iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000032216.14	ENSMUST00000034740.14	ENSMUSP00000034740.7
ENSMUSG00000032216.14	ENSMUST00000184450.7	ENSMUSP00000138983.1
ENSMUSG00000032216.14	ENSMUST00000183375.7	ENSMUSP00000138852.1
ENSMUSG00000032216.14	ENSMUST00000184020.7	ENSMUSP00000139297.1

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	circBase	miRTarBase	microRNA
TRANSFAC	RepTar	RAID2
Protein-protein Interaction
IID	PINA	HINT	Mentha
Protein 3D Structure
PDB	MMDB
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
Phospho.ELM	UniProt	PHOSIDA	mUbiSiDa
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	19953087; 11717310

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	3.00e-133	445.5	554	884

Active Site

Position(s)	Description	Evidence
854	Glycyl thioester intermediate	N/A

Domain Profile

E3 activity/HECT

   S: 3    sdlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldlp 91
           + lk+rl++ef gE+gld+gGv+re+++l+ske++np++glf+ys++d++tlq+np+sgl+n++hl++F+f+Gr+ g+a+++g+lld +
   Q: 554  DLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGF 642
           56899************************************************************************************ PP
   S: 92   lsksfyklllgkklsledleevdpelykslkeikenaesdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvvey 180
           + ++fyk++l+k ++l+d+e+vd+e+y sl +i+en   d +e  ldl+f ++ee++g+t+++eLk+gG++i Vtn+Nk+eY+ lv+++
   Q: 643  FIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILEN---DPTE--LDLRFIIDEELFGQTHQHELKTGGSEIVVTNKNKKEYIYLVIQW 726
           **********************************99...6666..7******************************************* PP
   S: 181  rlkegikeqveafreGfneviplellslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFvT 269
           r+ ++i++q++af+eGf e+ip++l+++F+e+elell+cG  ++dv+d++++tkyk+gy+ ++++i+wFw+++ +++se+r ++LqFvT
   Q: 727  RFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSMNHQVIHWFWKAVWMMDSEKRIRLLQFVT 815
           ***************************************************************************************** PP
   S: 270  gssrlPlgGfaelk....prlfvirkdasddrlPssnTCfnyLkLPeYsskeilkekLlyAieetkgFe 334
           g+sr+P++Gfael     p++f++++++++d+lP+++TCfn+L+LP+Y+s ++l +kL++Aie+t+gF+
   Q: 816  GTSRVPMNGFAELYgsngPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFD 884
           ********************************************************************8 PP

Organism

Mus musculus

Functional Description
(View)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (By similarity). Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Involved in ubiquitination of ERBB4 intracellular domain E4ICD1 (PubMed:19193720). Predominantly involved in ubiquitination of membrane bound forms of ERBB4 rather than processed precursors and intermediate membrane-anchored 80 kDa fragments (m80HER4), with a lesser role in ubiquitination of ERBB4 intracellular domain E4ICD1 (PubMed:19047365). Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2 (By similarity). Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (By similarity).

Protein Sequence
(Fasta)

MSSDMAADES EAPVLSEDEV WEFCLDKTED GGGSPGSDVT DTCEPPCGCW ELNPNSLEEE 60
HVLFTADPYL ELHNDDTRVV RVKVIAGIGL AKKDILGASD PYVRVTLYDP MSGILTSVQT 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CCACGCTTGC TCCAACCCAG CCCCCCAAGC CTCGCGCGCA GCCGCAGAGC GGGTCCCGCC 60
CCTTCTCGCC GCGCGCCCCT GCACCCCAGC GCGCTCCCGG AGTGGGCCTC CTCCGCCGCA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0472--Membrane
KW-0524--Neurogenesis
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR000008--C2_dom
IPR000569--HECT_dom
IPR001202--WW_dom

