iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Ath-006627

UUCD1 version

UUC-ArT-01587

Ensembl Protein ID

AT5G41700.4

UniProt Accession

P35131; Q3E8J2; Q3E8J3; Q42308; Q43276; Q4TZ02; Q8LE19; UBC8_ARATH

Genbank Protein ID

CAA78713.1; AAY44848.1; BAB11476.1; AED94711.1; AED94712.1; AED94713.1; AED94714.1; AED94715.1; AAG40361.1; AAL34248.1; AAK44072.1; AAK96786.1; AAL15262.1; AAL66929.1; AAM62889.1; CAA85527.1; CAA79036.1

Protein Name

Ubiquitin-conjugating enzyme E2 8; E2 ubiquitin-conjugating enzyme 8; UBCAT4A; Ubiquitin carrier protein 8; Ubiquitin-conjugating enzyme E2-17 kDa 8; Ubiquitin-protein ligase 8

Genbank Nucleotide ID

Z14989; DQ027022; AB005233; CP002688; CP002688; CP002688; CP002688; CP002688; AF325009; AY063074; AF370257; AY054595; AY057631; AY072514; AY085670; Z37225; Z17692

Gene Name

UBC8; UBC4A; At5g41700; MBK23.24

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
AT5G41700	AT5G41700.3	AT5G41700.3
AT5G41700	AT5G41700.5	AT5G41700.5
AT5G41700	AT5G41700.4	AT5G41700.4

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
Protein-protein Interaction
iRefIndex	PINA	HINT
Protein 3D Structure
PDB	MMDB
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E2/UBC	E2_UBC	18753285; 20682837; 20147494; 18643997; 12028569; 21203445; 16339806

Classification

Family	E-value	Score	Start	End
E2/UBC	2.50e-54	183.7	5	140
UBD/UBC-like/UBD_UBC	7.30e-72	240.8	5	143

Active Site

Position(s)	Description	Evidence
85	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00388, ECO:0000255\|PROSITE-ProRule:PRU10133}

Domain Profile

E2/UBC

   S: 1    Rlkkelkelekdppegi.sakpvdesdltewevlilGpedtpYeggvFkleiefpedYPfkPPkvkfltkifhPnvyenGkvClsilkeeekWspals 97
           R+ kelk+l+kdpp+++ +a+pv e d+++w+++i+Gp+++pY ggvF ++i+fp+dYPfkPPkv f tk+fhPn+++nG++Cl+ilk  e+Wspal+
   Q: 5    RILKELKDLQKDPPTSCiFAGPVAE-DMFHWQATIMGPAESPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILK--EQWSPALT 99
           899***********87626788888.9************************************************************9..******** PP
   S: 98   vesvllsiqsllaepnpesplneeaaellkknreeykkkvr 138
           +++vllsi+sll++pnp++pl  e+a+++k++r++y+ ++r
   Q: 100  ISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEATAR 140
           *************************************9987 PP

UBD/UBC-like/UBD_UBC

   S: 1    rikkelkdllrdeeaqi.sadlvdddlfelratiagppdspyeggvffltiklptdypfkppkvafitkiyhpninsngsicldilrsqwsaaltlsk 97
           ri kelkdl++d+++ +  a++v +d+f+++ati+gp +spy ggvf +ti++p dypfkppkvaf tk++hpninsngsicldil++qws+alt+sk
   Q: 5    RILKELKDLQKDPPTSCiFAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISK 102
           899************65279****************************************************************************** PP
   S: 98   vllslcslladaepddplvaeiariyktdkekykriarewt 138
           vlls+csll+d++pddplv+eia++yktd+ ky+ +ar+wt
   Q: 103  VLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEATARNWT 143
           ****************************************8 PP

Organism

Arabidopsis thaliana

Functional Description
(View)

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Protein Sequence
(Fasta)

MASKRILKEL KDLQKDPPTS CIFAGPVAED MFHWQATIMG PAESPYSGGV FLVTIHFPPD 60
YPFKPPKVAF RTKVFHPNIN SNGSICLDIL KEQWSPALTI SKVLLSICSL LTDPNPDDPL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CATCTGCAGG AGTGAATCGC AAAACCAAAT TTGGAGGATT CTTTCTGAGG TATCAAAGCT 60
TTCTCCTTTT GATTAACCCA CACGAGAAAA AGCTCTCTTC TTTTGTAGGA ATTCAAAAAG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR000608--UBQ-conjugat_E2
IPR023313--UBQ-conjugating_AS
IPR016135--UBQ-conjugating_enzyme/RWD

PROSITE

PS00183--UBIQUITIN_CONJUGAT_1
PS50127--UBIQUITIN_CONJUGAT_2

Pfam

PF00179--UQ_con

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0016740--F:transferase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0009960--P:endosperm development
GO:0016567--P:protein ubiquitination
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

ath:AT5G41700

Orthology

iUUCD ID

Species

Identity

E-value

Score

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence