iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-045854

UUCD1 version

UUC-HoS-00040

Ensembl Protein ID

ENSP00000465676.1

UniProt Accession

O75928; O75927; Q96BT5; Q96KE3; PIAS2_HUMAN

Genbank Protein ID

AAC36704.1; AAC36705.1; AAK48938.1; AAH15190.1

Protein Name

E3 SUMO-protein ligase PIAS2; Androgen receptor-interacting protein 3; DAB2-interacting protein; Msx-interacting zinc finger protein; PIAS-NY protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2

Genbank Nucleotide ID

AF077953; AF077954; AF361054; AC090241

Gene Name

PIAS2; PIASX

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000078043.15	ENST00000585916.5	ENSP00000465676.1
ENSG00000078043.15	ENST00000398654.7	ENSP00000381648.3
ENSG00000078043.15	ENST00000324794.11	ENSP00000317163.6
ENSG00000078043.15	ENST00000592212.5	ENSP00000479025.1
ENSG00000078043.15	ENST00000586953.1	ENSP00000468166.1
ENSG00000078043.15	ENST00000587810.1	ENSP00000468017.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
miRWalk	TargetScan	RepTar	SomamiR
miRcode	RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Protein 3D Structure
PDB	MMDB
Disease-associated Information
GWASdb	OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	UniProt	BioGRID	mUbiSiDa
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/RING/RING	RING	17855618; 16148010; 18218142

Classification

Family	E-value	Score	Start	End
E3 activity/RING/RING	0.013	17.7	347	389

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 2    aiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           ++ + ++  + + + C H+ ++d    +++++n+++++++CP+C+
   Q: 347  PLGKMRLTIPCRAVTCTHLQCFDAA--LYLQMNEKKPTWICPVCD 389
           4444445454556678888888884..35899************5 PP

Organism

Homo sapiens

Functional Description
(View)

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.

Protein Sequence
(Fasta)

MADFEELRNM VSSFRVSELQ VLLGFAGRNK SGRKHDLLMR ALHLLKSGCS PAVQIKIREL 60
YRRRYPRTLE GLSDLSTIKS SVFSLDGGSS PVEPDLAVAG IHSLPSTSVT PHSPSSPVGS 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

AGAACTCTTT GCAGTCAAAC CTACCCAACC CATCCAGGAG CTGCTACACA TTGAGGAAGG 60
GGCAGAATGA ATGCTGGGAA GCAACTACAA AGAACACTGC ACGTAAGTTT CAGTGGGGCT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-1017--Isopeptide bond
KW-0436--Ligase
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR027228--PIAS2
IPR023321--PINIT
IPR003034--SAP_dom
IPR004181--Znf_MIZ

PROSITE

PS51466--PINIT
PS50800--SAP
PS51044--ZF_SP_RING

Pfam

PF14324--PINIT
PF02891--zf-MIZ

SMART

SM00513--SAP

Gene Ontology

GO:0016607--C:nuclear speck
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0016605--C:PML body
GO:0050681--F:androgen receptor binding
GO:0003677--F:DNA binding
GO:0016874--F:ligase activity
GO:0061665--F:SUMO ligase activity
GO:0019789--F:SUMO transferase activity
GO:0003713--F:transcription coactivator activity
GO:0008134--F:transcription factor binding
GO:0008270--F:zinc ion binding
GO:0030521--P:androgen receptor signaling pathway
GO:0060766--P:negative regulation of androgen receptor signaling pathway
GO:0043433--P:negative regulation of sequence-specific DNA binding transcription factor activity
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0045893--P:positive regulation of transcription, DNA-templated
GO:0016925--P:protein sumoylation
GO:0045667--P:regulation of osteoblast differentiation
GO:0006351--P:transcription, DNA-templated