PROSITE

PS50004--C2
PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2

Pfam

PF00168--C2
PF00632--HECT
PF00397--WW

SMART

SM00239--C2
SM00119--HECTc
SM00456--WW

Gene Ontology

GO:0005938--C:cell cortex
GO:0000785--C:chromatin
GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0043197--C:dendritic spine
GO:0070062--C:extracellular exosome
GO:0005794--C:Golgi apparatus
GO:0016020--C:membrane
GO:0048471--C:perinuclear region of cytoplasm
GO:0005886--C:plasma membrane
GO:0000151--C:ubiquitin ligase complex
GO:0035255--F:ionotropic glutamate receptor binding
GO:0050815--F:phosphoserine binding
GO:0050816--F:phosphothreonine binding
GO:0070064--F:proline-rich region binding
GO:0019871--F:sodium channel inhibitor activity
GO:0061630--F:ubiquitin protein ligase activity
GO:0002250--P:adaptive immune response
GO:0048514--P:blood vessel morphogenesis
GO:0044111--P:development involved in symbiotic interaction
GO:0003197--P:endocardial cushion development
GO:0042921--P:glucocorticoid receptor signaling pathway
GO:2000650--P:negative regulation of sodium ion transmembrane transporter activity
GO:0010766--P:negative regulation of sodium ion transport
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0010768--P:negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage
GO:0030948--P:negative regulation of vascular endothelial growth factor receptor signaling pathway
GO:0007528--P:neuromuscular junction development
GO:0031175--P:neuron projection development
GO:0003151--P:outflow tract morphogenesis
GO:0046824--P:positive regulation of nucleocytoplasmic transport
GO:0014068--P:positive regulation of phosphatidylinositol 3-kinase signaling
GO:0045732--P:positive regulation of protein catabolic process
GO:0050847--P:progesterone receptor signaling pathway
GO:0070534--P:protein K63-linked ubiquitination
GO:0006513--P:protein monoubiquitination
GO:0006622--P:protein targeting to lysosome
GO:0016567--P:protein ubiquitination
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0032801--P:receptor catabolic process
GO:0031623--P:receptor internalization
GO:0048814--P:regulation of dendrite morphogenesis
GO:0042391--P:regulation of membrane potential
GO:1901016--P:regulation of potassium ion transmembrane transporter activity
GO:0050807--P:regulation of synapse organization
GO:0042110--P:T cell activation
GO:0019089--P:transmission of virus
GO:0043162--P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

KEGG

mmu:17999

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001201	Ailuropoda melanoleuca	78.70	0.00e+00	1230.00
IUUC-Bta-012453	Bos taurus	77.16	0.00e+00	1209.00
IUUC-Cja-019034	Callithrix jacchus	76.13	0.00e+00	1081.00
IUUC-Cfa-020258	Canis familiaris	79.20	0.00e+00	1179.00
IUUC-Cpo-021648	Cavia porcellus	77.04	0.00e+00	1288.00
IUUC-Csa-023334	Chlorocebus sabaeus	79.66	0.00e+00	1340.00
IUUC-Cin-025157	Ciona intestinalis	52.71	0.00e+00	847.00
IUUC-Dre-027924	Danio rerio	62.03	0.00e+00	907.00
IUUC-Dno-029159	Dasypus novemcinctus	72.88	0.00e+00	1028.00
IUUC-Ete-032409	Echinops telfairi	71.49	0.00e+00	941.00
IUUC-Eca-033441	Equus caballus	80.14	0.00e+00	1313.00
IUUC-Eeu-034546	Erinaceus europaeus	65.66	0.00e+00	911.00
IUUC-Fca-036384	Felis catus	77.78	0.00e+00	1208.00
IUUC-Fal-037547	Ficedula albicollis	72.25	0.00e+00	1261.00
IUUC-Gmo-039679	Gadus morhua	63.53	0.00e+00	1085.00
IUUC-Gga-040524	Gallus gallus	72.83	0.00e+00	1254.00
IUUC-Hsa-046479	Homo sapiens	79.33	0.00e+00	1220.00
IUUC-Itr-048383	Ictidomys tridecemlineatus	86.73	0.00e+00	1177.00
IUUC-Mcc-055585	Macaca mulatta	78.83	0.00e+00	1208.00
IUUC-Meu-056897	Macropus eugenii	92.68	5.00e-164	572.00
IUUC-Mga-059362	Meleagris gallopavo	66.67	0.00e+00	1230.00
IUUC-Mmr-061061	Microcebus murinus	78.86	0.00e+00	1230.00
IUUC-Mpu-066567	Mustela putorius furo	79.64	0.00e+00	1372.00
IUUC-Mlu-068148	Myotis lucifugus	78.48	0.00e+00	1194.00
IUUC-Nle-069225	Nomascus leucogenys	79.20	0.00e+00	1208.00
IUUC-Oan-073019	Ornithorhynchus anatinus	74.47	0.00e+00	1226.00
IUUC-Ocu-074409	Oryctolagus cuniculus	78.62	0.00e+00	1225.00
IUUC-Oga-088241	Otolemur garnettii	71.84	0.00e+00	1051.00
IUUC-Oar-089797	Ovis aries	79.18	0.00e+00	1318.00
IUUC-Ptr-091629	Pan troglodytes	76.53	0.00e+00	1077.00
IUUC-Psi-093618	Pelodiscus sinensis	72.14	0.00e+00	1113.00
IUUC-Pab-098394	Pongo abelii	79.77	0.00e+00	1340.00
IUUC-Pca-100568	Procavia capensis	68.58	0.00e+00	947.00
IUUC-Rno-106095	Rattus norvegicus	96.29	0.00e+00	1712.00
IUUC-Sha-107370	Sarcophilus harrisii	68.24	0.00e+00	1095.00
IUUC-Tgu-117474	Taeniopygia guttata	73.28	0.00e+00	1276.00
IUUC-Tru-117675	Takifugu rubripes	59.60	0.00e+00	1077.00
IUUC-Tbe-127408	Tupaia belangeri	73.00	0.00e+00	947.00
IUUC-Ttr-128437	Tursiops truncatus	70.76	0.00e+00	955.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence