iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

mRNA Expression
GEO	FFGED
Protein-protein Interaction
IID	iRefIndex	HINT	Mentha
Post-translational Modifications (PTMs)
CPLM	dbPAF	BioGRID
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
UBD/Alpha-Helix/UBA	UBA	15258615; 16179953; 22454508
ULD/UDP/UFD/UBX	UBX	15258615

Classification

Family	E-value	Score	Start	End
UBD/Alpha-Helix/UBA	3.00e-08	32.0	16	59
ULD/UDP/UFD/UBX	8.40e-37	124.1	427	569

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

UBD/Alpha-Helix/UBA

   S: 2    ededkleqlve.MGFdre...ealqaLraannnleaAveyLldg 41
           ++edkl++++  ++F++e   ++++ Lr+++++le A+ +++dg
   Q: 16   TEEDKLNEFQViTNFPPEdlpDVVRLLRNHGWQLEPALSRYFDG 59
           689************8888***********************97 PP

ULD/UDP/UFD/UBX

   S: 2    tte.qatlqiRlpdGkrlvrkFpssttleqiyqfve...yllvessdp...................................................... 45
           tt+ qatlq+R+++Gkr+v+kFps+ttl+qiyq+++   yl+v+ssdp                                                      
   Q: 427  TTGkQATLQFRTSSGKRFVKKFPSMTTLYQIYQSIGchiYLAVYSSDPaewsnalqdkirqlsadddmlcfkegqletatattieelghiinneltsfdler 528
           6777************************************************************************************************** PP
   S: 46   ......FeLvspfPrrtlkpeehmstlqeagLvpsgsllve 80
                 FeLvspfP++t++p+ehms++q+++L+p+gsllve
   Q: 529  gklefdFELVSPFPKYTVHPNEHMSVDQVPQLWPNGSLLVE 569
           ***************************************97 PP

Organism

Saccharomyces cerevisiae

Functional Description
(View)

Integral endoplasmic reticulum membrane protein that coordinates the assembly of the ER-associated protein degradation (ERAD) machinery at the ER membrane. Mediates binding of CDC48 to the E3 ubiquitin ligases SSM4/DOA10 and HRD1, and to ERAD substrates. Component of the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains. ERAD-C substrates are ubiquitinated through DOA10 in conjunction with the E2 ubiquitin-conjugating enzymes UBC6 and UBC7-CUE1. Also component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex and targeted to the proteasome.

Protein Sequence
(Fasta)

MPVVNHEDSE FHLSHTEEDK LNEFQVITNF PPEDLPDVVR LLRNHGWQLE PALSRYFDGE 60
WKGEPDQMGE PTQTSTPMAE TLVPPALGPR PLLFTASLPV VRPLPANFRN DFRTIGLNGR 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGCCAGTAG TCAATCATGA GGATAGTGAA TTTCACCTTT CGCACACAGA AGAGGACAAG 60
TTAAATGAGT TCCAGGTCAT CACGAATTTC CCCCCAGAAG ACTTGCCAGA TGTGGTGAGA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0256--Endoplasmic reticulum
KW-0472--Membrane
KW-1185--Reference proteome
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0833--Ubl conjugation pathway

Interpro

IPR001012--UBX_dom

Pfam

PF00789--UBX

SMART

SM00166--UBX

Gene Ontology

GO:0000837--C:Doa10p ubiquitin ligase complex
GO:0000839--C:Hrd1p ubiquitin ligase ERAD-L complex
GO:0030176--C:integral component of endoplasmic reticulum membrane
GO:0005887--C:integral component of plasma membrane
GO:0005811--C:lipid particle
GO:0030674--F:protein binding, bridging
GO:0034389--P:lipid particle organization
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0030433--P:ubiquitin-dependent ERAD pathway

KEGG

sce:YML013W

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Apl-004127	Anas platyrhynchos	23.63	1.00e-03	36.60
IUUC-Aca-005188	Anolis carolinensis	23.63	4.00e-03	34.70
IUUC-Ago-007923	Ashbya gossypii	34.48	2.00e-87	314.00
IUUC-Cfa-021201	Canis familiaris	22.03	4.10e-02	31.20
IUUC-Cpo-021475	Cavia porcellus	26.17	1.40e-01	28.90
IUUC-Fca-036575	Felis catus	25.47	2.50e-02	31.20
IUUC-Fal-037128	Ficedula albicollis	23.63	1.00e-03	36.60
IUUC-Gmo-038814	Gadus morhua	25.27	8.80e-02	31.20
IUUC-Gga-040826	Gallus gallus	23.63	1.00e-03	36.60
IUUC-Gac-042355	Gasterosteus aculeatus	33.33	7.10e-02	30.80
IUUC-Itr-047922	Ictidomys tridecemlineatus	63.16	3.00e-01	28.50
IUUC-Kpa-049172	Komagataella pastoris	25.12	5.00e-34	137.00
IUUC-Lch-049605	Latimeria chalumnae	30.19	2.80e-02	29.60
IUUC-Lpe-050860	Leersia perrieri	27.71	2.80e-01	30.00
IUUC-Laf-054375	Loxodonta africana	22.03	2.30e-02	31.20
IUUC-Meu-056611	Macropus eugenii	20.22	2.00e-01	28.90
IUUC-Mga-059400	Meleagris gallopavo	23.63	1.00e-03	36.20
IUUC-Mpu-065713	Mustela putorius furo	22.10	2.20e-02	32.00
IUUC-Mlu-067971	Myotis lucifugus	31.36	3.00e-03	33.50
IUUC-Olo-080405	Oryza longistaminata	30.43	3.40e-01	27.30
IUUC-Osa-085462	Oryza sativa	48.15	4.70e-02	30.80
IUUC-Pva-102534	Pteropus vampyrus	25.47	6.60e-02	30.40
IUUC-Rno-105127	Rattus norvegicus	26.17	7.30e-02	30.40
IUUC-Sha-106667	Sarcophilus harrisii	23.03	2.40e-02	31.60
IUUC-Tgu-116495	Taeniopygia guttata	23.63	1.00e-03	36.60
IUUC-Tru-118034	Takifugu rubripes	34.69	2.90e-02	32.30

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence