 Phaeosphaeria nodorum 37.92 2.00e-61 227.00
IUUC-Ppa-095338 Physcomitrella patens 39.09 7.00e-62 229.00
IUUC-Pfo-097137 Poecilia formosa 43.69 1.00e-62 231.00
IUUC-Pab-098060 Pongo abelii 43.31 2.00e-64 237.00
IUUC-Pop-099470 Populus trichocarpa 38.28 5.00e-50 189.00
IUUC-Pca-100500 Procavia capensis 37.74 1.00e-46 178.00
IUUC-Ppe-102008 Prunus persica 34.84 9.00e-40 155.00
IUUC-Pva-103095 Pteropus vampyrus 50.00 2.00e-46 177.00
IUUC-Pgr-103666 Puccinia graminis 43.19 5.00e-65 239.00
IUUC-Ptt-103901 Puccinia triticina 43.85 2.00e-66 244.00
IUUC-Pte-104198 Pyrenophora teres 38.94 7.00e-61 225.00
IUUC-Pyt-104792 Pyrenophora triticirepentis 39.00 3.00e-59 220.00
IUUC-Rno-105446 Rattus norvegicus 42.68 1.00e-71 261.00
IUUC-Sha-106664 Sarcophilus harrisii 42.68 1.00e-70 258.00
IUUC-Sja-107982 Schizosaccharomyces japonicus 42.35 2.00e-67 247.00
IUUC-Spo-108345 Schizosaccharomyces pombe 39.56 4.00e-66 243.00
IUUC-Ssl-108540 Sclerotinia sclerotiorum 38.33 7.00e-50 189.00
IUUC-Smo-109133 Selaginella moellendorffii 38.36 5.00e-60 223.00
IUUC-Sit-109724 Setaria italica 36.24 1.00e-41 162.00
IUUC-Sly-110916 Solanum lycopersicum 38.08 2.00e-50 190.00
IUUC-Stu-112236 Solanum tuberosum 38.08 3.00e-50 190.00
IUUC-Sar-113044 Sorex araneus 42.99 1.00e-71 261.00
IUUC-Sbi-114276 Sorghum bicolor 36.24 2.00e-41 161.00
IUUC-Sre-114963 Sporisorium reilianum 41.47 8.00e-61 225.00
IUUC-Ssc-116301 Sus scrofa 42.01 1.00e-69 254.00
IUUC-Tgu-117265 Taeniopygia guttata 43.31 6.00e-72 262.00
IUUC-Tru-117526 Takifugu rubripes 43.63 6.00e-64 236.00
IUUC-Tsy-119483 Tarsius syrichta 42.36 4.00e-71 259.00
IUUC-Tni-120363 Tetraodon nigroviridis 43.45 1.00e-65 241.00
IUUC-Tca-121731 Theobroma cacao 35.89 2.00e-41 161.00
IUUC-Tre-122221 Trichoderma reesei 38.93 8.00e-50 188.00
IUUC-Tvi-122352 Trichoderma virens 41.67 1.00e-58 218.00
IUUC-Tae-123864 Triticum aestivum 36.59 1.00e-42 165.00
IUUC-Tur-126070 Triticum urartu 34.51 2.00e-32 130.00
IUUC-Tme-127199 Tuber melanosporum 41.22 6.00e-58 216.00
IUUC-Tbe-128076 Tupaia belangeri 42.36 4.00e-71 259.00
IUUC-Ttr-128695 Tursiops truncatus 52.25 1.00e-32 132.00
IUUC-Uma-129638 Ustilago maydis 40.80 2.00e-62 231.00
IUUC-Vda-129963 Verticillium dahliae 41.53 6.00e-53 199.00
IUUC-Vpa-130637 Vicugna pacos 36.57 1.00e-53 201.00
IUUC-Vvi-131613 Vitis vinifera 35.54 5.00e-40 156.00
IUUC-Xtr-132924 Xenopus tropicalis 42.68 5.00e-63 233.00
IUUC-Xma-133356 Xiphophorus maculatus 44.01 1.00e-63 235.00
IUUC-Yli-134365 Yarrowia lipolytica 42.95 8.00e-63 232.00
IUUC-Zma-135941 Zea mays 36.24 2.00e-41 161.00
Created Date 25-Jun-2017

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