KEGG

hsa:9063

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002262	Ailuropoda melanoleuca	97.75	0.00e+00	1023.00
IUUC-Apl-004116	Anas platyrhynchos	93.11	0.00e+00	939.00
IUUC-Aca-004464	Anolis carolinensis	91.28	0.00e+00	961.00
IUUC-Acl-008204	Aspergillus clavatus	33.51	4.00e-28	119.00
IUUC-Afl-008672	Aspergillus flavus	37.36	2.00e-29	124.00
IUUC-Afu-008758	Aspergillus fumigatus	30.95	4.00e-30	125.00
IUUC-Ani-009389	Aspergillus nidulans	34.90	1.00e-26	114.00
IUUC-Ang-009590	Aspergillus niger	31.72	9.00e-29	121.00
IUUC-Aor-009912	Aspergillus oryzae	40.60	3.00e-25	109.00
IUUC-Ate-010496	Aspergillus terreus	37.08	9.00e-30	124.00
IUUC-Ame-010633	Astyanax mexicanus	71.90	0.00e+00	792.00
IUUC-Bgr-012177	Blumeria graminis	28.49	5.00e-24	105.00
IUUC-Bta-013489	Bos taurus	96.30	0.00e+00	1028.00
IUUC-Cja-019247	Callithrix jacchus	99.68	0.00e+00	1038.00
IUUC-Cfa-021020	Canis familiaris	98.86	0.00e+00	1021.00
IUUC-Csa-023988	Chlorocebus sabaeus	99.64	0.00e+00	1014.00
IUUC-Cho-024816	Choloepus hoffmanni	87.44	0.00e+00	846.00
IUUC-Cne-026824	Cryptococcus neoformans	28.40	2.00e-19	91.70
IUUC-Dre-027615	Danio rerio	72.40	0.00e+00	799.00
IUUC-Dno-028983	Dasypus novemcinctus	98.64	0.00e+00	948.00
IUUC-Dor-030560	Dipodomys ordii	79.77	0.00e+00	715.00
IUUC-Dse-031254	Dothistroma septosporum	31.86	2.00e-27	117.00
IUUC-Ete-032543	Echinops telfairi	80.91	0.00e+00	791.00
IUUC-Eca-033818	Equus caballus	99.35	0.00e+00	1015.00
IUUC-Eeu-035256	Erinaceus europaeus	84.67	0.00e+00	842.00
IUUC-Fca-035422	Felis catus	98.86	0.00e+00	1017.00
IUUC-Fal-037641	Ficedula albicollis	91.00	0.00e+00	1006.00
IUUC-Gga-040849	Gallus gallus	91.64	0.00e+00	996.00
IUUC-Ggo-044666	Gorilla gorilla	98.87	0.00e+00	1021.00
IUUC-Hvu-047670	Hordeum vulgare	38.46	5.00e-17	82.80
IUUC-Lch-050424	Latimeria chalumnae	74.22	0.00e+00	774.00
IUUC-Loc-052343	Lepisosteus oculatus	77.62	0.00e+00	816.00
IUUC-Lma-053196	Leptosphaeria maculans	34.46	3.00e-29	123.00
IUUC-Laf-054247	Loxodonta africana	96.93	0.00e+00	996.00
IUUC-Mcc-055103	Macaca mulatta	99.84	0.00e+00	1021.00
IUUC-Meu-056533	Macropus eugenii	68.29	0.00e+00	695.00
IUUC-Mla-058882	Melampsora laricipopulina	32.66	9.00e-24	105.00
IUUC-Mmr-060682	Microcebus murinus	98.87	0.00e+00	1055.00
IUUC-Mdo-062100	Monodelphis domestica	89.10	0.00e+00	996.00
IUUC-Mmu-063248	Mus musculus	98.07	0.00e+00	1040.00
IUUC-Mpu-066494	Mustela putorius furo	98.53	0.00e+00	1020.00
IUUC-Mlu-067038	Myotis lucifugus	98.06	0.00e+00	1011.00
IUUC-Nfi-068586	Neosartorya fischeri	30.95	1.00e-30	127.00
IUUC-Nle-070060	Nomascus leucogenys	99.45	0.00e+00	994.00
IUUC-Opr-070251	Ochotona princeps	78.79	0.00e+00	823.00
IUUC-Ont-071330	Oreochromis niloticus	74.71	0.00e+00	656.00
IUUC-Oan-073028	Ornithorhynchus anatinus	95.61	0.00e+00	1029.00
IUUC-Ocu-074400	Oryctolagus cuniculus	97.07	0.00e+00	1032.00
IUUC-Ola-087125	Oryzias latipes	72.56	1.00e-180	626.00
IUUC-Oga-089150	Otolemur garnettii	96.46	0.00e+00	1011.00
IUUC-Oar-089866	Ovis aries	96.76	0.00e+00	1026.00
IUUC-Ptr-090464	Pan troglodytes	99.52	0.00e+00	1038.00
IUUC-Pan-091938	Papio anubis	99.84	0.00e+00	1041.00
IUUC-Psi-093868	Pelodiscus sinensis	95.37	0.00e+00	979.00
IUUC-Pno-094862	Phaeosphaeria nodorum	30.85	9.00e-31	128.00
IUUC-Pfo-097230	Poecilia formosa	71.30	0.00e+00	631.00
IUUC-Pab-097699	Pongo abelii	99.68	0.00e+00	1038.00
IUUC-Pca-101088	Procavia capensis	88.91	0.00e+00	894.00
IUUC-Pgr-103580	Puccinia graminis	34.57	3.00e-24	107.00
IUUC-Ptt-103870	Puccinia triticina	32.09	1.00e-25	110.00
IUUC-Pte-104331	Pyrenophora teres	29.35	1.00e-29	124.00
IUUC-Pyt-104673	Pyrenophora triticirepentis	35.59	2.00e-28	120.00
IUUC-Sha-107323	Sarcophilus harrisii	91.17	0.00e+00	1028.00
IUUC-Smo-109174	Selaginella moellendorffii	33.04	1.00e-14	75.50
IUUC-Sar-113273	Sorex araneus	97.56	0.00e+00	1097.00
IUUC-Tgu-116644	Taeniopygia guttata	90.51	0.00e+00	990.00
IUUC-Tru-117897	Takifugu rubripes	70.48	0.00e+00	630.00
IUUC-Tsy-119019	Tarsius syrichta	87.91	0.00e+00	878.00
IUUC-Tni-120384	Tetraodon nigroviridis	71.14	2.00e-179	622.00
IUUC-Tre-122003	Trichoderma reesei	31.76	6.00e-28	119.00
IUUC-Tme-126880	Tuber melanosporum	29.29	8.00e-28	118.00
IUUC-Tbe-127942	Tupaia belangeri	81.56	8.00e-139	487.00
IUUC-Ttr-128980	Tursiops truncatus	78.47	0.00e+00	706.00
IUUC-Vda-129985	Verticillium dahliae	35.65	1.00e-30	127.00
IUUC-Vpa-130725	Vicugna pacos	97.90	0.00e+00	850.00
IUUC-Xtr-132733	Xenopus tropicalis	78.18	0.00e+00	887.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence