iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-046146

UUCD1 version

UUC-HoS-00039

Ensembl Protein ID

ENSP00000249636.6

UniProt Accession

O75925; B2RB67; B3KSY9; C5J4B4; Q147X4; Q99751; Q9UN02; PIAS1_HUMAN

Genbank Protein ID

AAC36702.1; AAD49722.1; ACR77525.1; BAG52901.1; BAG37114.1; AAI18588.1; AAI21798.1; AAB58488.1

Protein Name

E3 SUMO-protein ligase PIAS1; DEAD/H box-binding protein 1; Gu-binding protein; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein

Genbank Nucleotide ID

AF077951; AF167160; FJ997900; AK094641; AK314515; AC107871; BC121797; U78524

Gene Name

PIAS1; DDXBP1

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000033800.13	ENST00000249636.10	ENSP00000249636.6
ENSG00000033800.13	ENST00000564915.5	ENSP00000456721.1
ENSG00000033800.13	ENST00000562190.1	ENSP00000457698.1
ENSG00000033800.13	ENST00000545237.1	ENSP00000438574.1
ENSG00000033800.13	ENST00000563996.1	ENSP00000456558.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	circBase	circRNADb	CircNet
miRTarBase	microRNA	TRANSFAC	miRWalk
TargetScan	RepTar	miRNAMap	SomamiR
miRcode	RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Protein 3D Structure
PDB	MMDB
Disease-associated Information
GWASdb	OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
HPRD	Phospho.ELM	UniProt	PHOSIDA
BioGRID	mUbiSiDa
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/RING/RING	RING	20711444; 18755518; 19557165; 18511503; 17218271; 17066076; 14701874; 14500712; 16144832; 15961505; 15337742; 15280358; 20209145; 19843541; 20127678; 16678795; 16563226; 16568089; 19782030; 17509614; 15582666; 17938210; 18218142; 17101784; 15572666; 21467194; 17440973; 14644436; 24872413

Classification

Family	E-value	Score	Start	End
E3 activity/RING/RING	0.026	16.7	335	378

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C++ + ++  + + l C H+ ++d    +  ++n++++++ CP+C+
   Q: 335  CPLGKMRLTIPCRALTCSHLQCFDAT-LY-IQMNEKKPTWVCPVCD 378
           55555555556778899999999995.33.6899***********5 PP

Organism

Homo sapiens

Functional Description
(View)

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity).

Protein Sequence
(Fasta)

MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS PAVQMKIKEL 60
YRRRFPQKIM TPADLSIPNV HSSPMPATLS PSTIPQLTYD GHPASSPLLP VSLLGPKHEL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGGGCTTCGA GCGCCCTGAG CGGGGCGGAG GCTAGAGGCG GGCTGGGAAG GTGGAGGGGC 60
GGGCCGGGGC GGGGCCAGGC CGGCTAGAGG GGCGGGTCTA GCGGCGGCCC CCGGCGAAGT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-1017--Isopeptide bond
KW-0436--Ligase
KW-0479--Metal-binding
KW-0488--Methylation
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR027227--PIAS1
IPR023321--PINIT
IPR003034--SAP_dom
IPR004181--Znf_MIZ

PROSITE

PS51466--PINIT
PS50800--SAP
PS51044--ZF_SP_RING

Pfam

PF14324--PINIT
PF02891--zf-MIZ

SMART

SM00513--SAP

Gene Ontology

GO:0016607--C:nuclear speck
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0016605--C:PML body
GO:0050681--F:androgen receptor binding
GO:0003677--F:DNA binding
GO:0019899--F:enzyme binding
GO:0016874--F:ligase activity
GO:0061665--F:SUMO ligase activity
GO:0019789--F:SUMO transferase activity
GO:0003713--F:transcription coactivator activity
GO:0003714--F:transcription corepressor activity
GO:0008270--F:zinc ion binding
GO:0030521--P:androgen receptor signaling pathway
GO:0045444--P:fat cell differentiation
GO:0000082--P:G1/S transition of mitotic cell cycle
GO:0007259--P:JAK-STAT cascade
GO:0043066--P:negative regulation of apoptotic process
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0032436--P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0033235--P:positive regulation of protein sumoylation
GO:0051152--P:positive regulation of smooth muscle cell differentiation
GO:0045893--P:positive regulation of transcription, DNA-templated
GO:0016925--P:protein sumoylation
GO:0065004--P:protein-DNA complex assembly
GO:0042127--P:regulation of cell proliferation
GO:0060334--P:regulation of interferon-gamma-mediated signaling pathway
GO:0007283--P:spermatogenesis
GO:0006351--P:transcription, DNA-templated
GO:0008542--P:visual learning

KEGG

hsa:8554

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001984	Ailuropoda melanoleuca	99.07	0.00e+00	1185.00
IUUC-Apl-003376	Anas platyrhynchos	93.86	0.00e+00	1179.00
IUUC-Aca-004444	Anolis carolinensis	86.11	0.00e+00	1086.00
IUUC-Aly-006507	Arabidopsis lyrata	35.48	2.00e-14	75.10
IUUC-Ame-011812	Astyanax mexicanus	74.66	0.00e+00	809.00
IUUC-Bta-012457	Bos taurus	99.08	0.00e+00	1201.00
IUUC-Bci-013700	Botrytis cinerea	29.49	2.00e-29	123.00
IUUC-Bol-015250	Brassica oleracea	27.23	4.00e-17	83.60
IUUC-Cel-018231	Caenorhabditis elegans	36.94	3.00e-49	190.00
IUUC-Cfa-020481	Canis familiaris	99.69	0.00e+00	1194.00
IUUC-Cpo-021940	Cavia porcellus	98.31	0.00e+00	1207.00
IUUC-Csa-023964	Chlorocebus sabaeus	100.00	0.00e+00	1197.00
IUUC-Cho-024812	Choloepus hoffmanni	81.78	0.00e+00	727.00
IUUC-Csv-026329	Ciona savignyi	38.51	1.00e-78	288.00
IUUC-Cme-027209	Cyanidioschyzon merolae	33.11	3.00e-15	77.00
IUUC-Dre-027526	Danio rerio	75.98	0.00e+00	894.00
IUUC-Dno-029550	Dasypus novemcinctus	99.38	0.00e+00	1192.00
IUUC-Dor-030325	Dipodomys ordii	83.81	0.00e+00	721.00
IUUC-Dme-032018	Drosophila melanogaster	47.04	2.00e-100	359.00
IUUC-Ete-032366	Echinops telfairi	86.23	0.00e+00	681.00
IUUC-Eca-033639	Equus caballus	99.22	0.00e+00	1172.00
IUUC-Eeu-034994	Erinaceus europaeus	86.65	0.00e+00	676.00
IUUC-Fca-035986	Felis catus	99.22	0.00e+00	1172.00
IUUC-Fal-036926	Ficedula albicollis	94.47	0.00e+00	1123.00
IUUC-Gmo-039058	Gadus morhua	71.19	0.00e+00	827.00
IUUC-Gga-040755	Gallus gallus	93.42	0.00e+00	1179.00
IUUC-Gac-042204	Gasterosteus aculeatus	72.28	0.00e+00	842.00
IUUC-Ggo-044494	Gorilla gorilla	97.36	0.00e+00	1145.00
IUUC-Itr-048994	Ictidomys tridecemlineatus	98.91	0.00e+00	1168.00
IUUC-Lch-049611	Latimeria chalumnae	83.95	0.00e+00	1034.00
IUUC-Loc-052851	Lepisosteus oculatus	77.09	0.00e+00	854.00
IUUC-Laf-053486	Loxodonta africana	97.52	0.00e+00	1149.00
IUUC-Mcc-055123	Macaca mulatta	100.00	0.00e+00	1197.00
IUUC-Meu-056556	Macropus eugenii	68.82	0.00e+00	755.00
IUUC-Mga-059790	Meleagris gallopavo	96.21	0.00e+00	1063.00
IUUC-Mmr-060785	Microcebus murinus	99.38	0.00e+00	1178.00
IUUC-Mdo-061916	Monodelphis domestica	97.51	0.00e+00	1181.00
IUUC-Mmu-063170	Mus musculus	98.00	0.00e+00	1152.00
IUUC-Mpu-066609	Mustela putorius furo	99.69	0.00e+00	1194.00
IUUC-Mlu-067164	Myotis lucifugus	97.83	0.00e+00	1154.00
IUUC-Nle-070004	Nomascus leucogenys	100.00	0.00e+00	1197.00
IUUC-Opr-070738	Ochotona princeps	78.23	0.00e+00	810.00
IUUC-Ont-071607	Oreochromis niloticus	76.52	0.00e+00	840.00
IUUC-Oan-073567	Ornithorhynchus anatinus	90.06	0.00e+00	1141.00
IUUC-Ocu-073833	Oryctolagus cuniculus	98.62	0.00e+00	1153.00
IUUC-Ola-086471	Oryzias latipes	72.73	0.00e+00	879.00
IUUC-Oga-088527	Otolemur garnettii	98.46	0.00e+00	1157.00
IUUC-Oar-089470	Ovis aries	98.91	0.00e+00	1184.00
IUUC-Ptr-091086	Pan troglodytes	100.00	0.00e+00	1197.00
IUUC-Pan-092236	Papio anubis	100.00	0.00e+00	1197.00
IUUC-Psi-093900	Pelodiscus sinensis	92.70	0.00e+00	1149.00
IUUC-Pma-094693	Petromyzon marinus	65.18	3.00e-177	615.00
IUUC-Pfo-096236	Poecilia formosa	75.38	0.00e+00	860.00
IUUC-Pab-098400	Pongo abelii	100.00	0.00e+00	1196.00
IUUC-Pva-102327	Pteropus vampyrus	90.63	0.00e+00	1054.00
IUUC-Rno-105980	Rattus norvegicus	98.16	0.00e+00	1183.00
IUUC-Spo-108339	Schizosaccharomyces pombe	28.57	4.00e-28	120.00
IUUC-Stu-112120	Solanum tuberosum	35.71	2.00e-17	84.00
IUUC-Sar-113126	Sorex araneus	97.83	0.00e+00	1189.00
IUUC-Sbi-114542	Sorghum bicolor	34.68	2.00e-16	81.30
IUUC-Ssc-115394	Sus scrofa	99.46	0.00e+00	1070.00
IUUC-Tgu-117164	Taeniopygia guttata	92.76	0.00e+00	877.00
IUUC-Tru-117960	Takifugu rubripes	76.38	0.00e+00	834.00
IUUC-Tsy-119069	Tarsius syrichta	97.36	0.00e+00	1131.00
IUUC-Tni-120583	Tetraodon nigroviridis	73.64	0.00e+00	825.00
IUUC-Tbe-127466	Tupaia belangeri	88.40	3.00e-151	529.00
IUUC-Ttr-128717	Tursiops truncatus	92.17	0.00e+00	1058.00
IUUC-Vpa-130624	Vicugna pacos	95.08	0.00e+00	1074.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence